Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,074,579 G→T 100% R4S (CGC→AGC)  fdhE ← formate dehydrogenase formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,074,5790GT99.0% 688.6 / ‑1.9 212R4S (CGC→AGC) fdhEformate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (88/121);  total (90/122)
Fisher's exact test for biased strand distribution p-value = 5.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 212 total aligned reads displayed.

TCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGC  >  CP009273/4074434‑4074723
                                                                                                                                                 |                                                                                                                                                
tCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTaa                                                                                                                                                >  1:3300272/1‑148 (MQ=255)
  aCCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATa                                                                                                                                              <  2:2800932/148‑1 (MQ=255)
  aCCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATa                                                                                                                                              <  2:3342109/148‑1 (MQ=255)
  aCCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATa                                                                                                                                              <  2:2801325/148‑1 (MQ=255)
  aCCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATa                                                                                                                                              <  2:3342120/148‑1 (MQ=255)
   cccAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATAc                                                                                                                                             >  2:2849568/1‑148 (MQ=255)
     cAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTc                                                                                                                                           <  2:1040851/148‑1 (MQ=255)
     cAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTc                                                                                                                                           <  2:1899582/148‑1 (MQ=255)
       gagGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCAt                                                                                                                                         <  1:232334/148‑1 (MQ=255)
        agGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCAtt                                                                                                                                        >  1:1675987/1‑148 (MQ=255)
         ggATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTc                                                                                                                                       <  2:561491/148‑1 (MQ=255)
          gATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCa                                                                                                                                      >  1:1291976/1‑148 (MQ=255)
           attattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAg                                                                                                                                     <  1:1881035/148‑1 (MQ=255)
            ttattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTTTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGc                                                                                                                                    <  1:1345338/148‑1 (MQ=255)
            ttattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGc                                                                                                                                    <  1:1571633/148‑1 (MQ=255)
            ttattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGc                                                                                                                                    >  1:1835889/1‑148 (MQ=255)
             tattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGct                                                                                                                                   >  1:1134349/1‑148 (MQ=255)
             tattTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGct                                                                                                                                   <  1:2422805/148‑1 (MQ=255)
                tttCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttc                                                                                                                                <  1:400978/148‑1 (MQ=255)
                tttCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttc                                                                                                                                <  1:2533876/148‑1 (MQ=255)
                 ttCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttct                                                                                                                               <  2:276636/148‑1 (MQ=255)
                 ttCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttct                                                                                                                               <  1:2418410/148‑1 (MQ=255)
                  tCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttctt                                                                                                                              <  2:3347330/148‑1 (MQ=255)
                  tCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGctttctt                                                                                                                              <  2:3485710/148‑1 (MQ=255)
                        aGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGt                                                                                                                        <  2:59058/148‑1 (MQ=255)
                         gCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGACTCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGtt                                                                                                                       >  1:878428/1‑148 (MQ=255)
                          cTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTg                                                                                                                      >  2:178537/1‑148 (MQ=255)
                           tCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGt                                                                                                                     <  1:1423425/148‑1 (MQ=255)
                           tCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTACGTTGt                                                                                                                     >  2:1805024/1‑148 (MQ=255)
                            cgcgCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTc                                                                                                                    <  1:826028/148‑1 (MQ=255)
                             gcgcAGACGCTCGTCGCGGCGGTTGTATATATTCTTGAGCCGAGGGTACAATTACTGCGGAATCATATCCGCCGTACGTTTGTCGCTTGCACCCATCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCt                                                                                                                   <  2:891352/148‑1 (MQ=255)
                              cgcAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCtt                                                                                                                  >  1:1183622/1‑148 (MQ=255)
                               gcAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTg                                                                                                                 <  2:1675987/148‑1 (MQ=255)
                                   aCGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGa                                                                                                             >  1:3344821/1‑148 (MQ=255)
                                     gCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGTAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGAcc                                                                                                           <  1:1247978/148‑1 (MQ=255)
                                       tCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTc                                                                                                         <  2:3300272/148‑1 (MQ=255)
                                        cggcgcggcgGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCa                                                                                                        >  2:2964110/1‑148 (MQ=255)
                                           cgcggcgGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACgg                                                                                                     <  1:3371007/148‑1 (MQ=255)
                                            gcggcgGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGt                                                                                                    <  1:283538/148‑1 (MQ=255)
                                              ggcggTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTAc                                                                                                  <  1:2792748/148‑1 (MQ=255)
                                               gcggTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTc                                                                                                         >  2:2518200/1‑140 (MQ=255)
                                               gcggTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTAcc                                                                                                 <  2:512062/148‑1 (MQ=255)
                                                 ggTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAg                                                                                               <  1:1697690/148‑1 (MQ=255)
                                                        taAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGt                                                                                        <  1:428399/148‑1 (MQ=255)
                                                         aaaTTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTg                                                                                       <  2:2135964/148‑1 (MQ=255)
                                                         aaaTTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTg                                                                                       <  2:2135235/148‑1 (MQ=255)
                                                           aTTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGAt                                                                                     <  2:2783419/148‑1 (MQ=255)
                                                            ttCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATg                                                                                    <  2:1145948/148‑1 (MQ=255)
                                                             tCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGt                                                                                   <  1:2150985/148‑1 (MQ=255)
                                                             tCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGt                                                                                   >  1:2829758/1‑148 (MQ=255)
                                                                 gAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTttct                                                                               <  2:260255/148‑1 (MQ=255)
                                                                  aGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTttctt                                                                              <  1:2025731/148‑1 (MQ=255)
                                                                  aGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTttctt                                                                              <  1:2026719/148‑1 (MQ=255)
                                                                      gAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGcc                                                                          <  1:816305/148‑1 (MQ=255)
                                                                      gAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGcc                                                                          <  1:2876240/148‑1 (MQ=255)
                                                                       aGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGccc                                                                         >  1:714699/1‑148 (MQ=255)
                                                                       aGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGccc                                                                         >  2:2369024/1‑148 (MQ=255)
                                                                       aGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGccc                                                                         <  1:97019/148‑1 (MQ=255)
                                                                             cAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGgcgc                                                                   <  2:424012/148‑1 (MQ=255)
                                                                             cAAGAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGgcgc                                                                   <  2:2697377/148‑1 (MQ=255)
                                                                                tAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTgg                                                                >  1:3009633/1‑148 (MQ=255)
                                                                                tAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTgg                                                                >  2:668713/1‑148 (MQ=255)
                                                                                tAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTgg                                                                >  2:2371842/1‑148 (MQ=255)
                                                                                tAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTgg                                                                >  2:391340/1‑148 (MQ=255)
                                                                                tAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTgg                                                                >  1:1261218/1‑148 (MQ=255)
                                                                                   cggcggAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTaa                                                             >  2:2892318/1‑148 (MQ=255)
                                                                                        gAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCAt                                                        >  2:1837682/1‑148 (MQ=255)
                                                                                        gAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCAt                                                        >  2:2021225/1‑148 (MQ=255)
                                                                                         aaTCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATc                                                       <  1:432549/148‑1 (MQ=255)
                                                                                          aTCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATcc                                                      >  2:3077862/1‑148 (MQ=255)
                                                                                            cATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCtt                                                    >  2:271409/1‑148 (MQ=255)
                                                                                              tatCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTcc                                                  <  2:3420403/148‑1 (MQ=255)
                                                                                                tCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTAccac                                                >  1:3170458/1‑148 (MQ=255)
                                                                                                   gccgTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCAt                                             >  2:2832651/1‑148 (MQ=255)
                                                                                                       tACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCg                                         >  1:2458659/1‑148 (MQ=255)
                                                                                                            ttCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAAt                                    >  1:1101603/1‑148 (MQ=255)
                                                                                                            ttCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAAt                                    >  2:3176663/1‑148 (MQ=255)
                                                                                                              ctcGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCg                                  >  2:1490098/1‑148 (MQ=255)
                                                                                                              ctcGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCg                                  <  1:3030263/148‑1 (MQ=255)
                                                                                                                cGCTTGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTg                                >  2:9583/1‑148 (MQ=255)
                                                                                                                   ttGAACCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCt                             <  2:2434995/148‑1 (MQ=255)
                                                                                                                     gAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCttt                           >  2:1247962/1‑148 (MQ=255)
                                                                                                                       aCCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCa                         >  1:1019350/1‑148 (MQ=255)
                                                                                                                       aCCCAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCa                         >  1:1531291/1‑148 (MQ=255)
                                                                                                                         ccAGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCAcc                       <  1:1652543/148‑1 (MQ=255)
                                                                                                                           aGCTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCa                     >  1:370702/1‑148 (MQ=255)
                                                                                                                             cTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCaaa                   >  2:1004050/1‑148 (MQ=255)
                                                                                                                             cTCATCTTGCGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCaaa                   <  1:2731555/148‑1 (MQ=255)
                                                                                                                                      cGGGATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGt          <  1:763745/148‑1 (MQ=255)
                                                                                                                                        ggATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAg        >  2:1586736/1‑148 (MQ=255)
                                                                                                                                        ggATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAg        <  2:1134349/148‑1 (MQ=255)
                                                                                                                                        ggATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAg        <  1:1797569/148‑1 (MQ=255)
                                                                                                                                         gATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGa       >  2:552861/1‑148 (MQ=255)
                                                                                                                                         gATTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGa       <  2:3083637/148‑1 (MQ=255)
                                                                                                                                          aTTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGat      >  1:2697997/1‑148 (MQ=255)
                                                                                                                                          aTTATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGat      >  2:3194035/1‑148 (MQ=255)
                                                                                                                                            tATGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGatat    >  2:2259297/1‑148 (MQ=255)
                                                                                                                                             aTGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATg   >  2:2889577/1‑148 (MQ=255)
                                                                                                                                             aTGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATg   >  2:839567/1‑148 (MQ=255)
                                                                                                                                              tGCTAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTGGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGc  <  1:1405106/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCCGCCGTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGC  >  CP009273/4074434‑4074723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑