Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,916,830 C→A 100% D71Y (GAT→TAT)  rsmG ← 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,916,8300CA99.5% 670.6 / ‑4.3 205D71Y (GAT→TAT) rsmG16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (103/100);  total (104/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

GGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTG  >  CP009273/3916685‑3916974
                                                                                                                                                 |                                                                                                                                                
ggTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGa                                                                                                                                                <  1:814712/148‑1 (MQ=255)
 gTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGAt                                                                                                                                               >  1:1445296/1‑148 (MQ=255)
 gTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGAt                                                                                                                                               >  1:3191065/1‑148 (MQ=255)
   tCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATaa                                                                                                                                             <  1:908174/148‑1 (MQ=255)
   tCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATaa                                                                                                                                             <  2:2537835/148‑1 (MQ=255)
    cAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATaaa                                                                                                                                            <  1:143942/148‑1 (MQ=255)
        atTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGt                                                                                                                                        >  1:1407161/1‑148 (MQ=255)
          tctcCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTc                                                                                                                                      >  1:685051/1‑148 (MQ=255)
           ctcCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCa                                                                                                                                     >  1:1350904/1‑148 (MQ=255)
            tcCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCAc                                                                                                                                    >  1:3439723/1‑148 (MQ=255)
            tcCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCAc                                                                                                                                    >  1:199996/1‑148 (MQ=255)
             ccAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCAcc                                                                                                                                   >  2:2492434/1‑148 (MQ=255)
               aGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCtt                                                                                                                                 >  1:3348449/1‑148 (MQ=255)
                 tttAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGc                                                                                                                               >  1:2456027/1‑148 (MQ=255)
                 tttAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGc                                                                                                                               >  1:446536/1‑148 (MQ=255)
                  ttAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCa                                                                                                                              >  2:1206968/1‑148 (MQ=255)
                   tAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAg                                                                                                                             >  1:464542/1‑148 (MQ=255)
                    aaGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGa                                                                                                                            >  2:2737122/1‑148 (MQ=255)
                      gCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATa                                                                                                                          >  2:1691372/1‑148 (MQ=255)
                       cTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATAc                                                                                                                         >  1:524155/1‑148 (MQ=255)
                       cTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATAc                                                                                                                         >  1:1009868/1‑148 (MQ=255)
                       cTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATAc                                                                                                                         >  1:2425262/1‑148 (MQ=255)
                         cATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACgg                                                                                                                       >  1:1188007/1‑148 (MQ=255)
                         cATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACgg                                                                                                                       <  2:879727/148‑1 (MQ=255)
                         cATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACgg                                                                                                                       <  2:3110317/148‑1 (MQ=255)
                         cATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACgg                                                                                                                       <  2:3124650/148‑1 (MQ=255)
                            gTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGc                                                                                                                    <  1:2626642/148‑1 (MQ=255)
                              tGCACCTGACGAAGGAAACTCACGCGTTTACCAAGGCTATCGAACAGAGTGAAATGGGCTTCAGGACTCTCGATAGATATTGGAATGCCTGGCAGTCCTGGTCCGTTGCCTACATAGATAAACCTTTCACCTTGCAGATACGGTGcca                                                                                                                  <  2:2803812/148‑1 (MQ=255)
                                  ccTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCcaccac                                                                                                              >  1:2521615/1‑148 (MQ=255)
                                      aCGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATg                                                                                                          <  2:2389424/148‑1 (MQ=255)
                                       cGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGc                                                                                                         >  1:2922373/1‑148 (MQ=255)
                                         aaGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTa                                                                                                       <  2:1274518/148‑1 (MQ=255)
                                         aaGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTa                                                                                                       <  2:204900/148‑1 (MQ=255)
                                               acgcacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCgaga                                                                                                 >  2:293606/1‑148 (MQ=255)
                                                cgcacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGaa                                                                                                >  1:3044970/1‑148 (MQ=255)
                                                cgcacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGaa                                                                                                >  2:3339673/1‑148 (MQ=255)
                                                cgcacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGaa                                                                                                <  1:2413456/148‑1 (MQ=255)
                                                  cacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAata                                                                                              <  2:12814/148‑1 (MQ=255)
                                                   acgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAatat                                                                                             <  1:1065915/148‑1 (MQ=255)
                                                    cgcgTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTTGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATg                                                                                            <  1:1806756/148‑1 (MQ=255)
                                                      cgTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGc                                                                                          >  2:215743/1‑148 (MQ=255)
                                                      cgTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGc                                                                                          >  2:215476/1‑148 (MQ=255)
                                                        tttACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGt                                                                                        >  1:2352143/1‑148 (MQ=255)
                                                         ttACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTa                                                                                       >  2:1873844/1‑148 (MQ=255)
                                                         ttACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTa                                                                                       <  2:446536/148‑1 (MQ=255)
                                                             cAAGGCTATCCAAGAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAg                                                                                   >  2:1540606/1‑148 (MQ=255)
                                                              aaGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGc                                                                                  >  1:2698300/1‑148 (MQ=255)
                                                                ggCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAt                                                                                >  2:2754984/1‑148 (MQ=255)
                                                                ggCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAt                                                                                >  2:95381/1‑148 (MQ=255)
                                                                 gCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAtc                                                                               >  1:57005/1‑148 (MQ=255)
                                                                   tATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAtctc                                                                             <  1:2583677/148‑1 (MQ=255)
                                                                   tATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAtctc                                                                             <  1:3172932/148‑1 (MQ=255)
                                                                    aTCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCa                                                                            >  1:2746315/1‑148 (MQ=255)
                                                                     tCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCAt                                                                           >  1:2862688/1‑148 (MQ=255)
                                                                     tCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCAt                                                                           <  2:570671/148‑1 (MQ=255)
                                                                       cAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTa                                                                         <  2:1230667/148‑1 (MQ=255)
                                                                          cAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGa                                                                      <  1:2110798/148‑1 (MQ=255)
                                                                          cAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGa                                                                      >  2:2145389/1‑148 (MQ=255)
                                                                          cAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGa                                                                      <  1:811225/148‑1 (MQ=255)
                                                                            gagTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATc                                                                    >  2:2711595/1‑148 (MQ=255)
                                                                            gagTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATc                                                                    >  1:3205810/1‑148 (MQ=255)
                                                                                    tGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCg                                                            <  2:1212507/148‑1 (MQ=255)
                                                                                        cTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGt                                                        >  1:1654868/1‑148 (MQ=255)
                                                                                        cTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGt                                                        <  2:678034/148‑1 (MQ=255)
                                                                                        cTTCAGGACGCACGAGAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGt                                                        <  2:3443359/148‑1 (MQ=255)
                                                                                           cAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAg                                                     >  2:1656941/1‑148 (MQ=255)
                                                                                             ggACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGt                                                   >  2:316266/1‑148 (MQ=255)
                                                                                             ggACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGt                                                   >  2:2492879/1‑148 (MQ=255)
                                                                                              gACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGtt                                                  >  2:3311671/1‑148 (MQ=255)
                                                                                                 gCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTa                                               <  2:2039406/148‑1 (MQ=255)
                                                                                                   aCGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACg                                             <  1:2885903/148‑1 (MQ=255)
                                                                                                    cGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGc                                            >  2:2218087/1‑148 (MQ=255)
                                                                                                    cGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGc                                            <  1:3204445/148‑1 (MQ=255)
                                                                                                      aTAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCtt                                          <  2:1407161/148‑1 (MQ=255)
                                                                                                        agagagTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTg                                        <  2:57001/148‑1 (MQ=255)
                                                                                                            agTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACAGAAGTCAGGTTGTACGCTTTGTTcc                                    >  1:2302436/1‑148 (MQ=255)
                                                                                                             gTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCa                                   <  2:611863/148‑1 (MQ=255)
                                                                                                             gTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCa                                   <  1:1968884/148‑1 (MQ=255)
                                                                                                              tGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCAt                                  <  2:848836/148‑1 (MQ=255)
                                                                                                              tGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCAt                                  <  2:3378608/148‑1 (MQ=255)
                                                                                                                 aaTGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTa                               >  2:781534/1‑148 (MQ=255)
                                                                                                                   tGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATg                             <  1:822281/148‑1 (MQ=255)
                                                                                                                   tGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATg                             <  1:822410/148‑1 (MQ=255)
                                                                                                                    gCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATgc                            >  1:483917/1‑148 (MQ=255)
                                                                                                                    gCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATgc                            >  1:535794/1‑148 (MQ=255)
                                                                                                                       tGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATgcagc                         <  1:2002603/148‑1 (MQ=255)
                                                                                                                       tGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATgcagc                         >  1:1537303/1‑148 (MQ=255)
                                                                                                                         gCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCat                       >  2:2595749/1‑148 (MQ=255)
                                                                                                                             tCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTc                   >  2:409093/1‑148 (MQ=255)
                                                                                                                             tCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTc                   >  2:279415/1‑148 (MQ=255)
                                                                                                                             tCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTc                   >  2:265878/1‑148 (MQ=255)
                                                                                                                              ccTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCa                  <  1:600110/148‑1 (MQ=255)
                                                                                                                              ccTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCa                  <  1:215743/148‑1 (MQ=255)
                                                                                                                                 ggTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGt               <  2:2516431/148‑1 (MQ=255)
                                                                                                                                  gTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATGTATGCAGCATATTCACGTa              >  2:964489/1‑148 (MQ=255)
                                                                                                                                       gTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCaa         <  1:1311483/148‑1 (MQ=255)
                                                                                                                                             aCATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCt   >  2:296931/1‑148 (MQ=255)
                                                                                                                                             aCATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCt   <  1:217130/148‑1 (MQ=255)
                                                                                                                                             aCATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCt   <  2:2426146/148‑1 (MQ=255)
                                                                                                                                              cATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTg  >  1:3514008/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTG  >  CP009273/3916685‑3916974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑