Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,830,819 T→G 100% F169L (TTT→TTG)  setC → putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,830,8190TG98.2% 973.3 / ‑0.5 254F169L (TTT→TTG) setCputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base T (3/4);  new base G (93/154);  total (96/158)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 254 total aligned reads displayed.

CGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCGGGA  >  CP009273/3830672‑3830965
                                                                                                                                                   |                                                                                                                                                  
  cAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGtt                                                                                                                                                <  2:2818278/150‑1 (MQ=255)
   aaaCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGttt                                                                                                                                               >  2:966880/1‑150 (MQ=255)
   aaaCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGttt                                                                                                                                               <  1:4240947/150‑1 (MQ=255)
      cccGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTaa                                                                                                                                            >  1:1594498/1‑150 (MQ=255)
           aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGa                                                                                                                                       >  1:2817636/1‑150 (MQ=255)
            aaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGAt                                                                                                                                      <  1:3939176/150‑1 (MQ=255)
               gTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTa                                                                                                                                   >  1:1687140/1‑150 (MQ=255)
                ttCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTAt                                                                                                                                  <  2:1379857/150‑1 (MQ=255)
                 tcgccctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTAtc                                                                                                                                 <  2:2987654/150‑1 (MQ=255)
                  cgccctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTAtct                                                                                                                                >  1:1820388/1‑150 (MQ=255)
                   gccctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTAtctc                                                                                                                               <  1:4137538/150‑1 (MQ=255)
                    ccctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCa                                                                                                                              >  1:3174697/1‑150 (MQ=255)
                       tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCg                                                                                                                           <  2:3128/150‑1 (MQ=255)
                         gcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCt                                                                                                                         <  2:5111506/150‑1 (MQ=255)
                         gcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCt                                                                                                                         <  2:1717893/150‑1 (MQ=255)
                         gcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCAGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCt                                                                                                                         <  2:2369501/150‑1 (MQ=255)
                           ccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGc                                                                                                                       >  1:4840513/1‑150 (MQ=255)
                           ccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGc                                                                                                                       <  2:2039821/150‑1 (MQ=255)
                            cGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCACTGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGcc                                                                                                                      >  1:2030971/1‑150 (MQ=255)
                             gTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCa                                                                                                                     <  2:5011950/150‑1 (MQ=255)
                             gTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCa                                                                                                                     <  1:4466379/150‑1 (MQ=255)
                               gAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATc                                                                                                                   <  1:4294192/150‑1 (MQ=255)
                                 acacGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCGTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGc                                                                                                                 <  2:4648066/150‑1 (MQ=255)
                                  cacGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCa                                                                                                                <  2:335428/150‑1 (MQ=255)
                                     gCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCAttt                                                                                                             <  2:308326/150‑1 (MQ=255)
                                     gCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCAttt                                                                                                             >  1:50501/1‑150 (MQ=255)
                                       cGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCaggtgg                                                                                                           >  2:3824947/1‑145 (MQ=255)
                                       cGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATttgt                                                                                                           >  2:3885729/1‑150 (MQ=255)
                                       cGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATttgt                                                                                                           >  1:1290425/1‑150 (MQ=255)
                                        gACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATttgtt                                                                                                          <  1:1414873/150‑1 (MQ=255)
                                         aCAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATttgttg                                                                                                         <  1:296445/150‑1 (MQ=255)
                                          cAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTtgttgt                                                                                                        <  1:572765/150‑1 (MQ=255)
                                           aGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTgttgtt                                                                                                       <  1:4921778/150‑1 (MQ=255)
                                           aGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTgttgtt                                                                                                       >  1:2437021/1‑150 (MQ=255)
                                            gAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGttt                                                                                                      >  1:1177074/1‑150 (MQ=255)
                                            gAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGttt                                                                                                      >  2:2481577/1‑150 (MQ=255)
                                             aaCAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTg                                                                                                     <  2:1528838/150‑1 (MQ=255)
                                               cAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCg                                                                                                   <  1:4412225/150‑1 (MQ=255)
                                                 ggCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGa                                                                                                 <  2:493364/150‑1 (MQ=255)
                                                    cGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTg                                                                                              <  2:789138/150‑1 (MQ=255)
                                                     gTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGa                                                                                             >  2:2725218/1‑150 (MQ=255)
                                                      tGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGAt                                                                                            <  1:355949/150‑1 (MQ=255)
                                                       gagaCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATa                                                                                           <  1:1013063/150‑1 (MQ=255)
                                                       gagaCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATa                                                                                           <  2:4422601/150‑1 (MQ=255)
                                                        agaCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAg                                                                                          <  2:2976093/150‑1 (MQ=255)
                                                        agaCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAg                                                                                          >  2:3232678/1‑150 (MQ=255)
                                                         gaCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGt                                                                                         <  2:1088671/150‑1 (MQ=255)
                                                         gaCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGt                                                                                         <  2:1219647/150‑1 (MQ=255)
                                                            ggTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTg                                                                                      <  1:896023/150‑1 (MQ=255)
                                                            ggTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTg                                                                                      <  1:2349199/150‑1 (MQ=255)
                                                            ggTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTg                                                                                      <  1:1931225/150‑1 (MQ=255)
                                                            ggTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTg                                                                                      <  2:1687140/150‑1 (MQ=255)
                                                               cATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgtt                                                                                   <  2:4280196/150‑1 (MQ=255)
                                                                ttGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttg                                                                                  <  2:4096111/149‑1 (MQ=255)
                                                                 tGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttgt                                                                                 >  1:107123/1‑150 (MQ=255)
                                                                           aTTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATc                                                                       <  2:2767742/150‑1 (MQ=255)
                                                                            tttttACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCa                                                                      >  2:588001/1‑150 (MQ=255)
                                                                             ttttACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCaa                                                                     <  2:1333448/150‑1 (MQ=255)
                                                                                tACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATAc                                                                  <  2:4501753/150‑1 (MQ=255)
                                                                                  cGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACaa                                                                <  2:4560935/150‑1 (MQ=255)
                                                                                    tGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAg                                                              >  1:3783344/1‑150 (MQ=255)
                                                                                     gCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGa                                                             <  2:2894825/150‑1 (MQ=255)
                                                                                     gCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGa                                                             <  2:3991928/150‑1 (MQ=255)
                                                                                          gaactcGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAatat                                                        >  2:393207/4‑150 (MQ=255)
                                                                                            tctcGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTc                                                      >  2:3516402/1‑150 (MQ=255)
                                                                                            tctcGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTc                                                      <  1:5086107/150‑1 (MQ=255)
                                                                                               cGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTg                                                   <  1:2112639/150‑1 (MQ=255)
                                                                                                    gCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGtt                                              <  1:1477282/150‑1 (MQ=255)
                                                                                                     ccTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTa                                             >  1:1356749/1‑150 (MQ=255)
                                                                                                       tGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTACTGTCGTTAcc                                           >  1:4092844/1‑150 (MQ=255)
                                                                                                         ggTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCa                                         >  1:2143568/1‑150 (MQ=255)
                                                                                                          gTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCaa                                        <  1:3325519/150‑1 (MQ=255)
                                                                                                          gTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCaa                                        <  2:2089154/150‑1 (MQ=255)
                                                                                                              tCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCg                                    <  2:5030542/150‑1 (MQ=255)
                                                                                                              acGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCg                                    <  2:4807746/149‑1 (MQ=255)
                                                                                                                gggCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTa                                  >  2:4608457/1‑150 (MQ=255)
                                                                                                                   ccACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGaa                               <  1:4157197/150‑1 (MQ=255)
                                                                                                                    cACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGaaa                              <  1:528900/150‑1 (MQ=255)
                                                                                                                    cACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGaaa                              <  1:3116295/150‑1 (MQ=255)
                                                                                                                       cgctcgctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAAttt                           <  2:3603514/150‑1 (MQ=255)
                                                                                                                       cgctcgctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAAttt                           >  2:523864/1‑150 (MQ=255)
                                                                                                                        gctcgctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAAtttt                          <  2:686825/150‑1 (MQ=255)
                                                                                                                              ttagtcaCTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTc                    <  2:2638867/144‑1 (MQ=255)
                                                                                                                              ttATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTc                    >  1:1557271/1‑150 (MQ=255)
                                                                                                                                aTGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTcca                  >  1:4693382/1‑150 (MQ=255)
                                                                                                                                aTGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTcca                  >  1:232433/1‑150 (MQ=255)
                                                                                                                                  gAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTccacc                <  1:3824947/150‑1 (MQ=255)
                                                                                                                                  gAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTccacc                <  1:4288317/150‑1 (MQ=255)
                                                                                                                                   aaCTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCAGTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTccaccc               >  2:456928/1‑150 (MQ=255)
                                                                                                                                   aaCTGGCAATGGGATAGAGTTTTAAAGAGATGTATCTCACCGCTGCCATAGAATTTGGTGTTAGCGGACTGATAGTCTGGGTGTTTTTGCCATCAATACAAAGAAAAATTCCTGTCGTTACCCAACACCTAGAAATTTTACCCTcaacac               >  2:1642801/1‑148 (MQ=255)
                                                                                                                                      tGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCcaca            >  1:2636395/1‑150 (MQ=255)
                                                                                                                                      tGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCcaca            <  1:3733536/150‑1 (MQ=255)
                                                                                                                                      tGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCcaca            >  2:3747596/1‑150 (MQ=255)
                                                                                                                                      tGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCcaca            <  2:189358/150‑1 (MQ=255)
                                                                                                                                           aTGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAg       <  2:1820388/150‑1 (MQ=255)
                                                                                                                                           aTGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAg       <  2:4233204/150‑1 (MQ=255)
                                                                                                                                            tGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGc      <  1:4543488/150‑1 (MQ=255)
                                                                                                                                             gggATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCg     <  2:4183275/150‑1 (MQ=255)
                                                                                                                                             gggATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCg     <  1:3680972/150‑1 (MQ=255)
                                                                                                                                                aTTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCGGGa  >  1:5073742/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCGGGA  >  CP009273/3830672‑3830965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑