Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,910,416 C→A 100% R107L (CGT→CTT)  atpD ← F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,910,4160CA98.2% 922.0 / 3.1 289R107L (CGT→CTT) atpDF1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base A (147/135);  total (152/137)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 289 total aligned reads displayed.

TTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAA  >  CP009273/3910269‑3910562
                                                                                                                                                   |                                                                                                                                                  
ttACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGc                                                                                                                                                  <  1:3183741/150‑1 (MQ=255)
ttACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGc                                                                                                                                                  <  1:2243852/150‑1 (MQ=255)
 tACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGct                                                                                                                                                 >  1:703241/1‑150 (MQ=255)
   ccTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGctct                                                                                                                                               <  1:2304787/150‑1 (MQ=255)
    ctccacCCGCTCCACCTAACAGACCAACTTTAGATCCCCTATCGATCGGACACATCAGGTCTATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGTATCGCCCAAAGCtctt                                                                                                                                              <  1:435979/147‑1 (MQ=255)
    cTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCtctt                                                                                                                                              <  1:3299410/150‑1 (MQ=255)
     gacccccGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACCCATCAGGGCGAGAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCtcttc                                                                                                                                             <  2:2634572/146‑1 (MQ=255)
        acCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCttcttc                                                                                                                                          <  1:2576823/150‑1 (MQ=255)
        acCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCttcttc                                                                                                                                          >  1:3524005/1‑150 (MQ=255)
         cccGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCa                                                                                                                                         <  1:2883029/150‑1 (MQ=255)
         cccGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATAGCCCAAAGCTCTTCTTCa                                                                                                                                         >  2:2065032/1‑150 (MQ=255)
           cGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCTGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCAcc                                                                                                                                       <  2:4667992/150‑1 (MQ=255)
           cGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCAcc                                                                                                                                       <  1:5157730/150‑1 (MQ=255)
            gCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCg                                                                                                                                      >  2:4479110/1‑150 (MQ=255)
             caccacCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGa                                                                                                                                     <  2:1729028/150‑1 (MQ=255)
                caccGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGAtct                                                                                                                                  <  2:3640014/150‑1 (MQ=255)
                 accGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGAtctc                                                                                                                                 >  1:4972533/1‑150 (MQ=255)
                   cGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGc                                                                                                                               >  1:4276045/1‑150 (MQ=255)
                     aaCAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCt                                                                                                                             >  2:1294309/1‑150 (MQ=255)
                     aaCAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCt                                                                                                                             >  2:798223/1‑150 (MQ=255)
                       cAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCttt                                                                                                                           >  1:1048393/1‑150 (MQ=255)
                       cAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCttt                                                                                                                           <  1:1740532/150‑1 (MQ=255)
                         gACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAGCTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCa                                                                                                                         >  1:3220799/1‑150 (MQ=255)
                          aCCAACTTTACCGCGCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCAt                                                                                                                        <  1:442361/150‑1 (MQ=255)
                          aCCAACTTTACCGCCCTTAGCGAACTGACACATCAGGTCGATAACTTTGATACCGGTTTACAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCAt                                                                                                                        <  2:1455233/150‑1 (MQ=255)
                                tttACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGAc                                                                                                                  >  2:2715400/1‑150 (MQ=255)
                                tttACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTAc                                                                                                                  <  1:4695769/150‑1 (MQ=255)
                                tttACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTAc                                                                                                                  <  1:1823704/150‑1 (MQ=255)
                                 ttACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTAcc                                                                                                                 <  2:2121598/150‑1 (MQ=255)
                                     cGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGtt                                                                                                             <  1:3746745/150‑1 (MQ=255)
                                           tAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCa                                                                                                       >  1:661799/1‑150 (MQ=255)
                                            aGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAg                                                                                                      <  2:1789456/150‑1 (MQ=255)
                                            aGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAg                                                                                                      >  2:1321274/1‑150 (MQ=255)
                                              cGAACGGACACATCAGGTCGATAACATTGATACCGGTATCCAGCAGTTCCTGAGAGTTTGACAGCTCTTAGTAGGAAGGTGCAGCGCGCTGAATCGCACAAAGCTCATCTTCACCGATCACGCCTTTCATGTCTACCGGGTCACCCAGTa                                                                                                    >  2:1175399/1‑150 (MQ=255)
                                               gAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTAc                                                                                                   >  1:2935369/1‑150 (MQ=255)
                                               gAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTAc                                                                                                   <  1:4256282/150‑1 (MQ=255)
                                               gAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTAc                                                                                                   <  1:162181/150‑1 (MQ=255)
                                               gAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTAc                                                                                                   >  1:2965488/1‑150 (MQ=255)
                                                aaCGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACg                                                                                                  <  1:4191139/150‑1 (MQ=255)
                                                  tggACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGtt                                                                                                >  1:1687138/2‑150 (MQ=255)
                                                                  aTAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCaga                                                                                <  2:3195995/150‑1 (MQ=255)
                                                                   tAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCagag                                                                               <  1:4195020/150‑1 (MQ=255)
                                                                     aCTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTACAAGACTACCGGTTCACCCAGTACGATCATGATACGGCCCAGAGTc                                                                             >  2:3415323/1‑150 (MQ=255)
                                                                       tttGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATATCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGc                                                                           <  2:4193873/150‑1 (MQ=255)
                                                                         tGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCtt                                                                         <  1:1328423/150‑1 (MQ=255)
                                                                          taTACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCttt                                                                        >  2:4381571/2‑150 (MQ=255)
                                                                          gATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCttt                                                                        >  1:3038814/1‑150 (MQ=255)
                                                                           aTACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTta                                                                       <  2:1564331/150‑1 (MQ=255)
                                                                            tACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtac                                                                      <  1:2777505/150‑1 (MQ=255)
                                                                               cGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtaccta                                                                   <  2:2382357/150‑1 (MQ=255)
                                                                                ggTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCACGATACGGCCCAGAGTCGCTTtacctac                                                                  >  2:3138472/1‑150 (MQ=255)
                                                                                   ttCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtacctacccg                                                               >  2:1897168/1‑148 (MQ=255)
                                                                                   ttCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCgg                                                               <  1:2840461/150‑1 (MQ=255)
                                                                                      cagcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGAc                                                            <  1:505963/150‑1 (MQ=255)
                                                                                       agcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCTACCGGGACt                                                           >  2:13930/1‑150 (MQ=255)
                                                                                        gcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATAGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACtt                                                          <  1:2065032/150‑1 (MQ=255)
                                                                                          agTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCa                                                        >  2:4703890/1‑150 (MQ=255)
                                                                                          agTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTAACTACCGGGACTTCa                                                        >  2:454436/1‑150 (MQ=255)
                                                                                            ttCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACGTCAAt                                                      >  2:554957/1‑150 (MQ=255)
                                                                                                tGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCggg                                                  <  2:2202221/150‑1 (MQ=255)
                                                                                                tGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCggg                                                  >  1:1288029/1‑150 (MQ=255)
                                                                                                 gagaGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCTACCCGGACGTAAATAGGgt                                                 >  1:3626928/1‑150 (MQ=255)
                                                                                                  agcggttGACAGCTCTGCGTAGGAAGGTGCTGCGCGGTGACTCGCCCACCCCACGACTTCCCCGAGCTCGCCGTCCATCTCTACCGGTTCACCCAGTCCGTTCATGATACGGCCCAGAGTCGCTTTCCCTACAGGGACTTCAATCGGgtg                                                <  2:4829628/145‑1 (MQ=255)
                                                                                                    agTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACATCAATCGGGTGtt                                              >  2:4058296/1‑150 (MQ=255)
                                                                                                     gTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTc                                             <  1:3961458/150‑1 (MQ=255)
                                                                                                       ttGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGa                                           >  2:3761913/1‑150 (MQ=255)
                                                                                                        tGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAg                                          >  2:471189/1‑150 (MQ=255)
                                                                                                        tGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAg                                          <  2:2981458/150‑1 (MQ=255)
                                                                                                          aCAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGt                                        <  2:4330649/150‑1 (MQ=255)
                                                                                                           cAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTc                                       <  2:5022149/150‑1 (MQ=255)
                                                                                                             gCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACAAACCGGGACATCAATCGGGTGGTCGACGTCtt                                     >  1:3074060/1‑150 (MQ=255)
                                                                                                              ctctTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGGCATCAATCGGGTGTTCGAGGTCttt                                    >  1:1638955/1‑150 (MQ=255)
                                                                                                                   cGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACAt                               <  1:4050422/150‑1 (MQ=255)
                                                                                                                   cGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACAt                               <  1:1472557/150‑1 (MQ=255)
                                                                                                                    gTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTGTACCTACCGGGACTTCAATAGGGTGTTCGAGGTCTTTTACATc                              >  2:3542438/1‑150 (MQ=255)
                                                                                                                     tAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACAtac                             >  2:263604/1‑148 (MQ=255)
                                                                                                                     tAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATcc                             >  2:2161365/1‑150 (MQ=255)
                                                                                                                     tAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTATTTTACATcc                             >  2:1946636/1‑150 (MQ=255)
                                                                                                                     tAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTTCTGCCGGGGCTTGAATAGGGTGGTGGAGGTGTTTTACAtac                             >  1:883128/1‑148 (MQ=255)
                                                                                                                     tAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGACGCTTTACCTAACAGGACTTCAAACGGGTGTTCGAGGTCTTTTACATcc                             >  2:855008/1‑150 (MQ=255)
                                                                                                                      aGGAAGGTGCTGCGCGGTGAATCGCCCAATGCTCTTCTTCACCGATCTCTCCTTTCATGTCTACAGGTTCAACCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCAACAGGGACTTAAATAGGGTGATCGAGGTGTTATActtgca                            >  2:5073801/1‑145 (MQ=255)
                                                                                                                      aGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCa                            <  2:661799/150‑1 (MQ=255)
                                                                                                                      aGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCa                            <  1:791976/150‑1 (MQ=255)
                                                                                                                      aGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCa                            >  1:371823/1‑150 (MQ=255)
                                                                                                                       ggAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAg                           <  2:1048393/150‑1 (MQ=255)
                                                                                                                        gAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGa                          >  1:2349469/1‑150 (MQ=255)
                                                                                                                            gTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTAAATCCAGACcgc                      >  1:1987425/1‑150 (MQ=255)
                                                                                                                            gTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGGACGGTCATGATACGGCCCAGAGTCGCTTTACCGACCGGGACTTCAATCGGGGTTTCGAGGTCTTTTACATACAGACcgc                      >  2:2631234/1‑150 (MQ=255)
                                                                                                                             tgctgcGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACcgcg                     <  1:4729442/150‑1 (MQ=255)
                                                                                                                               ctgcGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGAc                   <  1:2721885/150‑1 (MQ=255)
                                                                                                                                tgcGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTGTTTTACATCCAGACCGCGACg                  >  1:5142322/1‑150 (MQ=255)
                                                                                                                                tgcGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACg                  <  1:453391/150‑1 (MQ=255)
                                                                                                                                tgcGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTCCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTTCATCCAGACCGCGACg                  >  1:2386187/1‑150 (MQ=255)
                                                                                                                                 gcgcGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTACTACCGGGACTTCAATCGGGTGTTAGAGGTCTTTTTCATACAGACCGCGACGc                 >  1:1176306/1‑150 (MQ=255)
                                                                                                                                      gTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGAcc            <  2:1020502/150‑1 (MQ=255)
                                                                                                                                        gAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGt          <  1:1201045/150‑1 (MQ=255)
                                                                                                                                        gAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTAACTAACGGGACGTCAATAGGGTGTTCGAGGTCTTTTAAAAACAGACCCCCACGCACAccct          >  1:4855978/1‑148 (MQ=255)
                                                                                                                                           tCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCgg       <  2:3677564/150‑1 (MQ=255)
                                                                                                                                            cGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCgga      <  2:2313721/150‑1 (MQ=255)
                                                                                                                                              cccAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTCCAGGGGAATAAATCGGGTGGTCGCGGTCTTTTACATCCAGACCGCGACGCAGCCCGTAggtgg    >  1:2701733/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAA  >  CP009273/3910269‑3910562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑