Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,046,172 A→T 100% K256N (AAA→AAT)  hemN → coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,046,1720AT98.8% 529.6 / ‑2.2 161K256N (AAA→AAT) hemNcoproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base T (93/66);  total (93/68)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 163 total aligned reads displayed.

GACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGC  >  CP009273/4046029‑4046316
                                                                                                                                               |                                                                                                                                                
gACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAatgatg                                                                                                                                              <  2:2995638/148‑1 (MQ=255)
 aCCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGc                                                                                                                                             >  1:3376764/1‑148 (MQ=255)
  ccTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCt                                                                                                                                            >  1:2832325/1‑148 (MQ=255)
   cTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTg                                                                                                                                           <  2:2413573/148‑1 (MQ=255)
    tGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGa                                                                                                                                          <  1:1241733/148‑1 (MQ=255)
      aTTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGAcc                                                                                                                                        <  1:2105409/148‑1 (MQ=255)
       tttACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCt                                                                                                                                       >  2:1953683/1‑148 (MQ=255)
        ttACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTg                                                                                                                                      >  1:2680618/1‑148 (MQ=255)
          aCGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGGCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGcc                                                                                                                                    >  2:553480/1‑148 (MQ=255)
               cTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTc                                                                                                                               <  2:829388/148‑1 (MQ=255)
                  ccGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCgc                                                                                                                            <  2:1883967/148‑1 (MQ=255)
                        cAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCaaa                                                                                                                      >  1:3101009/1‑148 (MQ=255)
                          gACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCaaaaa                                                                                                                    <  1:68658/148‑1 (MQ=255)
                           aCGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAAc                                                                                                                   >  2:3400130/1‑148 (MQ=255)
                           aCGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAAc                                                                                                                   >  2:2671285/1‑148 (MQ=255)
                            cGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACt                                                                                                                  <  2:3024903/148‑1 (MQ=255)
                              ccGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCg                                                                                                                <  1:2682085/148‑1 (MQ=255)
                              ccGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCg                                                                                                                <  2:763194/148‑1 (MQ=255)
                              ccGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCg                                                                                                                >  1:853541/1‑148 (MQ=255)
                               cGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGa                                                                                                               <  1:2513517/148‑1 (MQ=255)
                                 gagaGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGata                                                                                                             >  1:2430818/1‑148 (MQ=255)
                                  agagTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGatat                                                                                                            <  1:2574554/148‑1 (MQ=255)
                                  agagTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGatat                                                                                                            >  2:1145032/1‑148 (MQ=255)
                                             tttACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGaaa                                                                                                 >  1:2717709/1‑148 (MQ=255)
                                             tttACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGaaa                                                                                                 <  1:1548963/148‑1 (MQ=255)
                                             tttACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGaaa                                                                                                 <  1:1549593/148‑1 (MQ=255)
                                             tttACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATAAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGaaa                                                                                                 >  1:2717684/1‑148 (MQ=255)
                                               tACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAAcc                                                                                               <  2:2680618/148‑1 (MQ=255)
                                                aCCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCa                                                                                              <  2:221563/148‑1 (MQ=255)
                                                aCCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCa                                                                                              <  2:3201306/148‑1 (MQ=255)
                                                   cTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCg                                                                                           >  2:744204/1‑148 (MQ=255)
                                                       aaCGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCCGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCtt                                                                                       >  2:1417181/1‑148 (MQ=255)
                                                       aaCGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCtt                                                                                       >  2:1417148/1‑148 (MQ=255)
                                                        aCGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTc                                                                                      <  1:3232046/148‑1 (MQ=255)
                                                        aCGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTc                                                                                      <  2:2829648/148‑1 (MQ=255)
                                                         cGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTcc                                                                                     >  2:3500596/1‑148 (MQ=255)
                                                           tgtgGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTg                                                                                   <  1:2671285/148‑1 (MQ=255)
                                                                cGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCa                                                                              >  1:542356/1‑148 (MQ=255)
                                                                  gAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAAt                                                                            <  2:834426/148‑1 (MQ=255)
                                                                  gAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAAt                                                                            <  2:739233/148‑1 (MQ=255)
                                                                      tGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCggg                                                                        <  1:3236056/148‑1 (MQ=255)
                                                                          ccccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTAt                                                                    <  2:3421305/148‑1 (MQ=255)
                                                                          ccccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTAt                                                                    <  2:2577017/148‑1 (MQ=255)
                                                                          ccccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTAt                                                                    <  1:198955/148‑1 (MQ=255)
                                                                              gATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGt                                                                >  1:2048389/1‑148 (MQ=255)
                                                                               aTCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGtt                                                               <  1:3200697/148‑1 (MQ=255)
                                                                               aTCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGtt                                                               >  2:1878646/1‑148 (MQ=255)
                                                                                tCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGttt                                                              <  1:892461/148‑1 (MQ=255)
                                                                                 cGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTa                                                             >  2:1562654/1‑148 (MQ=255)
                                                                                   tCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATc                                                           >  2:342738/1‑148 (MQ=255)
                                                                                       aGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTa                                                       <  1:691614/148‑1 (MQ=255)
                                                                                       aGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTa                                                       <  1:2910011/148‑1 (MQ=255)
                                                                                       aGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCACAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCATCAGGAAACCAACGCCATCCTGACGCAATCGGGCTATCAGTTTATCGGTa                                                       >  2:138718/1‑148 (MQ=255)
                                                                                         tgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATg                                                     >  1:3495583/1‑148 (MQ=255)
                                                                                         tgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATg                                                     >  1:3494871/1‑148 (MQ=255)
                                                                                         tgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATg                                                     >  1:3052754/1‑148 (MQ=255)
                                                                                         tgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATg                                                     >  1:1757225/1‑148 (MQ=255)
                                                                                            cTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGAt                                                  >  1:2225276/1‑148 (MQ=255)
                                                                                            cTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGAt                                                  >  1:2534641/1‑148 (MQ=255)
                                                                                             tttAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCGGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATc                                                 <  2:2442420/148‑1 (MQ=255)
                                                                                             tttAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATc                                                 <  2:1476098/148‑1 (MQ=255)
                                                                                             tttAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATc                                                 >  2:2112082/1‑148 (MQ=255)
                                                                                              ttAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCa                                                <  2:1137109/148‑1 (MQ=255)
                                                                                              ttAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCGGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCa                                                <  2:854413/148‑1 (MQ=255)
                                                                                                 aCTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACtt                                             >  1:3073369/1‑148 (MQ=255)
                                                                                                  cTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACttt                                            >  1:2866339/1‑148 (MQ=255)
                                                                                                  cTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACttt                                            >  2:3322810/1‑148 (MQ=255)
                                                                                                  cTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACttt                                            >  2:239428/1‑148 (MQ=255)
                                                                                                   tACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTg                                           <  2:2854150/148‑1 (MQ=255)
                                                                                                       cgcATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCg                                       >  2:3297151/1‑148 (MQ=255)
                                                                                                           tCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCg                                   >  1:333262/1‑148 (MQ=255)
                                                                                                           tCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCg                                   >  1:570067/1‑148 (MQ=255)
                                                                                                           tCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCg                                   >  1:976267/1‑148 (MQ=255)
                                                                                                               ccGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATg                               >  1:860199/1‑148 (MQ=255)
                                                                                                                  aCCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACg                            <  2:315512/148‑1 (MQ=255)
                                                                                                                   ccATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGa                           >  2:176808/1‑148 (MQ=255)
                                                                                                                   ccATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGa                           >  2:177273/1‑148 (MQ=255)
                                                                                                                    cATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAg                          <  1:478955/148‑1 (MQ=255)
                                                                                                                     aTTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGc                         <  2:1270780/148‑1 (MQ=255)
                                                                                                                      tttttGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCt                        >  1:1516017/1‑148 (MQ=255)
                                                                                                                      tttttGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCt                        >  2:2592972/1‑148 (MQ=255)
                                                                                                                      tttttGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCt                        >  1:3259225/1‑148 (MQ=255)
                                                                                                                       ttttGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTg                       >  1:999477/1‑148 (MQ=255)
                                                                                                                        tttGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTgg                      <  1:430494/148‑1 (MQ=255)
                                                                                                                          tgctgcTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCg                    >  2:2198585/1‑148 (MQ=255)
                                                                                                                          tgctgcTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCg                    >  2:2199083/1‑148 (MQ=255)
                                                                                                                             tgctCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTg                 <  1:1435654/148‑1 (MQ=255)
                                                                                                                                 cAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGccc             <  1:2972064/148‑1 (MQ=255)
                                                                                                                                 cAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGccc             >  1:1215732/1‑148 (MQ=255)
                                                                                                                                   gcgcAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAg           <  1:2954022/148‑1 (MQ=255)
                                                                                                                                    cgcAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGc          >  1:1396917/1‑148 (MQ=255)
                                                                                                                                     gcAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCg         <  1:2562725/148‑1 (MQ=255)
                                                                                                                                      cAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGt        <  2:3228629/148‑1 (MQ=255)
                                                                                                                                      cAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGt        >  2:374536/1‑148 (MQ=255)
                                                                                                                                         aaTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGaa     >  1:1902084/1‑148 (MQ=255)
                                                                                                                                          aTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGCAg    >  2:2163103/1‑148 (MQ=255)
                                                                                                                                          aTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAg    >  2:457220/1‑148 (MQ=255)
                                                                                                                                           tCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAgg   <  2:3514734/148‑1 (MQ=255)
                                                                                                                                           tCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAgg   >  2:1756462/1‑148 (MQ=255)
                                                                                                                                            cAATGATGCAGACCTGCCGACACAGCAGCAAAAACTCGATATCCAGCAGGAAACAATAGCATTCCTGACGCAATCGGGATATCAGTTTATAGGTATGGATCACTTTGCCCGACAGGATGACTAGCTGGAGCTGGCCCAGCGTGacgtc  >  2:2100421/1‑144 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGC  >  CP009273/4046029‑4046316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑