| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,906,384 | G→T | 100% | I215I (ATC→ATA) | glmS ← | L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,906,384 | 0 | G | T | 98.4% | 1004.1 / 3.9 | 311 | I215I (ATC→ATA) | glmS | L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (2/3); new base T (187/119); total (189/122) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 311 total aligned reads displayed.
GATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCG > CP009273/3906237‑3906529 | gATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTAt > 2:3218980/1‑150 (MQ=255) aTCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTAtt > 1:4083956/1‑150 (MQ=255) aTCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTAtt > 2:1708986/1‑150 (MQ=255) cGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAat < 1:1752133/150‑1 (MQ=255) ggCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAata < 1:4051226/150‑1 (MQ=255) ggCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAata < 1:4737202/150‑1 (MQ=255) cTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATc > 2:2038155/1‑150 (MQ=255) gTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGc > 1:1693601/1‑150 (MQ=255) gTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGACTTTATCGCCCGCGTCATATTGCAGATTGGATTAGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATAGc > 1:226810/1‑150 (MQ=255) tAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCctct > 2:85359/1‑150 (MQ=255) tAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCctct < 1:4207740/150‑1 (MQ=255) gATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTc < 1:3091875/150‑1 (MQ=255) gATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTc < 1:1682299/150‑1 (MQ=255) aTCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCa > 2:914532/1‑150 (MQ=255) aTCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCa > 2:3046011/1‑150 (MQ=255) aTCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCa < 1:4358647/150‑1 (MQ=255) aTCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCa < 2:1503370/150‑1 (MQ=255) tctcTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCaa > 1:4509932/1‑150 (MQ=255) ctTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGa > 1:5127440/1‑150 (MQ=255) tGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATa < 1:4423798/150‑1 (MQ=255) gCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATaa < 1:836076/150‑1 (MQ=255) gTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCg < 1:2492714/150‑1 (MQ=255) gTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCg < 1:2189260/150‑1 (MQ=255) tAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCACTATCGCCCTCTTCAAGGAAGATAAAGCGa > 2:2880192/1‑150 (MQ=255) tAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGa > 1:4858428/1‑150 (MQ=255) tAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGa > 2:5080983/1‑150 (MQ=255) gTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACg < 1:2006135/150‑1 (MQ=255) tGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACgg > 1:2906679/1‑150 (MQ=255) aCGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGt > 1:2022499/1‑150 (MQ=255) cGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTc > 1:2884094/1‑150 (MQ=255) ggTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCa > 1:848975/1‑150 (MQ=255) tAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCAcc > 1:3851621/1‑150 (MQ=255) aaaTGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCg > 1:1047043/1‑150 (MQ=255) aaTGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCgg > 1:1307330/1‑150 (MQ=255) tGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCa > 1:97673/1‑150 (MQ=255) gCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCaa > 1:3728942/1‑150 (MQ=255) ccTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAAc > 2:2773091/1‑150 (MQ=255) cTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACa > 2:1637054/1‑150 (MQ=255) tATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAgcg > 2:4347949/1‑150 (MQ=255) cGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCa < 2:420802/150‑1 (MQ=255) gCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAg < 2:405781/150‑1 (MQ=255) gCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAg > 1:4648716/1‑150 (MQ=255) cccGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGc > 2:3099503/1‑150 (MQ=255) ccGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCt > 2:2625069/1‑150 (MQ=255) ccGCGTCATATGGCAGTTTGTATGCGATATCCTGCCGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGACCGGCGAGTTATTTCCGCAATCTCGCCCTCTTCAAGGACGATAACGCGACGGGTCACCGGCAACAGCGCCAGCt < 1:5075463/150‑1 (MQ=255) gcgTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTgg > 1:3502757/1‑150 (MQ=255) cgTCATATTGCAGATTGGATTAGATATACAGACTTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTACGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGt > 2:1153010/1‑150 (MQ=255) tCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGTCAACAGCGCCAGCTGGTCa < 2:1518596/150‑1 (MQ=255) tCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCa < 2:637226/150‑1 (MQ=255) cATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAg > 1:713127/1‑150 (MQ=255) cATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAg > 1:879462/1‑150 (MQ=255) atatTGCATATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGTTTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGa < 2:3488396/150‑1 (MQ=255) tatTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGaa < 1:3427842/150‑1 (MQ=255) atTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAg > 1:2953961/1‑150 (MQ=255) cAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGAt < 2:4887107/150‑1 (MQ=255) cAGATTGGATTCGATATCCTGACGTTGTCCTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGAt < 2:543776/150‑1 (MQ=255) gATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATaa > 1:1179844/1‑150 (MQ=255) aTTCTATAGCCTGACTTTTTACTTCCGCGCCATTTTTATCTAATTTTTTTACCGAACGGCGAGTTATTTCCGAAATATCGCCCTCTTCAAGTAATATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtttt < 1:2326997/150‑1 (MQ=255) aTTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtttt > 1:3188196/1‑150 (MQ=255) ttttttttCCTGACGTTTTAGTTCCGCTCAAGTTTTTTCTATTTTGTTTACGTAACGGCGTTTTTTTTCCGAAATATCGCACTATTCACGTAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTc < 1:2647322/143‑1 (MQ=255) gATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGcc > 1:1578576/1‑150 (MQ=255) atatCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGccc > 1:3217116/1‑150 (MQ=255) tatCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCa > 1:3121064/1‑150 (MQ=255) ccTGAGGTTTTACTTCCGCGCCTGTTTTTTCTAAGTTGTTTACCGACGGGCGTTTTTTTACCGCAATATCGCCCTCTTCATGTAATATAACGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATcc < 1:458662/150‑1 (MQ=255) ccTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATcc < 1:2576918/150‑1 (MQ=255) cttactttttAATTCCTCGCCTGTTTTATCTAATATTTTTACCGAACGGCGATTTATTTCCGAAATATCGCCCTCTTAAAGTAATATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATccc < 1:5052495/144‑1 (MQ=255) cTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATccc < 2:1912597/150‑1 (MQ=255) tGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATcccc > 1:4984675/1‑150 (MQ=255) gACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCa > 2:720950/1‑150 (MQ=255) aCGTTTTACTTCCGCGCCTGTTTTATCTAAGTTTTTTACCGAACGGCGTGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAg < 1:3313197/150‑1 (MQ=255) ttttACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCTATAAAGTTTTCGCCCATCCCCAGGcc > 2:306910/1‑150 (MQ=255) tttACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCa < 2:1674753/150‑1 (MQ=255) ttCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCAc > 2:1927693/1‑150 (MQ=255) tCCGCGCCAGTTTTATCGATGTTTTTTACCGACCGGCGTGTTTTTTCCGAAATATCGCACTCTTCAAGGATTATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCAcc < 1:4264097/150‑1 (MQ=255) gcgcCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGc > 2:1182590/1‑150 (MQ=255) gcgcCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGc > 2:5021755/1‑150 (MQ=255) gcCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCgg > 2:2282431/1‑150 (MQ=255) ccAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGa > 2:3773596/1‑150 (MQ=255) ttttATCGATTTTGTTTACCGAACGGCGTGTTATTCCCGCAATATCGCCCTCTTCAAGTAACATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTAc < 1:2690766/150‑1 (MQ=255) tttATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTAcc > 2:1028723/1‑150 (MQ=255) tATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAg > 2:3970954/1‑150 (MQ=255) gAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACg > 2:1685166/1‑150 (MQ=255) gAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACg > 1:1428699/1‑150 (MQ=255) aaGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGt > 1:2549702/1‑150 (MQ=255) gATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCTATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgc < 1:4228754/150‑1 (MQ=255) aTGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAATGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgcc > 2:2846597/1‑150 (MQ=255) aTGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgcc > 2:3450122/1‑150 (MQ=255) tGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgccg > 1:2099869/1‑150 (MQ=255) tGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgccg < 2:4121338/150‑1 (MQ=255) tGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgccg < 2:567980/150‑1 (MQ=255) tttACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgccgcc < 2:2573128/150‑1 (MQ=255) ttACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCca > 2:4566649/1‑150 (MQ=255) tACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCcag > 1:3562869/1‑150 (MQ=255) aCCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCcagc > 2:3347450/1‑150 (MQ=255) gAACGGCGATTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAgg < 2:2472245/150‑1 (MQ=255) aaCGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAggg > 2:4672963/1‑150 (MQ=255) tggCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTa > 2:3351808/2‑150 (MQ=255) ggCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCTATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTAt > 1:1871600/1‑150 (MQ=255) cGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATcc > 1:1894519/1‑150 (MQ=255) cGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATcc < 2:2934117/150‑1 (MQ=255) | GATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCG > CP009273/3906237‑3906529 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |