Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,996,346 T→A 100% I70F (ATC→TTC)  yigG ← PRK11371 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,996,3460TA98.2% 553.0 / ‑0.2 169I70F (ATC→TTC) yigGPRK11371 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base A (75/91);  total (75/94)
Fisher's exact test for biased strand distribution p-value = 2.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 174 total aligned reads displayed.

AAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAATG  >  CP009273/3996200‑3996492
                                                                                                                                                  |                                                                                                                                                  
    aTTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCa                                                                                                                                             <  2:566146/150‑1 (MQ=255)
    aTTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCa                                                                                                                                             <  1:540003/150‑1 (MQ=255)
       aGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTa                                                                                                                                          <  1:3670886/150‑1 (MQ=255)
       aGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTa                                                                                                                                          >  2:4395859/1‑150 (MQ=255)
         cATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATc                                                                                                                                        <  1:4931446/150‑1 (MQ=255)
         cATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATc                                                                                                                                        <  1:1874752/150‑1 (MQ=255)
         cATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATc                                                                                                                                        <  1:1874826/150‑1 (MQ=255)
          aTACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCa                                                                                                                                       <  1:3224422/150‑1 (MQ=255)
          aTACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCa                                                                                                                                       <  2:1832273/150‑1 (MQ=255)
            aCTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACg                                                                                                                                     <  1:3581171/150‑1 (MQ=255)
             cTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACgg                                                                                                                                    <  2:4878651/150‑1 (MQ=255)
             cTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACgg                                                                                                                                    <  2:2318761/150‑1 (MQ=255)
              tGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGa                                                                                                                                   <  2:1436423/150‑1 (MQ=255)
               gAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAACACATAAGTTTTTATATAGAAGAAACCACTATCACGGAg                                                                                                                                  >  2:3845312/1‑150 (MQ=255)
               gAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAg                                                                                                                                  >  2:2036847/1‑150 (MQ=255)
               gAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAg                                                                                                                                  <  2:10223/150‑1 (MQ=255)
                aaaGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGt                                                                                                                                 >  1:824081/1‑150 (MQ=255)
                 aaGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTc                                                                                                                                <  1:5142182/150‑1 (MQ=255)
                 aaGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTc                                                                                                                                <  2:1112029/150‑1 (MQ=255)
                 aaGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTc                                                                                                                                <  2:3127807/150‑1 (MQ=255)
                 aaGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTc                                                                                                                                <  2:2975118/150‑1 (MQ=255)
                 aaGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTc                                                                                                                                >  1:2366495/1‑150 (MQ=255)
                 aaGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTc                                                                                                                                >  1:2366074/1‑150 (MQ=255)
                  aGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATAGCCTGAACAGGCAAAAACTACGGCTATCAATGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCg                                                                                                                               >  1:3595845/1‑150 (MQ=255)
                   gTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGc                                                                                                                              >  1:3331966/1‑150 (MQ=255)
                    ttgAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTAACAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCt                                                                                                                             <  2:504352/148‑1 (MQ=255)
                      gAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTgg                                                                                                                           <  2:4795925/150‑1 (MQ=255)
                         aaaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCaa                                                                                                                        >  1:272197/1‑150 (MQ=255)
                          aaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAt                                                                                                                       >  1:3917662/1‑150 (MQ=255)
                          aaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAt                                                                                                                       >  1:101260/1‑150 (MQ=255)
                              aCAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCAt                                                                                                                   >  2:3855007/1‑150 (MQ=255)
                                aaaTCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGt                                                                                                                 >  2:2735656/1‑150 (MQ=255)
                                aaaTCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGt                                                                                                                 >  2:2735812/1‑150 (MQ=255)
                                aaaTCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGt                                                                                                                 <  2:1311609/150‑1 (MQ=255)
                                      aaaaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtgatga                                                                                                           >  1:4015118/1‑150 (MQ=255)
                                       aaaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACTGAGTCGCTGGCAATTCATGTTGATGAc                                                                                                          >  1:4181153/1‑150 (MQ=255)
                                       aaaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGAc                                                                                                          >  2:1993769/1‑150 (MQ=255)
                                       aaaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGAc                                                                                                          <  2:4532890/150‑1 (MQ=255)
                                          aaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACgag                                                                                                       <  2:3935854/150‑1 (MQ=255)
                                           atcatcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACgaga                                                                                                      <  1:4356002/150‑1 (MQ=255)
                                           atcatcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACgaga                                                                                                      >  1:943073/1‑150 (MQ=255)
                                           atcatcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACgaga                                                                                                      <  1:1568947/150‑1 (MQ=255)
                                                cGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATg                                                                                                 <  2:3172021/150‑1 (MQ=255)
                                                 gAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATgg                                                                                                >  1:24366/1‑150 (MQ=255)
                                                    ccATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAAt                                                                                                  >  1:887067/1‑145 (MQ=255)
                                                    ccATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAAt                                                                                                  <  2:887067/145‑1 (MQ=255)
                                                     cATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGTCGAGATAATGGAGTa                                                                                            >  2:4500292/1‑150 (MQ=255)
                                                     cATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTa                                                                                            >  1:4596030/1‑150 (MQ=255)
                                                       ttGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATTGAGTACg                                                                                          <  1:2940184/150‑1 (MQ=255)
                                                       ttGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACg                                                                                          <  2:2425125/150‑1 (MQ=255)
                                                        tGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGa                                                                                         >  1:3239395/1‑150 (MQ=255)
                                                            tGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGt                                                                                     >  1:5164041/1‑150 (MQ=255)
                                                                 aGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCGATTCATGTTGATGACGAGATACTGGAGTCCGATGGTAgaga                                                                                >  2:2953244/1‑150 (MQ=255)
                                                                 aGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAgaga                                                                                >  2:2492505/1‑150 (MQ=255)
                                                                  ggCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGAc                                                                               >  2:4133708/1‑150 (MQ=255)
                                                                      aaaTCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACtata                                                                           <  1:4121079/150‑1 (MQ=255)
                                                                      aaaTCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACtata                                                                           <  1:4121045/150‑1 (MQ=255)
                                                                       aaTCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATaa                                                                          >  2:792305/1‑150 (MQ=255)
                                                                              ggCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGaaa                                                                   <  1:2916439/150‑1 (MQ=255)
                                                                                cTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGc                                                                 <  2:4116460/150‑1 (MQ=255)
                                                                                cTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGc                                                                 >  2:2858587/1‑150 (MQ=255)
                                                                                           atgatAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCa                                                      <  1:4131078/150‑1 (MQ=255)
                                                                                            ttatAGAGATGATATATACTGCTAATGTAACAAAAACATAAGTTTTTATATAGAAGAAACCCCTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCAt                                                     <  1:4484821/148‑1 (MQ=255)
                                                                                               tAGAGATGATATATCCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTCTCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGt                                                  <  2:3504579/150‑1 (MQ=255)
                                                                                                agagATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGtt                                                 >  1:279626/1‑150 (MQ=255)
                                                                                                 gagaTGATATATACTGCTAATGTACCAAACACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTa                                                <  1:2274888/150‑1 (MQ=255)
                                                                                                 gagaTGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTa                                                >  1:2173016/1‑150 (MQ=255)
                                                                                                  agaTGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaa                                               <  2:2200603/150‑1 (MQ=255)
                                                                                                    atgatATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaaa                                             <  2:4059544/150‑1 (MQ=255)
                                                                                                     tgatATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaaaa                                            >  2:4121539/1‑150 (MQ=255)
                                                                                                     tgatATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaaaa                                            >  1:4710324/1‑150 (MQ=255)
                                                                                                       atatatACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAg                                          <  1:1076871/150‑1 (MQ=255)
                                                                                                       atatatACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAg                                          >  1:1341112/1‑150 (MQ=255)
                                                                                                          tataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGata                                       >  1:1156763/1‑150 (MQ=255)
                                                                                                             aCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGataata                                    <  2:4133835/150‑1 (MQ=255)
                                                                                                              cTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGAtaataa                                   <  1:1132452/150‑1 (MQ=255)
                                                                                                                gCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGTAGTCGCTGGAAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGAtaataata                                 <  2:4820465/150‑3 (MQ=255)
                                                                                                                 cTATTGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGTAGTTCGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGaa                                <  2:3595845/150‑1 (MQ=255)
                                                                                                                 cTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGaa                                <  1:3044664/150‑1 (MQ=255)
                                                                                                                  tAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGaaa                               >  1:4115603/1‑150 (MQ=255)
                                                                                                                   aaTGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAg                              <  1:558667/150‑1 (MQ=255)
                                                                                                                    aTGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAgg                             <  2:1459700/150‑1 (MQ=255)
                                                                                                                      gTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCa                           <  1:3907402/150‑1 (MQ=255)
                                                                                                                      gTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCa                           <  1:1413813/150‑1 (MQ=255)
                                                                                                                        aCCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCaaa                         >  1:2830855/1‑150 (MQ=255)
                                                                                                                         ccAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCaaaa                        <  2:2605074/150‑1 (MQ=255)
                                                                                                                           aaaaaCATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAAt                      <  1:4417034/150‑1 (MQ=255)
                                                                                                                            aaaaCATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATg                     <  1:2737160/150‑1 (MQ=255)
                                                                                                                            aaaaCATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAGTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATg                     <  2:1559798/150‑1 (MQ=255)
                                                                                                                              aaCATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGaa                   <  1:4758313/150‑1 (MQ=255)
                                                                                                                               aCATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGaaa                  <  1:3487936/150‑1 (MQ=255)
                                                                                                                               aCATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGaaa                  <  1:2610089/150‑1 (MQ=255)
                                                                                                                                cATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAAt                 >  2:3800916/1‑150 (MQ=255)
                                                                                                                                       ttttATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTaa          >  2:2152427/1‑150 (MQ=255)
                                                                                                                                        tttATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTaaa         <  2:2787735/150‑1 (MQ=255)
                                                                                                                                          tatataGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTGAAAAAAGATAATAAGAAAGGCAACAATGAAAATTATTAaaat       >  2:4818604/1‑150 (MQ=255)
                                                                                                                                            tataGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAATGCAAAAATGAAATTTATTAaaataa     >  1:4807915/1‑150 (MQ=255)
                                                                                                                                              taGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACTAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAaaataaat   >  2:1232434/1‑150 (MQ=255)
                                                                                                                                               agaagaAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAATg  <  1:3855007/150‑1 (MQ=255)
                                                                                                                                               agaagaAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAATg  >  2:520327/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAATG  >  CP009273/3996200‑3996492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑