Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,826,247 A→T 83.9% Y551N (TAC→AAC)  yicI ← putative alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,826,2470AT83.9% 770.2 / 128.2 299Y551N (TAC→AAC) yicIputative alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base A (20/28);  new base T (127/124);  total (147/152)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01

Only 100 of 299 total aligned reads displayed.

CGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGG  >  CP009273/3826101‑3826395
                                                                                                                                                  |                                                                                                                                                    
cGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGc                                                                                                                                                   >  1:4966940/1‑150 (MQ=255)
cGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGc                                                                                                                                                   <  2:4531552/150‑1 (MQ=255)
 ggATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGcc                                                                                                                                                  >  2:3152499/1‑150 (MQ=255)
  gATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCAGCATCGTTGGccc                                                                                                                                                 >  2:3095350/1‑150 (MQ=255)
    tCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCAc                                                                                                                                               >  1:1563178/1‑150 (MQ=255)
    tCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCAc                                                                                                                                               <  1:294386/150‑1 (MQ=255)
     cGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACg                                                                                                                                              <  2:1563026/150‑1 (MQ=255)
     cGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACg                                                                                                                                              >  1:2605535/1‑150 (MQ=255)
       tCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGc                                                                                                                                            <  2:2371873/150‑1 (MQ=255)
       tCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGc                                                                                                                                            <  1:813875/150‑1 (MQ=255)
        ccGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGca                                                                                                                                           <  2:1188404/150‑1 (MQ=255)
          gggAACTCCATCATCATGGCCCGCATCCTCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGcaca                                                                                                                                         <  2:4179766/150‑1 (MQ=255)
          gggAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGcaca                                                                                                                                         <  1:3745035/150‑1 (MQ=255)
           ggAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCacac                                                                                                                                        >  1:2617537/1‑150 (MQ=255)
              aCTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGAt                                                                                                                                     <  2:858303/150‑1 (MQ=255)
               cTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATa                                                                                                                                    <  2:4902528/150‑1 (MQ=255)
                tCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATaa                                                                                                                                   >  2:1670185/1‑150 (MQ=255)
                 ccATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAg                                                                                                                                  >  2:1069461/1‑150 (MQ=255)
                 ccATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAg                                                                                                                                  <  2:556812/150‑1 (MQ=255)
                    tcatcatGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGAtt                                                                                                                               <  2:3813782/150‑1 (MQ=255)
                     catcatGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGAttt                                                                                                                              >  1:2630142/1‑150 (MQ=255)
                     catcatGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGAttt                                                                                                                              <  2:4658126/150‑1 (MQ=255)
                          tGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTAc                                                                                                                         <  1:3140549/150‑1 (MQ=255)
                              ccGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCgtg                                                                                                                     >  1:309772/1‑150 (MQ=255)
                              ccGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCgtg                                                                                                                     >  1:3971855/1‑150 (MQ=255)
                                gCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTa                                                                                                                   <  2:4144101/150‑1 (MQ=255)
                                 catcatCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTaa                                                                                                                  <  1:5045887/150‑1 (MQ=255)
                                  atcatcGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATACGATTTGCTACCGTGTaaa                                                                                                                 >  2:26062/1‑150 (MQ=255)
                                          cGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATg                                                                                                         <  2:228268/150‑1 (MQ=255)
                                            tACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGc                                                                                                       >  1:4481838/1‑150 (MQ=255)
                                               cccGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACCTCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTgg                                                                                                    <  2:1937442/150‑1 (MQ=255)
                                               cccGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTgg                                                                                                    >  1:2364678/1‑150 (MQ=255)
                                                 cgcgcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGgag                                                                                                  >  1:1191344/1‑150 (MQ=255)
                                                 cgcgcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGgag                                                                                                  >  2:1170479/1‑150 (MQ=255)
                                                 cgcgcgTTCGCACGCGCAGCTTCACGATACAGAGACGGCATCATGCGGCATTTCAGGTGCGTGAAGAAGCGCACCACCTCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGgag                                                                                                  <  2:3951436/150‑1 (MQ=255)
                                                    gcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGc                                                                                               >  2:4483439/1‑150 (MQ=255)
                                                      gTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGcaa                                                                                             <  2:1227315/150‑1 (MQ=255)
                                                        tCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGcaaac                                                                                           <  1:3529691/150‑1 (MQ=255)
                                                        tCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGcaaac                                                                                           <  2:4798690/150‑1 (MQ=255)
                                                         cGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGcaaacc                                                                                          >  1:340332/1‑150 (MQ=255)
                                                             cgcgCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGcaaaccaaac                                                                                      <  1:1138617/150‑1 (MQ=255)
                                                              gcgcAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAAcg                                                                                     <  1:4128113/150‑1 (MQ=255)
                                                              gcgcAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAAcg                                                                                     <  2:2859548/150‑1 (MQ=255)
                                                               cgcAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAAcgc                                                                                    >  1:4614238/1‑150 (MQ=255)
                                                               cgcAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAAcgc                                                                                    >  1:2533218/1‑150 (MQ=255)
                                                                  aGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCa                                                                                 >  2:2969114/1‑150 (MQ=255)
                                                                       cACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGc                                                                            <  2:2777403/150‑1 (MQ=255)
                                                                        aCGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCg                                                                           <  2:1503528/150‑1 (MQ=255)
                                                                        aCGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCg                                                                           <  1:3228638/150‑1 (MQ=255)
                                                                         cGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGt                                                                          >  1:1289058/1‑150 (MQ=255)
                                                                         cGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGt                                                                          >  2:3694995/1‑150 (MQ=255)
                                                                         cGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGt                                                                          >  2:3251964/1‑150 (MQ=255)
                                                                          gATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGtt                                                                         >  1:326322/1‑150 (MQ=255)
                                                                           aTACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGttt                                                                        >  2:2528994/1‑150 (MQ=255)
                                                                            tACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTg                                                                       <  1:4034984/150‑1 (MQ=255)
                                                                            tACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTg                                                                       <  1:1452749/150‑1 (MQ=255)
                                                                                gATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTaaa                                                                   >  1:5129857/1‑150 (MQ=255)
                                                                                gATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTaaa                                                                   >  1:3798849/1‑150 (MQ=255)
                                                                                 aTACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAAc                                                                  <  1:2744828/150‑1 (MQ=255)
                                                                                 aTACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAAc                                                                  <  2:3230590/150‑1 (MQ=255)
                                                                                 aTACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAAc                                                                  <  1:673766/150‑1 (MQ=255)
                                                                                 aTACGGCATCATGCGGCATTTCAGGTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAAc                                                                  <  1:584394/150‑1 (MQ=255)
                                                                                      gCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTgcg                                                             <  1:2071334/150‑1 (MQ=255)
                                                                                       catcatGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTgcgc                                                            <  2:257211/150‑1 (MQ=255)
                                                                                         tcatGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCg                                                          <  1:4512756/150‑1 (MQ=255)
                                                                                          catGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCgg                                                         <  2:3733787/150‑1 (MQ=255)
                                                                                             gCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGc                                                      <  1:1635408/150‑1 (MQ=255)
                                                                                             gCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGc                                                      <  2:4443878/150‑1 (MQ=255)
                                                                                              cGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCg                                                     <  1:2312375/150‑1 (MQ=255)
                                                                                              cGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCg                                                     >  1:4671489/1‑150 (MQ=255)
                                                                                               ggCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCgg                                                    >  1:3336948/1‑150 (MQ=255)
                                                                                                gCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGt                                                   >  1:3911157/1‑150 (MQ=255)
                                                                                                   tttCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTAtt                                                >  2:1152711/1‑150 (MQ=255)
                                                                                                   tttCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTAtt                                                <  1:1909033/150‑1 (MQ=255)
                                                                                                     tCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTAtttt                                              <  2:4926596/150‑1 (MQ=255)
                                                                                                     tCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTAtttt                                              <  1:3050253/150‑1 (MQ=255)
                                                                                                        gTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCaa                                           >  2:881064/1‑150 (MQ=255)
                                                                                                        gTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCaa                                           >  2:2311299/1‑150 (MQ=255)
                                                                                                        gTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCaa                                           >  2:1490236/1‑150 (MQ=255)
                                                                                                            cGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAgcc                                       <  1:4334534/150‑1 (MQ=255)
                                                                                                                aagaagCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGccgccg                                   <  1:2684955/150‑1 (MQ=255)
                                                                                                                   aagCGCACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGata                                >  2:251009/1‑150 (MQ=255)
                                                                                                                    agCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGatat                               >  1:5111352/1‑150 (MQ=255)
                                                                                                                    agCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGatat                               >  2:4111652/1‑150 (MQ=255)
                                                                                                                      cgcACCACATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCg                             >  2:3520829/1‑150 (MQ=255)
                                                                                                                        caccacATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTg                           <  1:2440313/150‑1 (MQ=255)
                                                                                                                         accacATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTgg                          <  2:339820/150‑1 (MQ=255)
                                                                                                                          ccacATCACAGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGc                         >  1:4419103/1‑150 (MQ=255)
                                                                                                                              aTCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCa                     >  2:1544837/1‑150 (MQ=255)
                                                                                                                                 acaGGACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGaa                  >  2:4761186/1‑150 (MQ=255)
                                                                                                                                     gACTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCGACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCa              <  2:5016763/150‑1 (MQ=255)
                                                                                                                                      aCTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCaa             >  1:3599184/1‑150 (MQ=255)
                                                                                                                                      aCTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCaa             <  1:4119979/150‑1 (MQ=255)
                                                                                                                                       cTCATCATCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCaaa            >  2:735960/1‑150 (MQ=255)
                                                                                                                                         catcatCGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAAc          <  1:1397654/150‑1 (MQ=255)
                                                                                                                                          atcatcGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAAcc         <  2:5141808/150‑1 (MQ=255)
                                                                                                                                           tcatcGTTGTCCCACTGCTCACGATAAGATTTGCTACCGTGTAAACTGCTATGGCTGGTGAGCAAACCAAACGCGCACCAGCGATTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCt        <  2:2628500/150‑1 (MQ=255)
                                                                                                                                            catcGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTg       <  2:4234623/150‑1 (MQ=255)
                                                                                                                                            catcGTTGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTg       >  1:5086986/1‑150 (MQ=255)
                                                                                                                                                 tAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAgg  >  2:2225795/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGG  >  CP009273/3826101‑3826395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑