Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,917,554 C→A 100% L480L (CTG→CTT)  mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,917,5540CA98.2% 889.0 / 3.9 276L480L (CTG→CTT) mnmG5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base A (144/126);  total (147/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 276 total aligned reads displayed.

AAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTGCGCG  >  CP009273/3917407‑3917700
                                                                                                                                                   |                                                                                                                                                  
  aCGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgac                                                                                                                                                <  1:77086/150‑1 (MQ=255)
   cGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacg                                                                                                                                               <  2:1528846/150‑1 (MQ=255)
   cGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacg                                                                                                                                               >  1:4126622/1‑150 (MQ=255)
   cGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacg                                                                                                                                               >  2:4145493/1‑150 (MQ=255)
     gCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgct                                                                                                                                             >  1:564665/1‑150 (MQ=255)
     gCGTCAGCGTGGTTAATTTTTCATAAGTCATGTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgct                                                                                                                                             <  1:2094404/150‑1 (MQ=255)
      cGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctg                                                                                                                                            <  2:426577/150‑1 (MQ=255)
       gTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctga                                                                                                                                           >  1:2954016/1‑150 (MQ=255)
       gTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctga                                                                                                                                           >  1:4148422/1‑150 (MQ=255)
         cAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAgacgctgacg                                                                                                                                         >  1:3406057/1‑150 (MQ=255)
            cGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctc                                                                                                                                      <  1:2050778/150‑1 (MQ=255)
            cGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctc                                                                                                                                      <  1:4621638/150‑1 (MQ=255)
             gTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctca                                                                                                                                     >  2:4040203/1‑150 (MQ=255)
              tGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGacgctcac                                                                                                                                    <  2:1202144/150‑1 (MQ=255)
                   aaTTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCg                                                                                                                               <  2:5126341/150‑1 (MQ=255)
                    aTTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGa                                                                                                                              >  2:3924673/1‑150 (MQ=255)
                      ttttCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGata                                                                                                                            <  1:2223647/150‑1 (MQ=255)
                      ttttCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGata                                                                                                                            <  2:5047481/150‑1 (MQ=255)
                       tttCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGatat                                                                                                                           <  2:2764049/150‑1 (MQ=255)
                       tttCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGatat                                                                                                                           <  1:1111995/150‑1 (MQ=255)
                         tCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATtc                                                                                                                         <  2:83367/150‑1 (MQ=255)
                            tAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCa                                                                                                                      >  2:1130129/1‑150 (MQ=255)
                             aaGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCaa                                                                                                                     >  1:454522/1‑150 (MQ=255)
                              aGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAg                                                                                                                    >  2:2244290/1‑150 (MQ=255)
                                  aTTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTtc                                                                                                                <  1:3322260/150‑1 (MQ=255)
                                  aTTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTtc                                                                                                                >  2:2466540/1‑150 (MQ=255)
                                  aTTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTtc                                                                                                                >  1:3924531/1‑150 (MQ=255)
                                    ttCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTtctc                                                                                                              >  1:2715874/1‑150 (MQ=255)
                                       cGGACGACGCAGCAGATCTGCTCCACGGGCTTCACGTGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGtt                                                                                                           <  2:2946872/150‑1 (MQ=255)
                                        ggACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTa                                                                                                          <  2:1319171/150‑1 (MQ=255)
                                        ggACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTa                                                                                                          >  2:3743373/1‑150 (MQ=255)
                                           cgacgCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAg                                                                                                       <  2:2303506/150‑1 (MQ=255)
                                              cgCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAgcgc                                                                                                    >  1:2870859/1‑150 (MQ=255)
                                                cagcagATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAgcgcgc                                                                                                  >  1:4019078/1‑150 (MQ=255)
                                                 agcagATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGcc                                                                                                 >  2:1932454/1‑150 (MQ=255)
                                                  gcagATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGccc                                                                                                >  1:1788195/1‑150 (MQ=255)
                                                    agATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCaa                                                                                              >  2:345288/1‑150 (MQ=255)
                                                      aTCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACg                                                                                            <  1:1093324/150‑1 (MQ=255)
                                                        cTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGtt                                                                                          <  2:949468/150‑1 (MQ=255)
                                                          tCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCg                                                                                        <  2:2096092/150‑1 (MQ=255)
                                                           caccacTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGt                                                                                       >  1:1727796/1‑150 (MQ=255)
                                                           caccacTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGt                                                                                       >  2:2242042/1‑150 (MQ=255)
                                                            accacTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTc                                                                                      >  2:1090129/1‑150 (MQ=255)
                                                            accacTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTACCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTc                                                                                      >  1:5134849/1‑150 (MQ=255)
                                                             ccacTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTcg                                                                                     <  1:3964183/150‑2 (MQ=255)
                                                              cacTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCAt                                                                                    >  1:2548992/1‑150 (MQ=255)
                                                                cTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATcc                                                                                  >  2:2207291/1‑150 (MQ=255)
                                                                cTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATcc                                                                                  >  2:2591718/1‑150 (MQ=255)
                                                                cTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATcc                                                                                  >  2:1056940/1‑150 (MQ=255)
                                                                 tGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATcca                                                                                 >  2:1904815/1‑150 (MQ=255)
                                                                      tCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTCAAGTGCGCCCAACGTTCGTCATCCACCAgg                                                                            >  2:3711509/1‑150 (MQ=255)
                                                                      tCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGTGCGCCCAACGTTCGTCATCCACCAgg                                                                            >  2:3164382/1‑150 (MQ=255)
                                                                          gggAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCa                                                                        <  2:2381997/150‑1 (MQ=255)
                                                                          gggAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCa                                                                        <  2:3608404/150‑1 (MQ=255)
                                                                            gAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGt                                                                      >  2:2481965/1‑150 (MQ=255)
                                                                            gAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGt                                                                      <  1:2521010/150‑1 (MQ=255)
                                                                             aaaGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGtt                                                                     <  2:1664080/150‑1 (MQ=255)
                                                                              aaGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTc                                                                    >  1:1528129/1‑150 (MQ=255)
                                                                                gcggcgCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCAc                                                                  <  1:1997070/150‑1 (MQ=255)
                                                                                    cgcAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGAcc                                                              <  1:3847328/150‑1 (MQ=255)
                                                                                    cgcAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGAcc                                                              <  1:3537867/150‑1 (MQ=255)
                                                                                       aGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGAt                                                           >  2:4617359/1‑150 (MQ=255)
                                                                                           aGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCa                                                       <  1:3591643/150‑1 (MQ=255)
                                                                                           aGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCa                                                       >  1:3497995/1‑150 (MQ=255)
                                                                                           aGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCa                                                       >  2:2783098/1‑150 (MQ=255)
                                                                                            ggTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAg                                                      <  1:3711384/150‑1 (MQ=255)
                                                                                             gTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGt                                                     >  2:1867716/1‑150 (MQ=255)
                                                                                                   aTTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACg                                               <  2:157087/150‑1 (MQ=255)
                                                                                                    ttCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGc                                              <  2:1011094/150‑1 (MQ=255)
                                                                                                    ttCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGc                                              >  1:50402/1‑150 (MQ=255)
                                                                                                      cACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAg                                            <  1:2126558/150‑1 (MQ=255)
                                                                                                       aCTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGa                                           >  1:1132519/1‑150 (MQ=255)
                                                                                                       aCTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGa                                           >  1:4802523/1‑150 (MQ=255)
                                                                                                       aCTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGa                                           >  1:4405511/1‑150 (MQ=255)
                                                                                                       aCTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGa                                           >  2:47858/1‑150 (MQ=255)
                                                                                                       aCTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGa                                           <  2:4256410/150‑1 (MQ=255)
                                                                                                        cTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGAt                                          <  2:4198234/150‑1 (MQ=255)
                                                                                                         ttCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATc                                         >  2:3574386/1‑150 (MQ=255)
                                                                                                         ttCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTGTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATc                                         >  2:4434582/1‑150 (MQ=255)
                                                                                                          tCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATcc                                        >  1:1343517/1‑150 (MQ=255)
                                                                                                           cGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCg                                       <  2:4518105/150‑1 (MQ=255)
                                                                                                               tGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCAtt                                   <  1:3106806/150‑1 (MQ=255)
                                                                                                               tGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCAtt                                   >  2:287522/1‑150 (MQ=255)
                                                                                                                  aGCTTCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATc                                >  2:3885413/1‑150 (MQ=255)
                                                                                                                     ttCCGCCGACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTc                             <  2:3087966/150‑1 (MQ=255)
                                                                                                                      tCCGCCGACGGGGTTACCCTGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAAAGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTcg                            <  2:11583/150‑1 (MQ=255)
                                                                                                                        cgccgACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGACATCCACCCGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTcgcg                          >  1:1286678/1‑150 (MQ=255)
                                                                                                                           cgACGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAg                       >  2:4952949/1‑150 (MQ=255)
                                                                                                                             aCGGGGTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCa                     <  1:3773618/150‑1 (MQ=255)
                                                                                                                               ggggTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATc                   >  1:1871172/1‑150 (MQ=255)
                                                                                                                               ggggTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATc                   >  2:4706965/1‑150 (MQ=255)
                                                                                                                                gggTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCa                  >  1:4212578/1‑150 (MQ=255)
                                                                                                                                gggTTACCCAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCa                  <  1:4284186/150‑1 (MQ=255)
                                                                                                                                      cccAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGat            <  2:3058696/150‑1 (MQ=255)
                                                                                                                                      cccAGGTCGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGat            >  2:377400/1‑150 (MQ=255)
                                                                                                                                             cGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTgc     <  1:2244395/150‑1 (MQ=255)
                                                                                                                                             cGATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTgc     >  2:1457595/1‑150 (MQ=255)
                                                                                                                                             cGATTTAAGACGCTGACGCACACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTgc     >  1:900808/1‑150 (MQ=255)
                                                                                                                                              gATTTAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTgcg    >  2:766543/1‑150 (MQ=255)
                                                                                                                                                tttAAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTgcgcg  <  1:1430501/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGACCGATTTCAGTCAAACGCAGATCCGCATTATCTTCGCGTAGCATCAGACGATATTCTGCGCG  >  CP009273/3917407‑3917700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑