Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,828,206 T→A 100% S362C (AGT→TGT)  yicJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,828,2060TA98.5% 880.1 / 1.5 263S362C (AGT→TGT) yicJputative transporter
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base A (123/136);  total (125/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 263 total aligned reads displayed.

CGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAA  >  CP009273/3828060‑3828352
                                                                                                                                                  |                                                                                                                                                  
  tGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATcc                                                                                                                                               >  2:4154337/1‑150 (MQ=255)
  tGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATcc                                                                                                                                               >  1:2440781/1‑150 (MQ=255)
   gAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATccc                                                                                                                                              >  1:3721232/1‑150 (MQ=255)
    aaTAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCg                                                                                                                                             <  1:1110696/150‑1 (MQ=255)
    aaTAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCg                                                                                                                                             <  2:3288277/150‑1 (MQ=255)
     aTAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCgg                                                                                                                                            <  2:4400782/150‑1 (MQ=255)
      tAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGt                                                                                                                                           <  1:647767/150‑1 (MQ=255)
       aGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTc                                                                                                                                          >  1:3469569/1‑150 (MQ=255)
         cgcAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAg                                                                                                                                        >  2:1394229/1‑150 (MQ=255)
               aatGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGttt                                                                                                                                  <  2:2771818/150‑1 (MQ=255)
                atgatgCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTa                                                                                                                                 <  1:20327/150‑1 (MQ=255)
                 tgatgCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACTGATCCCGGTCAGGCGTTTAc                                                                                                                                >  1:628203/1‑150 (MQ=255)
                     gCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCAtt                                                                                                                            >  2:2672556/1‑150 (MQ=255)
                      cTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTg                                                                                                                           <  1:3335366/150‑1 (MQ=255)
                        aaTCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACACACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGca                                                                                                                         >  2:2816946/1‑150 (MQ=255)
                        aaTCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGca                                                                                                                         >  1:420839/1‑150 (MQ=255)
                        aaTCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGca                                                                                                                         >  2:4951709/1‑150 (MQ=255)
                         aTCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcac                                                                                                                        >  2:378584/1‑150 (MQ=255)
                          tCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcacc                                                                                                                       >  2:433740/1‑150 (MQ=255)
                           cGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcacca                                                                                                                      <  2:2355799/150‑1 (MQ=255)
                            gTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcaccac                                                                                                                     <  1:2893169/150‑1 (MQ=255)
                            gTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcaccac                                                                                                                     <  1:317193/150‑1 (MQ=255)
                              ggCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTc                                                                                                                   >  2:3183957/1‑150 (MQ=255)
                               gcgcTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCg                                                                                                                  <  2:3942961/150‑1 (MQ=255)
                               gcgcTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCg                                                                                                                  <  2:1409987/150‑1 (MQ=255)
                                 gcTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGcc                                                                                                                >  2:3094786/1‑150 (MQ=255)
                                    gTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTa                                                                                                             >  2:3281779/1‑150 (MQ=255)
                                     ttCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCTCCACTCGCCGTAg                                                                                                            <  1:4739759/150‑1 (MQ=255)
                                       cTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTc                                                                                                          <  1:2456815/150‑1 (MQ=255)
                                       cTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTc                                                                                                          <  1:2544187/150‑1 (MQ=255)
                                        tGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCg                                                                                                         <  2:2453017/150‑1 (MQ=255)
                                         gcgcTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGa                                                                                                        >  1:3810506/1‑150 (MQ=255)
                                          cgcTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGAc                                                                                                       <  1:482703/150‑1 (MQ=255)
                                           gcTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACg                                                                                                      <  1:78092/150‑1 (MQ=255)
                                                  cTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCgg                                                                                               >  2:1096912/1‑150 (MQ=255)
                                                   tGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGa                                                                                              >  1:3137654/1‑150 (MQ=255)
                                                    gCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAc                                                                                             >  1:2225301/1‑150 (MQ=255)
                                                      cGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAcat                                                                                           >  2:824983/1‑150 (MQ=255)
                                                      cGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAcat                                                                                           >  2:1682055/1‑150 (MQ=255)
                                                       gCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAcatc                                                                                          <  1:3964337/150‑1 (MQ=255)
                                                       gCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAcatc                                                                                          <  2:1161180/150‑1 (MQ=255)
                                                        catcatATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAcatca                                                                                         <  1:3454525/150‑1 (MQ=255)
                                                        catcatATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAcatca                                                                                         >  1:3733359/1‑150 (MQ=255)
                                                            atatCCGCCATCAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTAc                                                                                     <  2:1590094/150‑1 (MQ=255)
                                                             tatCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTAcc                                                                                    >  2:2934952/1‑150 (MQ=255)
                                                              atCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTAccc                                                                                   >  1:2414823/1‑150 (MQ=255)
                                                                 cGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACt                                                                                >  2:4348723/1‑150 (MQ=255)
                                                                  gCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTCTCGGACATCATTACCCACTg                                                                               >  2:1873452/1‑150 (MQ=255)
                                                                     aTAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGAt                                                                            <  1:2604833/150‑1 (MQ=255)
                                                                     aTAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGAt                                                                            >  2:2454491/1‑150 (MQ=255)
                                                                     aTAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGAt                                                                            <  1:1375041/150‑1 (MQ=255)
                                                                     aTAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACGCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGAt                                                                            >  2:3450307/1‑150 (MQ=255)
                                                                      tAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATa                                                                           <  1:287386/150‑1 (MQ=255)
                                                                        aGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAgg                                                                         >  2:2264653/1‑150 (MQ=255)
                                                                         gCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGgt                                                                        >  2:3626904/1‑150 (MQ=255)
                                                                         gCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGgt                                                                        <  2:1317362/150‑1 (MQ=255)
                                                                           cAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGgtgt                                                                      >  2:851789/1‑150 (MQ=255)
                                                                            aGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTc                                                                     <  2:46898/150‑1 (MQ=255)
                                                                            aGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTc                                                                     >  1:5132807/1‑150 (MQ=255)
                                                                            aGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTc                                                                     <  1:412947/150‑1 (MQ=255)
                                                                               aTCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCAcc                                                                  <  1:1030485/150‑1 (MQ=255)
                                                                                       gATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATg                                                          <  2:1143706/150‑1 (MQ=255)
                                                                                       gATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATg                                                          >  1:4496601/1‑150 (MQ=255)
                                                                                            gagCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAaca                                                     <  2:1460214/150‑1 (MQ=255)
                                                                                              gcgcCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAacaca                                                   <  1:4810661/150‑1 (MQ=255)
                                                                                               cgccgcCGAAGGCCAACCCCCGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAacacac                                                  <  1:40258/150‑1 (MQ=255)
                                                                                                   gccgAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAAt                                              <  2:606837/150‑1 (MQ=255)
                                                                                                    ccgAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATc                                             >  2:3505664/1‑150 (MQ=255)
                                                                                                    ccgAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATc                                             >  1:4002572/1‑150 (MQ=255)
                                                                                                     cgAATGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCa                                            <  1:3404861/150‑1 (MQ=255)
                                                                                                     cgAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCa                                            <  1:819023/150‑1 (MQ=255)
                                                                                                      gAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCAc                                           >  1:3063287/1‑150 (MQ=255)
                                                                                                       aaGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACg                                          <  2:2117221/150‑1 (MQ=255)
                                                                                                       aaGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACg                                          <  2:4797430/150‑1 (MQ=255)
                                                                                                        aGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGa                                         <  2:1226801/150‑1 (MQ=255)
                                                                                                           ccAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGa                                      >  2:3855006/1‑150 (MQ=255)
                                                                                                           ccAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGa                                      <  1:294703/150‑1 (MQ=255)
                                                                                                              aCCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGa                                   <  1:626679/150‑1 (MQ=255)
                                                                                                                  cAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGAc                               >  1:3100414/1‑150 (MQ=255)
                                                                                                                      ttGAGCACAAACAGCGTGCCAGCAAAACTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAAc                           >  1:3773863/1‑150 (MQ=255)
                                                                                                                      ttGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCAATGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGAAGAAGACAAAc                           >  1:1697578/1‑150 (MQ=255)
                                                                                                                          gCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAg                       <  1:1597929/150‑1 (MQ=255)
                                                                                                                           cacaAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGt                      <  1:1539272/150‑1 (MQ=255)
                                                                                                                            acaAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTg                     >  1:2296958/1‑150 (MQ=255)
                                                                                                                            acaAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTg                     <  2:1375725/150‑1 (MQ=255)
                                                                                                                                tcAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGc                 >  1:5000991/2‑150 (MQ=255)
                                                                                                                                aCAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGc                 <  1:398154/150‑1 (MQ=255)
                                                                                                                                 cAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCt                >  1:3613506/1‑150 (MQ=255)
                                                                                                                                 cAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCt                <  1:4348723/150‑1 (MQ=255)
                                                                                                                                 cAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCt                <  2:702228/150‑1 (MQ=255)
                                                                                                                                 cAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCt                <  2:3733359/150‑1 (MQ=255)
                                                                                                                                  aGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGCCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTg               <  2:4893084/150‑1 (MQ=255)
                                                                                                                                     gTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGcc            >  1:1216975/1‑150 (MQ=255)
                                                                                                                                     gTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGcc            <  2:525311/150‑1 (MQ=255)
                                                                                                                                      tGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCt           >  1:2723703/1‑150 (MQ=255)
                                                                                                                                         cAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCa        <  2:2225114/150‑1 (MQ=255)
                                                                                                                                          aGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCAt       >  2:3587965/1‑150 (MQ=255)
                                                                                                                                             aaaaCAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATggg    >  2:5085766/1‑150 (MQ=255)
                                                                                                                                             aaaaCAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATggg    <  2:962953/150‑1 (MQ=255)
                                                                                                                                              aaaCAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGa   <  2:2349634/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAA  >  CP009273/3828060‑3828352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑