BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,874,415	T→G	6.3%	Q56P (CAG→CCG)	recF ←	gap repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,874,415	0	T	G	6.3%	949.9 / 44.9	329	Q56P (CAG→CCG)	recF	gap repair protein
Reads supporting (aligned to +/- strand): ref base T (160/148); new base G (10/11); total (170/159)
Fisher's exact test for biased strand distribution p-value = 8.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 331 total aligned reads displayed.

CGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTTGCGGAAATCGCGGATCAA  >  CP009273/3874266‑3874563

   ncTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCa                                                                                                                                                   >  1:502055/2‑150 (MQ=255)

     ttATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCaaa                                                                                                                                                 >  2:3333832/1‑150 (MQ=255)

           ccGTCTGTACCGTCGATGCGGACTTTGCGGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGa                                                                                                                                           <  2:4148655/150‑1 (MQ=255)

           ccGTCTGTACCGTAGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCTCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGa                                                                                                                                           >  2:3099979/1‑150 (MQ=255)

              tCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAAc                                                                                                                                        <  1:3285846/150‑1 (MQ=255)

                  tACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGccc                                                                                                                                    <  2:2663916/150‑1 (MQ=255)

                       tCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCa                                                                                                                               <  1:1978876/150‑1 (MQ=255)

                          aTGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATgg                                                                                                                            >  2:171355/1‑150 (MQ=255)

                           tGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGc                                                                                                                           >  2:1196204/1‑150 (MQ=255)

                            gCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGcc                                                                                                                          <  2:610083/150‑1 (MQ=255)

                             cGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCg                                                                                                                         <  2:4618711/150‑1 (MQ=255)

                             cGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCg                                                                                                                         <  1:2365734/150‑1 (MQ=255)

                              ggACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCACTCTGCAAACTGCGAAACGCCCGACCATGGCCGa                                                                                                                        >  2:3369870/1‑150 (MQ=255)

                              ggACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGa                                                                                                                        >  2:4950916/1‑150 (MQ=255)

                              ggACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGa                                                                                                                        >  2:2881992/1‑150 (MQ=255)

                              ggACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCACCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGa                                                                                                                        >  2:370049/1‑150 (MQ=255)

                                aCTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCGGCAAACTGCGAAACGCCCGACCATGGCCGAGc                                                                                                                      <  2:2913310/150‑1 (MQ=255)

                                 cTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCg                                                                                                                     >  1:3469555/1‑150 (MQ=255)

                                   ttGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGta                                                                                                                   <  1:4241247/150‑1 (MQ=255)

                                       tGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGa                                                                                                               <  2:2463163/150‑1 (MQ=255)

                                         tCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATg                                                                                                             <  1:4518509/150‑1 (MQ=255)

                                           gCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGc                                                                                                           >  2:4273621/1‑150 (MQ=255)

                                             ccTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCtt                                                                                                         >  1:2424944/1‑150 (MQ=255)

                                                gTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCa                                                                                                      <  1:2128082/150‑1 (MQ=255)

                                                gTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCa                                                                                                      <  2:152005/150‑1 (MQ=255)

                                                  ttGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGc                                                                                                    >  1:4534245/1‑150 (MQ=255)

                                                   tGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCa                                                                                                   <  1:3699953/150‑1 (MQ=255)

                                                   tGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCa                                                                                                   >  2:4959050/1‑150 (MQ=255)

                                                       tttGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCt                                                                                               <  2:4734247/150‑1 (MQ=255)

                                                           gTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGtt                                                                                           >  1:2990501/1‑150 (MQ=255)

                                                            ttAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGttt                                                                                          <  2:2312162/150‑1 (MQ=255)

                                                              aaGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTg                                                                                        <  1:4656555/150‑1 (MQ=255)

                                                                  cAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCAc                                                                                    >  1:2557206/1‑150 (MQ=255)

                                                                  cAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCAc                                                                                    >  1:675315/1‑150 (MQ=255)

                                                                  cAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCAc                                                                                    >  1:1323740/1‑150 (MQ=255)

                                                                    aTCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTg                                                                                  >  1:1198482/1‑150 (MQ=255)

                                                                     tCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGc                                                                                 >  1:599448/1‑150 (MQ=255)

                                                                     tCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGc                                                                                 <  2:187219/150‑1 (MQ=255)

                                                                      cGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTCTAGATGGCTTCCAGCACGCTGGGTGTGCCACTGcc                                                                                >  2:3003915/1‑150 (MQ=255)

                                                                      cGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGcc                                                                                <  1:4734046/150‑1 (MQ=255)

                                                                      cGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGcc                                                                                <  1:399342/150‑1 (MQ=255)

                                                                       gCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCg                                                                               <  2:468221/150‑1 (MQ=255)

                                                                         tGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGtt                                                                             <  1:4871634/150‑1 (MQ=255)

                                                                         tGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGtt                                                                             >  2:1599977/1‑150 (MQ=255)

                                                                         tGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCGGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGtt                                                                             <  1:1631026/150‑1 (MQ=255)

                                                                            ctcGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGc                                                                          >  1:4347501/1‑150 (MQ=255)

                                                                             tcGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCa                                                                         >  1:3214252/1‑150 (MQ=255)

                                                                                tgcTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCt                                                                      >  1:4052195/2‑150 (MQ=255)

                                                                                tgcTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCt                                                                      >  2:2223345/2‑150 (MQ=255)

                                                                                cgcTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCt                                                                      >  2:547475/1‑150 (MQ=255)

                                                                                cgcTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCt                                                                      >  2:3454134/1‑150 (MQ=255)

                                                                                cgcTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCt                                                                      >  1:1351325/1‑150 (MQ=255)

                                                                                 gcTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTa                                                                     <  1:1818795/150‑1 (MQ=255)

                                                                                  ctctTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTAc                                                                    <  2:3868164/150‑1 (MQ=255)

                                                                                   tctTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTAcc                                                                   <  2:404737/150‑1 (MQ=255)

                                                                                   tctTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTAcc                                                                   >  2:4777024/1‑150 (MQ=255)

                                                                                   tctTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTAcc                                                                   >  1:490870/1‑150 (MQ=255)

                                                                                     ttCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAg                                                                 >  2:5079100/1‑150 (MQ=255)

                                                                                     ttCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCAAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAg                                                                 >  2:3141505/1‑150 (MQ=255)

                                                                                        gCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGaaa                                                              >  2:2511985/1‑150 (MQ=255)

                                                                                        gCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACAGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGaaa                                                              >  1:1019726/1‑150 (MQ=255)

                                                                                         cccTGTACTCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCGGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAg                                                             <  2:1021014/150‑1 (MQ=255)

                                                                                         cccTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAg                                                             >  2:4368521/1‑150 (MQ=255)

                                                                                              tAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTaaa                                                        >  1:169546/1‑150 (MQ=255)

                                                                                                aTCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGc                                                      >  2:4906111/1‑150 (MQ=255)

                                                                                                  cGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCg                                                    <  1:789326/150‑1 (MQ=255)

                                                                                                    cccGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCggg                                                  >  1:2668963/1‑150 (MQ=255)

                                                                                                    cccGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCGGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCggg                                                  >  2:1246041/1‑150 (MQ=255)

                                                                                                     ccGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGga                                                 >  1:5156052/1‑150 (MQ=255)

                                                                                                      cGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGgag                                                >  2:237522/1‑150 (MQ=255)

                                                                                                          gagaACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATaa                                            <  2:2689045/150‑1 (MQ=255)

                                                                                                          gagaACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATaa                                            <  2:2252252/150‑1 (MQ=255)

                                                                                                           agaACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAg                                           <  1:359495/150‑1 (MQ=255)

                                                                                                              aCAAACGCCTCCTGCTGATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCAGAGCGTATAGATGGCTTCCAGCAAGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATATGGCg                                        <  2:4106894/150‑1 (MQ=255)

                                                                                                              aCAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACGGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCg                                        <  2:1362595/150‑1 (MQ=255)

                                                                                                               cAAACGCCTCCTGCTCAGGGCGAATGACGCGACCAATCGGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTGTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCga                                       <  2:2802161/150‑1 (MQ=255)

                                                                                                                aaaCGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCgag                                      >  1:3940421/1‑150 (MQ=255)

                                                                                                                aaaCGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCgag                                      >  1:3801537/1‑150 (MQ=255)

                                                                                                                aaaCGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCgag                                      <  1:2942389/150‑1 (MQ=255)

                                                                                                                aaaCGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCgag                                      >  1:1360776/1‑150 (MQ=255)

                                                                                                                            cTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTc                          >  2:4214471/1‑150 (MQ=255)

                                                                                                                            cTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTc                          <  2:4035360/150‑1 (MQ=255)

                                                                                                                            cTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTc                          >  1:3141231/1‑150 (MQ=255)

                                                                                                                             tCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCa                         >  2:4418436/1‑150 (MQ=255)

                                                                                                                                  gCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGtt                    <  2:1748218/150‑1 (MQ=255)

                                                                                                                                    gAATGACGCGACCAATCGGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTTGc                  >  2:4484896/1‑150 (MQ=255)

                                                                                                                                    gAATGACGCGACCAATCGGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTTGc                  >  1:2183062/1‑150 (MQ=255)

                                                                                                                                     aaTGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTTGCg                 <  2:3895646/150‑1 (MQ=255)

                                                                                                                                     aaTGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTTGCg                 >  2:3251228/1‑150 (MQ=255)

                                                                                                                                      aTGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTTGCgg                <  2:5110789/150‑1 (MQ=255)

                                                                                                                                       tGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTTGCGGa               <  2:2404508/150‑1 (MQ=255)

                                                                                                                                        gACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATCAGGCGAGATCCGCGGTTTCAATGTTGCGGaa              >  2:94147/1‑150 (MQ=255)

                                                                                                                                          cgcgACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTTGCGGAAAt            <  1:2075232/150‑1 (MQ=255)

                                                                                                                                            cgACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTTGCGGAAATcg          <  1:2788688/150‑1 (MQ=255)

                                                                                                                                            cgACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTTGCGGAAATcg          <  1:4777024/150‑1 (MQ=255)

                                                                                                                                             gACCAATCGGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTTGCGGAAATcgc         >  2:737275/1‑150 (MQ=255)

                                                                                                                                               ccAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTTGCGGAAATCGCgg       <  2:3498441/150‑1 (MQ=255)

                                                                                                                                                 aaTCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTTGCGGAAATCGCGGAt     <  1:1879659/150‑1 (MQ=255)

                                                                                                                                                 aaTCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTTGCGGAAATCGCGGAt     <  1:2824935/150‑1 (MQ=255)

                                                                                                                                                 aaTCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAACTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTTGCGGAAATCGCGGAt     <  1:3872412/150‑1 (MQ=255)

CGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTTGCGGAAATCGCGGATCAA  >  CP009273/3874266‑3874563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`