Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,210,753 C→A 100% E555* (GAA→TAA)  arpA ← ankyrin repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,210,7530CA99.1% 680.2 / ‑3.3 207E555* (GAA→TAA) arpAankyrin repeat protein
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (105/100);  total (105/102)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 207 total aligned reads displayed.

GTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATG  >  CP009273/4210608‑4210896
                                                                                                                                                 |                                                                                                                                               
gTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACt                                                                                                                                               <  1:3133138/148‑1 (MQ=255)
gTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACt                                                                                                                                               <  1:31829/148‑1 (MQ=255)
  aTTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTgg                                                                                                                                             >  1:2586441/1‑148 (MQ=255)
   ttAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTggg                                                                                                                                            <  1:2652715/148‑1 (MQ=255)
    tAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGc                                                                                                                                           <  2:2066667/148‑1 (MQ=255)
     aaaCAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCt                                                                                                                                          <  2:1573898/148‑1 (MQ=255)
        cAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAgg                                                                                                                                       >  2:1214866/1‑148 (MQ=255)
         agagTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGc                                                                                                                                      <  1:138861/148‑1 (MQ=255)
           agTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTg                                                                                                                                    >  1:1281605/1‑148 (MQ=255)
            gTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTgg                                                                                                                                   >  2:2053915/1‑148 (MQ=255)
             tGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTggg                                                                                                                                  >  1:164772/1‑148 (MQ=255)
               ggTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCAGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCa                                                                                                                                >  1:3448574/1‑148 (MQ=255)
                gTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCaa                                                                                                                               <  2:2390392/148‑1 (MQ=255)
                    tGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCt                                                                                                                           <  1:2760259/148‑1 (MQ=255)
                     gCGTTAAAAATAGTATTTATACCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCTGATGCTGTAATGTTAATTTACTGGGCTAGGCTGGGCAATGCtt                                                                                                                          <  2:3400090/148‑1 (MQ=255)
                     gCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCtt                                                                                                                          <  2:1660542/148‑1 (MQ=255)
                        ttAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCaa                                                                                                                       >  1:913989/1‑148 (MQ=255)
                           aaaaTAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGaa                                                                                                                    >  1:1015825/1‑148 (MQ=255)
                           aaaaTAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGaa                                                                                                                    <  2:353289/148‑1 (MQ=255)
                             aaTAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATa                                                                                                                  >  1:995631/1‑148 (MQ=255)
                             aaTAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATa                                                                                                                  >  1:3112841/1‑148 (MQ=255)
                                aGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACt                                                                                                               <  1:1263140/148‑1 (MQ=255)
                                 gTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACtt                                                                                                              >  1:1616320/1‑148 (MQ=255)
                                   aTTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTc                                                                                                            <  2:2057292/148‑1 (MQ=255)
                                     taataaCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCAc                                                                                                          >  2:1717173/1‑148 (MQ=255)
                                     taataaCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCAc                                                                                                          >  2:1829360/1‑148 (MQ=255)
                                     taataaCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCAc                                                                                                          >  1:2766592/1‑148 (MQ=255)
                                      aataaCGTTCATGTGTCCGCTCTGCATGGCCATAAACAAACCCGTGTCGCGCGCATGACTTTTAGCGGTCAGAAGATGTACAATATCGGTAGCTGAAATGTTAATTGACTGGGCTAGGCTGTGCAATGCTTCAAGAATAACTTTCACg                                                                                                         <  1:2822641/148‑1 (MQ=255)
                                      aataaCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACg                                                                                                         >  2:3370405/1‑148 (MQ=255)
                                         aaCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGata                                                                                                      >  1:180637/1‑148 (MQ=255)
                                           cGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATc                                                                                                    >  2:1208294/1‑148 (MQ=255)
                                             ttCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGc                                                                                                  <  1:176306/148‑1 (MQ=255)
                                             ttCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCAAGATATCga                                                                                                  >  1:2019379/1‑147 (MQ=255)
                                              tCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAGTGCTTCAAGAATAACTTTCACGATATCGCt                                                                                                 <  2:1151829/148‑1 (MQ=255)
                                                aTGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTAt                                                                                               >  2:2688463/1‑148 (MQ=255)
                                                aTGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTCAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTAt                                                                                               >  2:2692035/1‑148 (MQ=255)
                                                  gtgtCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGt                                                                                             <  1:2290045/148‑1 (MQ=255)
                                                  gtgtCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGt                                                                                             >  2:1518479/1‑148 (MQ=255)
                                                    gtCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTcc                                                                                           <  2:2514783/148‑1 (MQ=255)
                                                    gtCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTcc                                                                                           <  2:2111459/148‑1 (MQ=255)
                                                    gtCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTcc                                                                                           <  1:2061245/148‑1 (MQ=255)
                                                      ccGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCAt                                                                                         >  2:3390288/1‑148 (MQ=255)
                                                      ccGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCAt                                                                                         >  2:2420029/1‑148 (MQ=255)
                                                           cTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGc                                                                                    <  1:1417406/148‑1 (MQ=255)
                                                                  gCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCa                                                                             <  2:1648383/148‑1 (MQ=255)
                                                                     aTAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGat                                                                          <  1:3245790/148‑1 (MQ=255)
                                                                      tAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGata                                                                         <  2:555762/148‑1 (MQ=255)
                                                                      tAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGata                                                                         >  1:2460344/1‑148 (MQ=255)
                                                                            aacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTcc                                                                   <  2:3383152/148‑1 (MQ=255)
                                                                            aacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTcc                                                                   >  1:1335792/1‑148 (MQ=255)
                                                                            aacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTcc                                                                   >  1:2369269/1‑148 (MQ=255)
                                                                                cGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTggg                                                               <  2:1281605/148‑1 (MQ=255)
                                                                                   gtCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCaa                                                            <  2:3044362/148‑1 (MQ=255)
                                                                                           cAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATaaaa                                                    <  2:3449485/148‑1 (MQ=255)
                                                                                             aGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAAt                                                  >  1:1259543/1‑148 (MQ=255)
                                                                                             aGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAAt                                                  <  2:1888976/148‑1 (MQ=255)
                                                                                             aGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAAt                                                  >  2:3341778/1‑148 (MQ=255)
                                                                                              gACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATc                                                 <  2:3305978/148‑1 (MQ=255)
                                                                                               aCTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATcc                                                <  2:233573/148‑1 (MQ=255)
                                                                                               aCTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATcc                                                <  2:2788197/148‑1 (MQ=255)
                                                                                               aCTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATcc                                                <  2:2788446/148‑1 (MQ=255)
                                                                                                cTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCt                                               >  2:566044/1‑148 (MQ=255)
                                                                                                cTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCt                                               <  1:1569486/148‑1 (MQ=255)
                                                                                                cTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCt                                               <  1:2128444/148‑1 (MQ=255)
                                                                                                 ttttAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTATTTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCtt                                              <  1:1062493/148‑1 (MQ=255)
                                                                                                 ttttAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCtt                                              <  2:2388059/148‑1 (MQ=255)
                                                                                                   ttAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTg                                            <  2:1456581/148‑1 (MQ=255)
                                                                                                       cGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCtt                                        >  2:2909488/1‑148 (MQ=255)
                                                                                                              aagaTCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGgaga                                 <  2:902138/148‑1 (MQ=255)
                                                                                                              aagaTCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGgaga                                 <  2:2175611/148‑1 (MQ=255)
                                                                                                              aagaTCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGgaga                                 <  2:2256290/148‑1 (MQ=255)
                                                                                                               agaTCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGAt                                >  2:103109/1‑148 (MQ=255)
                                                                                                                gaTCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATc                               >  2:2746251/1‑148 (MQ=255)
                                                                                                                gaTCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATc                               <  2:2586441/148‑1 (MQ=255)
                                                                                                                  tCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGa                             >  2:2910629/1‑148 (MQ=255)
                                                                                                                    tACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGt                           >  2:468076/1‑148 (MQ=255)
                                                                                                                    tACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGt                           <  1:2628373/148‑1 (MQ=255)
                                                                                                                     aCAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTa                          >  2:2606366/1‑148 (MQ=255)
                                                                                                                     aCAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTa                          >  1:1841904/1‑148 (MQ=255)
                                                                                                                     aCAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTa                          <  2:2804567/148‑1 (MQ=255)
                                                                                                                       aaTATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTAcc                        <  2:121091/148‑1 (MQ=255)
                                                                                                                        atatCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCGTCAGGAGATCGAGTACCt                       >  1:1927643/1‑148 (MQ=255)
                                                                                                                          atCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGt                     >  2:2611448/1‑148 (MQ=255)
                                                                                                                           tCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGtt                    >  1:823494/1‑148 (MQ=255)
                                                                                                                             ggAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCt                  <  2:2766592/148‑1 (MQ=255)
                                                                                                                              gAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCtt                 >  1:677377/1‑148 (MQ=255)
                                                                                                                              gAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCtt                 >  1:1010627/1‑148 (MQ=255)
                                                                                                                               aaGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCttt                <  1:1208294/148‑1 (MQ=255)
                                                                                                                                aGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTa               >  2:1069654/1‑148 (MQ=255)
                                                                                                                                  cTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGt             >  2:796619/1‑148 (MQ=255)
                                                                                                                                     aaaTGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCaa          <  2:2128329/148‑1 (MQ=255)
                                                                                                                                     aaaTGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCaa          >  1:3511511/1‑148 (MQ=255)
                                                                                                                                       aTGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAAt        >  1:625676/1‑148 (MQ=255)
                                                                                                                                       aTGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAAt        >  1:625495/1‑148 (MQ=255)
                                                                                                                                        tGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATg       <  1:3393578/148‑1 (MQ=255)
                                                                                                                                        tGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATg       >  2:656049/1‑148 (MQ=255)
                                                                                                                                          ttAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGAc     <  2:3112841/148‑1 (MQ=255)
                                                                                                                                          ttAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGAc     >  2:1355132/1‑148 (MQ=255)
                                                                                                                                          ttAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGAc     <  2:1366114/148‑1 (MQ=255)
                                                                                                                                             aTTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACAtg  >  2:1557620/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATG  >  CP009273/4210608‑4210896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑