Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,873,695 C→A 79.5% R296L (CGG→CTG)  recF ← gap repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,873,6950CA79.5% 537.9 / 177.4 297R296L (CGG→CTG) recFgap repair protein
Reads supporting (aligned to +/- strand):  ref base C (40/21);  new base A (123/113);  total (163/134)
Fisher's exact test for biased strand distribution p-value = 6.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 308 total aligned reads displayed.

TCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGG  >  CP009273/3873546‑3873842
                                                                                                                                                     |                                                                                                                                                   
   aaTTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACAcagcc                                                                                                                                                  >  2:3682952/1‑150 (MQ=255)
   aaTTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACAcagcc                                                                                                                                                  >  1:2457936/1‑150 (MQ=255)
    aTTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACAccgccg                                                                                                                                                 >  1:317481/1‑150 (MQ=255)
    aTTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACAcagccg                                                                                                                                                 >  1:1498151/1‑150 (MQ=255)
       ttCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGccgccc                                                                                                                                              <  1:3336252/150‑1 (MQ=255)
        tCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGccgcccg                                                                                                                                             >  1:3043516/1‑150 (MQ=255)
         cGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGccgcccgc                                                                                                                                            >  2:4706291/1‑150 (MQ=255)
          gTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCt                                                                                                                                           <  2:4937689/150‑1 (MQ=255)
            ccGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCttt                                                                                                                                         <  1:778891/150‑1 (MQ=255)
             cGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTc                                                                                                                                        >  2:4491548/1‑150 (MQ=255)
             cGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTc                                                                                                                                        >  1:2985151/1‑150 (MQ=255)
                     cTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGTCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGa                                                                                                                                >  2:4863919/1‑150 (MQ=255)
                       ataACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAgg                                                                                                                              <  1:3467277/150‑1 (MQ=255)
                       ataACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAgg                                                                                                                              <  1:4456235/150‑1 (MQ=255)
                       ataACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAgg                                                                                                                              >  2:1719150/1‑150 (MQ=255)
                        taACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGa                                                                                                                             >  1:2232795/1‑150 (MQ=255)
                        taACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGa                                                                                                                             >  2:4178003/1‑150 (MQ=255)
                         aaCGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGaa                                                                                                                            >  1:3731232/1‑150 (MQ=255)
                           cGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCACTTTCACGGGTGAGGAAct                                                                                                                          >  2:3123248/1‑150 (MQ=255)
                            gtgtTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAActc                                                                                                                         >  2:855117/1‑150 (MQ=255)
                             tgtTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAActct                                                                                                                        >  1:5149489/1‑150 (MQ=255)
                             tgtTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAActct                                                                                                                        <  1:4426194/150‑1 (MQ=255)
                              gtTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACtctc                                                                                                                       >  2:1244038/1‑150 (MQ=255)
                                 cAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCtt                                                                                                                    >  1:3888498/1‑150 (MQ=255)
                                 cAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCtt                                                                                                                    <  1:814918/150‑1 (MQ=255)
                                   gCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTgc                                                                                                                  >  2:3685217/1‑150 (MQ=255)
                                    cACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTgcg                                                                                                                 <  1:3568469/150‑1 (MQ=255)
                                     aCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTgcgc                                                                                                                >  1:3758325/1‑150 (MQ=255)
                                      cTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGcc                                                                                                               >  2:4107056/1‑150 (MQ=255)
                                      cTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGcc                                                                                                               <  2:755778/150‑1 (MQ=255)
                                       tGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCa                                                                                                              >  2:3032214/1‑150 (MQ=255)
                                       tGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCa                                                                                                              <  2:4403534/150‑1 (MQ=255)
                                        gATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCACGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGACCTCTCCTTGCGCCAg                                                                                                             >  2:2272392/1‑150 (MQ=255)
                                        gATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCTGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAg                                                                                                             >  2:4272610/1‑150 (MQ=255)
                                          tCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGAc                                                                                                           <  1:1211397/150‑1 (MQ=255)
                                          tCGCGCTGACAAAGACCTGTGATTGCGTCGCGTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGAc                                                                                                           >  2:347666/1‑150 (MQ=255)
                                           cgcgCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACg                                                                                                          <  2:1193192/150‑1 (MQ=255)
                                             cgcTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGtc                                                                                                        >  2:489714/1‑149 (MQ=255)
                                             cgcTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTa                                                                                                        >  2:3006495/1‑150 (MQ=255)
                                                tGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAgg                                                                                                     >  2:2911147/1‑150 (MQ=255)
                                                 gACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGgc                                                                                                    >  1:4192466/1‑150 (MQ=255)
                                                  aCAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTCAGgcg                                                                                                   >  2:824251/1‑150 (MQ=255)
                                                   cAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGgcgc                                                                                                  >  2:3247060/1‑150 (MQ=255)
                                                             tgATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAg                                                                                        <  1:1060182/150‑1 (MQ=255)
                                                                 tGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTa                                                                                    <  2:380502/150‑1 (MQ=255)
                                                                  gCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTaa                                                                                   <  2:2702156/150‑1 (MQ=255)
                                                                     tCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCAGTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCt                                                                                >  1:2613846/1‑150 (MQ=255)
                                                                       gCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGc                                                                              <  2:2503431/150‑1 (MQ=255)
                                                                       gCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGc                                                                              <  1:969999/150‑1 (MQ=255)
                                                                       gCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGc                                                                              <  2:1297538/150‑1 (MQ=255)
                                                                         ttttAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGccc                                                                            <  1:3664928/150‑1 (MQ=255)
                                                                          tttAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCa                                                                           <  1:2385169/150‑1 (MQ=255)
                                                                                cGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGAt                                                                     <  1:4126829/150‑1 (MQ=255)
                                                                                 ggCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATa                                                                    <  1:1822126/150‑1 (MQ=255)
                                                                                    tGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACCTCAACAGCTTAAGCTGCCCACGCGATAAgg                                                                 >  2:4021126/1‑150 (MQ=255)
                                                                                       cAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTAt                                                              >  2:1798257/1‑150 (MQ=255)
                                                                                       cAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTAt                                                              <  1:3274624/150‑1 (MQ=255)
                                                                                        aaGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATc                                                             >  1:2817610/1‑150 (MQ=255)
                                                                                          gcagcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCtt                                                           >  2:2829129/1‑150 (MQ=255)
                                                                                            agcCCGCGACGCTCATCATCAAGCTCAGAGTCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTcc                                                         <  1:2724825/150‑1 (MQ=255)
                                                                                            agcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTcc                                                         <  2:596102/150‑1 (MQ=255)
                                                                                               ccGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCACCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCAcc                                                      >  1:1940487/1‑150 (MQ=255)
                                                                                                  cgACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGgc                                                   <  1:4242074/150‑1 (MQ=255)
                                                                                                     cGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCa                                                >  2:3960885/1‑150 (MQ=255)
                                                                                                     cGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCa                                                >  2:1886330/1‑150 (MQ=255)
                                                                                                      gCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCAc                                               <  1:4615066/150‑1 (MQ=255)
                                                                                                        tcatcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCg                                             >  1:4793872/1‑150 (MQ=255)
                                                                                                          atcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCCACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTc                                           <  2:2396028/150‑1 (MQ=255)
                                                                                                             atcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGgc                                        >  1:331489/1‑150 (MQ=255)
                                                                                                              tcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGgcg                                       >  1:4941323/1‑150 (MQ=255)
                                                                                                                aaGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGcgcg                                     >  1:1680400/1‑150 (MQ=255)
                                                                                                                aaGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGcgcg                                     >  1:3508316/1‑150 (MQ=255)
                                                                                                                aaGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGcgcg                                     >  1:2130094/1‑150 (MQ=255)
                                                                                                                  gCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGaa                                   <  1:3854231/150‑1 (MQ=255)
                                                                                                                  gCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGaa                                   >  1:4881372/1‑150 (MQ=255)
                                                                                                                   cTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAAt                                  >  2:3337082/1‑150 (MQ=255)
                                                                                                                   cTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAAt                                  >  1:4654753/1‑150 (MQ=255)
                                                                                                                    tCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATg                                 <  1:4751497/150‑1 (MQ=255)
                                                                                                                     cAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGc                                >  2:2025445/1‑150 (MQ=255)
                                                                                                                      agagGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCg                               >  2:4328591/1‑150 (MQ=255)
                                                                                                                       gagGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGt                              >  1:3375398/1‑150 (MQ=255)
                                                                                                                        agGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTa                             >  2:1772068/1‑150 (MQ=255)
                                                                                                                           cAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGt                          <  2:4995816/150‑1 (MQ=255)
                                                                                                                            aaaaTCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTc                         >  2:4579112/1‑150 (MQ=255)
                                                                                                                            aaaaTCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTc                         >  2:2944560/1‑150 (MQ=255)
                                                                                                                            aaaaTCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTc                         >  1:36139/1‑150 (MQ=255)
                                                                                                                             aaaTCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTcc                        <  1:3753951/150‑1 (MQ=255)
                                                                                                                              aaTCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCg                       >  2:4075374/1‑150 (MQ=255)
                                                                                                                              aaTCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCg                       <  1:476368/150‑1 (MQ=255)
                                                                                                                               atcatcTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCGGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGc                      <  2:5136027/150‑1 (MQ=255)
                                                                                                                                tcatcTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCt                     <  1:2748795/150‑1 (MQ=255)
                                                                                                                                tcatcTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCt                     <  1:1311947/150‑1 (MQ=255)
                                                                                                                                    cTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTtgt                 >  1:2884587/1‑150 (MQ=255)
                                                                                                                                     tATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTtgtg                >  2:1347017/1‑150 (MQ=255)
                                                                                                                                      aTCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGc               <  1:1719150/150‑1 (MQ=255)
                                                                                                                                      aTCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGc               >  1:1915158/1‑150 (MQ=255)
                                                                                                                                       tCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCg              <  2:4142945/150‑1 (MQ=255)
                                                                                                                                        cAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCgg             <  2:1562753/150‑1 (MQ=255)
                                                                                                                                           gTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGcc          <  1:4978926/150‑1 (MQ=255)
                                                                                                                                              gagaCACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTg       >  2:1834896/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGG  >  CP009273/3873546‑3873842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑