Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,837,532 C→A 100% P70Q (CCG→CAG)  ade → cryptic adenine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,837,5320CA95.5% 807.7 / 25.1 264P70Q (CCG→CAG) adecryptic adenine deaminase
Reads supporting (aligned to +/- strand):  ref base C (6/6);  new base A (134/118);  total (140/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 264 total aligned reads displayed.

TTAGCCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACC  >  CP009273/3837384‑3837678
                                                                                                                                                    |                                                                                                                                                  
     cGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCttt                                                                                                                                              >  2:3403055/1‑150 (MQ=255)
     cGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCttt                                                                                                                                              >  1:3934560/1‑150 (MQ=255)
         tCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAg                                                                                                                                          >  1:4860053/1‑150 (MQ=255)
          cccGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGc                                                                                                                                         <  1:4995441/150‑1 (MQ=255)
            cGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCgg                                                                                                                                       >  1:3850184/1‑150 (MQ=255)
             gTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGa                                                                                                                                      >  2:3968110/1‑150 (MQ=255)
             gTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGa                                                                                                                                      <  2:567741/150‑1 (MQ=255)
              tGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGAt                                                                                                                                     >  1:1782018/1‑150 (MQ=255)
                  gACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGAt                                                                                                                                 <  1:4531268/150‑1 (MQ=255)
                   aCGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATg                                                                                                                                >  1:1276728/1‑150 (MQ=255)
                     gCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCt                                                                                                                              >  2:3752359/1‑150 (MQ=255)
                       tGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTcg                                                                                                                            <  2:1432765/150‑1 (MQ=255)
                           gCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCgcggc                                                                                                                        <  1:1918483/150‑1 (MQ=255)
                            ccGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCgcggcg                                                                                                                       >  1:940116/1‑150 (MQ=255)
                            ccGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCgcggcg                                                                                                                       >  1:223423/1‑150 (MQ=255)
                             cGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGgcgc                                                                                                                      <  1:2830373/150‑1 (MQ=255)
                                   tattatTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAAcggc                                                                                                                <  2:1561498/150‑1 (MQ=255)
                                        ttGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGc                                                                                                           <  1:2688390/150‑1 (MQ=255)
                                         tGATAATGTCTCGATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACCTTGCCGGTGTTGGCGCAGAATACCCTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGcc                                                                                                          <  2:295144/150‑1 (MQ=255)
                                          gATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCa                                                                                                         <  1:4498899/150‑1 (MQ=255)
                                           aTAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAg                                                                                                        <  1:2005290/150‑1 (MQ=255)
                                            tAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAgg                                                                                                       <  2:4378833/150‑1 (MQ=255)
                                            tAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAgg                                                                                                       >  1:1629692/1‑150 (MQ=255)
                                            tAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAgg                                                                                                       <  2:2749839/150‑1 (MQ=255)
                                               tGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGtt                                                                                                    >  2:1631134/1‑150 (MQ=255)
                                                 tctcTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTa                                                                                                  >  1:2849096/1‑150 (MQ=255)
                                                 tctcTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAAGTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGTTTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTa                                                                                                  <  2:3601135/150‑1 (MQ=255)
                                                    ctATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTg                                                                                               >  1:1698965/1‑150 (MQ=255)
                                                     tATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGa                                                                                              <  1:4373573/150‑1 (MQ=255)
                                                      aTTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGAt                                                                                             <  1:5127145/150‑1 (MQ=255)
                                                      aTTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGAGTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGAt                                                                                             >  2:4246429/1‑150 (MQ=255)
                                                           cGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCa                                                                                        >  2:3646518/1‑150 (MQ=255)
                                                            taCCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGAAGCTCAGGCTTAGCAGCGGATTGATCCTCGCGGCGAACCGGCGGTGCCAGGGTTTATTTATGCTCAc                                                                                       >  2:2660162/2‑150 (MQ=255)
                                                                tGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGc                                                                                   >  1:1103412/1‑150 (MQ=255)
                                                                  aTCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCat                                                                                 >  2:2184491/1‑150 (MQ=255)
                                                                    cAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCatat                                                                               >  2:4119714/1‑150 (MQ=255)
                                                                     aaTGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATAtt                                                                              <  1:2128487/150‑1 (MQ=255)
                                                                       tGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGa                                                                            >  2:4634415/1‑150 (MQ=255)
                                                                        ggCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGaa                                                                           >  2:2440756/1‑150 (MQ=255)
                                                                              gaAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGc                                                                     <  1:2480551/150‑1 (MQ=255)
                                                                              gaAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGc                                                                     <  1:2480625/150‑1 (MQ=255)
                                                                               aaaTTTCCGGCCCAGTTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCa                                                                    <  1:4414344/150‑1 (MQ=255)
                                                                               aaaTTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCa                                                                    >  1:1045600/1‑150 (MQ=255)
                                                                                aaTTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCat                                                                   >  1:2788956/1‑150 (MQ=255)
                                                                                   ttCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatgat                                                                <  1:3561661/150‑1 (MQ=255)
                                                                                   ttCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatgat                                                                >  2:4104990/1‑150 (MQ=255)
                                                                                   ttCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatgat                                                                >  1:3311165/1‑150 (MQ=255)
                                                                                   ttCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatgat                                                                <  2:3079347/150‑1 (MQ=255)
                                                                                    tCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatgatg                                                               >  2:442940/1‑150 (MQ=255)
                                                                                     ccGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCAtgatga                                                              >  2:2074994/1‑150 (MQ=255)
                                                                                      cGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGAc                                                             <  1:506116/150‑1 (MQ=255)
                                                                                      cGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGAc                                                             <  1:1244459/150‑1 (MQ=255)
                                                                                       ggCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACg                                                            <  1:3957680/150‑1 (MQ=255)
                                                                                          ccAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCg                                                         >  1:3122986/1‑150 (MQ=255)
                                                                                          ccAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCg                                                         >  1:3123013/1‑150 (MQ=255)
                                                                                          ccAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCg                                                         <  1:3630471/150‑1 (MQ=255)
                                                                                             aTTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTc                                                      >  1:3457018/1‑150 (MQ=255)
                                                                                              ttGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCa                                                     <  1:1186043/150‑1 (MQ=255)
                                                                                               tgtgATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCAc                                                    <  2:3239749/150‑1 (MQ=255)
                                                                                                      aaaGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGGTGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGaa                                             >  2:88168/1‑150 (MQ=255)
                                                                                                       aaGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGaaa                                            <  1:2433348/150‑1 (MQ=255)
                                                                                                         ggACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAAcc                                          <  1:4262441/150‑1 (MQ=255)
                                                                                                         ggACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAAcc                                          <  2:1820710/150‑1 (MQ=255)
                                                                                                            cGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCt                                       <  1:1212050/150‑1 (MQ=255)
                                                                                                            cGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCt                                       <  1:622252/150‑1 (MQ=255)
                                                                                                             gTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTa                                      >  2:1211373/1‑150 (MQ=255)
                                                                                                              ttACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTAc                                     >  1:2309262/1‑150 (MQ=255)
                                                                                                              ttACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTAc                                     <  1:3797302/150‑1 (MQ=255)
                                                                                                                    tGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGcc                               >  2:4850081/1‑150 (MQ=255)
                                                                                                                    tGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGcc                               <  2:3520918/150‑1 (MQ=255)
                                                                                                                    tGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGcc                               <  1:2127242/150‑1 (MQ=255)
                                                                                                                    tGCCGGTGTTGGCGCAGAATACACTGATGCTCAGGATTTGCAGCGGTTTGATGCTCGAGGCGCAACGGCGGTGCCAGTGTTTATTGATGATAACCTTGATATTGAATCCTTCATGATGACGCCTGTCACTTTTGATACCGCTACCCTGcc                               <  2:3975731/150‑1 (MQ=255)
                                                                                                                     gCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCcg                              >  1:623292/1‑150 (MQ=255)
                                                                                                                     gCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCcg                              >  2:1359642/1‑150 (MQ=255)
                                                                                                                     gCCGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCcg                              <  1:1451348/150‑1 (MQ=255)
                                                                                                                      ccGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCcgc                             <  1:2338662/150‑1 (MQ=255)
                                                                                                                       cGGTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCcgcg                            <  1:1534881/150‑1 (MQ=255)
                                                                                                                        ggTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGATACCGCTACCCTGCcgcgc                           <  1:700340/150‑1 (MQ=255)
                                                                                                                        ggTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCcgcgc                           <  1:4194804/150‑1 (MQ=255)
                                                                                                                        ggTGTTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCcgcgc                           <  2:589090/150‑1 (MQ=255)
                                                                                                                          tgtTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCgg                         >  2:2430353/1‑150 (MQ=255)
                                                                                                                           gtTGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGc                        >  1:1508709/1‑150 (MQ=255)
                                                                                                                            ttGGCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGcc                       >  1:1866955/1‑150 (MQ=255)
                                                                                                                              ggCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTg                     <  2:1153766/150‑1 (MQ=255)
                                                                                                                              ggCGCAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACAGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTg                     <  1:858805/150‑1 (MQ=255)
                                                                                                                                cgcAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTgac                   <  1:4643746/150‑1 (MQ=255)
                                                                                                                                cgcAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTgac                   <  2:4480211/150‑1 (MQ=255)
                                                                                                                                cgcAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTgac                   >  1:13915/1‑150 (MQ=255)
                                                                                                                                cgcAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTgac                   <  2:1300057/150‑1 (MQ=255)
                                                                                                                                  cAGAATACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTgacga                 >  2:4762533/1‑150 (MQ=255)
                                                                                                                                     aaTACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCg              >  1:4009629/1‑150 (MQ=255)
                                                                                                                                      aTACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGt             >  2:1912285/1‑150 (MQ=255)
                                                                                                                                      aTACACTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGt             >  2:1049543/1‑150 (MQ=255)
                                                                                                                                         cacTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTAt          >  1:3788232/1‑150 (MQ=255)
                                                                                                                                           cTGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTAttt        >  2:265443/1‑150 (MQ=255)
                                                                                                                                            tGATGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTg       <  1:2952382/150‑1 (MQ=255)
                                                                                                                                              aTGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCg     <  1:3646518/150‑1 (MQ=255)
                                                                                                                                               tGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGa    >  1:301881/1‑150 (MQ=255)
                                                                                                                                               tGCTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGa    >  2:2327704/1‑150 (MQ=255)
                                                                                                                                                 cTCAGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGAcc  <  1:3403055/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTAGCCGTTTCCCGTGGCGACGCTGTTGCCGATTATATTATTGATAATGTCTCTATTCTCGACCTGATCAATGGCGGAGAAATTTCCGGCCCAATTGTGATTAAAGGACGTTACATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACC  >  CP009273/3837384‑3837678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑