Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 319,024 C→A 100% R326S (CGC→AGC)  ykgF → ferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273319,0240CA98.8% 803.4 / ‑0.2 245R326S (CGC→AGC) ykgFferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base A (111/131);  total (111/134)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 245 total aligned reads displayed.

CCGCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGT  >  CP009273/318877‑319169
                                                                                                                                                   |                                                                                                                                                 
     cgAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCatat                                                                                                                                            >  1:3493574/1‑150 (MQ=255)
       aaGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGTCATATAGCCATATTg                                                                                                                                          >  2:2109031/1‑150 (MQ=255)
        aGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTgg                                                                                                                                         <  2:1923029/150‑1 (MQ=255)
         gCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGc                                                                                                                                        >  2:2500607/1‑150 (MQ=255)
                aCGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATg                                                                                                                                 >  1:4071518/1‑150 (MQ=255)
                aCGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATg                                                                                                                                 >  1:3874920/1‑150 (MQ=255)
                 cGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATgg                                                                                                                                <  1:57353/150‑1 (MQ=255)
                   ttGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGat                                                                                                                              <  2:4129259/150‑1 (MQ=255)
                   ttGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGat                                                                                                                              <  2:3167713/150‑1 (MQ=255)
                     gATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGatat                                                                                                                            >  1:1273356/1‑150 (MQ=255)
                        ggTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGc                                                                                                                         >  2:3518645/1‑150 (MQ=255)
                         gTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGct                                                                                                                        <  1:3324919/150‑1 (MQ=255)
                         gTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGct                                                                                                                        <  1:3324886/150‑1 (MQ=255)
                          tCCTGAAGAGTTTCATCGGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGctc                                                                                                                       <  2:2103023/150‑1 (MQ=255)
                            cTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTa                                                                                                                     >  2:1662394/1‑150 (MQ=255)
                            cTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTa                                                                                                                     <  2:3419004/150‑1 (MQ=255)
                             tGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTAt                                                                                                                    <  2:3268478/150‑1 (MQ=255)
                              gAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTAtt                                                                                                                   >  1:4132011/1‑150 (MQ=255)
                               aaGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTAttt                                                                                                                  <  1:4834691/150‑1 (MQ=255)
                               aaGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTAttt                                                                                                                  <  2:3840279/150‑1 (MQ=255)
                                 gagTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTAt                                                                                                                <  1:2920484/150‑1 (MQ=255)
                                  agTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATc                                                                                                               >  1:2839840/1‑150 (MQ=255)
                                   gTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATcc                                                                                                              >  1:4322198/1‑150 (MQ=255)
                                   gTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATcc                                                                                                              >  1:4493222/1‑150 (MQ=255)
                                        aTCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGc                                                                                                         >  1:1096320/1‑150 (MQ=255)
                                        aTCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGc                                                                                                         >  2:3903923/1‑150 (MQ=255)
                                            ggTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAAt                                                                                                     <  2:3663468/150‑1 (MQ=255)
                                             gTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAAtt                                                                                                    <  2:3352257/150‑1 (MQ=255)
                                              ttATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTg                                                                                                   <  1:4641760/150‑1 (MQ=255)
                                                 ttGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTg                                                                                                <  1:1860620/150‑1 (MQ=255)
                                                  tGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGc                                                                                               >  2:2609637/1‑150 (MQ=255)
                                                  tGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGc                                                                                               <  1:4770665/150‑1 (MQ=255)
                                                   gTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCg                                                                                              <  1:3594301/150‑1 (MQ=255)
                                                   gTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCg                                                                                              <  1:1612926/150‑1 (MQ=255)
                                                   gTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCg                                                                                              <  2:2022962/150‑1 (MQ=255)
                                                    tCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCgg                                                                                             >  2:4798359/1‑150 (MQ=255)
                                                     cGATTACGGGCGTTCTGAGTTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGt                                                                                            <  2:3141118/150‑1 (MQ=255)
                                                     cGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGt                                                                                            >  1:1085142/1‑150 (MQ=255)
                                                       aTAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATCTGGCTCTATTTATCCAGGGCCAAGTGGTGCGGTGa                                                                                          >  2:2999077/1‑150 (MQ=255)
                                                        tAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGAt                                                                                         <  2:3740738/150‑1 (MQ=255)
                                                            gggCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTtct                                                                                     >  2:4272690/1‑150 (MQ=255)
                                                            gggCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTtct                                                                                     >  2:1411873/1‑150 (MQ=255)
                                                            gggCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTtct                                                                                     <  2:2863229/150‑1 (MQ=255)
                                                             ggCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTtctc                                                                                    <  2:4452423/150‑1 (MQ=255)
                                                              gCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTcc                                                                                   <  1:4184768/150‑1 (MQ=255)
                                                               cGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCg                                                                                  <  2:1744474/150‑1 (MQ=255)
                                                               cGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCg                                                                                  >  1:819880/1‑150 (MQ=255)
                                                                gTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGc                                                                                 >  1:2600598/1‑150 (MQ=255)
                                                                gTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGc                                                                                 >  2:3516309/1‑150 (MQ=255)
                                                                 ttCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCt                                                                                <  2:4614466/150‑1 (MQ=255)
                                                                  tCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTa                                                                               >  2:1337647/1‑150 (MQ=255)
                                                                      aGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTg                                                                           <  1:1020177/150‑1 (MQ=255)
                                                                      aGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTg                                                                           <  2:4497915/150‑1 (MQ=255)
                                                                      aGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTg                                                                           <  1:2525784/150‑1 (MQ=255)
                                                                       ggTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                          >  2:4623508/1‑150 (MQ=255)
                                                                        gTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                         <  1:3040152/150‑1 (MQ=255)
                                                                         tGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTggcg                                                                        >  1:4256527/1‑150 (MQ=255)
                                                                          gCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTggcgg                                                                       <  1:4588517/150‑1 (MQ=255)
                                                                           cTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTggcggc                                                                      >  1:747867/1‑150 (MQ=255)
                                                                            tGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCt                                                                     >  2:3452432/1‑150 (MQ=255)
                                                                              gCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCtat                                                                   <  1:2365601/150‑1 (MQ=255)
                                                                              gCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCtat                                                                   <  2:4929520/150‑1 (MQ=255)
                                                                               ccTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCtata                                                                  <  2:4816357/150‑1 (MQ=255)
                                                                                   tGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaaga                                                              <  2:2375219/150‑1 (MQ=255)
                                                                                    gAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagat                                                             >  2:4582195/1‑150 (MQ=255)
                                                                                    gAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagat                                                             <  2:2584895/150‑1 (MQ=255)
                                                                                    gAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATATATCCAGGGCAAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagat                                                             >  1:3972867/1‑150 (MQ=255)
                                                                                     aaTTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagatt                                                            >  1:220680/1‑150 (MQ=255)
                                                                                     aaTTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagatt                                                            <  1:3774133/150‑1 (MQ=255)
                                                                                          cGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaa                                                       <  2:3258358/150‑1 (MQ=255)
                                                                                           gggATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaag                                                      <  1:5067056/150‑1 (MQ=255)
                                                                                                gtgCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTa                                                 >  2:767523/1‑150 (MQ=255)
                                                                                                    tgcGCTGTATTCGCTGAGGGGCTTGTATGAATACTTGTCCGGCATATATCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCTGCTATAAAGATTTTAAAGATTTACCCt                                             <  1:3914335/150‑1 (MQ=255)
                                                                                                     gcgcTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTa                                            <  2:539849/150‑1 (MQ=255)
                                                                                                      cgcTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTAc                                           >  2:378724/1‑150 (MQ=255)
                                                                                                      cgcTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTAc                                           <  1:4404883/150‑1 (MQ=255)
                                                                                                       gcTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACg                                          >  1:1479989/1‑150 (MQ=255)
                                                                                                       gcTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACg                                          <  2:424/150‑1 (MQ=255)
                                                                                                       gcTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCCATAAAGATTTTAAAGATTTACCCTACg                                          <  2:4322198/150‑1 (MQ=255)
                                                                                                        cTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGc                                         >  2:3597354/1‑150 (MQ=255)
                                                                                                         tGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGcc                                        >  1:3431025/1‑150 (MQ=255)
                                                                                                           tATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTg                                      <  2:704008/150‑1 (MQ=255)
                                                                                                             ttCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGct                                    >  2:1034363/1‑150 (MQ=255)
                                                                                                              tCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGctc                                   >  1:60018/1‑150 (MQ=255)
                                                                                                              tCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGctc                                   >  1:1882736/1‑150 (MQ=255)
                                                                                                               cGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGctct                                  >  2:1225451/1‑150 (MQ=255)
                                                                                                                tgagCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCtt                                 <  2:1795804/147‑1 (MQ=255)
                                                                                                                gCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCtt                                 >  1:728131/1‑150 (MQ=255)
                                                                                                                     cgggCTTGTATGAAAACTTGTCCGGCATAAAGCCATATTCGCGGTCAAGCATAAGGCTCTTTTTTTCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGc                            <  2:3985394/149‑1 (MQ=255)
                                                                                                                        gCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGcaca                         <  1:2165061/150‑1 (MQ=255)
                                                                                                                              aTGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTtgt                   >  1:1409130/1‑150 (MQ=255)
                                                                                                                                gAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGa                 >  1:1595590/1‑150 (MQ=255)
                                                                                                                                       tGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtg          >  1:1187989/1‑150 (MQ=255)
                                                                                                                                        gTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgt         >  1:2751105/1‑150 (MQ=255)
                                                                                                                                        gTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgt         <  1:2638459/150‑1 (MQ=255)
                                                                                                                                         tCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgtg        <  1:2756439/150‑1 (MQ=255)
                                                                                                                                         tCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgtg        >  1:174083/1‑150 (MQ=255)
                                                                                                                                          ccGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgtgt       >  1:4983145/1‑150 (MQ=255)
                                                                                                                                               atatAGCCATATTGGCGGTCATGGATTTGGCTCTATTTTTCCAGGGCCTATTGGTGCGGTGTTTTCTCCGCTACTTGGCGGCTTTAAAGATTTTTTTGATTTTCCCTAGGCGTGGTCTTTTTGCTCAGGTTGTGACTTGGTGTGTGCGGt  >  2:2148897/1‑150 (MQ=255)
                                                                                                                                               atatAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGt  <  1:97199/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCGCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGT  >  CP009273/318877‑319169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑