Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,150,054 G→T 100% S222Y (TCT→TAT)  sthA ← pyridine nucleotide transhydrogenase, soluble

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,150,0540GT99.5% 686.4 / ‑4.3 208S222Y (TCT→TAT) sthApyridine nucleotide transhydrogenase, soluble
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (111/96);  total (112/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 214 total aligned reads displayed.

ACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTG  >  CP009273/4149909‑4150199
                                                                                                                                                 |                                                                                                                                                 
aCCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATaa                                                                                                                                                 <  2:316501/148‑1 (MQ=255)
aCCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATaa                                                                                                                                                 <  1:2921934/148‑1 (MQ=255)
   gTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCt                                                                                                                                              >  2:3211307/1‑148 (MQ=255)
     tGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGa                                                                                                                                            >  2:1624654/1‑148 (MQ=255)
     tGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGa                                                                                                                                            >  1:332502/1‑148 (MQ=255)
      ggCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGAc                                                                                                                                           <  2:1397250/148‑1 (MQ=255)
      ggCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGAc                                                                                                                                           >  2:1210503/1‑148 (MQ=255)
        cATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACAt                                                                                                                                         <  1:2716119/148‑1 (MQ=255)
            gagCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATctct                                                                                                                                     <  1:158779/148‑1 (MQ=255)
             agCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCtt                                                                                                                                    >  2:652744/1‑148 (MQ=255)
              gcaggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTg                                                                                                                                   >  2:816535/1‑148 (MQ=255)
              gcaggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTg                                                                                                                                   <  1:2062030/148‑1 (MQ=255)
               caggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGa                                                                                                                                  >  2:1746794/1‑148 (MQ=255)
               caggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGa                                                                                                                                  <  1:675921/148‑1 (MQ=255)
                aggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGAt                                                                                                                                 <  2:4242/148‑1 (MQ=255)
                  gcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCCTCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCg                                                                                                                               <  2:2159734/148‑1 (MQ=255)
                  gcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCg                                                                                                                               >  1:2264249/1‑148 (MQ=255)
                      tCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGaa                                                                                                                           >  2:408675/1‑148 (MQ=255)
                       cAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGaaa                                                                                                                          <  2:3125419/148‑1 (MQ=255)
                         gCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATg                                                                                                                        >  2:2442263/1‑148 (MQ=255)
                           tttCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGcc                                                                                                                      <  2:2387300/148‑1 (MQ=255)
                           tttCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGcc                                                                                                                      <  1:2911353/148‑1 (MQ=255)
                              cAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCcagc                                                                                                                   >  1:3383634/1‑148 (MQ=255)
                              cAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCcagc                                                                                                                   <  2:3504891/148‑1 (MQ=255)
                              cAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCcagc                                                                                                                   >  1:46260/1‑148 (MQ=255)
                                 ttttttACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAgg                                                                                                                >  1:2949096/1‑148 (MQ=255)
                                    tttACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGa                                                                                                             <  1:1772096/148‑1 (MQ=255)
                                    tttACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGa                                                                                                             <  1:2038278/148‑1 (MQ=255)
                                     ttACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGAt                                                                                                            <  2:356327/148‑1 (MQ=255)
                                       aCCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATcg                                                                                                          >  2:3122008/1‑148 (MQ=255)
                                       aCCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATcg                                                                                                          >  2:167932/1‑148 (MQ=255)
                                          cGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCgg                                                                                                       <  1:1278877/148‑1 (MQ=255)
                                            acgtCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgt                                                                                                     <  2:2043654/145‑1 (MQ=255)
                                             cTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgtg                                                                                                    <  2:197088/148‑1 (MQ=255)
                                             cTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgtg                                                                                                    >  1:2969611/1‑148 (MQ=255)
                                              ttCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgtgt                                                                                                   >  1:646697/1‑148 (MQ=255)
                                              ttCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgtgt                                                                                                   >  1:3487094/1‑148 (MQ=255)
                                               tCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGtt                                                                                                  >  1:1998984/1‑148 (MQ=255)
                                                  gATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGAt                                                                                               >  1:2025892/1‑148 (MQ=255)
                                                   aTGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATc                                                                                              >  2:2130815/1‑148 (MQ=255)
                                                     gCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGACGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAg                                                                                            >  2:708396/1‑148 (MQ=255)
                                                     gCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAg                                                                                            <  1:1800949/148‑1 (MQ=255)
                                                       aTGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCCGGCGATCGCGGGTGTTGATCAGAt                                                                                          <  1:2419958/148‑1 (MQ=255)
                                                            cacaCCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACt                                                                                     <  1:2442263/148‑1 (MQ=255)
                                                             acacCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACtt                                                                                    <  2:3467122/148‑1 (MQ=255)
                                                              cacCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACttt                                                                                   >  1:1481660/1‑148 (MQ=255)
                                                                  aTCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACa                                                                               <  2:870346/148‑1 (MQ=255)
                                                                     gTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATcc                                                                            <  2:592290/148‑1 (MQ=255)
                                                                      tCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCa                                                                           <  2:313476/148‑1 (MQ=255)
                                                                       cacaGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCAt                                                                          >  2:2912095/1‑148 (MQ=255)
                                                                       cacaGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCAt                                                                          >  2:274545/1‑148 (MQ=255)
                                                                                 gATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAg                                                                <  2:2867297/148‑1 (MQ=255)
                                                                                  aTCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGa                                                               <  2:2177864/148‑1 (MQ=255)
                                                                                   tcttctCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGAt                                                              >  2:2640089/1‑148 (MQ=255)
                                                                                    cttctCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATc                                                             >  1:156474/1‑148 (MQ=255)
                                                                                         cGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGc                                                        >  2:1175595/1‑148 (MQ=255)
                                                                                           tACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCat                                                      <  2:3059028/148‑1 (MQ=255)
                                                                                            aCTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCata                                                     >  1:313137/1‑148 (MQ=255)
                                                                                            aCTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCata                                                     >  1:2734189/1‑148 (MQ=255)
                                                                                              tctTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATAtt                                                   >  1:1966729/1‑148 (MQ=255)
                                                                                              tctTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATAtt                                                   >  2:3257227/1‑148 (MQ=255)
                                                                                               ctTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTc                                                  >  1:3454677/1‑148 (MQ=255)
                                                                                               ctTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTc                                                  <  1:312284/148‑1 (MQ=255)
                                                                                                ttCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTca                                                 <  2:332502/148‑1 (MQ=255)
                                                                                                ttCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTca                                                 >  1:131834/1‑148 (MQ=255)
                                                                                                ttCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTca                                                 >  1:132853/1‑148 (MQ=255)
                                                                                                 tCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTcac                                                >  1:709320/1‑148 (MQ=255)
                                                                                                  cGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTcaca                                               >  2:2778009/1‑148 (MQ=255)
                                                                                                  cGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTcaca                                               <  1:950466/148‑1 (MQ=255)
                                                                                                    ttGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGc                                             >  2:2085209/1‑148 (MQ=255)
                                                                                                     ttttaCGTATCAATACTCCACTGTTCCAGATGTGATTGGAGATATAATCTGACATCTCTTGATCGATAAATGCCAGCTGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGcc                                            <  1:3440094/144‑1 (MQ=255)
                                                                                                     tgtgACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGcc                                            <  1:915421/148‑1 (MQ=255)
                                                                                                        gACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGAt                                         >  2:1121153/1‑148 (MQ=255)
                                                                                                         aCGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATc                                        >  1:1250158/1‑148 (MQ=255)
                                                                                                           gAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCAc                                      <  1:2561403/148‑1 (MQ=255)
                                                                                                            aaTCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACt                                     <  2:129898/148‑1 (MQ=255)
                                                                                                              tCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAg                                              <  2:1864984/137‑1 (MQ=255)
                                                                                                              tCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAg                                              >  1:1864984/1‑137 (MQ=255)
                                                                                                              tCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTcc                                   >  1:3235702/1‑148 (MQ=255)
                                                                                                              tCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTcc                                   >  1:2994657/1‑148 (MQ=255)
                                                                                                                aCTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAg                                 >  2:3400272/1‑148 (MQ=255)
                                                                                                                 cTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGc                                >  1:435813/1‑148 (MQ=255)
                                                                                                                   aCGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                              >  2:787390/1‑148 (MQ=255)
                                                                                                                   aCGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                              >  1:1984423/1‑148 (MQ=255)
                                                                                                                    cGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAcc                             >  1:86700/1‑148 (MQ=255)
                                                                                                                    cGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAcc                             <  1:2455887/148‑1 (MQ=255)
                                                                                                                      ccACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCAt                           <  1:434128/148‑1 (MQ=255)
                                                                                                                           gTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATa                      <  1:1423870/148‑1 (MQ=255)
                                                                                                                             tCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAg                    >  2:769913/1‑148 (MQ=255)
                                                                                                                                 gAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACa                <  1:167932/148‑1 (MQ=255)
                                                                                                                                   aGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATg              <  2:1250158/148‑1 (MQ=255)
                                                                                                                                   aGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATg              <  1:2337049/148‑1 (MQ=255)
                                                                                                                                       aTAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGgcgc          >  2:2698843/1‑148 (MQ=255)
                                                                                                                                       aTAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGgcgc          >  1:593571/1‑148 (MQ=255)
                                                                                                                                         aGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCgg        <  1:1210503/148‑1 (MQ=255)
                                                                                                                                            agagataATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTc     >  1:3220282/1‑148 (MQ=255)
                                                                                                                                            agagataATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTc     >  1:1570258/1‑148 (MQ=255)
                                                                                                                                             gagataATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTCCAGCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATACGTACCGGGCGCGGTTCg    <  2:2282971/148‑1 (MQ=255)
                                                                                                                                              agataATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTctt   >  1:1040755/1‑146 (MQ=255)
                                                                                                                                               gagaATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCgtg  <  2:501667/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
ACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTG  >  CP009273/4149909‑4150199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑