BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,207,552	G→T	100%	M172I (ATG→ATT)	aceA →	isocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,207,552	0	G	T	100.0%	555.9 / NA	167	M172I (ATG→ATT)	aceA	isocitrate lyase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (91/76); total (91/76)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 173 total aligned reads displayed.

CAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCT  >  CP009273/4207408‑4207696

caacaaCACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTaaa                                                                                                                                               >  2:2714310/1‑148 (MQ=255)

caacaaCACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTaaa                                                                                                                                               >  2:1341660/1‑148 (MQ=255)

 aacaacACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAg                                                                                                                                              <  2:3494938/148‑1 (MQ=255)

   caacaCCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCg                                                                                                                                            <  1:113696/148‑1 (MQ=255)

   caacaCCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCg                                                                                                                                            <  2:3425489/148‑1 (MQ=255)

    aacaCCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCga                                                                                                                                           <  2:1528294/148‑1 (MQ=255)

    aacaCCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCga                                                                                                                                           <  1:3154022/148‑1 (MQ=255)

     acacCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCgat                                                                                                                                          >  1:1022407/1‑148 (MQ=255)

      cacCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCgatg                                                                                                                                         <  1:2151074/148‑1 (MQ=255)

       acCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCgatga                                                                                                                                        <  2:3118900/148‑1 (MQ=255)

           tCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGa                                                                                                                                    >  1:1180164/1‑148 (MQ=255)

                 gtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCgg                                                                                                                              <  1:3143902/148‑1 (MQ=255)

                  tGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGt                                                                                                                             <  1:3162663/148‑1 (MQ=255)

                  tGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGt                                                                                                                             >  1:940727/1‑148 (MQ=255)

                   gCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTg                                                                                                                            >  1:1382207/1‑148 (MQ=255)

                      gATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAg                                                                                                                         >  2:2141340/1‑148 (MQ=255)

                      gATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAg                                                                                                                         >  2:3373581/1‑148 (MQ=255)

                        tCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCg                                                                                                                       >  1:820067/1‑148 (MQ=255)

                        tCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGACGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCg                                                                                                                       >  2:2075009/1‑148 (MQ=255)

                         cAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGTGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCgg                                                                                                                      >  1:1503955/1‑148 (MQ=255)

                         cAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGTGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCgg                                                                                                                      >  1:1504009/1‑148 (MQ=255)

                           gATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCa                                                                                                                    >  1:2574661/1‑148 (MQ=255)

                             tCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGt                                                                                                                  >  1:95678/1‑148 (MQ=255)

                               cAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTc                                                                                                                >  1:1830019/1‑148 (MQ=255)

                               cAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGAATAAAGCGATGATTGAAGCAGGTGCAGCGGCAGTTc                                                                                                                >  1:1872158/1‑148 (MQ=255)

                                 aTGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCAc                                                                                                              >  1:2894019/1‑148 (MQ=255)

                                   ggTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACtt                                                                                                            <  1:2257949/148‑1 (MQ=255)

                                   ggTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACtt                                                                                                            <  2:466703/148‑1 (MQ=255)

                                   ggTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGACGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGACTTTGAACTGTTTAAAGCTATGATTGAAGACGGTGCAGCGGCAGTTCACtt                                                                                                            <  1:491952/148‑1 (MQ=255)

                                    gTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGAGTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTc                                                                                                           >  2:37736/1‑148 (MQ=255)

                                     tCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCg                                                                                                          >  2:783894/1‑148 (MQ=255)

                                     tCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCg                                                                                                          >  1:691257/1‑148 (MQ=255)

                                     tCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCg                                                                                                          <  1:1757189/148‑1 (MQ=255)

                                     tCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCg                                                                                                          <  1:1757351/148‑1 (MQ=255)

                                     tCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCg                                                                                                          >  1:1987479/1‑148 (MQ=255)

                                      ccGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGa                                                                                                         <  1:865025/148‑1 (MQ=255)

                                          gggCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGAt                                                                                                     <  1:1236597/148‑1 (MQ=255)

                                             cATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAg                                                                                                  <  1:227031/148‑1 (MQ=255)

                                             cATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAg                                                                                                  <  2:109559/148‑1 (MQ=255)

                                             cATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAg                                                                                                  <  2:177820/148‑1 (MQ=255)

                                             cATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAg                                                                                                  <  2:542208/148‑1 (MQ=255)

                                             cATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAg                                                                                                  <  2:1166407/148‑1 (MQ=255)

                                              aTTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGc                                                                                                 <  2:1650259/148‑1 (MQ=255)

                                               ttGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCt                                                                                                >  1:1228915/1‑148 (MQ=255)

                                                 gAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTgg                                                                                              <  2:2818373/148‑1 (MQ=255)

                                                  aGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGc                                                                                             >  2:3110926/1‑148 (MQ=255)

                                                    ccGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGt                                                                                           <  1:2078704/148‑1 (MQ=255)

                                                    ccGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGt                                                                                           <  1:2078800/148‑1 (MQ=255)

                                                       ggCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAg                                                                                        <  2:1020372/148‑1 (MQ=255)

                                                        gCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGt                                                                                       >  2:2659412/1‑148 (MQ=255)

                                                            tCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTgaag                                                                                   >  2:1721721/1‑148 (MQ=255)

                                                             ccGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTgaaga                                                                                  >  2:1000026/1‑148 (MQ=255)

                                                              cgcgCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTgaagaa                                                                                 <  1:753427/148‑1 (MQ=255)

                                                               gcgcTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGaaa                                                                                <  2:438240/148‑1 (MQ=255)

                                                                  cTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGc                                                                             >  1:1017617/1‑148 (MQ=255)

                                                                  cTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGc                                                                             >  2:3427757/1‑148 (MQ=255)

                                                                   tATGTATATTACTTCCTGCCTATCGTTGCCGATGCGGTAGCCGGTTTTGGCGGTGTCCTGACTGCCTTTGTACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCg                                                                            <  2:3166151/148‑1 (MQ=255)

                                                                     tGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGt                                                                          >  2:369431/1‑148 (MQ=255)

                                                                     tGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGt                                                                          >  2:369597/1‑148 (MQ=255)

                                                                           ttACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCTGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATg                                                                    >  2:103349/1‑148 (MQ=255)

                                                                            tACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATgg                                                                   >  2:1798022/1‑148 (MQ=255)

                                                                              cTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGggc                                                                 <  1:2452374/148‑1 (MQ=255)

                                                                                   tGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCaa                                                            >  1:1163418/1‑148 (MQ=255)

                                                                                    gCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCaaa                                                           >  2:641564/1‑148 (MQ=255)

                                                                                    gCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCaaa                                                           <  2:1901751/148‑1 (MQ=255)

                                                                                     ccGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAg                                                          >  2:2226624/1‑148 (MQ=255)

                                                                                       gATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGtt                                                        <  1:865578/148‑1 (MQ=255)

                                                                                         tCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGtttt                                                      <  2:1382207/148‑1 (MQ=255)

                                                                                          cGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTa                                                     >  2:3272544/1‑148 (MQ=255)

                                                                                          cGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTa                                                     <  2:1849630/148‑1 (MQ=255)

                                                                                                cGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCa                                               <  1:2057081/148‑1 (MQ=255)

                                                                                                       gAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAgg                                        >  1:1978471/1‑148 (MQ=255)

                                                                                                        aaGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGa                                       >  1:1479912/1‑148 (MQ=255)

                                                                                                          gCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAg                                     >  1:1278976/1‑148 (MQ=255)

                                                                                                          gCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAg                                     <  1:2009543/148‑1 (MQ=255)

                                                                                                          gCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAg                                     >  1:589871/1‑148 (MQ=255)

                                                                                                           ccGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGc                                    >  2:2246401/1‑148 (MQ=255)

                                                                                                           ccGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGc                                    <  2:2574661/148‑1 (MQ=255)

                                                                                                               ttttGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTAtt                                >  2:2942663/1‑148 (MQ=255)

                                                                                                               ttttGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTAtt                                >  2:684953/1‑148 (MQ=255)

                                                                                                                    gCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGaa                           >  1:3054491/1‑148 (MQ=255)

                                                                                                                       gtgtCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACt                        <  2:1229034/148‑1 (MQ=255)

                                                                                                                          tCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGt                     >  1:2698084/1‑148 (MQ=255)

                                                                                                                           ccTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTc                    >  2:2556232/1‑148 (MQ=255)

                                                                                                                           ccTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTc                    >  2:2556224/1‑148 (MQ=255)

                                                                                                                            cTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTcg                   >  2:842178/1‑148 (MQ=255)

                                                                                                                              gAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTcgcg                 >  1:896418/1‑148 (MQ=255)

                                                                                                                               aaTGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCgcgg                >  2:46318/1‑148 (MQ=255)

                                                                                                                                 tGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCgcggcg              >  1:2137409/1‑148 (MQ=255)

                                                                                                                                 tGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCgcggcg              >  2:2853395/1‑148 (MQ=255)

                                                                                                                                 tGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGCTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCgcggcg              >  2:1620762/1‑148 (MQ=255)

                                                                                                                                   ccTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGcgcg            <  2:2698084/148‑1 (MQ=255)

                                                                                                                                   ccTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGcgcg            >  2:3403438/1‑148 (MQ=255)

                                                                                                                                   ccTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGcgcg            >  1:2293042/1‑148 (MQ=255)

                                                                                                                                    cTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGt           <  1:1721721/148‑1 (MQ=255)

                                                                                                                                    cTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGt           <  2:961828/148‑1 (MQ=255)

                                                                                                                                         aaCTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGc      >  1:1302257/1‑148 (MQ=255)

                                                                                                                                            tgattaAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGc   >  1:505516/1‑148 (MQ=255)

                                                                                                                                             gattaAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCt  >  2:1346410/1‑148 (MQ=255)

                                                                                                                                             gattaAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCt  <  1:856003/148‑1 (MQ=255)

CAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCT  >  CP009273/4207408‑4207696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`