Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,059,655 T→A 5.2% S300S (TCA→TCT)  yihP ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,059,6550TA 5.2% 658.9 / 19.1 210S300S (TCA→TCT) yihPputative transporter
Reads supporting (aligned to +/- strand):  ref base T (95/104);  new base A (5/6);  total (100/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 223 total aligned reads displayed.

GTTAACAAACGCTGAGCCAAAGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGACGTTAAACGCCCCTAAAGTGCAGAG  >  CP009273/4059508‑4059801
                                                                                                                                                   |                                                                                                                                                  
gTTAACAAACGCTGAGCCAAAGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTctgact                                                                                                                                                    >  1:3267106/1‑148 (MQ=255)
gTTAACAAACGCTGAGCCAAAGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTctgact                                                                                                                                                    >  2:224185/1‑148 (MQ=255)
 ttAACAAACGCTGAGCCAAAGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTctgactg                                                                                                                                                   >  2:3513652/1‑148 (MQ=255)
  tAACAAACGCTGAGCCAAAGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTctgactga                                                                                                                                                  >  2:2426198/1‑148 (MQ=255)
       aaCGCTGAGCCAAAGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGaggcag                                                                                                                                             >  1:1567359/1‑148 (MQ=255)
        ccGCTGAGCCAAAGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACAGaggcagg                                                                                                                                            <  2:1500688/147‑1 (MQ=255)
         cGCTGAGCCAAAGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGaggcaggc                                                                                                                                           <  2:2869577/148‑1 (MQ=255)
            tGAGCCAAAGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATc                                                                                                                                        <  2:1897835/148‑1 (MQ=255)
            tGAGCCAAAGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATc                                                                                                                                        <  2:2603826/148‑1 (MQ=255)
             gAGCCACAGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCa                                                                                                                                       <  2:1663167/148‑1 (MQ=255)
             gAGCCAAAGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCa                                                                                                                                       >  1:1295873/1‑148 (MQ=255)
               gCCAAAGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAgg                                                                                                                                     >  1:2331641/1‑148 (MQ=255)
               gCCAAAGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAgg                                                                                                                                     <  2:1567838/148‑1 (MQ=255)
                ccAAAGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGa                                                                                                                                    >  1:51740/1‑148 (MQ=255)
                  aaaGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAAt                                                                                                                                  <  1:2044186/148‑1 (MQ=255)
                  aaaGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAAt                                                                                                                                  >  1:690076/1‑148 (MQ=255)
                   aaGAACGACAGGCATGAGAACGCCACGAATCTGACCGAACCGAAGAAGAAGAAATAGTTTAGCATATCGCACAGAACCCAAATCAGCAGTCAGCCGATATAAAATTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGTAATa                                                                                                                                 <  1:3198171/148‑1 (MQ=255)
                    aGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATAc                                                                                                                                >  2:1793331/1‑148 (MQ=255)
                          ccAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGAt                                                                                                                          <  2:410949/148‑1 (MQ=255)
                            aggcaggAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGa                                                                                                                        >  1:322107/1‑148 (MQ=255)
                             ggcaggAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGaa                                                                                                                       >  1:2638784/1‑148 (MQ=255)
                              gcaggAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGaaa                                                                                                                      <  1:1033878/148‑1 (MQ=255)
                              gcaggAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGaaa                                                                                                                      <  1:404275/148‑1 (MQ=255)
                               caggAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACAGAGGCAGGCATCAGGAATACGCCGATGaaaa                                                                                                                     <  1:1015914/148‑1 (MQ=255)
                                  gagaACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATAc                                                                                                                  <  1:210897/148‑1 (MQ=255)
                                  gagaACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATAc                                                                                                                  <  1:1480045/148‑1 (MQ=255)
                                    gaACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAg                                                                                                                >  2:526352/1‑148 (MQ=255)
                                    gaACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAg                                                                                                                <  2:2641159/148‑1 (MQ=255)
                                     aaCGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGc                                                                                                               >  1:279771/1‑148 (MQ=255)
                                      aCGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGcc                                                                                                              <  1:78796/148‑1 (MQ=255)
                                      aCGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGcc                                                                                                              <  1:660042/148‑1 (MQ=255)
                                       cGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGccc                                                                                                             >  1:3137467/1‑148 (MQ=255)
                                       cGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGccc                                                                                                             <  2:266115/148‑1 (MQ=255)
                                           aCGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGc                                                                                                         >  1:1543782/1‑148 (MQ=255)
                                              aaGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGAGGAAAATACAGCCCAtgatga                                                                                                      >  1:1089557/1‑148 (MQ=255)
                                               aGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGaa                                                                                                     >  2:1324007/1‑148 (MQ=255)
                                               aGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGaa                                                                                                     <  1:224185/148‑1 (MQ=255)
                                                   gACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGACAATACAGCCCATGCTGAAAAAt                                                                                                 >  2:3443843/1‑148 (MQ=255)
                                                    aCCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATc                                                                                                <  2:723329/148‑1 (MQ=255)
                                                        aaCCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCat                                                                                            >  2:2220099/1‑148 (MQ=255)
                                                        aaCCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACAGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCat                                                                                            >  2:2604793/1‑148 (MQ=255)
                                                         aCCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCata                                                                                           <  2:57653/148‑1 (MQ=255)
                                                         aCCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTAGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCata                                                                                           >  2:291520/1‑148 (MQ=255)
                                                          ccgccgccgAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCatat                                                                                          <  2:1909279/148‑1 (MQ=255)
                                                              cgccgAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGa                                                                                      <  2:1464811/148‑1 (MQ=255)
                                                                ccgAATAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCAGATGAAAATACAGCCCATGCTTAAAAATCCCATATACGaca                                                                                    <  2:2780630/148‑1 (MQ=255)
                                                                 cgAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGacaa                                                                                   <  2:2810696/148‑1 (MQ=255)
                                                                     gaaATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAgg                                                                               >  2:1574265/1‑148 (MQ=255)
                                                                      aaaTAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGa                                                                              >  2:902460/1‑148 (MQ=255)
                                                                      aaaTAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGa                                                                              >  1:1366505/1‑148 (MQ=255)
                                                                       aaTAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGAt                                                                             >  1:220653/1‑148 (MQ=255)
                                                                       aaTAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGAt                                                                             >  1:961795/1‑148 (MQ=255)
                                                                        aTAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATg                                                                            <  1:981912/148‑1 (MQ=255)
                                                                        aTAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATg                                                                            <  2:2046171/148‑1 (MQ=255)
                                                                         tAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATgg                                                                           >  2:534659/1‑148 (MQ=255)
                                                                         tAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATgg                                                                           >  2:534159/1‑148 (MQ=255)
                                                                           gTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGa                                                                         <  1:2780607/148‑1 (MQ=255)
                                                                           gTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGa                                                                         <  2:3366610/148‑1 (MQ=255)
                                                                           gTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGa                                                                         <  2:3430486/148‑1 (MQ=255)
                                                                           gTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGa                                                                         <  1:2453343/148‑1 (MQ=255)
                                                                           gTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACAGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGa                                                                         <  1:2913793/148‑1 (MQ=255)
                                                                                    aTCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCGGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGc                                                                >  1:2561085/1‑148 (MQ=255)
                                                                                       gCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACg                                                             >  2:2905816/1‑148 (MQ=255)
                                                                                        cccAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGt                                                            >  2:1144484/1‑148 (MQ=255)
                                                                                        cccAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGt                                                            >  2:1029553/1‑148 (MQ=255)
                                                                                         ccAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTa                                                           <  1:1240884/148‑1 (MQ=255)
                                                                                            gCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTg                                                        <  1:2922988/148‑1 (MQ=255)
                                                                                            gCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTg                                                        >  2:3097371/1‑148 (MQ=255)
                                                                                                 cAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGgtgt                                                   >  1:3243330/1‑148 (MQ=255)
                                                                                                  aaaTCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTa                                                  <  2:19894/148‑1 (MQ=255)
                                                                                                  aaaTCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTa                                                  >  1:305457/1‑148 (MQ=255)
                                                                                                   aaTCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTaa                                                 <  1:3513652/148‑1 (MQ=255)
                                                                                                      cagcagGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAg                                              >  1:1771357/1‑148 (MQ=255)
                                                                                                        gcagGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGAc                                            >  2:405479/1‑148 (MQ=255)
                                                                                                        gcagGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGAc                                            <  2:3373540/148‑1 (MQ=255)
                                                                                                         cagGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGAcc                                           >  2:1184902/1‑148 (MQ=255)
                                                                                                         cagGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACAGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGAcc                                           >  2:3332122/1‑148 (MQ=255)
                                                                                                            gccgccGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTgg                                        >  2:498074/1‑148 (MQ=255)
                                                                                                                ccgATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATgg                                    <  2:1621213/148‑1 (MQ=255)
                                                                                                                 cgATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGc                                   <  1:1984717/148‑1 (MQ=255)
                                                                                                                  gATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCg                                  >  2:869251/1‑148 (MQ=255)
                                                                                                                   atatAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGa                                 >  1:1174763/1‑148 (MQ=255)
                                                                                                                    tataAACTTTCTTCTTGCCAAAACGTCTGACAGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAg                                >  1:3484506/1‑148 (MQ=255)
                                                                                                                     ataAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGc                               >  2:3379958/1‑148 (MQ=255)
                                                                                                                     ataAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGCTGTAATAGACCTGGATGGCGAGc                               <  2:3285632/148‑1 (MQ=255)
                                                                                                                       aaaCTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCtt                             <  2:2262292/148‑1 (MQ=255)
                                                                                                                        aaCTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTg                            >  2:3266509/1‑148 (MQ=255)
                                                                                                                         aCTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGa                           <  1:3393074/148‑1 (MQ=255)
                                                                                                                         aCTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGa                           <  1:241443/148‑1 (MQ=255)
                                                                                                                         aCTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGa                           >  1:1683947/1‑148 (MQ=255)
                                                                                                                         aCTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCCTTAAGCACGTACTGGGTGTAATAGAGCTGGATGGCGAGCTTGa                           >  2:797047/1‑148 (MQ=255)
                                                                                                                            ttcttcTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGACGt                        <  1:139020/148‑1 (MQ=255)
                                                                                                                            ttcttcGTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGACGt                        <  2:2578707/148‑1 (MQ=255)
                                                                                                                              cttcttGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGACGTTa                      >  1:603785/1‑148 (MQ=255)
                                                                                                                               ttcttGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGACGTTaa                     <  2:2752699/148‑1 (MQ=255)
                                                                                                                                      cAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGACGTTAAACGcccc              >  1:165265/1‑148 (MQ=255)
                                                                                                                                              ctgactgaGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGACGTTAAACGCCCCTAAAGTGc      >  1:2360485/1‑148 (MQ=255)
                                                                                                                                              ctgactgaGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGAAAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGACGTTAAACGCCCCTAAAGTGc      <  2:3267106/148‑1 (MQ=255)
                                                                                                                                                 actgaGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGACGTTAAACGCCCCTAAAGTGCaga   >  2:1700142/1‑148 (MQ=255)
                                                                                                                                                  ctgaGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGACGTTAAACGCCCCTAAAGTGCagag  >  1:1962876/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTAACAAACGCTGAGCCAAAGAACGCCAGGCAGGAGAACGCCACGAAGCTGACCGAACCGCCGCCGAAGAAATAGTTGAGCAGATCGCCCAGCACCCAAATCAGCAGGCCGCCGATATAAACTTTCTTCTTGCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGACGTTAAACGCCCCTAAAGTGCAGAG  >  CP009273/4059508‑4059801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑