Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,901,415 C→A 100% P104P (CCG→CCT)  pstB ← phosphate transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,901,4150CA98.7% 982.2 / 2.2 299P104P (CCG→CCT) pstBphosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (170/124);  total (172/127)
Fisher's exact test for biased strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 300 total aligned reads displayed.

AGAGTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGT  >  CP009273/3901268‑3901564
                                                                                                                                                   |                                                                                                                                                     
agagTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAgg                                                                                                                                                     <  2:3120655/150‑1 (MQ=255)
agagTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAgg                                                                                                                                                     >  2:4011100/1‑150 (MQ=255)
 gagTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGa                                                                                                                                                    <  2:3963498/150‑1 (MQ=255)
   gTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGaaa                                                                                                                                                  >  2:2924707/1‑150 (MQ=255)
       ccGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGc                                                                                                                                              >  2:142866/1‑150 (MQ=255)
       ccGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGc                                                                                                                                              >  1:415810/1‑150 (MQ=255)
       ccGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGc                                                                                                                                              >  2:4646996/1‑150 (MQ=255)
        cGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCg                                                                                                                                             >  2:4845944/1‑150 (MQ=255)
           tctGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCg                                                                                                                                          >  2:565901/1‑150 (MQ=255)
           tctGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCg                                                                                                                                          >  2:1023537/1‑150 (MQ=255)
              ggTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGtt                                                                                                                                       >  2:1659303/1‑150 (MQ=255)
              ggTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGtt                                                                                                                                       >  1:1796725/1‑150 (MQ=255)
                 taaaTTTATCTTTTGTTTAGTTCCACAATGCGGCTTTGGTCAATGCCAACTTCACTAGCTCGTCCATGACTGCACGGGAGAGCTTCTCAAACAGACGAACTCAAAAAGCGATGTTGTCGTATATGGACATAGGAAACGGCGTCGGTTTCt                                                                                                                                    >  2:712122/3‑150 (MQ=255)
                   aaTTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCATGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTgg                                                                                                                                  >  2:318776/1‑150 (MQ=255)
                   aaTTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTgg                                                                                                                                  >  2:1303994/1‑150 (MQ=255)
                     tttATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGaa                                                                                                                                >  1:3744344/1‑150 (MQ=255)
                        aTCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAacac                                                                                                                             <  2:4948105/150‑1 (MQ=255)
                         tCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACAcc                                                                                                                            >  1:2866639/1‑150 (MQ=255)
                          cTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCa                                                                                                                           >  2:2638478/1‑150 (MQ=255)
                           tttGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCAt                                                                                                                          >  1:1478925/1‑150 (MQ=255)
                            ttGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATg                                                                                                                         >  1:3078199/1‑150 (MQ=255)
                             tGGTTTCGTTCCACAATGCGGCTTTGGTCAAGGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGc                                                                                                                        <  1:1009958/150‑1 (MQ=255)
                               gTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGccc                                                                                                                      <  1:4941709/150‑1 (MQ=255)
                                tttCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCa                                                                                                                     >  2:2079113/1‑150 (MQ=255)
                                  tCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACt                                                                                                                   >  1:4998842/1‑150 (MQ=255)
                                    gTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACttt                                                                                                                 >  2:1181792/1‑150 (MQ=255)
                                        cacaATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcgca                                                                                                             >  2:2689062/1‑150 (MQ=255)
                                          caATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcgcacg                                                                                                           >  2:3834496/1‑150 (MQ=255)
                                             tGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACgcag                                                                                                        <  2:3690659/150‑1 (MQ=255)
                                               cGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACgcagca                                                                                                      >  1:1650108/1‑150 (MQ=255)
                                                  cTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTg                                                                                                   >  2:4457087/1‑150 (MQ=255)
                                                    ttGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCg                                                                                                 >  1:2365363/1‑150 (MQ=255)
                                                     tGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGa                                                                                                >  1:3298776/1‑150 (MQ=255)
                                                        tCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGGACACCATGCCCACTTTCGCACGCAGCAGTGCGatat                                                                                             <  2:1489224/150‑1 (MQ=255)
                                                        tCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGatat                                                                                             <  2:1891842/150‑1 (MQ=255)
                                                            tGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTg                                                                                         <  1:3312298/150‑1 (MQ=255)
                                                            tGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTg                                                                                         >  1:4645313/1‑150 (MQ=255)
                                                            tGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTg                                                                                         <  2:3747219/150‑1 (MQ=255)
                                                            tGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTg                                                                                         >  1:3904480/1‑150 (MQ=255)
                                                            tGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTg                                                                                         <  1:1256553/150‑1 (MQ=255)
                                                              cccACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTgag                                                                                       >  1:1107592/1‑150 (MQ=255)
                                                              cccACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTgag                                                                                       <  1:2780380/150‑1 (MQ=255)
                                                              cccACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTgag                                                                                       >  1:1438345/1‑150 (MQ=255)
                                                                 aCTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGt                                                                                    >  1:2313459/1‑150 (MQ=255)
                                                                 aCTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGt                                                                                    <  1:1880388/150‑1 (MQ=255)
                                                                        gcgcTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAg                                                                             <  2:4544319/150‑1 (MQ=255)
                                                                          gcTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGa                                                                           <  1:521496/150‑1 (MQ=255)
                                                                           cTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGNCGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAt                                                                          >  1:2677437/1‑150 (MQ=255)
                                                                           cTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAt                                                                          >  1:641735/1‑150 (MQ=255)
                                                                           cTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAt                                                                          >  1:3160544/1‑150 (MQ=255)
                                                                             cGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgt                                                                        >  1:803550/1‑150 (MQ=255)
                                                                             cGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgt                                                                        >  2:2462645/1‑150 (MQ=255)
                                                                              gTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgtt                                                                       <  2:2237339/150‑1 (MQ=255)
                                                                              gTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgtt                                                                       >  2:4989841/1‑150 (MQ=255)
                                                                                   tGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGc                                                                  >  2:1630232/1‑150 (MQ=255)
                                                                                      cGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCAGGCCCACTTTCGCACGCAACAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCAt                                                               <  2:802345/150‑1 (MQ=255)
                                                                                       ggCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATc                                                              >  1:2058547/1‑150 (MQ=255)
                                                                                        gCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCa                                                             <  2:1769520/150‑1 (MQ=255)
                                                                                              gagaGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTGTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGa                                                       >  2:2504615/1‑150 (MQ=255)
                                                                                                 agCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAtt                                                    <  2:2322292/150‑1 (MQ=255)
                                                                                                   cTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTc                                                  >  1:2748742/1‑150 (MQ=255)
                                                                                                   cTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTc                                                  <  2:532336/150‑1 (MQ=255)
                                                                                                      ctcAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAcc                                               >  1:4730083/1‑150 (MQ=255)
                                                                                                         aaaCAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTc                                            >  2:1987868/1‑150 (MQ=255)
                                                                                                         aaaCAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTc                                            >  2:2829511/1‑150 (MQ=255)
                                                                                                         aaaCAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTc                                            >  2:235888/1‑150 (MQ=255)
                                                                                                         aaaCAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTc                                            <  2:3539642/150‑1 (MQ=255)
                                                                                                            cAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGc                                         <  1:4390210/150‑1 (MQ=255)
                                                                                                              gACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCAc                                       <  1:2127718/150‑1 (MQ=255)
                                                                                                                 gAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgct                                    >  1:573668/1‑150 (MQ=255)
                                                                                                                 gAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgct                                    <  1:3507711/150‑1 (MQ=255)
                                                                                                                  aaCGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgctg                                   <  1:951119/150‑1 (MQ=255)
                                                                                                                     gCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTc                                <  1:4412482/150‑1 (MQ=255)
                                                                                                                     gCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTc                                >  1:1619574/1‑150 (MQ=255)
                                                                                                                     gCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTc                                >  2:4205152/1‑150 (MQ=255)
                                                                                                                     gCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTc                                <  1:2275851/150‑1 (MQ=255)
                                                                                                                       cAAAAGCGCTGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCGGCTCCg                              <  1:2224662/150‑1 (MQ=255)
                                                                                                                       cAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCg                              >  1:1926285/1‑150 (MQ=255)
                                                                                                                       cAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCg                              >  2:3153014/1‑150 (MQ=255)
                                                                                                                       cAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCg                              >  1:4813701/1‑150 (MQ=255)
                                                                                                                         aaaGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCggg                            <  1:2582317/150‑1 (MQ=255)
                                                                                                                           aGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTa                          >  1:3639968/1‑150 (MQ=255)
                                                                                                                           aGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTa                          >  1:2095590/1‑150 (MQ=255)
                                                                                                                           aGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTa                          <  1:2473671/150‑1 (MQ=255)
                                                                                                                            gCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTAc                         >  1:1322740/1‑150 (MQ=255)
                                                                                                                            gCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTAc                         >  1:5119850/1‑150 (MQ=255)
                                                                                                                            gCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTAc                         >  2:299749/1‑150 (MQ=255)
                                                                                                                            gCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTAc                         <  2:2775926/150‑1 (MQ=255)
                                                                                                                             cGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTCCa                        >  1:3420133/1‑150 (MQ=255)
                                                                                                                             cGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACa                        <  2:3349578/150‑1 (MQ=255)
                                                                                                                              gATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAg                       >  2:4393164/1‑150 (MQ=255)
                                                                                                                              gATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCAAGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTTGTCAGTATGTTGTCGCCATCAAGCAGAATTTCACCTTCAGCACGCTGCTCCGGGTACAg                       >  2:428221/1‑150 (MQ=255)
                                                                                                                                tgttgtCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGtt                     <  1:4565633/150‑1 (MQ=255)
                                                                                                                                     tCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAAc                <  1:3438296/150‑1 (MQ=255)
                                                                                                                                       gTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAt              <  2:217115/150‑1 (MQ=255)
                                                                                                                                         aGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAttt            <  1:2924707/150‑1 (MQ=255)
                                                                                                                                            tGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttgt         <  1:3735872/150‑1 (MQ=255)
                                                                                                                                             ggACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttgtt        >  1:4619179/1‑150 (MQ=255)
                                                                                                                                             ggACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttgtt        >  1:4870324/1‑150 (MQ=255)
                                                                                                                                                cATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGaa     >  2:4056762/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AGAGTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGT  >  CP009273/3901268‑3901564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑