Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,861,553 C→A 100% G373C (GGC→TGC)  yidR ← DUF3748 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,861,5530CA98.0% 940.6 / 5.9 293G373C (GGC→TGC) yidRDUF3748 family protein
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (147/139);  total (150/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 296 total aligned reads displayed.

ATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACA  >  CP009273/3861406‑3861699
                                                                                                                                                   |                                                                                                                                                  
aTGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTcgcagc                                                                                                                                                  <  2:2177945/150‑1 (MQ=255)
   ccTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCttt                                                                                                                                               <  1:4261768/150‑1 (MQ=255)
   ccTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCttt                                                                                                                                               >  2:3841472/1‑150 (MQ=255)
   ccTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCttt                                                                                                                                               >  2:2661872/1‑150 (MQ=255)
     tGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGATTTgc                                                                                                                                             <  1:1755574/150‑1 (MQ=255)
        tAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCa                                                                                                                                          >  2:1019740/1‑150 (MQ=255)
         aaCTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCAt                                                                                                                                         <  2:3795073/150‑1 (MQ=255)
         aaCTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCAt                                                                                                                                         <  2:3180069/150‑1 (MQ=255)
         aaCTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCAGCAGAAGGTGAATTGGCGAGGTGTTCGGTTAAATACTCAACCTCGCAGCTATGCGCAt                                                                                                                                         >  2:1519227/1‑150 (MQ=255)
              aTTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGca                                                                                                                                    >  2:3007718/1‑150 (MQ=255)
                 tAGCGATCAGTTTCGGTGATCCACAGCTGGCAGCCTTCCATCCACGCCAGCCACTGACAATCCGGCGAGAAGTCCACTGCGTCCGCAGATGGTGTATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACaa                                                                                                                                 <  2:2990261/150‑1 (MQ=255)
                  aGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAg                                                                                                                                <  1:3774627/150‑1 (MQ=255)
                    cGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCa                                                                                                                              >  1:2356937/1‑150 (MQ=255)
                    cGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCa                                                                                                                              <  1:3919478/150‑1 (MQ=255)
                     gATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCaa                                                                                                                             >  2:1084093/1‑150 (MQ=255)
                     gATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCaa                                                                                                                             >  1:3435248/1‑150 (MQ=255)
                      aTCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAAt                                                                                                                            <  2:4938907/150‑1 (MQ=255)
                      aTCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAAt                                                                                                                            >  2:3744505/1‑150 (MQ=255)
                       tCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAAtt                                                                                                                           >  1:2784594/1‑150 (MQ=255)
                        cAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTc                                                                                                                          <  1:958386/150‑1 (MQ=255)
                        cAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTc                                                                                                                          >  2:647722/1‑150 (MQ=255)
                         aGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCg                                                                                                                         >  2:1602455/1‑150 (MQ=255)
                            ttCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGAtt                                                                                                                      >  1:2385319/1‑150 (MQ=255)
                                              gccgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCaa                                                                                                    <  1:4912668/150‑1 (MQ=255)
                                                cgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAAcc                                                                                                  >  2:1381277/1‑150 (MQ=255)
                                                 gccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCa                                                                                                 <  2:5141626/150‑1 (MQ=255)
                                                  ccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCAt                                                                                                >  1:3562323/1‑150 (MQ=255)
                                                  ccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCAt                                                                                                >  1:2001259/1‑150 (MQ=255)
                                                  ccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAAGTCGAGTATCCAGCACCAAGCCCAGCCAt                                                                                                >  1:5073595/1‑150 (MQ=255)
                                                     tccatccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATtct                                                                                             <  1:3070101/150‑1 (MQ=255)
                                                      ccatccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATtctc                                                                                            <  2:2286000/150‑1 (MQ=255)
                                                      ccatccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATtctc                                                                                            <  1:1699848/150‑1 (MQ=255)
                                                               ccagccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGAt                                                                                   <  2:1679503/150‑1 (MQ=255)
                                                               ccagccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGAt                                                                                   <  1:3586480/150‑1 (MQ=255)
                                                                cagccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATg                                                                                  <  1:4726766/150‑1 (MQ=255)
                                                                 agccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGc                                                                                 <  2:183488/150‑1 (MQ=255)
                                                                    caCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAg                                                                              <  1:3637632/150‑1 (MQ=255)
                                                                    caCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGCTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAg                                                                              >  2:4776921/1‑150 (MQ=255)
                                                                      cTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGtt                                                                            <  1:1384247/150‑1 (MQ=255)
                                                                       tGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTa                                                                           <  2:860167/150‑1 (MQ=255)
                                                                        gACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTaa                                                                          >  1:4402175/1‑150 (MQ=255)
                                                                        gACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTaa                                                                          >  1:2220799/1‑150 (MQ=255)
                                                                          ccctCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAAt                                                                        <  1:4292078/147‑1 (MQ=255)
                                                                          ccATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAAt                                                                        <  1:3620821/150‑1 (MQ=255)
                                                                            aTCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGc                                                                      >  1:1094729/1‑150 (MQ=255)
                                                                              ccGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCAAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAg                                                                    <  1:3135509/150‑1 (MQ=255)
                                                                                ggCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGAc                                                                  <  1:973293/150‑1 (MQ=255)
                                                                                  cGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTg                                                                <  2:4455492/150‑1 (MQ=255)
                                                                                   gagaAGTCCACGGCGTCCGCAGTAGGTGAATTGGCGTGGTGTTCGGTTAACTCCTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAAGTCGATTATCCAGCACAAAGCCCTACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGa                                                               <  2:512564/150‑1 (MQ=255)
                                                                                   gagaAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGa                                                               <  2:3318323/150‑1 (MQ=255)
                                                                                   gagaAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGa                                                               <  2:109343/150‑1 (MQ=255)
                                                                                      aagaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAata                                                            >  2:4054058/1‑150 (MQ=255)
                                                                                      aagaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAata                                                            >  2:1926789/1‑150 (MQ=255)
                                                                                       agaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAatat                                                           <  1:844896/150‑1 (MQ=255)
                                                                                        gaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATc                                                          <  2:3562323/150‑1 (MQ=255)
                                                                                          ccACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCg                                                        <  1:4489513/150‑1 (MQ=255)
                                                                                          ccACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCg                                                        <  1:2625644/150‑1 (MQ=255)
                                                                                          ccACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCg                                                        >  1:2039753/1‑150 (MQ=255)
                                                                                            aCGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGtt                                                      >  2:2116882/1‑150 (MQ=255)
                                                                                                cGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGt                                                  >  1:2742371/1‑150 (MQ=255)
                                                                                                 gTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGtt                                                 >  2:1035146/1‑150 (MQ=255)
                                                                                                 gTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGtt                                                 >  2:1272754/1‑150 (MQ=255)
                                                                                                     gCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATgg                                             <  2:3939879/150‑1 (MQ=255)
                                                                                                      cAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATggg                                            <  1:666765/150‑1 (MQ=255)
                                                                                                      cAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATggg                                            >  1:4344236/1‑150 (MQ=255)
                                                                                                      cAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATggg                                            >  2:4395414/1‑150 (MQ=255)
                                                                                                       aGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGt                                           <  2:4662950/150‑1 (MQ=255)
                                                                                                        gAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGtt                                          >  2:4706715/1‑150 (MQ=255)
                                                                                                        gAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTGTGTTATGGGtt                                          >  2:284195/1‑150 (MQ=255)
                                                                                                         aaGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTa                                         >  1:2485329/1‑150 (MQ=255)
                                                                                                            gTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACt                                      <  2:536335/150‑1 (MQ=255)
                                                                                                             tGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTg                                     >  1:3261580/1‑150 (MQ=255)
                                                                                                             tGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTg                                     <  2:2380017/150‑1 (MQ=255)
                                                                                                               aaTTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGTCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGTGTTAACTGgc                                   <  2:3326032/150‑1 (MQ=255)
                                                                                                               aaTTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGgc                                   >  1:2926330/1‑150 (MQ=255)
                                                                                                               aaTTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGgc                                   >  2:2392492/1‑150 (MQ=255)
                                                                                                                aTTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGgcg                                  <  1:1064989/150‑1 (MQ=255)
                                                                                                                  tGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGcgcg                                >  2:4275046/1‑150 (MQ=255)
                                                                                                                  tGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGcgcg                                <  1:705543/150‑1 (MQ=255)
                                                                                                                  tGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGcgcg                                <  2:1243801/150‑1 (MQ=255)
                                                                                                                      gtggtgTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTc                            >  2:3663513/1‑150 (MQ=255)
                                                                                                                       tggtgTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTca                           <  1:2688428/150‑2 (MQ=255)
                                                                                                                        ggtgTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTcgc                          <  1:4187220/150‑1 (MQ=255)
                                                                                                                        ggtgTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTcgc                          >  1:1330529/1‑150 (MQ=255)
                                                                                                                         gtgtTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTcgcc                         <  2:3103965/150‑1 (MQ=255)
                                                                                                                           gtTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGACTATCCGTTTTGTTCTGGGTTAACTGGCGCGGCTcgccgc                       >  2:3477987/1‑150 (MQ=255)
                                                                                                                              cGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCt                    >  1:2292665/1‑150 (MQ=255)
                                                                                                                                 ttAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtgtg                 <  2:2356937/150‑1 (MQ=255)
                                                                                                                                    aaTACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCg              <  1:3543625/150‑1 (MQ=255)
                                                                                                                                    aaTACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCg              >  1:760026/1‑150 (MQ=255)
                                                                                                                                     aTACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCga             <  1:4017731/150‑1 (MQ=255)
                                                                                                                                     aTACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCga             <  2:4548502/150‑1 (MQ=255)
                                                                                                                                     aTACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCga             <  2:2742371/150‑1 (MQ=255)
                                                                                                                                      tACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCgag            >  2:5004948/1‑150 (MQ=255)
                                                                                                                                         tCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATa         >  1:1230329/1‑150 (MQ=255)
                                                                                                                                         tCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATa         <  2:959623/150‑1 (MQ=255)
                                                                                                                                            aCCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGc      >  1:4647416/1‑150 (MQ=255)
                                                                                                                                              cTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCca    >  1:583163/1‑150 (MQ=255)
                                                                                                                                               tCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCcac   >  2:2554316/1‑150 (MQ=255)
                                                                                                                                               tCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCcac   >  1:1426302/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACA  >  CP009273/3861406‑3861699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑