Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,102,391 G→T 6.7% C50F (TGC→TTC)  yiiR → DUF805 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,102,3910GT 6.7% 626.9 / 3.0 215C50F (TGC→TTC) yiiRDUF805 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (105/88);  new base T (20/2);  total (125/90)
Fisher's exact test for biased strand distribution p-value = 9.65e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

TGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGATTTTACCGGGTGTCTGGCAA  >  CP009273/4102244‑4102536
                                                                                                                                                   |                                                                                                                                                 
tGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTg                                                                                                                                                   >  2:1514125/1‑148 (MQ=255)
 gACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGc                                                                                                                                                  <  2:1706771/148‑1 (MQ=255)
  aCCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGcc                                                                                                                                                 >  1:1425405/1‑148 (MQ=255)
   ccATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCt                                                                                                                                                <  1:1899942/148‑1 (MQ=255)
     aTACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTtg                                                                                                                                              >  2:1297386/1‑148 (MQ=255)
     aTACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTtg                                                                                                                                              >  1:1297723/1‑148 (MQ=255)
           cAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGtgct                                                                                                                                        <  1:3080594/148‑1 (MQ=255)
                 ttATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGctct                                                                                                                                  >  2:822232/1‑148 (MQ=255)
                    ttCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGc                                                                                                                               >  1:2735152/1‑148 (MQ=255)
                      ctcATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCa                                                                                                                             >  2:2796185/1‑148 (MQ=255)
                          tttAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAg                                                                                                                         >  1:451811/1‑148 (MQ=255)
                                 ggCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGt                                                                                                                  >  2:223601/1‑148 (MQ=255)
                                 ggCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGt                                                                                                                  >  1:3365854/1‑148 (MQ=255)
                                  gCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTTGCGTGTGTGGTTTGTCTTGCCAACACAGGCAGTa                                                                                                                 >  1:15482/1‑148 (MQ=255)
                                   cGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTaa                                                                                                                <  1:567359/148‑1 (MQ=255)
                                    gTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAAc                                                                                                               <  2:1289215/148‑1 (MQ=255)
                                     tATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACt                                                                                                              <  1:1005896/148‑1 (MQ=255)
                                      aTTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTg                                                                                                             >  1:3391641/1‑148 (MQ=255)
                                      aTTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTg                                                                                                             >  1:1653942/1‑148 (MQ=255)
                                      aTTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTg                                                                                                             <  1:244394/148‑1 (MQ=255)
                                       ttGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGt                                                                                                            <  2:44281/148‑1 (MQ=255)
                                        tGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGtt                                                                                                           <  1:3160237/148‑1 (MQ=255)
                                          gACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTaa                                                                                                         <  2:285091/148‑1 (MQ=255)
                                          gACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTaa                                                                                                         <  1:3490505/148‑1 (MQ=255)
                                           aCGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAg                                                                                                        <  2:2331715/148‑1 (MQ=255)
                                            cGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAgc                                                                                                       >  2:2191982/1‑148 (MQ=255)
                                              ccGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAgcgc                                                                                                     >  2:1892405/1‑148 (MQ=255)
                                              ccGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAgcgc                                                                                                     >  2:1280676/1‑148 (MQ=255)
                                               cGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACACCGGCAGTAACTGTTAAGCGCt                                                                                                    >  1:1544977/1‑148 (MQ=255)
                                                   aTTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCa                                                                                                <  1:228697/148‑1 (MQ=255)
                                                   aTTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCa                                                                                                >  2:2438578/1‑148 (MQ=255)
                                                   aTTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCa                                                                                                <  2:571654/148‑1 (MQ=255)
                                                      tCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGa                                                                                             <  2:1275376/148‑1 (MQ=255)
                                                       cTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGAt                                                                                            >  2:2133396/1‑148 (MQ=255)
                                                       cTGGATGTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGATTTTCTCACTGGCGCGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGTCCAACAGCGTCAGTAACTGTTAAGCGCATGCaatat                                                                                            >  2:2761464/1‑144 (MQ=255)
                                                          gATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATcgc                                                                                         >  1:1982790/1‑148 (MQ=255)
                                                           aTTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATcgcg                                                                                        >  2:1461215/1‑148 (MQ=255)
                                                             ttGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCggg                                                                                      <  2:2098594/148‑1 (MQ=255)
                                                                gATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGt                                                                                   >  2:3127727/1‑148 (MQ=255)
                                                                 aTAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTTCTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGtt                                                                                  >  1:1163048/1‑148 (MQ=255)
                                                                 aTAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCTCTTGCATGATCGCGGGCGtt                                                                                  >  1:791042/1‑148 (MQ=255)
                                                                  tAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTc                                                                                 <  1:2912956/148‑1 (MQ=255)
                                                                  tAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTc                                                                                 <  1:2556376/148‑1 (MQ=255)
                                                                     gCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGGCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAATCGCATGCATGATCGCGGGCGTTCCgg                                                                              >  1:1307187/1‑148 (MQ=255)
                                                                     gCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTTTGTGCTTGCTCTTGCCAACAGCGGCAGTAACTGTTAACCGCGTGCATGATAGCGGGCGTTCCgg                                                                              >  1:1339878/1‑148 (MQ=255)
                                                                         gtgGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATAATCAGACCGCGGCGTTTTGCCATGTGTGCTTGCTCTGTCCAACAGCGGCAGTAACTGTTAAGCGCGTTCATGATCGCGGGCGTTCCGGCGCa                                                                          >  2:1621236/1‑148 (MQ=255)
                                                                          tgGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTTGCTTGTGTTGTTTCTCTGGCCAACAGCGGCCGTAACTGTTAATCGCTTTCATGATAGCGGGCGTTCCGGCGCAt                                                                         >  1:620417/1‑148 (MQ=255)
                                                                          tgGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTTGCGTGTGTGGTTTGTCTGGCCAACAGCGGCAGTAACTGTTAATCGCTTTCATGATCGCGGGCGGTCCGGCGCaa                                                                         >  1:620402/1‑147 (MQ=255)
                                                                             ttCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGGTTGCGCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGGGGTCCGgcgccgggg                                                                      >  1:1795261/1‑143 (MQ=255)
                                                                                   ggCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTc                                                                >  2:3215604/1‑148 (MQ=255)
                                                                                     cATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCtg                                                              <  2:2357090/148‑1 (MQ=255)
                                                                                         cTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGTCAAACAGCGGCAGTAACTGTTAAGCGCTTTCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCtgatga                                                          <  1:2379606/148‑1 (MQ=255)
                                                                                         cTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCtgatga                                                          <  1:3319719/148‑1 (MQ=255)
                                                                                         cTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCGTGCTCTGGCCAACAGCGGCAGTCACTGTTAAGCGCTTGCATTATCGCGGGCGTTCCGGCGCCTGGGCATTTCtgatga                                                          >  2:117976/1‑148 (MQ=255)
                                                                                          tGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTgatgat                                                         <  1:2022648/148‑1 (MQ=255)
                                                                                           ggTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGAtt                                                        >  1:1753988/1‑148 (MQ=255)
                                                                                           ggTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTGTTTCCGTGTGTGCTTGCTCTGGCCCAAAGCGGCCGTAACAGTTAAGCGCTTGCATGTTCGCGGGCGGTCCGGCGCATGGGCCTTTCTGATGAtt                                                        >  1:1217480/1‑148 (MQ=255)
                                                                                            gTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATtg                                                       <  2:1523706/148‑1 (MQ=255)
                                                                                             tGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCGTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATtgt                                                      >  1:3365508/1‑148 (MQ=255)
                                                                                               cTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTTGCTTTTGTGCTTGCTCTGGCCCACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTATGATGATTGTgg                                                    >  1:1274438/1‑148 (MQ=255)
                                                                                                 tttCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCg                                                  >  2:703508/1‑148 (MQ=255)
                                                                                                  ttCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGt                                                 <  1:1544591/148‑1 (MQ=255)
                                                                                                   tctcACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTg                                                <  1:1280712/148‑1 (MQ=255)
                                                                                                       aCTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGAtg                                            <  2:2230241/148‑1 (MQ=255)
                                                                                                       aCTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGAtg                                            <  2:2898355/148‑1 (MQ=255)
                                                                                                           gCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTTCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGAtgctgc                                        <  2:272954/148‑1 (MQ=255)
                                                                                                           gCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGAtgctgc                                        <  1:1514125/148‑1 (MQ=255)
                                                                                                           gCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGAtgctgc                                        <  2:49361/148‑1 (MQ=255)
                                                                                                             gggTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGctgctg                                      <  1:296457/148‑1 (MQ=255)
                                                                                                              ggTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTgg                                     <  2:3479286/148‑1 (MQ=255)
                                                                                                               gTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTTCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACAGTTAATCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGc                                    >  1:832598/1‑148 (MQ=255)
                                                                                                               gTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTTCCGTGTGTGCTTGCTCTGGCCAACAGCCGCAGTAACTGTTAAGCGCTTGCATTATCGCGGGCGTTCCGGCGCATGGGCATTTATGATTATTGTGGCGTGGATGCTGGTGGc                                    >  1:997497/1‑148 (MQ=255)
                                                                                                                tAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCg                                   >  2:1957275/1‑148 (MQ=255)
                                                                                                                tAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCg                                   >  2:411857/1‑148 (MQ=255)
                                                                                                                  aGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCggg                                 <  1:2121620/148‑1 (MQ=255)
                                                                                                                  aGAATCTACTCGATATTCAGACCGCGGCGTGTTGGCGTGTGTGCTTTCTCTGGCCAACACCGGCACGAACAGTTAAGCGCTTTCATGATCGCGGGCGTTCCCGCGCAAGGGCAGTTATGATGATTGTGGCGTGGATGCTGCTGGCggg                                 >  1:2084863/1‑148 (MQ=255)
                                                                                                                    aaTCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCCGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTATGATGATTGTGGCGTGGATGCTGCTGGCGGGTa                               >  1:3408517/1‑148 (MQ=255)
                                                                                                                     aTCTACTCGATATTCAGACCGCGGCGTGTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTaa                              >  1:3156255/1‑148 (MQ=255)
                                                                                                                      tCTACTCGATATTCAGACCGCGGCGTTTTTCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACAGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAAc                             >  1:2604606/1‑148 (MQ=255)
                                                                                                                          cTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTggg                         >  1:1449515/1‑148 (MQ=255)
                                                                                                                           tCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAATCGCTTGCATGATAGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGTTGCTGCTGGCGGGTAACTGGGc                        >  1:459236/1‑148 (MQ=255)
                                                                                                                              atatTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGAt                     <  2:712650/148‑1 (MQ=255)
                                                                                                                               tatTCAGACCGCGGCGTTTTGCCTTGTGTTCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTTCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATTCTGCTGGCGGGTAACTGGGCGAtt                    >  2:618225/1‑148 (MQ=255)
                                                                                                                               tatTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTTCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGAtt                    >  2:1010591/1‑148 (MQ=255)
                                                                                                                                 ttCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGAtttt                  >  2:1354311/1‑148 (MQ=255)
                                                                                                                                 ttCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGAtttt                  >  2:2067149/1‑148 (MQ=255)
                                                                                                                                 ttCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCGGGCGGGTAACTGGGCGAtttt                  >  2:320418/1‑148 (MQ=255)
                                                                                                                                 ttCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGAACGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGAtttt                  >  2:317311/1‑148 (MQ=255)
                                                                                                                                  tCAGACCGCGGCGTTTTTCCTTGTGTGCTTGCTCTGGCCAACACCGGCAGTAACAGTTAAGCGCTTTCATGATAGCGGGCGTTCCGGCGCATGGGCATTTATGATGATTGTGGCGTGGATGCTGCTGGCGGGGAACAGGGCGATTTTa                 >  1:1044642/1‑148 (MQ=255)
                                                                                                                                  tCAGACCGCGGAGTTTTGCATTGTGTGATTGCTCTGGCCACCAGCGGCAGTAACTGTTAAGCTCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTTGGCGATTTTa                 <  1:1140833/148‑1 (MQ=255)
                                                                                                                                    aGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACGGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGATTTTAcc               >  2:927995/1‑148 (MQ=255)
                                                                                                                                         gcggcgTTGTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGATTTTACCGGgtg          <  2:3099987/148‑1 (MQ=255)
                                                                                                                                          cggcgTTTTTCCTTGTGTGCTTTCTCTGGCCAAAAGCGGCAGTAAATGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGATTTTACCGGgtgt         >  2:1534481/1‑148 (MQ=255)
                                                                                                                                           ggcgTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGATTTTACCGGGTGTc        <  1:2240631/148‑1 (MQ=255)
                                                                                                                                            gcgTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGATTTTACCGGGTGTCt       <  2:1163049/148‑1 (MQ=255)
                                                                                                                                            gcgTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGATTTTACCGGGTGTCt       <  2:852434/148‑1 (MQ=255)
                                                                                                                                               ttttGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGATTTTACCGGGTGTCTGGc    >  1:2339374/1‑148 (MQ=255)
                                                                                                                                                tttGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGATTTTACCGGGTGTCTGGCa   >  2:2267118/1‑148 (MQ=255)
                                                                                                                                                tttGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGATTTTACCGGGTGTCTGGCa   >  1:2810011/1‑148 (MQ=255)
                                                                                                                                                 ttGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGATTTTACCGGGTGTCTGGCaa  <  1:2749813/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACTGGGCGATTTTACCGGGTGTCTGGCAA  >  CP009273/4102244‑4102536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑