Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 329,372 A→T 100% L258Q (CTG→CAG)  yahB ← putative DNA‑bindng transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273329,3720AT98.5% 1068.9 / 2.1 321L258Q (CTG→CAG) yahBputative DNA‑bindng transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base T (157/159);  total (159/162)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 326 total aligned reads displayed.

TGCAGTAAATCCTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTATT  >  CP009273/329225‑329517
                                                                                                                                                   |                                                                                                                                                 
tGCAGTAAATCCTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACctgc                                                                                                                                                 <  1:1152507/150‑1 (MQ=255)
    gTAAATCCTGATAGATCCCCGTTAATATGCCATTTGTCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAggg                                                                                                                                             <  1:464602/150‑1 (MQ=255)
    gTAAATCCTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAggg                                                                                                                                             >  1:4774699/1‑150 (MQ=255)
    gTAAATCCTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAggg                                                                                                                                             >  2:232267/1‑150 (MQ=255)
       aaTCCTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGAtt                                                                                                                                          <  2:4964165/150‑1 (MQ=255)
       aaTCCTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGAtt                                                                                                                                          <  2:4498370/150‑1 (MQ=255)
          ccTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCg                                                                                                                                       <  1:1974090/150‑1 (MQ=255)
            tGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTc                                                                                                                                     <  2:1665929/150‑1 (MQ=255)
            tGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTc                                                                                                                                     >  1:2844959/1‑150 (MQ=255)
             gATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGAAGGGATTGCGTCa                                                                                                                                    >  1:3023877/1‑150 (MQ=255)
              aTAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCAt                                                                                                                                   >  2:2567557/1‑150 (MQ=255)
                aGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCg                                                                                                                                 <  2:406957/150‑1 (MQ=255)
                aGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCg                                                                                                                                 >  2:1433424/1‑150 (MQ=255)
                  aTCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGcc                                                                                                                               >  1:4334428/1‑150 (MQ=255)
                   tCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGcca                                                                                                                              <  1:3168288/150‑2 (MQ=255)
                       cGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACg                                                                                                                          >  2:296205/1‑150 (MQ=255)
                       cGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACg                                                                                                                          <  2:2128647/150‑1 (MQ=255)
                        gTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACgg                                                                                                                         >  2:4147170/1‑150 (MQ=255)
                        gTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACgg                                                                                                                         >  1:3531292/1‑150 (MQ=255)
                        gTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACgg                                                                                                                         >  2:1066939/1‑150 (MQ=255)
                          tAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGAc                                                                                                                       >  2:3627007/1‑150 (MQ=255)
                           aaTATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGGTTGCGTCATCGCCTCACGGAcc                                                                                                                      >  1:340572/1‑150 (MQ=255)
                           aaTATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGAcc                                                                                                                      >  2:3428163/1‑150 (MQ=255)
                              atGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATg                                                                                                                   >  1:2552969/1‑150 (MQ=255)
                              atGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATg                                                                                                                   >  1:3104610/1‑150 (MQ=255)
                              atGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATg                                                                                                                   >  1:2316115/1‑150 (MQ=255)
                               tGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGAGTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGt                                                                                                                  <  2:4742020/150‑1 (MQ=255)
                                 ccATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTaa                                                                                                                <  1:2355305/150‑1 (MQ=255)
                                  cATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAAt                                                                                                               >  1:640478/1‑150 (MQ=255)
                                    tttGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATcc                                                                                                             <  1:141640/150‑1 (MQ=255)
                                      tGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCgg                                                                                                           <  1:520277/150‑1 (MQ=255)
                                      tGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCgg                                                                                                           <  1:349973/150‑1 (MQ=255)
                                       ggCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGc                                                                                                          >  1:221769/1‑150 (MQ=255)
                                           cAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTACCCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCaaaa                                                                                                      >  1:3138433/1‑150 (MQ=255)
                                           cAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCaaaa                                                                                                      <  2:5017803/150‑1 (MQ=255)
                                            aaaTTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCaaaaa                                                                                                     <  2:3394439/150‑1 (MQ=255)
                                               ttGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAAcc                                                                                                  <  1:4425105/150‑1 (MQ=255)
                                                  ttttttAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAAt                                                                                               >  1:1034454/1‑150 (MQ=255)
                                                  ttctgtcaTCCACGGCGTCACCTGCCCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAAt                                                                                               <  2:2939691/143‑1 (MQ=255)
                                                     tttAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTcc                                                                                            <  2:1475519/150‑1 (MQ=255)
                                                     tttAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTcc                                                                                            <  2:4549846/150‑1 (MQ=255)
                                                       tAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCtt                                                                                          <  2:546858/150‑1 (MQ=255)
                                                       tAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCtt                                                                                          <  2:3916620/150‑1 (MQ=255)
                                                       tAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCtt                                                                                          >  1:4692364/1‑150 (MQ=255)
                                                        aaTCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTc                                                                                         >  2:1225627/1‑150 (MQ=255)
                                                         ctCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCa                                                                                        <  2:1867101/149‑1 (MQ=255)
                                                           ccACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCAcc                                                                                      >  1:3877763/1‑150 (MQ=255)
                                                           ccACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCAcc                                                                                      >  1:3703768/1‑150 (MQ=255)
                                                            cACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCt                                                                                     >  2:778376/1‑150 (MQ=255)
                                                             aCTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTa                                                                                    >  1:3303891/1‑150 (MQ=255)
                                                               tGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTaaa                                                                                  >  2:1926510/1‑150 (MQ=255)
                                                               tGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTaaa                                                                                  <  1:4253795/150‑1 (MQ=255)
                                                                gCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTaaaa                                                                                 <  1:1344576/150‑1 (MQ=255)
                                                                gCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTaaaa                                                                                 <  2:1794253/150‑1 (MQ=255)
                                                                gCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTaaaa                                                                                 >  1:820070/1‑150 (MQ=255)
                                                                   tCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCt                                                                              <  2:3546144/150‑1 (MQ=255)
                                                                      ccTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGc                                                                           <  2:2756316/150‑1 (MQ=255)
                                                                       cTGACCCGTAGCAGAATGCTGAGTCGCCAGTTACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCAACTAAAATCTGGCa                                                                          <  2:151992/150‑1 (MQ=255)
                                                                        tGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGTCAc                                                                         >  1:4968256/1‑150 (MQ=255)
                                                                           cccGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGa                                                                      <  2:3735694/150‑1 (MQ=255)
                                                                              gTAGCAGAATGCTGAGTCGCCAGTACCAGGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACAt                                                                   <  2:2630636/150‑1 (MQ=255)
                                                                               tAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACAtt                                                                  >  1:2638521/1‑150 (MQ=255)
                                                                               tAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACAtt                                                                  >  1:2238455/1‑150 (MQ=255)
                                                                                 gCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTg                                                                <  2:1381188/150‑1 (MQ=255)
                                                                                 gCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTg                                                                >  1:4554908/1‑150 (MQ=255)
                                                                                      aTGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGtt                                                           >  2:3111874/1‑150 (MQ=255)
                                                                                            aGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATc                                                     >  1:2051427/1‑150 (MQ=255)
                                                                                                gCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGc                                                 >  2:1040079/1‑150 (MQ=255)
                                                                                                  cAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCAc                                               >  1:5057156/1‑150 (MQ=255)
                                                                                                   aGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCAcc                                              >  2:4783171/1‑150 (MQ=255)
                                                                                                   aGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCAcc                                              >  1:734180/1‑150 (MQ=255)
                                                                                                     tAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAg                                            >  2:2167415/1‑150 (MQ=255)
                                                                                                     tAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAg                                            <  1:296205/150‑1 (MQ=255)
                                                                                                     tAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAg                                            >  2:2068784/1‑150 (MQ=255)
                                                                                                         aTGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAAtt                                        <  1:4919286/150‑1 (MQ=255)
                                                                                                             gcgAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACt                                    <  2:2029189/150‑1 (MQ=255)
                                                                                                              cgAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTc                                   >  2:3055439/1‑150 (MQ=255)
                                                                                                                 aTCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTg                                <  1:2019648/150‑1 (MQ=255)
                                                                                                                    cTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGccc                             <  2:4169379/150‑1 (MQ=255)
                                                                                                                    cTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGccc                             <  2:3662774/150‑1 (MQ=255)
                                                                                                                    cTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGccc                             <  2:3901445/150‑1 (MQ=255)
                                                                                                                     tGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCg                            <  1:2499310/150‑1 (MQ=255)
                                                                                                                      ggCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGt                           <  1:778376/150‑1 (MQ=255)
                                                                                                                        cgcgGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTgc                         <  1:4027604/150‑1 (MQ=255)
                                                                                                                         gcgGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTgca                        >  1:2727995/1‑150 (MQ=255)
                                                                                                                          cgGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTgcag                       >  1:1321581/1‑150 (MQ=255)
                                                                                                                          cgGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTgcag                       >  2:621618/1‑150 (MQ=255)
                                                                                                                            gATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTgcagca                     <  1:2695815/150‑1 (MQ=255)
                                                                                                                                   gATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGcc              <  1:169434/150‑1 (MQ=255)
                                                                                                                                   gATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGcc              <  1:160144/150‑1 (MQ=255)
                                                                                                                                   gATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGC‑AAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGccc             >  1:3783242/1‑150 (MQ=255)
                                                                                                                                       tGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACt          >  1:886829/1‑150 (MQ=255)
                                                                                                                                          cGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACtttt       >  1:1892786/1‑150 (MQ=255)
                                                                                                                                           gggTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACttttt      >  2:2911186/1‑150 (MQ=255)
                                                                                                                                            ggTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACtttttt     <  2:1130335/150‑1 (MQ=255)
                                                                                                                                             gTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTtta    >  1:3369542/1‑150 (MQ=255)
                                                                                                                                              tAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTttat   <  1:2327732/150‑1 (MQ=255)
                                                                                                                                               aaCCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTttatt  >  2:3993720/1‑150 (MQ=255)
                                                                                                                                               aaCCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCAACAGAATTGACTCCAGCCCGTGCAGCAACCAGCCCACTTTtataat  >  1:4825867/1‑148 (MQ=255)
                                                                                                                                               aaCCTGCAGGGATTGCGTCATCACCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTttatt  <  1:1522365/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGCAGTAAATCCTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTATT  >  CP009273/329225‑329517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑