Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,917,554 C→A 100% L480L (CTG→CTT)  mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,917,5540CA100.0% 535.4 / ‑6.7 171L480L (CTG→CTT) mnmG5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base A (79/89);  minor base T (1/1);  total (81/90)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 171 total aligned reads displayed.

CGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTGCG  >  CP009273/3917410‑3917698
                                                                                                                                                |                                                                                                                                                
cGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAga                                                                                                                                               >  2:861774/1‑148 (MQ=255)
   cGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgc                                                                                                                                            <  2:1967498/148‑1 (MQ=255)
   cGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgc                                                                                                                                            >  1:893703/1‑148 (MQ=255)
   cGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgc                                                                                                                                            <  1:577734/148‑1 (MQ=255)
     tCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctg                                                                                                                                          <  1:3485943/148‑1 (MQ=255)
     tCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctg                                                                                                                                          <  1:3353457/148‑1 (MQ=255)
      cAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctga                                                                                                                                         <  1:1101066/148‑1 (MQ=255)
      cAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctga                                                                                                                                         <  2:1030929/148‑1 (MQ=255)
      cAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctga                                                                                                                                         >  1:2566027/1‑148 (MQ=255)
      cAGCGTGGTTAATTTTTCATAAGTCAGTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctga                                                                                                                                         <  1:2445479/148‑1 (MQ=255)
         cGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACAGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAtaagctgacgc                                                                                                                                      >  1:2693766/1‑148 (MQ=255)
         cGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACAGGCTTCACGGGAAAGCGGCGCACTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCCACGGGGTTAACCAGGTCGATTTTAaacgcagaacc                                                                                                                                      >  1:2693979/1‑145 (MQ=255)
            ggTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctca                                                                                                                                   >  2:3361404/1‑148 (MQ=255)
            ggTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctca                                                                                                                                   >  2:2715716/1‑148 (MQ=255)
              ttAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctcacg                                                                                                                                 >  1:1330190/1‑148 (MQ=255)
               tAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctcacgc                                                                                                                                >  2:628803/1‑148 (MQ=255)
                aaTTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctcacgct                                                                                                                               <  2:3456523/148‑1 (MQ=255)
                 aTTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctcacgctc                                                                                                                              >  2:678788/1‑148 (MQ=255)
                  tttttCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCg                                                                                                                             <  1:2056698/148‑1 (MQ=255)
                   ttttCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGATGACGCTCACGCTCGa                                                                                                                            <  2:3361964/148‑1 (MQ=255)
                    tttCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGat                                                                                                                           <  1:1478806/148‑1 (MQ=255)
                    tttCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTAACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGat                                                                                                                           <  2:405203/148‑1 (MQ=255)
                     ttCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGata                                                                                                                          <  1:2715716/148‑1 (MQ=255)
                        aTAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATtc                                                                                                                       >  2:3361105/1‑148 (MQ=255)
                         tAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATtct                                                                                                                      <  2:3236797/148‑1 (MQ=255)
                         tAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATtct                                                                                                                      <  2:3395250/148‑1 (MQ=255)
                           aGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCa                                                                                                                    <  2:2605992/148‑1 (MQ=255)
                            gTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCaa                                                                                                                   >  1:2643344/1‑148 (MQ=255)
                            gTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCaa                                                                                                                   >  1:1757056/1‑148 (MQ=255)
                                 ttCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTtc                                                                                                              <  1:2946568/148‑1 (MQ=255)
                                      gacgacGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGtt                                                                                                         >  2:34015/1‑148 (MQ=255)
                                                 agATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGccc                                                                                              <  2:3439783/148‑1 (MQ=255)
                                                  gATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCa                                                                                             >  2:752246/1‑148 (MQ=255)
                                                  gATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCa                                                                                             >  2:2624085/1‑148 (MQ=255)
                                                   aTCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCaa                                                                                            >  1:2491903/1‑148 (MQ=255)
                                                    tCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAAc                                                                                           >  1:3093433/1‑148 (MQ=255)
                                                       tCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGtt                                                                                        >  2:2834924/1‑148 (MQ=255)
                                                                gCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATcca                                                                               >  1:1525047/1‑148 (MQ=255)
                                                                gCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATcca                                                                               >  2:1158087/1‑148 (MQ=255)
                                                                 cTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATccac                                                                              >  1:3474586/1‑148 (MQ=255)
                                                                 cTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGAGTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATccac                                                                              >  1:621623/1‑148 (MQ=255)
                                                                   tCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATccacca                                                                            <  1:2515695/148‑1 (MQ=255)
                                                                     aCGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAgg                                                                          >  2:475044/1‑148 (MQ=255)
                                                                        ggAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGccc                                                                       <  2:3492692/148‑1 (MQ=255)
                                                                        ggAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGccc                                                                       <  2:3492717/148‑1 (MQ=255)
                                                                        ggAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGccc                                                                       <  1:3303748/148‑1 (MQ=255)
                                                                        ggAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGccc                                                                       <  2:592080/148‑1 (MQ=255)
                                                                        ggAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGccc                                                                       <  1:3304045/148‑1 (MQ=255)
                                                                        ggAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGccc                                                                       <  2:201406/148‑1 (MQ=255)
                                                                        ggAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGccc                                                                       <  2:1970517/148‑1 (MQ=255)
                                                                          aaaGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAg                                                                     >  2:2163701/1‑148 (MQ=255)
                                                                                 cgcAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCGCCAGGCCCAGTTCACGa                                                              <  1:38361/148‑1 (MQ=255)
                                                                                   cAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGAcc                                                            >  2:930584/1‑148 (MQ=255)
                                                                                    aGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCg                                                           <  2:1190225/148‑1 (MQ=255)
                                                                                     gTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGa                                                          <  1:628803/148‑1 (MQ=255)
                                                                                     gTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGa                                                          <  1:2065263/148‑1 (MQ=255)
                                                                                      tCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGAt                                                         <  2:1634635/148‑1 (MQ=255)
                                                                                      tCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGAt                                                         <  1:3200083/148‑1 (MQ=255)
                                                                                      tCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTATCGTTAAAGCGCGCCCAACGGTCGTCATCCACCAGGCCCAGTTCACGACCGAt                                                         >  1:322775/1‑148 (MQ=255)
                                                                                      tCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCGCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTAGTCATACACCAGGCCCCGTTCACGACCGAt                                                         >  1:322679/1‑148 (MQ=255)
                                                                                       cAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGAtt                                                        >  2:2194415/1‑148 (MQ=255)
                                                                                       cAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGAtt                                                        >  2:2437968/1‑148 (MQ=255)
                                                                                        aGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGAttt                                                       <  1:1008943/148‑1 (MQ=255)
                                                                                         ggTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTc                                                      <  1:3497326/148‑1 (MQ=255)
                                                                                           tGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTTAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAg                                                    <  2:285347/148‑1 (MQ=255)
                                                                                           tGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAg                                                    <  2:3182084/148‑1 (MQ=255)
                                                                                             aGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTc                                                  >  1:2347703/1‑148 (MQ=255)
                                                                                                 ttCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAAc                                              >  2:3405521/1‑148 (MQ=255)
                                                                                                     cTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAg                                          >  1:809089/1‑148 (MQ=255)
                                                                                                     cTTCGGCTGCAGCGTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAg                                          <  2:2959013/148‑1 (MQ=255)
                                                                                                       tCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATACACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGAt                                        >  2:3373826/1‑148 (MQ=255)
                                                                                                          gCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCg                                     <  1:34067/148‑1 (MQ=255)
                                                                                                          gCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCg                                     <  1:34015/148‑1 (MQ=255)
                                                                                                          gCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCg                                     <  1:3441446/148‑1 (MQ=255)
                                                                                                             gCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCAt                                  <  1:1998450/148‑1 (MQ=255)
                                                                                                             gCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCAt                                  <  1:2907528/148‑1 (MQ=255)
                                                                                                             gCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCAt                                  >  2:52921/1‑148 (MQ=255)
                                                                                                              cAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCAtt                                 >  2:1759776/1‑148 (MQ=255)
                                                                                                              cAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATACGCAtt                                 >  2:2034457/1‑148 (MQ=255)
                                                                                                                gCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTAt                               <  2:1162982/148‑1 (MQ=255)
                                                                                                                 cTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATc                              >  2:1816164/1‑148 (MQ=255)
                                                                                                                 cTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATc                              <  2:746368/148‑1 (MQ=255)
                                                                                                                 cTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATc                              >  2:37436/1‑148 (MQ=255)
                                                                                                                  ttCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCCGATCCGCATTATCt                             >  2:427480/1‑148 (MQ=255)
                                                                                                                   tCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCtt                            <  1:3207627/148‑1 (MQ=255)
                                                                                                                   tCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCtt                            >  2:2835003/1‑148 (MQ=255)
                                                                                                                   tCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCtt                            <  2:2369155/148‑1 (MQ=255)
                                                                                                                     cgccgACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTcg                          <  2:1060790/148‑1 (MQ=255)
                                                                                                                       ccgACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTcgcg                        <  1:2002234/148‑1 (MQ=255)
                                                                                                                             gggTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCAt                  <  2:3031924/148‑1 (MQ=255)
                                                                                                                                ttACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAg               <  1:861774/148‑1 (MQ=255)
                                                                                                                                 tACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGa              <  2:152763/148‑1 (MQ=255)
                                                                                                                                       ggTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGatat        <  1:2623948/148‑1 (MQ=255)
                                                                                                                                       ggTCGATTTAAGACGCTGACGCTCACGCTCGATAGTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGatat        <  2:2670154/148‑1 (MQ=255)
                                                                                                                                        gTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATAtt       <  2:1129036/148‑1 (MQ=255)
                                                                                                                                        gTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATAtt       >  2:308248/1‑148 (MQ=255)
                                                                                                                                         tCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTc      <  1:437113/148‑1 (MQ=255)
                                                                                                                                          cGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCt     <  1:968184/148‑1 (MQ=255)
                                                                                                                                           gATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTg    <  2:1276295/148‑1 (MQ=255)
                                                                                                                                             tttAAGACGCTGACGCTCACGCTCGATAGTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCAAGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTgcg  <  2:3311134/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTGCG  >  CP009273/3917410‑3917698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑