Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 300,770 C→A 100% V49V (GTG→GTT)  ecpE ← ECP production pilus chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273300,7700CA98.6% 702.3 / 0.8 216V49V (GTG→GTT) ecpEECP production pilus chaperone
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (106/107);  total (108/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 216 total aligned reads displayed.

ACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAAC  >  CP009273/300625‑300914
                                                                                                                                                 |                                                                                                                                                
aCTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAAc                                                                                                                                                >  1:3489585/1‑148 (MQ=255)
aCTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAAc                                                                                                                                                >  1:1378155/1‑148 (MQ=255)
aCTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAAc                                                                                                                                                >  1:3489761/1‑148 (MQ=255)
aCTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAAc                                                                                                                                                >  2:2795144/1‑148 (MQ=255)
aCTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAAc                                                                                                                                                <  2:2027071/148‑1 (MQ=255)
 cTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACt                                                                                                                                               >  1:850003/1‑148 (MQ=255)
 cTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACt                                                                                                                                               <  2:760090/148‑1 (MQ=255)
 cTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAAGAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACt                                                                                                                                               <  2:759056/148‑1 (MQ=255)
    gCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTaca                                                                                                                                            <  1:3244545/148‑1 (MQ=255)
    gCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTaca                                                                                                                                            <  1:1722733/148‑1 (MQ=255)
      tctcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACg                                                                                                                                          >  2:2525435/1‑148 (MQ=255)
       ctcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGt                                                                                                                                         <  1:838272/148‑1 (MQ=255)
       ctcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGt                                                                                                                                         >  1:487139/1‑148 (MQ=255)
        tcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGtt                                                                                                                                        <  2:3140439/148‑1 (MQ=255)
        tcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGtt                                                                                                                                        >  1:1565588/1‑148 (MQ=255)
        tcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCGATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGtt                                                                                                                                        <  1:385215/148‑1 (MQ=255)
         cACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGttt                                                                                                                                       >  2:3313908/1‑148 (MQ=255)
          aCCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTg                                                                                                                                      >  1:322016/1‑148 (MQ=255)
           ccAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGc                                                                                                                                     >  2:881510/1‑148 (MQ=255)
              gCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGa                                                                                                                                  <  2:2981506/148‑1 (MQ=255)
               ccTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCACTACGGTCTATCCGTGCGCTTGTGTTGTTATTAACTACACGTTTGCTGAc                                                                                                                                 >  2:3365601/1‑148 (MQ=255)
               ccTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGAc                                                                                                                                 >  2:160300/1‑148 (MQ=255)
                cTGCACCGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACa                                                                                                                                <  2:832071/148‑1 (MQ=255)
                cTGCAACGCCAGCTGGCGGGGGGCGAAAAGCTGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACa                                                                                                                                >  1:3025852/1‑148 (MQ=255)
                cTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACa                                                                                                                                <  2:832106/148‑1 (MQ=255)
                   cAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAag                                                                                                                             >  2:2882874/1‑148 (MQ=255)
                      cGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtca                                                                                                                          <  2:2729685/148‑1 (MQ=255)
                         cAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtcagtc                                                                                                                       <  2:2564328/148‑1 (MQ=255)
                         cAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtcagtc                                                                                                                       <  2:3266694/148‑1 (MQ=255)
                            cTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCg                                                                                                                    >  1:3296636/1‑148 (MQ=255)
                            cTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCg                                                                                                                    >  1:1953072/1‑148 (MQ=255)
                             tGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCgg                                                                                                                   <  2:1936680/148‑1 (MQ=255)
                             tGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCgg                                                                                                                   <  1:1807131/148‑1 (MQ=255)
                              ggCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGa                                                                                                                  <  1:240486/148‑1 (MQ=255)
                              ggCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGa                                                                                                                  <  2:3261434/148‑1 (MQ=255)
                               gCGGGGGGCGAAAAGCAGTTCACCATCCTCCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGAc                                                                                                                 <  2:3470500/148‑1 (MQ=255)
                               gCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACTCGTTTGCTGACAAAGTCAGTCTCGGAc                                                                                                                 >  2:2155317/1‑148 (MQ=255)
                               gCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGAc                                                                                                                 >  2:2232967/1‑148 (MQ=255)
                               gCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGAc                                                                                                                 >  2:2233006/1‑148 (MQ=255)
                                         aaaaGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGaaa                                                                                                       <  1:2795030/148‑1 (MQ=255)
                                         aaaaGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGaaa                                                                                                       <  2:689713/148‑1 (MQ=255)
                                          aaaGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGaaaa                                                                                                      >  1:1205039/1‑148 (MQ=255)
                                          aaaGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGaaaa                                                                                                      >  1:1205320/1‑148 (MQ=255)
                                           aaGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAAt                                                                                                     <  1:910938/148‑1 (MQ=255)
                                            aGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATg                                                                                                    >  1:25390/1‑148 (MQ=255)
                                            aGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATg                                                                                                    >  2:243881/1‑148 (MQ=255)
                                                  tCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGAt                                                                                              >  1:951654/1‑148 (MQ=255)
                                                     ccATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGc                                                                                           <  1:3149017/148‑1 (MQ=255)
                                                       aTCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccg                                                                                         >  1:936565/1‑148 (MQ=255)
                                                       aTCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccg                                                                                         >  2:449169/1‑148 (MQ=255)
                                                        tCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccga                                                                                        >  2:2446629/1‑148 (MQ=255)
                                                         ccACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccgac                                                                                       <  1:1672953/148‑1 (MQ=255)
                                                                  gggTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGc                                                                              <  1:2804651/148‑1 (MQ=255)
                                                                   ggTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAACATGTCAGATTGCCGACCGAAATCGcc                                                                             >  2:2387873/1‑148 (MQ=255)
                                                                       cgcAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCtgtg                                                                         <  2:2826941/148‑1 (MQ=255)
                                                                           aTTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCaa                                                                     >  1:2091446/1‑148 (MQ=255)
                                                                            ttCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCaaa                                                                    <  2:931295/148‑1 (MQ=255)
                                                                             tCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAg                                                                   >  1:3451255/1‑148 (MQ=255)
                                                                              cACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAgg                                                                  <  1:3154945/148‑1 (MQ=255)
                                                                              cACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAgg                                                                  <  2:1710701/148‑1 (MQ=255)
                                                                               aCTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGt                                                                 <  2:1205320/148‑1 (MQ=255)
                                                                               aCTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGt                                                                 <  2:1205039/148‑1 (MQ=255)
                                                                                  gctgcCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTgcc                                                              >  2:2875251/1‑148 (MQ=255)
                                                                                    tgcCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTgccgc                                                            <  2:2999753/148‑1 (MQ=255)
                                                                                      cccGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCa                                                          >  1:3011738/1‑148 (MQ=255)
                                                                                          ggCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCAt                                                      <  1:330882/148‑1 (MQ=255)
                                                                                            cTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATAc                                                    >  1:1630425/1‑148 (MQ=255)
                                                                                                cAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGAc                                                >  2:3171438/1‑148 (MQ=255)
                                                                                                cAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGAc                                                >  2:1963397/1‑148 (MQ=255)
                                                                                                cAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGAc                                                >  1:1461908/1‑148 (MQ=255)
                                                                                                cAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGAc                                                <  1:2191717/148‑1 (MQ=255)
                                                                                                  aTAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCg                                              >  2:1576358/1‑148 (MQ=255)
                                                                                                    aGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCCGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCt                                            >  1:1122873/1‑148 (MQ=255)
                                                                                                     gCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTg                                           >  2:1818288/1‑148 (MQ=255)
                                                                                                      cACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGt                                          >  1:386452/1‑148 (MQ=255)
                                                                                                      cACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGt                                          <  1:62154/148‑1 (MQ=255)
                                                                                                      cACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGt                                          <  2:2873742/148‑1 (MQ=255)
                                                                                                      cACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGt                                          <  2:3056794/148‑1 (MQ=255)
                                                                                                       aCTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTc                                         <  2:784420/148‑1 (MQ=255)
                                                                                                       aCTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTc                                         <  2:2559998/148‑1 (MQ=255)
                                                                                                         tGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcag                                       <  1:1031636/148‑1 (MQ=255)
                                                                                                           aTGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagca                                     <  2:1999318/148‑1 (MQ=255)
                                                                                                            tGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagcag                                    <  2:573646/148‑1 (MQ=255)
                                                                                                             ggCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAgg                                   >  1:1050102/1‑148 (MQ=255)
                                                                                                                aagtcGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCTGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGcc                                <  2:3182217/144‑1 (MQ=255)
                                                                                                                aaTACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGcc                                >  1:536896/1‑148 (MQ=255)
                                                                                                                  tACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCtt                              >  1:2913495/1‑148 (MQ=255)
                                                                                                                   aCGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTa                             >  1:2045340/1‑148 (MQ=255)
                                                                                                                        atatCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTaa                        >  2:1428947/1‑148 (MQ=255)
                                                                                                                        atatCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTaa                        <  1:160108/148‑1 (MQ=255)
                                                                                                                           tCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGt                     <  2:487139/148‑1 (MQ=255)
                                                                                                                            ccGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGtt                    >  2:721950/1‑148 (MQ=255)
                                                                                                                             cGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTa                   <  2:920193/148‑1 (MQ=255)
                                                                                                                                gcgcTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGc                <  2:1696999/148‑1 (MQ=255)
                                                                                                                                gcgcTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGc                <  1:881510/148‑1 (MQ=255)
                                                                                                                                gcgcTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGc                <  1:3085062/148‑1 (MQ=255)
                                                                                                                                    ttttGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACg            >  1:593058/1‑148 (MQ=255)
                                                                                                                                             tATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGaa   >  2:3103012/1‑148 (MQ=255)
                                                                                                                                             tATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGaa   >  2:1007103/1‑148 (MQ=255)
                                                                                                                                              aTTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAAc  <  1:285706/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAAC  >  CP009273/300625‑300914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑