Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,959,351 G→T 100% P77P (CCG→CCT)  trxA → thioredoxin 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,959,3510GT98.8% 850.8 / 0.4 260P77P (CCG→CCT) trxAthioredoxin 1
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (150/107);  total (152/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 260 total aligned reads displayed.

GGGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGCCC  >  CP009273/3959206‑3959495
                                                                                                                                                 |                                                                                                                                                
gggCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGCCACTGCGCCGAAATATGGCATCCGTGGTATCCCTAc                                                                                                                                                >  1:1810910/1‑148 (MQ=255)
      agTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctgct                                                                                                                                          >  2:2717301/1‑148 (MQ=255)
        tggtgCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctgctgc                                                                                                                                        <  2:2772360/148‑1 (MQ=255)
          gtgCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctgctgctg                                                                                                                                      <  1:2559532/148‑1 (MQ=255)
           tgCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGt                                                                                                                                     <  1:1059741/148‑1 (MQ=255)
           tgCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGt                                                                                                                                     <  1:3252675/148‑1 (MQ=255)
                tCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCaaa                                                                                                                                <  1:415194/148‑1 (MQ=255)
                tCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCaaa                                                                                                                                >  1:1374608/1‑148 (MQ=255)
                 ccGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCaaaa                                                                                                                               <  2:1650247/148‑1 (MQ=255)
                 ccGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCaaaa                                                                                                                               <  2:1480420/148‑1 (MQ=255)
                    tGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACg                                                                                                                            <  1:1446705/148‑1 (MQ=255)
                    tGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACg                                                                                                                            >  2:2169288/1‑148 (MQ=255)
                     gCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACgg                                                                                                                           >  2:1151521/1‑148 (MQ=255)
                     gCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACgg                                                                                                                           <  1:2276246/148‑1 (MQ=255)
                     gCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACgg                                                                                                                           >  2:3178675/1‑148 (MQ=255)
                     gCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACgg                                                                                                                           >  2:2902635/1‑148 (MQ=255)
                         aaTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGaa                                                                                                                       >  1:1684453/1‑148 (MQ=255)
                         aaTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGaa                                                                                                                       <  1:3412055/148‑1 (MQ=255)
                          aTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAg                                                                                                                      >  1:2682288/1‑148 (MQ=255)
                          aTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAg                                                                                                                      >  1:2607946/1‑148 (MQ=255)
                           tGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGt                                                                                                                     >  2:3396865/1‑148 (MQ=255)
                             aTCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTgg                                                                                                                   <  2:1852712/148‑1 (MQ=255)
                               cGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTggcg                                                                                                                 >  1:270413/1‑148 (MQ=255)
                               cGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTggcg                                                                                                                 <  1:2468341/148‑1 (MQ=255)
                                gCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTggcgg                                                                                                                <  1:564601/148‑1 (MQ=255)
                                 ccccGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTggcggc                                                                                                               >  2:86467/1‑148 (MQ=255)
                                  cccGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCa                                                                                                              >  2:1075167/1‑148 (MQ=255)
                                  cccGATTCTGGAAGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCa                                                                                                              <  2:1340490/148‑1 (MQ=255)
                                   ccGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCaa                                                                                                             >  2:1687724/1‑148 (MQ=255)
                                   ccGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCaa                                                                                                             <  2:2663420/148‑1 (MQ=255)
                                    cGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAAc                                                                                                            <  2:1372483/148‑1 (MQ=255)
                                    cGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAAc                                                                                                            >  1:356629/1‑148 (MQ=255)
                                     gATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAAcc                                                                                                           >  1:1037901/1‑148 (MQ=255)
                                       ttCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCaa                                                                                                         >  2:1163718/1‑148 (MQ=255)
                                       ttCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCaa                                                                                                         <  2:151621/148‑1 (MQ=255)
                                       ttCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCaa                                                                                                         <  1:2239682/148‑1 (MQ=255)
                                        tCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCaaa                                                                                                        >  1:2878879/1‑148 (MQ=255)
                                              tGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGt                                                                                                  >  2:345845/1‑148 (MQ=255)
                                               gAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTg                                                                                                 <  2:354272/148‑1 (MQ=255)
                                                    cGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTCCAATAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTg                                                                                            <  1:1337074/148‑1 (MQ=255)
                                                         aCGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTaa                                                                                       <  1:1159158/148‑1 (MQ=255)
                                                           gAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAg                                                                                     <  1:1825849/148‑1 (MQ=255)
                                                               atCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCa                                                                                 >  1:2961851/1‑148 (MQ=255)
                                                               atCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCa                                                                                 <  1:2335035/148‑1 (MQ=255)
                                                                tCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAg                                                                                >  2:2382921/1‑148 (MQ=255)
                                                                tCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGCCTAAAGGTCAg                                                                                >  1:1787891/1‑148 (MQ=255)
                                                                 cAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGt                                                                               <  1:1222596/148‑1 (MQ=255)
                                                                  aGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGtt                                                                              >  2:3049075/1‑148 (MQ=255)
                                                                  aGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGtt                                                                              >  2:360991/1‑148 (MQ=255)
                                                                   gggCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTg                                                                             >  2:1963638/1‑148 (MQ=255)
                                                                    ggCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGa                                                                            >  1:1615391/1‑148 (MQ=255)
                                                                     gCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGaa                                                                           >  2:3194553/1‑148 (MQ=255)
                                                                      cAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGaaa                                                                          >  1:1639600/1‑148 (MQ=255)
                                                                      cAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGaaa                                                                          >  2:2229961/1‑148 (MQ=255)
                                                                         aCTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTTGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAagag                                                                       <  1:2169288/148‑1 (MQ=255)
                                                                         aCTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAagag                                                                       <  1:1262151/148‑1 (MQ=255)
                                                                             aCCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTcc                                                                   <  2:1078317/148‑1 (MQ=255)
                                                                             aCCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTcc                                                                   <  2:3017255/148‑1 (MQ=255)
                                                                               cGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTc                                                                 >  2:1339092/1‑148 (MQ=255)
                                                                               cGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTc                                                                 >  2:2094650/1‑148 (MQ=255)
                                                                               cGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGGTGAAAGAGTTCCTc                                                                 >  2:363971/1‑148 (MQ=255)
                                                                                 ttGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGa                                                               <  1:2459735/148‑1 (MQ=255)
                                                                                 ttGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGa                                                               >  1:3473240/1‑148 (MQ=255)
                                                                                  tGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGAc                                                              >  1:491433/1‑148 (MQ=255)
                                                                                   gCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACg                                                             <  1:923557/148‑1 (MQ=255)
                                                                                    cAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGc                                                            >  2:924526/1‑148 (MQ=255)
                                                                                    cAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGc                                                            <  1:153647/148‑1 (MQ=255)
                                                                                        aCTGAACATCGATCAAAACCCTGTCACTGCGCCGAAATATGTAATCCTTGTTATACCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCTGCACCCAAAGTGGTTGCACTGTAAAAAGGTCAGTTTAAAGAGTTCATCGACGATAAc                                                        >  2:2753932/1‑148 (MQ=255)
                                                                                          tGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCt                                                      <  2:2339723/148‑1 (MQ=255)
                                                                                            aaCATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTgg                                                    >  1:2042145/1‑148 (MQ=255)
                                                                                              cATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCg                                                  >  2:410011/1‑148 (MQ=255)
                                                                                                tCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTa                                                >  2:601461/1‑148 (MQ=255)
                                                                                                 cGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTaa                                               >  2:1749031/1‑148 (MQ=255)
                                                                                                 cGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTaa                                               >  1:1460298/1‑148 (MQ=255)
                                                                                                 cGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTaa                                               >  2:2885963/1‑148 (MQ=255)
                                                                                                  gATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAg                                              <  2:483367/148‑1 (MQ=255)
                                                                                                  gATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAg                                              <  1:2395132/148‑1 (MQ=255)
                                                                                                   aTCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAgg                                             <  2:1442594/148‑1 (MQ=255)
                                                                                                    tCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAggg                                            >  2:894619/1‑148 (MQ=255)
                                                                                                     cAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGa                                           >  1:231527/1‑148 (MQ=255)
                                                                                                       aaaCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAt                                         >  2:2838307/1‑148 (MQ=255)
                                                                                                        aaCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAtt                                        >  2:763110/1‑148 (MQ=255)
                                                                                                        aaCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAtt                                        >  1:3226105/1‑148 (MQ=255)
                                                                                                         aCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAttt                                       >  2:2715429/1‑148 (MQ=255)
                                                                                                           ccTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAtttga                                     <  2:1780993/148‑3 (MQ=255)
                                                                                                           ccTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGAGTAAGGGAATTTCa                                     <  1:3357006/148‑1 (MQ=255)
                                                                                                             tGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATg                                   >  2:886197/1‑148 (MQ=255)
                                                                                                             tGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATg                                   >  2:2205998/1‑148 (MQ=255)
                                                                                                               gCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGtt                                 >  2:3298408/1‑148 (MQ=255)
                                                                                                               gCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGtt                                 >  1:154044/1‑148 (MQ=255)
                                                                                                                               tGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAGTTCATGTTCGGGTGCCCCGTCGCt                 >  2:2192650/1‑148 (MQ=255)
                                                                                                                                 gCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTaa               >  2:722062/1‑148 (MQ=255)
                                                                                                                                    tCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTaaaaa            >  1:702668/1‑148 (MQ=255)
                                                                                                                                    tCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTaaaaa            >  2:700265/1‑148 (MQ=255)
                                                                                                                                     ccGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTaaaaaa           <  2:3127938/148‑2 (MQ=255)
                                                                                                                                         ggTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGACTTTCATGTTCGGGTGCCCCGTCGCTCTAAACTGGa       >  2:3225979/1‑148 (MQ=255)
                                                                                                                                         ggTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGa       <  2:2485566/148‑1 (MQ=255)
                                                                                                                                           tATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACg     >  1:3467099/1‑148 (MQ=255)
                                                                                                                                             tCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGcc   <  2:3068039/148‑1 (MQ=255)
                                                                                                                                              cccTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGccc  >  1:2057375/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGCCC  >  CP009273/3959206‑3959495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑