Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,935,100 G→T 100% intergenic (‑413/‑68) yieP ← / → rrsC putative transcriptional regulator/16S ribosomal RNA of rrnC operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,935,1000GT97.0% 217.6 / 2.0 101intergenic (‑413/‑68)yieP/rrsCputative transcriptional regulator/16S ribosomal RNA of rrnC operon
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base T (43/55);  total (43/58)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 101 total aligned reads displayed.

ATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCA  >  CP009273/3934955‑3935160
                                                                                                                                                 |                                                            
atGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTaa                                                            >  1:3420579/1‑148 (MQ=21)
 tGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAAt                                                           <  2:2581773/148‑1 (MQ=21)
 tGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAAt                                                           <  2:26580/148‑1 (MQ=21)
 tGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAAt                                                           <  2:648058/148‑1 (MQ=21)
  gCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATa                                                          >  2:379778/1‑148 (MQ=21)
     tGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCa                                                       >  2:1064097/1‑148 (MQ=21)
     tGACTCTGTAGCGAGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCa                                                       >  2:3254554/1‑148 (MQ=21)
       aCTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAg                                                     >  1:2993953/1‑148 (MQ=21)
       aCTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAg                                                     <  2:2831167/148‑1 (MQ=21)
        ctctGTAGCGGGAAAGGTTTTTATGCCCACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGt                                                    <  1:721639/148‑1 (MQ=11)
        ctctGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGt                                                    <  1:273470/148‑1 (MQ=21)
        ctctGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGt                                                    <  1:1483900/148‑1 (MQ=21)
        ctctGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGt                                                    <  2:1075800/148‑1 (MQ=21)
         tctGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGtc                                                   >  1:3443844/1‑148 (MQ=21)
         tctGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGtc                                                   <  2:706960/148‑1 (MQ=21)
          ctGTAGCGGTAAAGCGTATTATGCACACCCCGCGCCGCTTATAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGtct                                                  <  1:2407904/148‑1 (MQ=255)
           tGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGtctc                                                 <  2:1771730/148‑1 (MQ=21)
             tAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCaa                                               <  2:271472/148‑1 (MQ=21)
              aGCGGGAAAGCTTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAag                                              <  2:49928/148‑1 (MQ=21)
              aGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAag                                              <  2:292456/148‑1 (MQ=21)
              aGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAag                                              <  1:584122/148‑1 (MQ=21)
                 gttaaaGCTTATTATCCACACCCCGCGCGTCTGATAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGt                                           <  1:768959/145‑1 (MQ=11)
                 ggtaaaGCGTATTAGGCACACCCCGCGCCGCTGATAAAAAGAAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGt                                           <  1:620695/145‑1 (MQ=11)
                 gggAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGt                                           <  2:1723373/148‑1 (MQ=21)
                  ggAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTg                                          >  1:2987995/1‑148 (MQ=21)
                   taaaGCGTATTATGCACACCCCGCGCCGATGATAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGTGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGa                                         <  2:138826/147‑1 (MQ=255)
                   gAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGa                                         <  2:1505440/148‑1 (MQ=21)
                   gAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGa                                         >  2:1237141/1‑148 (MQ=21)
                   gAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGa                                         <  1:1518550/148‑1 (MQ=21)
                   gAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGa                                         >  2:3376283/1‑148 (MQ=21)
                   gAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGa                                         >  1:2487563/1‑148 (MQ=21)
                   gAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTATCAAGAGTGa                                         <  1:3144392/148‑1 (MQ=21)
                    aaaGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGaa                                        >  1:1050873/1‑148 (MQ=21)
                      aGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAaca                                      >  1:1249620/1‑148 (MQ=17)
                      aGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAaca                                      >  1:491990/1‑148 (MQ=17)
                       gCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAacac                                     >  1:1054032/1‑148 (MQ=17)
                       gCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAacac                                     <  2:529106/148‑1 (MQ=17)
                       gCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAacac                                     >  2:1978605/1‑148 (MQ=17)
                       gCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAacac                                     >  2:1978610/1‑148 (MQ=17)
                       gCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAacac                                     >  1:3027687/1‑148 (MQ=17)
                       gCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAacac                                     >  1:1730990/1‑148 (MQ=17)
                        cGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACg                                    >  1:1748043/1‑148 (MQ=17)
                        cGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACg                                    >  1:1743685/1‑148 (MQ=17)
                        cGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACg                                    >  1:1743702/1‑148 (MQ=17)
                        cGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAAAACg                                    >  2:2241608/1‑148 (MQ=17)
                        cGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATAAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACg                                    >  1:362913/1‑148 (MQ=17)
                         gTATTATGCACACCCCGCGCCGCTTATAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGt                                   <  1:1927962/148‑1 (MQ=12)
                          tattatGCACACCCCGCGCCGCTGATAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTa                                  <  1:147602/148‑1 (MQ=17)
                          tattatGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTa                                  <  1:1922339/148‑1 (MQ=17)
                          tattatGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTa                                  >  1:3262726/1‑148 (MQ=17)
                          tattatGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTa                                  >  2:2604998/1‑148 (MQ=17)
                           attatGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTaa                                 <  1:77392/148‑1 (MQ=17)
                           attatGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTaa                                 >  1:74297/1‑148 (MQ=17)
                           attatGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTaa                                 >  1:1853236/1‑148 (MQ=17)
                            ttatGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAAt                                <  2:2712163/148‑1 (MQ=17)
                             tatGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAAtt                               >  1:1571838/1‑148 (MQ=17)
                             tatGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAAtt                               <  1:123521/148‑1 (MQ=17)
                               tGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCa                             <  2:3066974/148‑1 (MQ=17)
                               tGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCa                             <  2:1146243/148‑1 (MQ=17)
                                gCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCAt                            <  2:156389/148‑1 (MQ=17)
                                  acacCCCGCGCCGATTATAAAAAGAAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTa                          <  1:419348/148‑1 (MQ=11)
                                   cacCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTAc                         >  1:2060309/1‑148 (MQ=17)
                                   cacCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTAc                         >  1:2060297/1‑148 (MQ=17)
                                   cacCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTAc                         >  1:2059524/1‑148 (MQ=17)
                                    acCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACg                        <  1:1910734/148‑1 (MQ=17)
                                     ccccGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGa                       <  2:1181425/148‑1 (MQ=17)
                                     ccccGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGa                       <  2:2947413/148‑1 (MQ=17)
                                     ccccGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGa                       <  1:1247680/148‑1 (MQ=32)
                                     ccccGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGa                       <  1:241266/148‑1 (MQ=32)
                                     ccccGCGCCGCGTACAAAAACCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGa                       <  1:2997570/148‑1 (MQ=255)
                                      cccGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGaa                      >  2:326536/1‑148 (MQ=17)
                                      cccGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGaa                      <  1:1778579/148‑1 (MQ=17)
                                      cccGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGaa                      <  1:1777744/148‑1 (MQ=17)
                                      cccGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGaa                      >  2:325397/1‑148 (MQ=17)
                                       ccGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAg                     <  2:444131/148‑1 (MQ=17)
                                       ccGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAg                     <  2:1620683/148‑1 (MQ=17)
                                       ccGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAg                     <  1:2475772/148‑1 (MQ=17)
                                        cgcgCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGt                    <  2:1530173/148‑1 (MQ=17)
                                        cgcgCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGt                    <  2:1530168/148‑1 (MQ=17)
                                        cgcgCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGt                    <  2:1980161/148‑1 (MQ=17)
                                         gcgcCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGtt                   <  2:3204900/148‑1 (MQ=17)
                                            ccgcTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTaa                >  1:1089395/1‑148 (MQ=17)
                                            ccgcTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTaa                >  1:1090988/1‑148 (MQ=17)
                                            ccgcTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTaa                <  2:1487733/148‑1 (MQ=17)
                                             gtttGATAAAATGCAACGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAAt               <  1:1266843/145‑1 (MQ=11)
                                             cgcTGAGAAAAAGCAAAGCGTCACTGCTCTTTAACAATTTATCAGACGATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAAt               <  2:2119792/148‑1 (MQ=12)
                                             cgcTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAAt               <  2:403767/148‑1 (MQ=17)
                                              gcTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAAtt              <  2:462139/148‑1 (MQ=17)
                                                tGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCt            >  1:1071731/1‑148 (MQ=17)
                                                tGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCt            >  1:2823572/1‑148 (MQ=17)
                                                    aaaaaGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGa        <  1:454051/148‑1 (MQ=17)
                                                      aaagcaaagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGc      >  1:2979340/1‑148 (MQ=17)
                                                      aaagcaaagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGc      >  2:1019790/1‑148 (MQ=17)
                                                      aaagcaaagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGc      <  2:2984922/148‑1 (MQ=17)
                                                      aaagcaaagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGc      >  2:1019786/1‑148 (MQ=17)
                                                      aaagcaaagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGc      >  2:1019784/1‑148 (MQ=17)
                                                        agcaaagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCAt    <  1:76262/148‑1 (MQ=17)
                                                        agcaaagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCAt    <  1:76315/148‑1 (MQ=17)
                                                          caaagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCa  >  2:2591160/1‑148 (MQ=14)
                                                          caaagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCa  >  2:101319/1‑148 (MQ=14)
                                                                                                                                                 |                                                            
ATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCA  >  CP009273/3934955‑3935160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑