| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,214,809 | G→T | 100% | E352* (GAA→TAA) | metH → | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,214,809 | 0 | G | T | 95.6% | 577.8 / 5.7 | 221 | E352* (GAA→TAA) | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (12/2); new base T (122/85); total (134/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.20e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 221 total aligned reads displayed.
TCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATA > CP009273/4214664‑4214953 | ctcAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAg > 1:2480153/1‑148 (MQ=255) ctcAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAg > 1:2927766/1‑148 (MQ=255) cAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGAt < 2:1594675/148‑1 (MQ=255) aaTATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGaaa > 1:2430776/1‑146 (MQ=255) aaTATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATa > 2:3157706/1‑148 (MQ=255) atatCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAg < 1:1658261/148‑1 (MQ=255) gtcgGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGt < 1:3191059/148‑1 (MQ=255) gtcgGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGt < 1:3413957/148‑1 (MQ=255) ctgctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAAc > 2:3048718/1‑148 (MQ=255) ctgctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAAc > 2:1848596/1‑148 (MQ=255) ctgctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAAc > 1:356936/1‑148 (MQ=255) ctgctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGG‑AACATTGGGGAAGATAGCCTGTTTGTGAAc > 1:2655940/1‑148 (MQ=255) gctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGt < 1:1039365/148‑1 (MQ=255) gctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGGTAATATAGCCTGTTTTTGaaact > 1:783950/1‑145 (MQ=255) ctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTg > 2:3327551/1‑148 (MQ=255) gtgGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTggg < 2:2813850/148‑1 (MQ=255) tgGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGt > 2:2234757/1‑148 (MQ=255) caccacGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGaa < 2:1006710/148‑1 (MQ=255) caccacGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGaa < 1:950035/148‑1 (MQ=255) ccacGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACg > 2:2778857/1‑148 (MQ=255) acGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCa < 2:2572001/148‑1 (MQ=255) gCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCAcc > 1:3437118/1‑148 (MQ=255) acaacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAAc > 1:3094563/1‑148 (MQ=255) acaacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGG‑GACAATGGCGAATATAGCCAGTTTTTGAAAGTGGGGGAACGCACCCAc > 1:1200988/1‑148 (MQ=255) caacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACg > 1:521368/1‑148 (MQ=255) caacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACg > 1:1455975/1‑148 (MQ=255) caacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACg > 1:2933015/1‑148 (MQ=255) caacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGGGGGTGAACGCACCAACg > 1:521122/1‑148 (MQ=255) caTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCa > 1:424491/1‑148 (MQ=255) atatTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGTACGCACCAACGTCAc > 1:1025424/1‑148 (MQ=255) tatTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCAcc > 2:2901034/1‑148 (MQ=255) tatTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGCTAAGATAGCCTGTTTGTGAACGTGGGTGTAAGCACCAACGTCAcc > 1:2985517/1‑148 (MQ=255) atTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCg < 1:2638082/148‑1 (MQ=255) atTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCg > 1:2794343/1‑148 (MQ=255) tGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGt > 1:2043635/1‑148 (MQ=255) tGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGt < 2:1076000/148‑1 (MQ=255) tGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGt > 2:2009252/1‑148 (MQ=255) gCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGtt < 1:2315563/148‑1 (MQ=255) cAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTc > 2:1196068/1‑148 (MQ=255) cAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTc > 2:913888/1‑148 (MQ=255) cAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTc > 1:1669080/1‑148 (MQ=255) cGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATATCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGc > 1:3002159/1‑148 (MQ=255) ctGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTa < 2:2522151/147‑1 (MQ=255) aTGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCACACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTa < 2:3383551/148‑1 (MQ=255) aTGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTa > 2:441983/1‑148 (MQ=255) aTGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCCGCCTGGAGCCGCGG‑AAAAATGGCTAATATATCCTGTTTTTGAACGTGGGTGTAAGCACCAACCTCACCGGTTccccga > 1:1249551/1‑144 (MQ=255) gAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAAACATT‑GCGAAGATAACCATTTTTTGAACCTGGGGGAACCCACCAACGTCACCCGTTACGCTaat > 1:1556535/1‑147 (MQ=255) gAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGCTAAGATAGCCTGTTTTTGAACGTGGGTGAACGCACCAACCTCACCGGTTCCGCGAAg > 1:1382978/1‑148 (MQ=255) aGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAgcgc < 1:1934938/148‑1 (MQ=255) tAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCt > 1:2780546/1‑148 (MQ=255) aGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTg < 2:3289618/148‑1 (MQ=255) gAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGa < 1:2347595/148‑1 (MQ=255) aaGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGAt < 1:2161125/148‑1 (MQ=255) ggATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCa > 2:1252731/1‑148 (MQ=255) gATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGGTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCaa > 1:1585865/1‑148 (MQ=255) gATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCaa > 1:2605305/1‑148 (MQ=255) ttAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCGAAGTTCAAGCGCCTGATCAaag > 2:2665766/1‑148 (MQ=255) tAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAaaga < 2:1339325/148‑1 (MQ=255) tAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAaaga < 2:1338733/148‑1 (MQ=255) gcgcCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAaagaag < 2:809311/148‑1 (MQ=255) gcgcCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCGAAGTTCAAGCGCCGGATCAaagaag > 1:1589276/1‑148 (MQ=255) ccgcGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAgaga < 2:763983/148‑1 (MQ=255) ccgcGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAgaga < 2:1699543/148‑1 (MQ=255) cgcgCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGaa < 1:2028318/148‑1 (MQ=255) gcgcAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGaaa < 1:436305/148‑1 (MQ=255) gcAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATa < 2:545681/148‑1 (MQ=255) gcAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATa < 2:356936/148‑1 (MQ=255) aaaCTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACa < 1:1013029/148‑1 (MQ=255) aaaCTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACa < 2:2082067/148‑1 (MQ=255) aaCTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGCTAAGATATCCTGTTTGTGAACGGGGGTGAACGCACCAACGTCACCGGTTCCGCGAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAg > 1:1962535/1‑148 (MQ=255) aaCTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAg > 2:2904344/1‑148 (MQ=255) aCTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTTGTTGTGAACGTGGGTGTACGCACCAACGTCACCGGTTCCGCTAAGTTCAATCGCGTGATCAAAGTAGAGAAATACAGc > 2:590470/1‑148 (MQ=255) cTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTT‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCg < 1:3102685/148‑1 (MQ=255) cTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCg < 1:1850753/148‑1 (MQ=255) gAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGgcgc > 2:1248514/1‑148 (MQ=255) aTTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTgg < 2:859826/148‑1 (MQ=255) aTTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCGAAGTTCAAGCGCCTGATCAAAGAAGAGAAATAAAGCGAGGCGCTgg > 2:1509177/1‑148 (MQ=255) ttCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGCTAAGATAGCCAGTTTTTGAAAGTGGGTGAAAGCACCCACGTCACCCGTTGCGCGAATGTAAAGCGCCAGGTCAAACAAGAGTAAAACACCGAGGCGCTGGa > 1:2413949/1‑148 (MQ=255) ttCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGa > 1:3194460/1‑148 (MQ=255) ttCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGa > 1:1448928/1‑148 (MQ=255) ttCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AAAATTTGCTAAGATAGCCTGTTTTTGTACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAATCGCCGGATCAAATAAGAGAAAAACAGCGAGGCGCGGGa > 1:1363832/1‑148 (MQ=255) tCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGCTAAGATAGCCGGTTTGTTAAACTGGGTGAACGCCCCCACCTCCCCCGTTCCGCGAAGATAAAGCGCCCGATAAAATAAGAGAAAAAAACAGAGGCGCTGGAt > 1:1947530/1‑148 (MQ=255) cccGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATg < 1:256615/148‑1 (MQ=255) cGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTc > 2:2875562/1‑148 (MQ=255) cGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTc > 2:3121675/1‑148 (MQ=255) cGTAGCCTGCCGTTTGTCCGGCCATGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTTTGAACGTGGGTGAACGCACCAACGTCACCGGTTACGCTAAGTTAAAGCCCCTGATAAAAGAAGAGAAATAAAGCGAGGCGCTGGATGTc > 2:58441/1‑148 (MQ=255) ccTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTcgcgcg > 2:2175022/1‑148 (MQ=255) cGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAAc > 2:1100434/1‑148 (MQ=255) gTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACa < 2:2480153/148‑1 (MQ=255) gTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGAGAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACa < 2:476024/148‑1 (MQ=255) ccGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTAAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGa > 1:724262/1‑148 (MQ=255) tGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACgg > 2:2339936/1‑148 (MQ=255) gCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGTTGTCGCGCGTCAACAGGTGGAAAACGgcgcg > 2:587498/1‑148 (MQ=255) gCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgcgcg > 2:3076884/1‑148 (MQ=255) ccGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgcgcgc > 1:85061/1‑148 (MQ=255) cTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGa > 2:3433515/1‑148 (MQ=255) cTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGa < 1:1708017/148‑1 (MQ=255) g‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGAtt > 1:1150409/1‑148 (MQ=255) aaCATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTa < 2:1937214/148‑1 (MQ=255) tGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGat > 1:1873378/1‑148 (MQ=255) | TCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATA > CP009273/4214664‑4214953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |