BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,969,710	G→T	100%	G25C (GGT→TGT)	wzxE →	O‑antigen translocase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,969,710	0	G	T	100.0%	786.4 / NA	236	G25C (GGT→TGT)	wzxE	O‑antigen translocase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (126/110); total (126/110)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 236 total aligned reads displayed.

CTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATC  >  CP009273/3969568‑3969854

cTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAg                                                                                                                                             <  1:1962017/148‑1 (MQ=255)

    tCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGc                                                                                                                                         >  2:2300865/1‑148 (MQ=255)

     cTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTTCTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCt                                                                                                                                        <  2:1515640/148‑1 (MQ=255)

     cTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCt                                                                                                                                        <  2:3108888/148‑1 (MQ=255)

      tACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTg                                                                                                                                       <  2:406174/148‑1 (MQ=255)

           ccTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGgt                                                                                                                                  >  1:3078496/1‑148 (MQ=255)

            cTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGgtg                                                                                                                                 >  1:2688972/1‑148 (MQ=255)

            cTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGgtg                                                                                                                                 <  2:3070699/148‑1 (MQ=255)

              gTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTc                                                                                                                               <  2:3208484/148‑1 (MQ=255)

                cGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCAt                                                                                                                             <  2:76185/148‑1 (MQ=255)

                    cGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTgg                                                                                                                         <  2:1390079/148‑1 (MQ=255)

                     gTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGt                                                                                                                        <  2:2718239/148‑1 (MQ=255)

                     gTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGt                                                                                                                        >  1:832666/1‑148 (MQ=255)

                      tCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTc                                                                                                                       <  2:753254/148‑1 (MQ=255)

                      tCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTc                                                                                                                       <  2:2464219/148‑1 (MQ=255)

                         aTCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCcgg                                                                                                                    >  2:2677989/1‑148 (MQ=255)

                          tCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCcggc                                                                                                                   >  2:1853380/1‑148 (MQ=255)

                          tCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCcggc                                                                                                                   >  1:3464998/1‑148 (MQ=255)

                           cAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCcggcg                                                                                                                  <  2:1296848/148‑1 (MQ=255)

                               gTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGc                                                                                                              <  2:1029232/148‑1 (MQ=255)

                                tACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCt                                                                                                             >  2:905872/1‑148 (MQ=255)

                                    gTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTggg                                                                                                         >  1:2753970/1‑148 (MQ=255)

                                     tAATTGCGACTTTGTTGAACTAATTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGc                                                                                                        <  2:1399552/148‑1 (MQ=255)

                                         tGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTggc                                                                                                    >  1:2311190/1‑148 (MQ=255)

                                         tGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTggc                                                                                                    >  1:553137/1‑148 (MQ=255)

                                           cGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTggcgg                                                                                                  >  2:705231/1‑148 (MQ=255)

                                             aCTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCa                                                                                                >  1:106757/1‑148 (MQ=255)

                                             aCTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCAGTTGGTCCGGCGGGGCGGGGGCTGGCGGCa                                                                                                >  2:1887246/1‑148 (MQ=255)

                                                ttgttgAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAAt                                                                                             <  1:930417/148‑1 (MQ=255)

                                                       actactTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGcc                                                                                      >  1:1948921/1‑148 (MQ=255)

                                                       actactTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGcc                                                                                      <  2:2131693/148‑1 (MQ=255)

                                                        ctactTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCa                                                                                     >  2:2335069/1‑148 (MQ=255)

                                                         tactTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAg                                                                                    >  2:1781938/1‑148 (MQ=255)

                                                          actTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGt                                                                                   <  1:2957134/148‑1 (MQ=255)

                                                           ctTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGtt                                                                                  >  1:2907768/1‑148 (MQ=255)

                                                            ttttCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTg                                                                                 >  2:2152881/1‑148 (MQ=255)

                                                            ttttCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTg                                                                                 <  1:45352/148‑1 (MQ=255)

                                                            ttttCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGACAGTTg                                                                                 <  1:3237742/148‑1 (MQ=255)

                                                             tttCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGa                                                                                >  1:2418285/1‑148 (MQ=255)

                                                              ttCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAgatgat                                                                               >  1:638930/1‑148 (MQ=255)

                                                              ttCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGAt                                                                               >  2:1168923/1‑148 (MQ=255)

                                                                ccTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTa                                                                             <  1:2082290/148‑1 (MQ=255)

                                                                ccTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTa                                                                             <  1:3206380/148‑1 (MQ=255)

                                                                  tGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTAcc                                                                           >  1:1023481/1‑148 (MQ=255)

                                                                    atatGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCCGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGt                                                                         >  2:541721/1‑148 (MQ=255)

                                                                    atatGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGt                                                                         >  1:3200737/1‑148 (MQ=255)

                                                                      atGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGc                                                                       >  2:1247504/1‑148 (MQ=255)

                                                                      atGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGc                                                                       <  1:3486820/148‑1 (MQ=255)

                                                                       tGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCt                                                                      <  1:2676575/148‑1 (MQ=255)

                                                                          cGTTGGCAAAAGCGTGCTTGTGGACGGCGGCCAGTACACTGGTCTAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGTCGGGGCTTGGGCTTGCGGCAAATTTCTGCCAGTTGATTACCGTGCTCgg                                                                   <  1:1631765/148‑1 (MQ=255)

                                                                            ttGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCg                                                                 >  1:771678/1‑148 (MQ=255)

                                                                            ttGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCg                                                                 <  2:2993777/148‑1 (MQ=255)

                                                                             tGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGt                                                                >  2:1227290/1‑148 (MQ=255)

                                                                               gCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGc                                                              <  1:527501/148‑1 (MQ=255)

                                                                               gCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGc                                                              <  1:2292813/148‑1 (MQ=255)

                                                                               gCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCGTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGc                                                              >  2:890446/1‑148 (MQ=255)

                                                                                 aaaaGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCtt                                                            >  1:2544368/1‑148 (MQ=255)

                                                                                  aaaGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTg                                                           >  2:454549/1‑148 (MQ=255)

                                                                                   aaGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGc                                                          >  1:735386/1‑148 (MQ=255)

                                                                                   aaGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGc                                                          <  1:847659/148‑1 (MQ=255)

                                                                                     gCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCg                                                        >  1:2029949/1‑148 (MQ=255)

                                                                                        tCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCgggg                                                     <  1:2070047/148‑1 (MQ=255)

                                                                                        tCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCgggg                                                     <  2:2501878/148‑1 (MQ=255)

                                                                                         ccTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGc                                                    >  2:1168725/1‑148 (MQ=255)

                                                                                         ccTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGc                                                    >  1:2002158/1‑148 (MQ=255)

                                                                                         ccTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGc                                                    >  1:1166460/1‑148 (MQ=255)

                                                                                          cTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCt                                                   <  2:1124351/148‑1 (MQ=255)

                                                                                          cTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCt                                                   <  2:2876455/148‑1 (MQ=255)

                                                                                          cTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCt                                                   <  1:1983342/148‑1 (MQ=255)

                                                                                          cTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCt                                                   >  1:2600081/1‑148 (MQ=255)

                                                                                           gtgtgGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTg                                                  <  2:3423140/147‑1 (MQ=255)

                                                                                            tgtgGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTgg                                                 >  1:1539455/1‑148 (MQ=255)

                                                                                            tgtgGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTgg                                                 >  1:2443101/1‑148 (MQ=255)

                                                                                               ggACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCAt                                              <  1:2959849/148‑1 (MQ=255)

                                                                                                gACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATc                                             <  2:3070272/148‑1 (MQ=255)

                                                                                                 aCGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCt                                            <  1:1398124/148‑1 (MQ=255)

                                                                                                 aCGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCt                                            <  2:3143321/148‑1 (MQ=255)

                                                                                                    gcggcCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTa                                         <  2:106757/148‑1 (MQ=255)

                                                                                                       gcCAGTACACTGGTCAAGATTGGTGTCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACg                                      <  1:3503264/148‑1 (MQ=255)

                                                                                                       gcCAGTACACTGGTCAAGATTGGTGTCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACg                                      <  1:3503575/148‑1 (MQ=255)

                                                                                                       gcCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACg                                      >  2:3479696/1‑148 (MQ=255)

                                                                                                         cAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGgt                                    <  2:3062600/148‑1 (MQ=255)

                                                                                                          aGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGgtg                                   <  2:3498070/148‑1 (MQ=255)

                                                                                                           gTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGgtgt                                  >  1:1674650/1‑148 (MQ=255)

                                                                                                              cacTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAAc                               <  2:2520589/148‑1 (MQ=255)

                                                                                                               acTGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAAcc                              >  2:2839878/1‑148 (MQ=255)

                                                                                                                 tGGTCAAGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCaa                            <  2:2586088/148‑1 (MQ=255)

                                                                                                                      aaGATTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACg                       >  2:1910509/1‑148 (MQ=255)

                                                                                                                         aTTGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTg                    <  2:573209/148‑1 (MQ=255)

                                                                                                                          ttGGTGCCGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGc                   >  1:2882818/1‑148 (MQ=255)

                                                                                                                           tGGTGCCGGGTTACTGGTCTGTAAGGTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGcc                  <  2:3357363/148‑1 (MQ=255)

                                                                                                                                ccGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTa             >  2:1723195/1‑148 (MQ=255)

                                                                                                                                ccGGGTTACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTa             <  1:1573699/148‑1 (MQ=255)

                                                                                                                                     ttACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATg        <  1:1088500/148‑1 (MQ=255)

                                                                                                                                      tACTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGa       >  1:170985/1‑148 (MQ=255)

                                                                                                                                       aCTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGAt      >  1:3362815/1‑148 (MQ=255)

                                                                                                                                       aCTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGAt      <  1:1452787/148‑1 (MQ=255)

                                                                                                                                        cTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATa     <  1:2813571/148‑1 (MQ=255)

                                                                                                                                        cTGGTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATa     <  2:2689000/148‑1 (MQ=255)

                                                                                                                                           gTCTGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATc  <  1:1158640/148‑1 (MQ=255)

CTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATC  >  CP009273/3969568‑3969854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`