Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,910,448 G→T 100% V96V (GTC→GTA)  atpD ← F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,910,4480GT98.8% 904.6 / ‑0.3 281V96V (GTC→GTA) atpDF1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (4/0);  new base T (147/129);  total (152/129)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 281 total aligned reads displayed.

TTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGC  >  CP009273/3910301‑3910593
                                                                                                                                                   |                                                                                                                                                 
tttACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTAc                                                                                                                                                 <  2:1793875/150‑1 (MQ=255)
tttACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTAc                                                                                                                                                 <  1:1823704/150‑1 (MQ=255)
tttACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTAc                                                                                                                                                 <  2:1631203/150‑1 (MQ=255)
 ttACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTAcc                                                                                                                                                <  2:2121598/150‑1 (MQ=255)
  tACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCg                                                                                                                                               >  2:2749586/1‑150 (MQ=255)
     cGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGtt                                                                                                                                            <  1:3746745/150‑1 (MQ=255)
           tAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCa                                                                                                                                      >  1:661799/1‑150 (MQ=255)
              cGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTa                                                                                                                                   >  2:1397160/1‑150 (MQ=255)
              cGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTa                                                                                                                                   >  1:834706/1‑150 (MQ=255)
              cGAACGGACACATCAGGTCGATAACATTGATACCGGTATCCAGCAGTTCCTGAGAGTTTGACAGCTCTTAGTAGGAAGGTGCAGCGCGCTGAATCGCACAAAGCTCATCTTCACCGATCACGCCTTTCATGTCTACCGGGTCACCCAGTa                                                                                                                                   >  2:1175399/1‑150 (MQ=255)
               gAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTAc                                                                                                                                  <  1:162181/150‑1 (MQ=255)
                aaCGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACg                                                                                                                                 >  2:3732468/1‑150 (MQ=255)
                aaCGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACg                                                                                                                                 >  2:2261669/1‑150 (MQ=255)
                     acacATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCAt                                                                                                                            >  1:4870839/1‑150 (MQ=255)
                        caTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGAt                                                                                                                         >  1:5146469/1‑150 (MQ=255)
                          tCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATAc                                                                                                                       <  2:1320217/150‑1 (MQ=255)
                              gTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGcc                                                                                                                   <  1:1684388/150‑1 (MQ=255)
                              gTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGcc                                                                                                                   >  1:4046684/1‑150 (MQ=255)
                                 gATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCag                                                                                                                >  2:3126987/1‑150 (MQ=255)
                                   tAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCagag                                                                                                              <  1:4195020/150‑1 (MQ=255)
                                     aCTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTc                                                                                                            <  1:980324/150‑1 (MQ=255)
                                     aCTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTACAAGACTACCGGTTCACCCAGTACGATCATGATACGGCCCAGAGTc                                                                                                            >  2:3415323/1‑150 (MQ=255)
                                     aCTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTAGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTc                                                                                                            >  2:831199/1‑150 (MQ=255)
                                           aTACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTta                                                                                                      >  2:1514484/1‑150 (MQ=255)
                                           aTACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTta                                                                                                      >  1:3000359/1‑150 (MQ=255)
                                           aTACCGGTGTCCAGCATTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGAGGTGAATCGCCCAAAGCTCTTCTTAACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGAACGTTCATGATACGGCCCAGAGTCGCTTta                                                                                                      >  2:5071984/1‑150 (MQ=255)
                                            tACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtac                                                                                                     <  1:2777505/150‑1 (MQ=255)
                                               cGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtaccta                                                                                                  <  2:2382357/150‑1 (MQ=255)
                                                 gTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtacctacc                                                                                                >  2:136222/1‑150 (MQ=255)
                                                   ttCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtacctacccg                                                                                              >  2:1897168/1‑148 (MQ=255)
                                                    tCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCggg                                                                                             >  1:1637081/1‑150 (MQ=255)
                                                    tCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCggg                                                                                             >  2:4802083/1‑150 (MQ=255)
                                                    tCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCggg                                                                                             <  1:1397160/150‑1 (MQ=255)
                                                    tCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTAACTAACggg                                                                                             >  1:2254076/1‑150 (MQ=255)
                                                      cagcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGAc                                                                                           >  1:705111/1‑150 (MQ=255)
                                                      cagcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGAc                                                                                           <  1:505963/150‑1 (MQ=255)
                                                       agcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACt                                                                                          <  2:2635603/150‑1 (MQ=255)
                                                        gcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATAGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACtt                                                                                         <  1:2065032/150‑1 (MQ=255)
                                                         cagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTc                                                                                        <  1:4722955/150‑1 (MQ=255)
                                                          agTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCa                                                                                       <  1:1321274/150‑1 (MQ=255)
                                                          agTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCa                                                                                       >  2:4703890/1‑150 (MQ=255)
                                                          agTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCa                                                                                       >  2:453391/1‑150 (MQ=255)
                                                            ttCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCAACCGGGACTTCAAt                                                                                     >  2:559583/1‑150 (MQ=255)
                                                                 gagaGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCTACCCGGACGTAAATAGGgt                                                                                >  1:3626928/1‑150 (MQ=255)
                                                                    agTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGtt                                                                             >  2:365003/1‑150 (MQ=255)
                                                                     gTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTc                                                                            <  1:2011208/150‑1 (MQ=255)
                                                                     gTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGGGTTc                                                                            >  1:3233665/1‑150 (MQ=255)
                                                                        tGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAg                                                                         >  2:471189/1‑150 (MQ=255)
                                                                        tGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAg                                                                         <  2:4150727/150‑1 (MQ=255)
                                                                          aCAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGt                                                                       <  2:4330649/150‑1 (MQ=255)
                                                                          aCAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCAACCGGGACATCAATCGGGTGTTCGAGGt                                                                       >  1:2946153/1‑150 (MQ=255)
                                                                            aGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCt                                                                     <  2:33279/150‑1 (MQ=255)
                                                                             gCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTAGAGGTCtt                                                                    >  1:2851041/1‑150 (MQ=255)
                                                                             gCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCTACCGGGACTTCAATAGGGTGTTAGAGGTCtt                                                                    >  1:3073816/1‑150 (MQ=255)
                                                                              ctctTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCttt                                                                   <  2:4008910/150‑1 (MQ=255)
                                                                                  tCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACa                                                               <  2:5146469/150‑1 (MQ=255)
                                                                                   cGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACAt                                                              <  1:1472557/150‑1 (MQ=255)
                                                                                    gTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTGTACCTACCGGGACTTCAATAGGGTGTTCGAGGTCTTTTACATc                                                             >  2:3542438/1‑150 (MQ=255)
                                                                                     tAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATcc                                                            >  2:263661/1‑150 (MQ=255)
                                                                                     tAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATcc                                                            >  2:2161365/1‑150 (MQ=255)
                                                                                     tAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATcc                                                            <  2:4369547/150‑1 (MQ=255)
                                                                                      aGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCa                                                           <  2:661799/150‑1 (MQ=255)
                                                                                        gAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGa                                                         >  1:2349469/1‑150 (MQ=255)
                                                                                           ggTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACcg                                                      <  1:4515192/150‑1 (MQ=255)
                                                                                             tgctgcGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACcgcg                                                    >  2:3163283/1‑150 (MQ=255)
                                                                                               ctgcGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACGTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGAc                                                  >  2:382275/1‑150 (MQ=255)
                                                                                                tgcGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTGTTTTACATCCAGACCGCGACg                                                 >  1:5142322/1‑150 (MQ=255)
                                                                                                tgcGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTCCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTTCATCCAGACCGCGACg                                                 >  1:2386187/1‑150 (MQ=255)
                                                                                                 gcgcGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTACTACCGGGACTTCAATCGGGTGTTAGAGGTCTTTTTCATACAGACCGCGACGc                                                >  1:1176306/1‑150 (MQ=255)
                                                                                                     ggTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTAACTACCGGGACTTAAATCGGGTGTTCGAGGTCTTTTAAATACAGACCGCGACGCAGAc                                            >  2:34851/1‑150 (MQ=255)
                                                                                                      gTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGAcc                                           <  2:1020502/150‑1 (MQ=255)
                                                                                                           tCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTCCAACCCGGACTTCAATCGGGTGTTAGAGGTCTTTTAAATACAAACCGCCACGCAGACCCTCgg                                      >  1:3106201/1‑150 (MQ=255)
                                                                                                            cGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCgga                                     <  2:2313742/150‑1 (MQ=255)
                                                                                                            cGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTACTACCGGGACTTAAAACGGGTGTTAGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCcga                                     >  1:4421768/1‑150 (MQ=255)
                                                                                                              cccAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTCCAGGGGAATAAATCGGGTGGTCGCGGTCTTTTACATCCAGACCGCGACGCAGCCCGTAggtgg                                   >  1:2701733/1‑150 (MQ=255)
                                                                                                              cccAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTTCATCCAGACCCCGACGCCGACCGTCggagg                                   >  1:4662484/1‑150 (MQ=255)
                                                                                                                cAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACAACCAGACCGCCACGCAGACCCGCGGAGGaa                                 >  1:4259028/1‑150 (MQ=255)
                                                                                                                  aagCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAAcc                               <  1:2913639/148‑1 (MQ=255)
                                                                                                                  aagCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCAACCCGGACATCAATCGGGTGTTCGAGGTCTTTAACAACCACACCGCGACCCAGACCCACggaggcccc                               >  2:3028070/3‑146 (MQ=255)
                                                                                                                   agCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTCCTACCGGGACGTCAATCGGGTGTTCGAGGTCTTTTTCATCCACACCGCGACGCAGACCGTCGGAGGCAccc                              >  2:4419069/2‑150 (MQ=255)
                                                                                                                        ttcttcACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGc                         <  2:4980462/150‑1 (MQ=255)
                                                                                                                            tcACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATg                     <  2:872051/150‑1 (MQ=255)
                                                                                                                            tcACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATg                     <  2:2900244/150‑1 (MQ=255)
                                                                                                                             cACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTCCTACCCGGACTTCAATCGGGTGTTCGAGGTGTTTTTAAACCAGACCGCGACGCAGACCGTCGGGGGAACCCCTTGCGATgg                    >  1:4130834/1‑150 (MQ=255)
                                                                                                                                cGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCCGGACTTCAAAAGGGTGTTAGAGGTCGTTTTCATACAGACCGCCACGCAGACCCTCGGAGGAAACCAATGCGCTTgtac                 >  1:3900211/1‑150 (MQ=255)
                                                                                                                                cGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCTAACCGGACTTCAAAAGGGTGTTCGAGGTCTTTTAAAAACAGACCCCGACGCAGACCCTCGGAGGAACCCAATGCGAAGgtac                 >  1:2469116/1‑150 (MQ=255)
                                                                                                                                 gATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGgtacg                <  1:4799542/150‑1 (MQ=255)
                                                                                                                                    ctcGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGgtacgtac             <  2:739364/150‑1 (MQ=255)
                                                                                                                                    ctcGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGgtacgtac             <  2:3524005/150‑1 (MQ=255)
                                                                                                                                     tcGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGtacgtacg            <  1:1060984/150‑1 (MQ=255)
                                                                                                                                       gCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGAt          <  1:2749586/150‑1 (MQ=255)
                                                                                                                                        ccTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTAGAGGTCTTTTACATACAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATa         >  2:4890193/1‑150 (MQ=255)
                                                                                                                                          tttCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATAcc       <  2:1779690/150‑1 (MQ=255)
                                                                                                                                          tttCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCAAACCGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCCGACCCCGACGCAGACCCTCGGAGGAACCCAATGCGATGGTACGGACGATAcc       >  1:3684482/1‑150 (MQ=255)
                                                                                                                                          tttCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTACTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTTCATCCAGACCGCGACGCAGACCGTCGGAGGAACCCAATGCGATGGTACGTACGATAcc       >  1:1740334/1‑150 (MQ=255)
                                                                                                                                          tttCATGTCAACCGTTTCACCCAGTACGTTCATGATACGGACCAGATTCGCTTTAAAAAACCGGAATTCAATCGGGTGTTAGATGTCTTTTACATAAACACCGCGACGCAGACCGACGGATGAACCCATTGCGATGGTACGTACGATaac       >  2:1229204/1‑148 (MQ=255)
                                                                                                                                           ttCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATAccg      <  1:4703890/150‑1 (MQ=255)
                                                                                                                                           ttCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATAccg      >  2:2587141/1‑150 (MQ=255)
                                                                                                                                              aTGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGGTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATAccgccg   >  2:4212872/1‑150 (MQ=255)
                                                                                                                                               tGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATAccgccgc  >  2:1922711/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGC  >  CP009273/3910301‑3910593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑