Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,219,858 G→T 92.8% V71V (GTC→GTA)  pepE ← (alpha)‑aspartyl dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,219,8580GT92.8% 506.8 / 29.1 181V71V (GTC→GTA) pepE(alpha)‑aspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base G (10/3);  new base T (79/89);  total (89/92)
Fisher's exact test for biased strand distribution p-value = 4.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

CCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGCAATT  >  CP009273/4219714‑4220002
                                                                                                                                                |                                                                                                                                                
ccAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATAGCGGCAACGGGATCTACa                                                                                                                                               >  2:2552508/1‑148 (MQ=255)
 cAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACaa                                                                                                                                              <  2:2821691/148‑1 (MQ=255)
 cAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACaa                                                                                                                                              <  1:1752053/148‑1 (MQ=255)
  aGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAAc                                                                                                                                             >  2:1016404/1‑148 (MQ=255)
   gCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACg                                                                                                                                            <  1:2314502/148‑1 (MQ=255)
   gCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACg                                                                                                                                            <  2:871378/148‑1 (MQ=255)
   gCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACg                                                                                                                                            >  1:98935/1‑148 (MQ=255)
    ccAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGc                                                                                                                                           >  1:3450621/1‑148 (MQ=255)
     cAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCt                                                                                                                                          >  1:2871771/1‑148 (MQ=255)
     cAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCt                                                                                                                                          <  1:2262858/148‑1 (MQ=255)
      aaTATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTa                                                                                                                                         <  1:1749799/148‑1 (MQ=255)
       atatACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTAt                                                                                                                                        <  2:3260496/148‑1 (MQ=255)
        tataCAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATg                                                                                                                                       >  1:1486093/1‑148 (MQ=255)
         ataCAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGa                                                                                                                                      >  2:1939879/1‑148 (MQ=255)
         ataCAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGa                                                                                                                                      >  2:2745857/1‑148 (MQ=255)
              gagCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATAcc                                                                                                                                 >  1:988688/1‑148 (MQ=255)
              gagCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATAcc                                                                                                                                 >  1:993088/1‑148 (MQ=255)
                    cACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGAc                                                                                                                           <  2:3359593/148‑1 (MQ=255)
                      cGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAg                                                                                                                         <  2:1534718/148‑1 (MQ=255)
                              cACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACAccc                                                                                                                 <  2:3385503/148‑1 (MQ=255)
                               aCGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCa                                                                                                                >  2:1098765/1‑148 (MQ=255)
                                  tCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCTCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCg                                                                                                             >  1:1419374/1‑148 (MQ=255)
                                   cAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCgg                                                                                                            >  2:2237178/1‑148 (MQ=255)
                                   cAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCgg                                                                                                            >  2:2962637/1‑148 (MQ=255)
                                   cAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCgg                                                                                                            <  2:1321201/148‑1 (MQ=255)
                                     gTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAg                                                                                                          <  2:16835/148‑1 (MQ=255)
                                     gTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAg                                                                                                          >  1:879353/1‑148 (MQ=255)
                                       aaTTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCg                                                                                                        >  2:3117839/1‑148 (MQ=255)
                                       aaTTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCg                                                                                                        <  1:94895/148‑1 (MQ=255)
                                        aTTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCga                                                                                                       <  2:1201258/148‑1 (MQ=255)
                                                cagcagCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCa                                                                                               >  1:1317376/1‑148 (MQ=255)
                                                 agcagcCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAg                                                                                              <  2:1486093/148‑1 (MQ=255)
                                                 agcagcCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAg                                                                                              <  1:2938808/148‑1 (MQ=255)
                                                  gcagcCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGc                                                                                             >  1:1099993/1‑148 (MQ=255)
                                                  gcagcCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGc                                                                                             >  1:2966536/1‑148 (MQ=255)
                                                   cagcCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGcc                                                                                            >  2:598848/1‑148 (MQ=255)
                                                     gcCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGt                                                                                          >  1:2604715/1‑148 (MQ=255)
                                                        cgcgCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGtttt                                                                                       <  1:3018524/148‑1 (MQ=255)
                                                        cgcgCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGtttt                                                                                       >  2:418407/1‑148 (MQ=255)
                                                          cgcTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCg                                                                                     >  2:2088638/1‑148 (MQ=255)
                                                          cgcTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCg                                                                                     <  2:368435/148‑1 (MQ=255)
                                                           gcTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGc                                                                                    <  2:1526032/148‑1 (MQ=255)
                                                           gcTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGc                                                                                    <  1:1707871/148‑1 (MQ=255)
                                                            cTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCt                                                                                   <  1:1835751/148‑1 (MQ=255)
                                                             tCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCtg                                                                                  <  2:2714624/148‑1 (MQ=255)
                                                             tCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCtg                                                                                  <  2:540870/148‑1 (MQ=255)
                                                               gcgGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCtgtg                                                                                <  1:1378779/148‑1 (MQ=255)
                                                                 ggCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTa                                                                              <  1:3254508/148‑1 (MQ=255)
                                                                 ggCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTa                                                                              <  2:87761/148‑1 (MQ=255)
                                                                  gCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTaa                                                                             <  2:2876333/148‑1 (MQ=255)
                                                                    aCTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatc                                                                           <  2:2887730/148‑1 (MQ=255)
                                                                     cTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatca                                                                          >  1:1921184/1‑148 (MQ=255)
                                                                      tGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTaataat                                                                         >  2:3424872/1‑148 (MQ=255)
                                                                       gTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatcatc                                                                        >  2:2448855/1‑148 (MQ=255)
                                                                        tttCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATcc                                                                       <  1:2509707/148‑1 (MQ=255)
                                                                            tgCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTGTTCGCTGTGTAATCATCCCAgg                                                                   >  1:1479655/2‑148 (MQ=255)
                                                                             gCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGt                                                                  <  2:3331769/148‑1 (MQ=255)
                                                                             gCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGt                                                                  <  1:717183/148‑1 (MQ=255)
                                                                             gCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGt                                                                  <  1:2477575/148‑1 (MQ=255)
                                                                             gCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGt                                                                  >  1:1272297/1‑148 (MQ=255)
                                                                              cAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTc                                                                 <  2:3113662/148‑1 (MQ=255)
                                                                                 cTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGc                                                              >  1:1141291/1‑148 (MQ=255)
                                                                                 cTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGc                                                              >  1:2927944/1‑148 (MQ=255)
                                                                                  tGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCg                                                             <  1:2785416/148‑1 (MQ=255)
                                                                                   ggAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCgg                                                            >  1:2368431/1‑147 (MQ=255)
                                                                                       aGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACg                                                        >  2:759317/1‑148 (MQ=255)
                                                                                        gTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGc                                                       <  1:2391073/148‑1 (MQ=255)
                                                                                        gTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGc                                                       <  1:1714060/148‑1 (MQ=255)
                                                                                         tATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGcc                                                      >  1:527366/1‑148 (MQ=255)
                                                                                           ttCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAg                                                    >  1:1439272/1‑148 (MQ=255)
                                                                                                 ccgccgACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAg                                              <  1:54606/148‑1 (MQ=255)
                                                                                                    ccgACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGa                                           >  2:465002/1‑148 (MQ=255)
                                                                                                       aCAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATaa                                        <  2:2265002/148‑1 (MQ=255)
                                                                                                       aCAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATaa                                        <  1:2086705/148‑1 (MQ=255)
                                                                                                         aaTCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAac                                      <  2:2604715/148‑1 (MQ=255)
                                                                                                          aTCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAaca                                     >  2:435712/1‑148 (MQ=255)
                                                                                                           tCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAacac                                    >  2:1079489/1‑148 (MQ=255)
                                                                                                             aCGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCg                                  >  1:2293245/1‑148 (MQ=255)
                                                                                                             aCGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCg                                  <  1:2179970/148‑1 (MQ=255)
                                                                                                             aCGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCg                                  >  2:3319280/1‑148 (MQ=255)
                                                                                                             aCGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCg                                  <  1:3461884/148‑1 (MQ=255)
                                                                                                               gATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCt                                <  1:506580/148‑1 (MQ=255)
                                                                                                                aTCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTg                               <  2:1028721/148‑1 (MQ=255)
                                                                                                                 tctcAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGa                              >  2:2208564/1‑148 (MQ=255)
                                                                                                                  ctcAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAg                             >  2:2405719/1‑148 (MQ=255)
                                                                                                                     aGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAgcgg                          <  2:1082915/148‑1 (MQ=255)
                                                                                                                       cATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAgcggcg                        >  1:1718771/1‑148 (MQ=255)
                                                                                                                       cATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAgcggcg                        >  2:1134539/1‑148 (MQ=255)
                                                                                                                        aTTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGa                       >  2:973094/1‑148 (MQ=255)
                                                                                                                           ttCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGAccc                    <  2:1318300/148‑1 (MQ=255)
                                                                                                                           ttCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGAccc                    <  2:1317376/148‑1 (MQ=255)
                                                                                                                                tCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCaa               >  1:438216/1‑148 (MQ=255)
                                                                                                                                 cgcgGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAAc              >  1:2030731/1‑148 (MQ=255)
                                                                                                                                 cgcgGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGTCCCTGCAAc              >  2:1485027/1‑148 (MQ=255)
                                                                                                                                  gcgGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACt             <  1:2788383/148‑1 (MQ=255)
                                                                                                                                      cAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTc         <  1:418407/148‑1 (MQ=255)
                                                                                                                                          gggATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGCa     <  2:249009/148‑1 (MQ=255)
                                                                                                                                           ggATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGCaa    <  1:380623/148‑1 (MQ=255)
                                                                                                                                           ggATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGCaa    <  2:98935/148‑1 (MQ=255)
                                                                                                                                             aTCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGCAAtt  >  1:1656406/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGCAATT  >  CP009273/4219714‑4220002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑