BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,097,822	G→T	100%	E115* (GAA→TAA)	pfkA →	6‑phosphofructokinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,097,822	0	G	T	100.0%	646.5 / NA	195	E115* (GAA→TAA)	pfkA	6‑phosphofructokinase I
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (100/95); total (100/95)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 195 total aligned reads displayed.

TACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGC  >  CP009273/4097677‑4097966

tACGGTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTaa                                                                                                                                                >  2:242123/1‑148 (MQ=255)

  cGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAAt                                                                                                                                              <  1:2574854/148‑1 (MQ=255)

     tCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATggg                                                                                                                                           >  2:1966758/1‑148 (MQ=255)

     tCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATggg                                                                                                                                           >  2:193009/1‑148 (MQ=255)

     tCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATggg                                                                                                                                           >  1:1705712/1‑148 (MQ=255)

      ccTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGc                                                                                                                                          >  1:2097827/1‑148 (MQ=255)

       cTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCt                                                                                                                                         >  1:456907/1‑148 (MQ=255)

        tCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCtt                                                                                                                                        <  2:672486/148‑1 (MQ=255)

                gcgcGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCa                                                                                                                                >  1:974953/1‑148 (MQ=255)

                gcgcGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCa                                                                                                                                >  1:1378827/1‑148 (MQ=255)

                     tttCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGt                                                                                                                           <  2:456907/148‑1 (MQ=255)

                     tttCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGt                                                                                                                           >  2:720316/1‑148 (MQ=255)

                     tttCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGt                                                                                                                           >  2:1178245/1‑148 (MQ=255)

                        cccGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTg                                                                                                                        >  1:1796475/1‑148 (MQ=255)

                                tCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCAc                                                                                                                >  1:1403719/1‑148 (MQ=255)

                                tCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCAc                                                                                                                >  2:2646579/1‑148 (MQ=255)

                                 ccGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACt                                                                                                               <  2:2722184/148‑1 (MQ=255)

                                  cgcgACGAGAACATCCGCGCCGTGGCTATCGAACACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTa                                                                                                              <  2:2201029/148‑1 (MQ=255)

                                   gcgACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTAt                                                                                                             >  2:2603139/1‑148 (MQ=255)

                                   gcgACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTAt                                                                                                             <  1:493410/148‑1 (MQ=255)

                                    cgacgaGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATc                                                                                                            >  1:3221328/1‑148 (MQ=255)

                                     gacgaGAACATCCGCGCCGTGGCTATCGAAAACCTGACAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCg                                                                                                           <  1:1498904/148‑1 (MQ=255)

                                     gacgaGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCg                                                                                                           <  1:1499072/148‑1 (MQ=255)

                                      acgaGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGTCCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGa                                                                                                          >  1:609122/1‑148 (MQ=255)

                                      acgaGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGa                                                                                                          >  2:779828/1‑148 (MQ=255)

                                        gagaACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACa                                                                                                        <  1:168362/148‑1 (MQ=255)

                                        gagaACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACa                                                                                                        <  2:2559612/148‑1 (MQ=255)

                                        gagaACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACa                                                                                                        <  2:2972818/148‑1 (MQ=255)

                                        gagaACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACa                                                                                                        <  1:1172582/148‑1 (MQ=255)

                                        gagaACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACa                                                                                                        <  2:1222026/148‑1 (MQ=255)

                                          gaACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAAc                                                                                                      >  1:1045918/1‑148 (MQ=255)

                                            aCATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGa                                                                                                    >  1:2473620/1‑148 (MQ=255)

                                             cATCCTCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGAc                                                                                                   <  2:173576/148‑1 (MQ=255)

                                             cATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGAc                                                                                                   >  1:1759912/1‑148 (MQ=255)

                                               tCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACAt                                                                                                 >  2:2840141/1‑148 (MQ=255)

                                               tCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACAt                                                                                                 >  2:815391/1‑148 (MQ=255)

                                                ccGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATc                                                                                                >  2:556787/1‑148 (MQ=255)

                                                  gcgcCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCaa                                                                                              <  1:55836/148‑1 (MQ=255)

                                                   cgcCGTGGCTATCGAAAACCTGAGAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCaaa                                                                                             >  1:2875114/1‑148 (MQ=255)

                                                   cgcCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCaaa                                                                                             <  2:1921385/148‑1 (MQ=255)

                                                   cgcCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCaaa                                                                                             >  2:854287/1‑148 (MQ=255)

                                                       gTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCa                                                                                         <  2:1476603/148‑1 (MQ=255)

                                                        tGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCAc                                                                                        <  1:3189322/148‑1 (MQ=255)

                                                         ggCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACt                                                                                       <  2:1844226/148‑1 (MQ=255)

                                                         ggCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACt                                                                                       >  1:881878/1‑148 (MQ=255)

                                                           cTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGa                                                                                     >  2:3346066/1‑148 (MQ=255)

                                                             aTCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACt                                                                                   <  1:3268493/148‑1 (MQ=255)

                                                              tCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTa                                                                                  <  1:582833/148‑1 (MQ=255)

                                                              tCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTa                                                                                  <  2:3219156/148‑1 (MQ=255)

                                                                   aaCCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTa                                                                             <  2:3388265/148‑1 (MQ=255)

                                                                   aaCCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTa                                                                             >  1:352978/1‑148 (MQ=255)

                                                                   aaCCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTa                                                                             >  1:1563410/1‑148 (MQ=255)

                                                                   aaCCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCGGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTa                                                                             >  1:2069616/1‑148 (MQ=255)

                                                                      cTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCg                                                                          >  1:855357/1‑148 (MQ=255)

                                                                       tGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCgg                                                                         >  2:919246/1‑148 (MQ=255)

                                                                       tGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCgg                                                                         <  1:797770/148‑1 (MQ=255)

                                                                       tGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCgg                                                                         <  2:2043159/148‑1 (MQ=255)

                                                                        gAAAACACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGt                                                                        <  2:2012815/148‑1 (MQ=255)

                                                                           aaaaCGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTttc                                                                     >  2:2755834/1‑148 (MQ=255)

                                                                            aaaCGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGCCGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTttct                                                                    <  1:2183027/148‑1 (MQ=255)

                                                                            aaaCGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTttct                                                                    >  1:3464754/1‑148 (MQ=255)

                                                                             taCGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTttctt                                                                   <  2:1018867/147‑1 (MQ=255)

                                                                              aCGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTttcttc                                                                  <  2:2487494/148‑1 (MQ=255)

                                                                               cGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCa                                                                 <  1:2840141/148‑1 (MQ=255)

                                                                               cGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCa                                                                 <  1:1371969/148‑1 (MQ=255)

                                                                               cGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCa                                                                 >  1:2155493/1‑148 (MQ=255)

                                                                               cGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCa                                                                 <  2:3418099/148‑1 (MQ=255)

                                                                                     aTCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTgcgc                                                           >  2:1321324/1‑148 (MQ=255)

                                                                                      tCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCt                                                          >  1:1642529/1‑148 (MQ=255)

                                                                                       cGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTg                                                         <  1:2940196/148‑1 (MQ=255)

                                                                                       cGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTg                                                         >  1:1899001/1‑148 (MQ=255)

                                                                                          cgcgCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGc                                                      <  1:62243/148‑1 (MQ=255)

                                                                                                 gtggtTATCGGCGGTGACGGTTCCTACATGGGTGCAATGTGTCTGACCTAAATGGGCGTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTg                                               <  1:3306529/148‑1 (MQ=255)

                                                                                                 gtggtTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTg                                               >  1:325946/1‑148 (MQ=255)

                                                                                                 gtggtTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTg                                               >  2:2646101/1‑148 (MQ=255)

                                                                                                         cggcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCg                                       >  1:2635583/1‑148 (MQ=255)

                                                                                                          ggcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGa                                      <  2:2941404/148‑1 (MQ=255)

                                                                                                          ggcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGa                                      <  2:881878/148‑1 (MQ=255)

                                                                                                              gTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGa                                  >  1:654696/1‑148 (MQ=255)

                                                                                                               tGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGAc                                 <  2:1705712/148‑1 (MQ=255)

                                                                                                                 aCGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCg                               >  2:2226986/1‑148 (MQ=255)

                                                                                                                  cGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGt                              >  2:1552573/1‑148 (MQ=255)

                                                                                                                    gTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCt                            >  1:682823/1‑148 (MQ=255)

                                                                                                                    gTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCt                            >  1:2258377/1‑148 (MQ=255)

                                                                                                                     ttCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTg                           >  2:3081665/1‑148 (MQ=255)

                                                                                                                     ttCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTg                           <  1:404995/148‑1 (MQ=255)

                                                                                                                     ttCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTg                           >  1:1939702/1‑148 (MQ=255)

                                                                                                                     ttCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTg                           <  1:2646135/148‑1 (MQ=255)

                                                                                                                       ccTACATGGGTGCAATGCGTCTGACCTAAATGGGCGTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCg                         <  2:3145992/148‑1 (MQ=255)

                                                                                                                        cTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGt                        <  2:1573558/148‑1 (MQ=255)

                                                                                                                            aTGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGaca                    >  1:1048698/1‑148 (MQ=255)

                                                                                                                             tGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGacac                   >  1:759045/1‑148 (MQ=255)

                                                                                                                              gggTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACAcc                  >  1:1136879/1‑148 (MQ=255)

                                                                                                                               ggTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACct                 >  1:52195/1‑148 (MQ=255)

                                                                                                                                    aaTGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtcttct            <  1:3428924/148‑1 (MQ=255)

                                                                                                                                     aTGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAACGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtcttctt           <  1:2994125/148‑1 (MQ=255)

                                                                                                                                     aTGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtcttctt           <  1:906959/148‑1 (MQ=255)

                                                                                                                                          tCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCAc      >  1:2679849/1‑148 (MQ=255)

                                                                                                                                          tCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCAc      >  1:2680718/1‑148 (MQ=255)

                                                                                                                                              aCCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGc  >  1:1053200/1‑148 (MQ=255)

TACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGC  >  CP009273/4097677‑4097966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`