Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 27,307 C→T 100% I5I (ATC→ATT)  rihC → ribonucleoside hydrolase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927327,3070CT99.3% 973.8 / ‑2.9 293I5I (ATC→ATT) rihCribonucleoside hydrolase 3
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (145/145);  total (147/146)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 296 total aligned reads displayed.

GCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCA  >  CP009273/27160‑27453
                                                                                                                                                   |                                                                                                                                                  
gCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTAtttt                                                                                                                                                  <  1:3332423/150‑1 (MQ=255)
gCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTAtttt                                                                                                                                                  <  1:4060309/150‑1 (MQ=255)
   gTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCt                                                                                                                                               >  2:4436150/1‑150 (MQ=255)
     gaagaaAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCg                                                                                                                                             >  2:2796668/1‑150 (MQ=255)
     gaagaaAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCg                                                                                                                                             >  1:3654853/1‑150 (MQ=255)
       agaaAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGAt                                                                                                                                           >  1:4593063/1‑150 (MQ=255)
       agaaAACAGTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATGAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGAt                                                                                                                                           <  2:1012200/150‑1 (MQ=255)
        gaaAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATa                                                                                                                                          >  1:3199229/1‑150 (MQ=255)
        gaaAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATa                                                                                                                                          <  1:3427917/150‑1 (MQ=255)
         aaaaCATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATAc                                                                                                                                         >  1:4178674/1‑150 (MQ=255)
          aaaCATTGGTTTCGAAGGGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACt                                                                                                                                        <  2:3454801/150‑1 (MQ=255)
           aaCATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTg                                                                                                                                       >  2:551797/1‑150 (MQ=255)
                 gTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCg                                                                                                                                 <  2:3604741/150‑1 (MQ=255)
                        aaGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGa                                                                                                                          <  1:4314669/150‑1 (MQ=255)
                         aGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGAc                                                                                                                         >  1:2683090/1‑150 (MQ=255)
                         aGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGAc                                                                                                                         >  1:1346391/1‑150 (MQ=255)
                          gTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACg                                                                                                                        >  2:4136500/1‑150 (MQ=255)
                           tGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGTCGa                                                                                                                       >  2:4381787/1‑150 (MQ=255)
                            gCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGAt                                                                                                                      <  1:4273253/150‑1 (MQ=255)
                            gCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGAt                                                                                                                      >  1:2914122/1‑150 (MQ=255)
                                  agagCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTc                                                                                                                >  1:3020945/1‑150 (MQ=255)
                                  agagCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTc                                                                                                                >  2:2707800/1‑150 (MQ=255)
                                  agagCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTc                                                                                                                <  2:3199219/150‑1 (MQ=255)
                                   gagCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCg                                                                                                               <  1:626672/150‑1 (MQ=255)
                                      cTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCa                                                                                                            <  1:1039200/150‑1 (MQ=255)
                                       tGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCAt                                                                                                           >  2:2572087/1‑150 (MQ=255)
                                         cGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTg                                                                                                         <  1:1948914/150‑1 (MQ=255)
                                          gtgtCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTgc                                                                                                        >  2:3820547/1‑150 (MQ=255)
                                           tgtCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTgcc                                                                                                       <  2:9766/150‑1 (MQ=255)
                                           tgtCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTgcc                                                                                                       <  1:3992312/150‑1 (MQ=255)
                                            gtCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTgccg                                                                                                      >  1:4996222/1‑150 (MQ=255)
                                              cGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTgccgcc                                                                                                    >  2:2364443/1‑150 (MQ=255)
                                              cGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTgccgcc                                                                                                    >  1:5110092/1‑150 (MQ=255)
                                                   ttCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGAt                                                                                               <  2:3698780/150‑1 (MQ=255)
                                                    tCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGAtt                                                                                              <  2:1206456/150‑1 (MQ=255)
                                                        gAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTtgtg                                                                                          >  2:349366/1‑150 (MQ=255)
                                                         aaGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAAAATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGc                                                                                         >  1:3737672/1‑150 (MQ=255)
                                                          aGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCa                                                                                        <  1:940280/150‑1 (MQ=255)
                                                          aGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCa                                                                                        <  1:1221809/150‑1 (MQ=255)
                                                           gTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCAc                                                                                       >  1:4366263/1‑150 (MQ=255)
                                                            tCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCAcc                                                                                      <  1:23861/150‑1 (MQ=255)
                                                             cGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCAccc                                                                                     <  1:310599/150‑1 (MQ=255)
                                                             cGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCAccc                                                                                     <  1:2643951/150‑1 (MQ=255)
                                                                 tAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAAc                                                                                 >  2:2471800/1‑150 (MQ=255)
                                                                 tAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAAc                                                                                 <  1:2763584/150‑1 (MQ=255)
                                                                 tAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAAc                                                                                 <  2:4597316/150‑1 (MQ=255)
                                                                  aaGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACt                                                                                <  2:4519427/150‑1 (MQ=255)
                                                                  aaGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACt                                                                                <  1:1640869/150‑1 (MQ=255)
                                                                   aGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTc                                                                               >  1:149359/1‑150 (MQ=255)
                                                                    gTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTca                                                                              <  2:209371/150‑2 (MQ=255)
                                                                     tCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGa                                                                             >  2:189512/1‑150 (MQ=255)
                                                                        ttAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCt                                                                          >  2:5163686/1‑150 (MQ=255)
                                                                         tAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTg                                                                         >  1:3831864/1‑150 (MQ=255)
                                                                          agcagcCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGc                                                                        <  1:2466855/150‑1 (MQ=255)
                                                                          agcagcCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGc                                                                        >  2:4034839/1‑150 (MQ=255)
                                                                             agcCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGGTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAAc                                                                     >  1:1995815/1‑150 (MQ=255)
                                                                               ccTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACtg                                                                   >  1:87428/1‑150 (MQ=255)
                                                                                 tAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACtgat                                                                 <  2:3587438/150‑1 (MQ=255)
                                                                                  aaGTGATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATGTTTGCACCCGAACTCGACCTGCAACtgatg                                                                <  2:1643890/150‑1 (MQ=255)
                                                                                   aGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACtgatga                                                               >  1:3275158/1‑150 (MQ=255)
                                                                                    gtgaTGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGac                                                              <  2:4804031/147‑1 (MQ=255)
                                                                                    gTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGac                                                              <  1:2613159/150‑1 (MQ=255)
                                                                                     ttATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGacc                                                             <  2:4211986/150‑1 (MQ=255)
                                                                                        tGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGaccacc                                                          >  2:5052289/1‑150 (MQ=255)
                                                                                          cGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACAGt                                                        >  1:3542254/1‑150 (MQ=255)
                                                                                           gAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTc                                                       >  2:442915/1‑150 (MQ=255)
                                                                                             aaaTGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTcgc                                                     <  2:1439612/150‑1 (MQ=255)
                                                                                              taTGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTcgcg                                                    >  2:3307096/2‑150 (MQ=255)
                                                                                              aaTGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTcgcg                                                    >  1:1688054/1‑150 (MQ=255)
                                                                                                   cGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTaa                                               <  2:499476/150‑1 (MQ=255)
                                                                                                    ggTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAAt                                              >  1:4880677/1‑150 (MQ=255)
                                                                                                      tCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGt                                            >  1:4450837/1‑150 (MQ=255)
                                                                                                       cTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGtc                                           <  2:1346391/150‑1 (MQ=255)
                                                                                                       cTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGtc                                           >  1:4834417/1‑150 (MQ=255)
                                                                                                         tGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGtctc                                         <  1:4941283/150‑1 (MQ=255)
                                                                                                          gTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCg                                        <  1:1122678/150‑1 (MQ=255)
                                                                                                          gTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCg                                        <  2:3132278/150‑1 (MQ=255)
                                                                                                           ttACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCgg                                       <  2:4072251/150‑1 (MQ=255)
                                                                                                              ccGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTg                                    >  2:2218333/1‑150 (MQ=255)
                                                                                                                ggCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTgag                                  <  2:4879817/150‑1 (MQ=255)
                                                                                                                ggCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAAGGTCTCGGTTgag                                  <  2:4669204/150‑1 (MQ=255)
                                                                                                                   aTTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGaaa                               >  2:3553867/1‑150 (MQ=255)
                                                                                                                      ttttATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACt                            <  1:752898/150‑1 (MQ=255)
                                                                                                                      ttttATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACt                            <  2:921765/150‑1 (MQ=255)
                                                                                                                        ttATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTAc                          >  2:3875915/1‑150 (MQ=255)
                                                                                                                        ttATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTAc                          >  2:3762365/1‑150 (MQ=255)
                                                                                                                        ttATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTAc                          <  1:3526650/150‑1 (MQ=255)
                                                                                                                           tGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCg                       >  1:5075941/1‑150 (MQ=255)
                                                                                                                             gagaAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCa                     >  1:2220474/1‑150 (MQ=255)
                                                                                                                              agaAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCaa                    >  1:4124993/1‑150 (MQ=255)
                                                                                                                               gaAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAAt                   <  2:3543774/150‑1 (MQ=255)
                                                                                                                                 aaaCATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACTATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATg                  >  2:3434490/1‑149 (MQ=255)
                                                                                                                                 aaaCATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGc                 <  2:2914122/150‑1 (MQ=255)
                                                                                                                                      tGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGc            >  1:3946003/1‑150 (MQ=255)
                                                                                                                                        cGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCaa          <  2:199058/150‑1 (MQ=255)
                                                                                                                                         gTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAAc         >  2:2328597/1‑150 (MQ=255)
                                                                                                                                          tttACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAAct        >  1:1763910/1‑150 (MQ=255)
                                                                                                                                            tACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCaa                    <  1:1583049/136‑1 (MQ=255)
                                                                                                                                            tACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCaa                    >  2:1583014/1‑136 (MQ=255)
                                                                                                                                            tACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAActgc      >  1:3256584/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCA  >  CP009273/27160‑27453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑