Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,871,008 G→T 17.7% intergenic (‑183/+57) yidB ← / ← gyrB DUF937 family protein/DNA gyrase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,871,0080GT17.7% 780.9 / 112.6 308intergenic (‑183/+57)yidB/gyrBDUF937 family protein/DNA gyrase, subunit B
Reads supporting (aligned to +/- strand):  ref base G (135/117);  new base T (27/29);  total (162/146)
Fisher's exact test for biased strand distribution p-value = 5.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

Only 100 of 310 total aligned reads displayed.

AAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATCAGCGTGGTGAACAACT  >  CP009273/3870859‑3871156
                                                                                                                                                     |                                                                                                                                                    
    attataGTTTTGCGGGTTGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGACGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATAttt                                                                                                                                                  <  1:766194/148‑1 (MQ=255)
       ataGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCg                                                                                                                                               >  1:3086359/1‑150 (MQ=255)
       ataGATGTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCg                                                                                                                                               <  1:3595283/150‑1 (MQ=255)
         aGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAATATCATGAAAATTCAATACATTGCAAGATTTTCGTa                                                                                                                                             <  2:1068808/150‑1 (MQ=255)
          gATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAg                                                                                                                                            >  2:3059766/1‑150 (MQ=255)
          gATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAg                                                                                                                                            <  1:4503738/150‑1 (MQ=255)
           aTTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAgg                                                                                                                                           >  1:155591/1‑150 (MQ=255)
            tttGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGc                                                                                                                                          <  1:2829188/150‑1 (MQ=255)
             ttGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGcc                                                                                                                                         >  1:898358/1‑150 (MQ=255)
              tGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCt                                                                                                                                        <  2:4536952/150‑1 (MQ=255)
                cGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGa                                                                                                                                      >  2:2271617/1‑150 (MQ=255)
                     tgatAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGc                                                                                                                                 >  2:577793/1‑150 (MQ=255)
                        tAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGAAAATTAAATACATAGCAAGATTTTCGTAGGCCGGATAAGCGTa                                                                                                                              >  1:2181862/1‑150 (MQ=255)
                         aaTTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAg                                                                                                                             >  1:4320737/1‑150 (MQ=255)
                          aTTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAgc                                                                                                                            <  2:2672830/150‑1 (MQ=255)
                          aTTGCGGAGTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAgc                                                                                                                            <  2:4905569/150‑1 (MQ=255)
                           ttGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAgcg                                                                                                                           <  1:1240161/150‑1 (MQ=255)
                               ggATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATc                                                                                                                       >  1:2470949/1‑150 (MQ=255)
                                 aTTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAg                                                                                                                     >  2:162098/1‑150 (MQ=255)
                                 aTTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAg                                                                                                                     >  2:2403120/1‑150 (MQ=255)
                                 aTTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAg                                                                                                                     <  2:3585438/150‑1 (MQ=255)
                                         aaTAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTc                                                                                                             >  1:1061683/1‑150 (MQ=255)
                                          aTAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCg                                                                                                            >  1:2598852/1‑150 (MQ=255)
                                            aaaaaGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCACGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCa                                                                                                          >  2:1113966/1‑150 (MQ=255)
                                            aaaaaGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCa                                                                                                          <  2:1987901/150‑1 (MQ=255)
                                                aGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGt                                                                                                      >  2:1712774/1‑150 (MQ=255)
                                                aGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGt                                                                                                      >  1:557529/1‑150 (MQ=255)
                                                 gggCGGATATGATCTCCGCCATTTTATTATAGCTTCTTGCCGGTTGCGGCGTGAACGCCTTATCCGGCCAAAAAAATAATTAAAATTCAATACATTGCATTATTTTCTTTGGCCTTATATGCGTAGCGCATCAGGCACGCTCGCATGGtt                                                                                                     <  1:2449146/150‑1 (MQ=21)
                                                 gggCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGtt                                                                                                     <  1:5105256/150‑1 (MQ=255)
                                                     ggAGATGATCTCCGCCCATTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCAACAAACTCATGAAAATTCAATCCATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAgcg                                                                                                 <  1:4633327/150‑1 (MQ=255)
                                                          tgatCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCAtt                                                                                            >  1:3287678/1‑150 (MQ=255)
                                                          tgatCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCAtt                                                                                            >  1:4955950/1‑150 (MQ=255)
                                                          tgatCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATCAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCAtt                                                                                            <  1:5036288/150‑1 (MQ=255)
                                                           gatCTCCGCCCTTTTCTTATCGCGTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAAGTCAAGACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTa                                                                                           <  2:3512084/150‑1 (MQ=255)
                                                           gatCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTa                                                                                           <  1:2773015/150‑1 (MQ=255)
                                                            atCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTaa                                                                                          >  1:3291212/1‑150 (MQ=255)
                                                             tctcCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTaaa                                                                                         >  2:1358472/1‑150 (MQ=255)
                                                              ctcCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAat                                                                                        <  2:1833902/150‑1 (MQ=255)
                                                              ctcCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAat                                                                                        >  1:2532018/1‑150 (MQ=255)
                                                               tcCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAata                                                                                       >  2:3957810/1‑150 (MQ=255)
                                                                 cGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATc                                                                                     <  2:3326678/150‑1 (MQ=255)
                                                                  gCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCg                                                                                    >  1:4882823/1‑150 (MQ=255)
                                                                  gCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCg                                                                                    >  1:1418947/1‑150 (MQ=255)
                                                                  gCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCg                                                                                    <  2:5027409/150‑1 (MQ=255)
                                                                  gCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTAACCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCCCGCTCGCATGGTTATCGCCATTAAATATCg                                                                                    >  1:3888878/1‑150 (MQ=255)
                                                                   cccTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGa                                                                                   >  2:142751/1‑150 (MQ=255)
                                                                   cccTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGa                                                                                   >  2:5119046/1‑150 (MQ=255)
                                                                   cccTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGa                                                                                   >  2:2359212/1‑150 (MQ=255)
                                                                    ccatttCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGat                                                                                  <  2:1949611/147‑1 (MQ=255)
                                                                    ccTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGat                                                                                  >  2:1238610/1‑150 (MQ=255)
                                                                        ttCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATCCATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTc                                                                              <  2:2985150/150‑1 (MQ=255)
                                                                        ttCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTc                                                                              <  2:3203247/150‑1 (MQ=255)
                                                                        ttCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTc                                                                              <  2:4668187/150‑1 (MQ=255)
                                                                        ttCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTc                                                                              >  1:3411035/1‑150 (MQ=255)
                                                                           ttATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAAGTCAATACATTGCACGATGTTCGTAGGCCTGATCAGCGTAGGGCAGCCGGCTCGCGCGCTGGGTTAGCGCCCGTCAATCTCGATATTcgcc                                                                           >  2:2056521/1‑150 (MQ=17)
                                                                             ataGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAATCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTcgccgc                                                                         >  2:2194137/1‑150 (MQ=255)
                                                                               aGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCtt                                                                       <  2:722042/150‑1 (MQ=255)
                                                                               aGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCtt                                                                       <  2:345128/150‑1 (MQ=255)
                                                                                gCTTCTTGCCGGATGCGGCTTGAACGCCTTATCCGGACTACAAAATCATTAAAATTAAATACATTGCAAGATTTTCGTTGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCttt                                                                      <  2:1781249/150‑1 (MQ=25)
                                                                                gCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCttt                                                                      >  1:175591/1‑150 (MQ=255)
                                                                                 cttcttGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTc                                                                     >  1:3350670/1‑150 (MQ=255)
                                                                                  ttcttGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCa                                                                    <  2:1274135/150‑1 (MQ=255)
                                                                                  ttcttGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCa                                                                    <  1:4112090/150‑1 (MQ=255)
                                                                                  ttcttGCCGGATGCGGCGTGAACGCATTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCa                                                                    <  1:3150313/150‑1 (MQ=255)
                                                                                    cttGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAgg                                                                  <  2:3788273/150‑1 (MQ=255)
                                                                                      tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGc                                                                >  2:2771696/1‑150 (MQ=255)
                                                                                       gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCg                                                               >  1:1899934/1‑150 (MQ=255)
                                                                                        ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGt                                                              >  1:2311238/1‑150 (MQ=255)
                                                                                         cGGATGCGGCGTGAACGCCTTATCCTGCCTACAAAATCATGAAAATTCAATCCATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATAGTCGCCGCTTTCAGGGCGtt                                                             <  1:5130466/150‑1 (MQ=255)
                                                                                         cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCCAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTACATCTCGATATTCGCCGCTTTCAGGGCGtt                                                             >  2:4325075/1‑150 (MQ=255)
                                                                                          ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTtc                                                            >  1:2722248/1‑150 (MQ=255)
                                                                                          ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTtc                                                            <  2:4132612/150‑1 (MQ=255)
                                                                                           gATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTtct                                                           <  1:2041136/150‑1 (MQ=255)
                                                                                                 gcgTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCaa                                                     <  2:2214700/150‑1 (MQ=255)
                                                                                                  cgTGAACGCCTTATCCGTCCTAAAAATTCATGAAAATTCAATCCATTGCAATATTTTCGTAGGCCTGATAAGCTTAGATCATCAGTCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAAt                                                    <  1:577793/150‑1 (MQ=255)
                                                                                                  cgTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAAt                                                    >  1:1802985/1‑150 (MQ=255)
                                                                                                   gTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATa                                                   <  1:1652025/150‑1 (MQ=255)
                                                                                                    tGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATaa                                                  >  2:319921/1‑150 (MQ=255)
                                                                                                        cGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGc                                              <  1:1869197/150‑1 (MQ=255)
                                                                                                         gCCTTATCCGGCCTACAAAATTAGGAAAAGTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAATTATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCa                                             <  1:3974111/150‑1 (MQ=255)
                                                                                                          ccTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACTCTCGCATGGTTAGCGCCATTCAATATCGATATTCTCCGCTTTCAGGGCGTTCTCTTCAATAAACGCAc                                            >  2:2948769/1‑150 (MQ=255)
                                                                                                          ccTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCAc                                            >  1:1008639/1‑150 (MQ=255)
                                                                                                          ccTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCAc                                            >  1:4198570/1‑150 (MQ=255)
                                                                                                           catATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTATCAGGGCGTTCTCTTCAATAAACGCACg                                           >  1:4246307/3‑150 (MQ=255)
                                                                                                              aTCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCGGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGgcg                                        >  1:823444/1‑150 (MQ=255)
                                                                                                                 cGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCgg                                     >  2:2502937/1‑150 (MQ=255)
                                                                                                                 cGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCgg                                     <  1:4148319/150‑1 (MQ=255)
                                                                                                                   gcatACAAAATCATGAAAATTCAATACATTGCAATATTTTCGTATGCATGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGtt                                   <  2:496896/147‑1 (MQ=255)
                                                                                                                        cAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACg                              <  2:2536404/150‑1 (MQ=255)
                                                                                                                        cAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACg                              >  1:1520871/1‑150 (MQ=255)
                                                                                                                         aaaaTCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATAGTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACgg                             <  2:3130470/150‑1 (MQ=255)
                                                                                                                           aaTCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCg                           <  2:3910512/150‑1 (MQ=255)
                                                                                                                           aaTCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCg                           <  1:4902678/150‑1 (MQ=255)
                                                                                                                            aTCATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGt                          <  1:368940/150‑1 (MQ=255)
                                                                                                                              cATGAAAATTCAATACATTGCAATATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCg                        >  2:889751/1‑150 (MQ=255)
                                                                                                                                 gAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGccc                     >  1:2226179/1‑150 (MQ=255)
                                                                                                                                  aaaaTTCAATACAGTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATAGTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCa                    <  2:4474494/150‑1 (MQ=255)
                                                                                                                                    aaTTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATc                  >  1:3043118/1‑150 (MQ=255)
                                                                                                                                         aaTACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATCAGCgt             <  1:1717376/150‑1 (MQ=255)
                                                                                                                                          aTACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATCAGCgtg            >  2:2249360/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATTAAATATCGATATTCGCCGCTTTCAGGGCGTTCTCTTCAATAAACGCACGGCGCGGTTCAACGGCGTCGCCCATCAGCGTGGTGAACAACT  >  CP009273/3870859‑3871156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑