Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,996,346 T→A 100% I70F (ATC→TTC)  yigG ← PRK11371 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,996,3460TA98.4% 801.4 / 1.6 247I70F (ATC→TTC) yigGPRK11371 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base A (119/124);  total (121/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 255 total aligned reads displayed.

AGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAA  >  CP009273/3996201‑3996490
                                                                                                                                                 |                                                                                                                                                
aGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATagaaga                                                                                                                                                <  1:2429507/148‑1 (MQ=255)
 gCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAgaagaa                                                                                                                                               <  2:665420/148‑1 (MQ=255)
  cATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGaaa                                                                                                                                              >  1:153056/1‑148 (MQ=255)
     tAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCa                                                                                                                                           >  2:2648745/1‑148 (MQ=255)
        cATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTa                                                                                                                                        <  1:536700/148‑1 (MQ=255)
        cATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTa                                                                                                                                        >  2:834089/1‑148 (MQ=255)
        cATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTa                                                                                                                                        >  2:1584341/1‑148 (MQ=255)
          tACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATc                                                                                                                                      >  1:1299087/1‑148 (MQ=255)
          tACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATc                                                                                                                                      >  1:1297832/1‑148 (MQ=255)
              gacaGTGTAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGTCAAAATCTTCGTCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACgg                                                                                                                                  <  2:2162559/145‑1 (MQ=255)
               aaaGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGa                                                                                                                                 <  1:668909/148‑1 (MQ=255)
                aaGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAg                                                                                                                                >  1:2467898/1‑148 (MQ=255)
                 aGTTGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGt                                                                                                                               <  2:3163913/148‑1 (MQ=255)
                 aGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGt                                                                                                                               >  2:1941788/1‑148 (MQ=255)
                 aGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGt                                                                                                                               >  1:1126951/1‑148 (MQ=255)
                 aGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGt                                                                                                                               >  1:997857/1‑148 (MQ=255)
                   tGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATAATGCTAATTTAAAAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCg                                                                                                                             >  2:2105145/1‑148 (MQ=255)
                    ggAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGc                                                                                                                            <  2:812773/148‑1 (MQ=255)
                     gAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCt                                                                                                                           >  1:1990515/1‑148 (MQ=255)
                      aaaaaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAATTCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTg                                                                                                                          <  2:2357172/148‑1 (MQ=255)
                      aaaaaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTg                                                                                                                          >  1:2146253/1‑148 (MQ=255)
                      aaaaaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTg                                                                                                                          <  1:2985602/148‑1 (MQ=255)
                       aaaaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTgg                                                                                                                         >  1:1861262/1‑148 (MQ=255)
                          aTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATAGAGAAGAAACCACTATCACGGAGTCGCTGGCaa                                                                                                                      >  1:2654191/1‑148 (MQ=255)
                           tAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAt                                                                                                                     <  1:2755701/148‑1 (MQ=255)
                            aaCAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAtt                                                                                                                    >  2:1044652/1‑148 (MQ=255)
                             aCAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTc                                                                                                                   >  2:3231938/1‑148 (MQ=255)
                             aCAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTc                                                                                                                   <  2:2484499/148‑1 (MQ=255)
                             aCAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTc                                                                                                                   <  2:2484437/148‑1 (MQ=255)
                               aaaTCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCAt                                                                                                                 <  2:3454765/148‑1 (MQ=255)
                                   cAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtg                                                                                                             <  2:2068170/148‑1 (MQ=255)
                                    aaaaaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtga                                                                                                            <  1:2614243/148‑1 (MQ=255)
                                     aaaaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATGTATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtgat                                                                                                           >  1:947247/1‑148 (MQ=255)
                                     aaaaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtgtt                                                                                                           >  1:1026538/1‑146 (MQ=255)
                                      aaaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtgatg                                                                                                          <  2:1769849/148‑1 (MQ=255)
                                       aaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtgatga                                                                                                         <  2:1858597/148‑1 (MQ=255)
                                            catcTAACAATTGCCTGAACAGGCAAAATCTTCGGCTATCATTTTGATGATATAGATTATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACgaga                                                                                                    <  2:3284584/148‑1 (MQ=255)
                                              tcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATa                                                                                                  <  1:1584341/148‑1 (MQ=255)
                                               cGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCCATTAATTTTTATGACGCGTTaa                                                                                                 >  1:1517902/1‑148 (MQ=255)
                                                 aaCCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTGATGTTGATGACGAGATAATg                                                                                               >  1:80284/1‑148 (MQ=255)
                                                  aCCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTTATGACGCGATAATgg                                                                                              >  2:1696890/1‑148 (MQ=255)
                                                    cATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAg                                                                                            <  1:300903/148‑1 (MQ=255)
                                                        gCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACg                                                                                        <  1:334301/148‑1 (MQ=255)
                                                          cTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAATCATGTTGATGACGAGATAATGGAGTACGAt                                                                                      >  1:204937/1‑148 (MQ=255)
                                                            gAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATgg                                                                                    <  2:330787/148‑1 (MQ=255)
                                                               cAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTag                                                                                 <  2:1604619/148‑1 (MQ=255)
                                                                aGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTaga                                                                                <  1:391609/148‑1 (MQ=255)
                                                                  gCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAgaga                                                                              >  2:1528864/1‑148 (MQ=255)
                                                                     aaaTCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACta                                                                           >  2:319630/1‑148 (MQ=255)
                                                                      aaTCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACtat                                                                          <  1:1229123/148‑1 (MQ=255)
                                                                         cTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAAc                                                                       >  2:27086/1‑148 (MQ=255)
                                                                         cTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAAc                                                                       <  2:2621811/148‑1 (MQ=255)
                                                                         cTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAAc                                                                       <  2:863059/148‑1 (MQ=255)
                                                                           tCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACaa                                                                     >  1:3146701/1‑148 (MQ=255)
                                                                                  tCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGcc                                                              <  2:2300813/148‑1 (MQ=255)
                                                                                  tCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAATCATGTTGTTTTCGTGATAATTGAGTACGATGGTAGAGACTATAACAACAAAGcc                                                              >  1:4298/1‑148 (MQ=255)
                                                                                    aTTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTg                                                            <  2:2146253/148‑1 (MQ=255)
                                                                                    aTTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTg                                                            <  1:286627/148‑1 (MQ=255)
                                                                                     ttGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAATTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGATTACGATGGTAGAGACTATAACAAGAAAGCCTGc                                                           <  2:2568678/148‑1 (MQ=255)
                                                                                         gatgatAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTtct                                                       >  2:2002207/1‑148 (MQ=255)
                                                                                          atgatAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTtctc                                                      >  2:2997673/1‑148 (MQ=255)
                                                                                           tgatAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTcc                                                     <  1:2687374/148‑1 (MQ=255)
                                                                                             atAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCAt                                                   >  1:169207/1‑148 (MQ=255)
                                                                                               agagATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAACCCAGCTTATCCaaag                                                 >  2:612869/1‑145 (MQ=255)
                                                                                                gagaTGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGt                                                <  1:180726/148‑1 (MQ=255)
                                                                                                 agaTGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGtt                                               >  2:3425956/1‑148 (MQ=255)
                                                                                                   atgatATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATTACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaa                                             >  1:2195315/1‑148 (MQ=255)
                                                                                                     gatATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaaa                                           <  2:3326929/148‑1 (MQ=255)
                                                                                                      atatatACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaaaa                                          <  1:1941788/148‑1 (MQ=255)
                                                                                                       tatataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAATCATGTTGATGACGAGATAATGGAGTAAGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaaaaa                                         >  2:3305895/1‑148 (MQ=255)
                                                                                                        atataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGTCGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAg                                        >  2:419794/1‑148 (MQ=255)
                                                                                                        atataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAg                                        >  2:2327978/1‑148 (MQ=255)
                                                                                                           taCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGata                                     <  1:3511027/148‑1 (MQ=255)
                                                                                                            aCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAATCCTGCGTCTCCATCGTTAAAAAAGataa                                    >  2:1966616/1‑148 (MQ=255)
                                                                                                            aCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGataa                                    <  2:96682/148‑1 (MQ=255)
                                                                                                            aCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGataa                                    <  2:947247/148‑1 (MQ=255)
                                                                                                            aCTGATAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGataa                                    <  2:257918/148‑1 (MQ=255)
                                                                                                             cTGCTAATGTACAAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGataat                                   <  1:3262874/148‑1 (MQ=255)
                                                                                                                 tAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGa                               <  1:3231938/148‑1 (MQ=255)
                                                                                                                 tAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGa                               >  1:831517/1‑148 (MQ=255)
                                                                                                                 tAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGa                               <  1:2099645/148‑1 (MQ=255)
                                                                                                                 tAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGa                               <  2:1126951/148‑1 (MQ=255)
                                                                                                                  aaTGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATTTCGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCGGCGTCACCCACGTTAAAAAAGATAATAAGaa                              >  1:1874550/1‑148 (MQ=255)
                                                                                                                  aaTGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGaa                              <  1:2274254/148‑1 (MQ=255)
                                                                                                                  aaTGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGaa                              >  2:706829/1‑148 (MQ=255)
                                                                                                                   aTGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGaaa                             >  2:2522427/1‑148 (MQ=255)
                                                                                                                          aaaaaCATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTTATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAATATAATAAGAAAGGCaaaa                      >  2:14216/1‑148 (MQ=255)
                                                                                                                          aaaaaCATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAGAGATAATAAGAAAGGCaaaa                      <  1:959532/148‑1 (MQ=255)
                                                                                                                          aaaaaCATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCaaaa                      >  2:12449/1‑148 (MQ=255)
                                                                                                                           aaaaCATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCaaaaa                     <  2:2467898/148‑1 (MQ=255)
                                                                                                                             aaCATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACTATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATg                   <  2:3175536/148‑1 (MQ=255)
                                                                                                                               cATAAGTTTTTATATATAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGaa                 <  2:3032507/148‑1 (MQ=255)
                                                                                                                                aTAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAATAATGTTGATTACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTTAAAAAGATAATAAGAAAGGCAAAAATTaaa                >  1:1065238/1‑148 (MQ=255)
                                                                                                                                    gTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATtta            >  2:3355627/1‑148 (MQ=255)
                                                                                                                                    gTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAAAAAGAAAGCCTGCTTCTCCAACGTTAAAAAATATAATAAGAAAGGCAAAAATGAAATtta            >  2:901919/1‑148 (MQ=255)
                                                                                                                                          atataGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTaaaa      <  2:1299087/148‑1 (MQ=255)
                                                                                                                                          atataGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTaaaa      <  2:1297832/148‑1 (MQ=255)
                                                                                                                                          atataGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTaaaa      <  1:2825786/148‑1 (MQ=255)
                                                                                                                                          atataGAAGAAACCACTATCACGGAGTCGCTGGCAAATCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAATATAATAAGAAAGGCAAAAATGAAAATTATTaaaa      >  1:740047/1‑148 (MQ=255)
                                                                                                                                              agaagaAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAaaataaa  >  2:2796363/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAA  >  CP009273/3996201‑3996490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑