BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,211,751	G→T	5.0%	A222E (GCA→GAA)	arpA ←	ankyrin repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,211,751	0	G	T	5.0%	805.1 / 13.8	218	A222E (GCA→GAA)	arpA	ankyrin repeat protein
Reads supporting (aligned to +/- strand): ref base G (100/107); new base T (4/7); total (104/114)
Fisher's exact test for biased strand distribution p-value = 5.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 219 total aligned reads displayed.

TACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGGACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTG  >  CP009273/4211603‑4211900

taCATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGc                                                                                                                                                      <  1:2209190/150‑1 (MQ=255)

 aCATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCt                                                                                                                                                     <  1:2412903/150‑1 (MQ=255)

   aTTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCttt                                                                                                                                                   <  1:2233152/150‑1 (MQ=255)

    ttAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCttta                                                                                                                                                  <  1:4253089/150‑2 (MQ=255)

      aGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCAt                                                                                                                                                >  2:112922/1‑150 (MQ=255)

      aGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCAt                                                                                                                                                <  2:2910561/150‑1 (MQ=255)

        gATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATaa                                                                                                                                              <  1:4548478/150‑1 (MQ=255)

          tCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACt                                                                                                                                            >  2:2543509/1‑150 (MQ=255)

              aaaCCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGcc                                                                                                                                        <  2:1078728/150‑1 (MQ=255)

               aaCCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGccc                                                                                                                                       >  1:909512/1‑150 (MQ=255)

                 ccGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGa                                                                                                                                     <  2:2419212/150‑1 (MQ=255)

                  cGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGaa                                                                                                                                    <  1:3225949/150‑1 (MQ=255)

                  cGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGaa                                                                                                                                    <  1:5110916/150‑1 (MQ=255)

                    aaaCCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCAATCTTGTCAAAATATGCTTTCATAACTAGCACGAAAt                                                                                                                                  >  1:2147141/1‑150 (MQ=255)

                          cGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCgc                                                                                                                            <  1:742410/150‑1 (MQ=255)

                            tAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCgcag                                                                                                                          >  2:4608092/1‑150 (MQ=255)

                             aaTGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCgcagg                                                                                                                         <  2:1298694/150‑1 (MQ=255)

                                gggTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCgcaggcag                                                                                                                      >  1:4076162/1‑150 (MQ=255)

                                gggTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCgcaggcag                                                                                                                      >  1:4076054/1‑150 (MQ=255)

                                 ggTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGgcagc                                                                                                                     <  1:4507556/150‑1 (MQ=255)

                                  gTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGgcagca                                                                                                                    >  1:4468556/1‑150 (MQ=255)

                                    aCATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAgcaac                                                                                                                  >  2:3525087/1‑150 (MQ=255)

                                     cATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAgcaacg                                                                                                                 >  1:5122410/1‑150 (MQ=255)

                                     cATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAgcaacg                                                                                                                 <  2:2812981/150‑1 (MQ=255)

                                           tCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCaacgcaacgc                                                                                                           >  1:3618641/1‑150 (MQ=255)

                                             aGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGccc                                                                                                         <  2:892642/150‑1 (MQ=255)

                                               ttGTGTTCTTTATTCTTAGCCTGACCGTCAAAGCTTGGTTATCCACCATTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATATTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCt                                                                                                       <  1:3446077/150‑1 (MQ=255)

                                               ttGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCTACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCt                                                                                                       <  2:3854063/150‑1 (MQ=255)

                                               ttGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCt                                                                                                       >  2:2448659/1‑150 (MQ=255)

                                               ttGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCt                                                                                                       <  2:495417/150‑1 (MQ=255)

                                               ttGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCt                                                                                                       <  2:545911/150‑1 (MQ=255)

                                                tgtgTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATTCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTc                                                                                                      <  2:3146973/150‑1 (MQ=255)

                                                  tgtTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAg                                                                                                    >  2:1116675/1‑150 (MQ=255)

                                                  tgtTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAg                                                                                                    >  1:625766/1‑150 (MQ=255)

                                                   gtTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCAATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAga                                                                                                   >  2:4903242/1‑149 (MQ=255)

                                                      cTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACg                                                                                                <  2:4907427/150‑1 (MQ=255)

                                                          aTTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTg                                                                                            <  2:3969442/150‑1 (MQ=255)

                                                           ttCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTgg                                                                                           >  2:1897698/1‑150 (MQ=255)

                                                            tCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGa                                                                                          >  2:2666389/1‑150 (MQ=255)

                                                             cTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGaa                                                                                         >  2:2099899/1‑150 (MQ=255)

                                                             cTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGaa                                                                                         >  1:337261/1‑150 (MQ=255)

                                                             cTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGaa                                                                                         >  1:1286993/1‑150 (MQ=255)

                                                              ttAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGaaa                                                                                        <  2:4067635/150‑1 (MQ=255)

                                                               tAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATTCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAAc                                                                                       >  1:1140097/1‑150 (MQ=255)

                                                                aGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATTCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAAcc                                                                                      <  1:5058480/150‑1 (MQ=255)

                                                                aGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACTCAACGCCCCTCAGAACGGCTGGAAAcc                                                                                      <  1:3053469/150‑1 (MQ=255)

                                                                aGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAAcc                                                                                      <  2:909512/150‑1 (MQ=255)

                                                                aGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAAcc                                                                                      >  1:1538438/1‑150 (MQ=255)

                                                                  ccGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCaa                                                                                    <  1:4806155/150‑1 (MQ=255)

                                                                   cGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAAc                                                                                   <  1:654887/150‑1 (MQ=255)

                                                                      aCCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGcc                                                                                <  2:2432393/150‑1 (MQ=255)

                                                                        cGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCaa                                                                              >  2:81496/1‑150 (MQ=255)

                                                                         gTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCaaa                                                                             >  2:4910215/1‑150 (MQ=255)

                                                                         gTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCaaa                                                                             >  2:2045418/1‑150 (MQ=255)

                                                                          tCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAg                                                                            >  1:1959490/1‑150 (MQ=255)

                                                                           cAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGt                                                                           >  1:3552721/1‑150 (MQ=255)

                                                                           cAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGt                                                                           >  1:2053264/1‑150 (MQ=255)

                                                                              aGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTg                                                                        <  1:2428909/150‑1 (MQ=255)

                                                                               gCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTgg                                                                       <  1:2551583/150‑1 (MQ=255)

                                                                               gCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTgg                                                                       <  2:1339486/150‑1 (MQ=255)

                                                                                cATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGt                                                                      <  2:3788426/150‑1 (MQ=255)

                                                                                cATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGt                                                                      <  1:102067/150‑1 (MQ=255)

                                                                                    gTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGttttt                                                                  >  2:1563147/1‑150 (MQ=255)

                                                                                    gTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGttttt                                                                  >  1:2310092/1‑150 (MQ=255)

                                                                                     ttATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGTAAACCAACGCCAAAGTCTGGTTTTTg                                                                 <  2:607678/150‑1 (MQ=255)

                                                                                     ttATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTg                                                                 <  1:2802888/150‑1 (MQ=255)

                                                                                          caccaGTAATATGGCTGCCATAACACTGATGCCATTTGATACCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCAttt                                                            <  1:3866793/150‑1 (MQ=255)

                                                                                              aGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATTCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTttg                                                        <  2:515267/150‑1 (MQ=255)

                                                                                              aGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATTCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTttg                                                        <  2:2293407/150‑1 (MQ=255)

                                                                                                tAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTttgtt                                                      <  1:3048360/150‑1 (MQ=255)

                                                                                                 aaTATGTCTGCGATATCTCTGATGACTTTTGATTCCATCTTGTCAAAATATGCTTTCATATCTAGCGCGATATCACCGCAGGCAGCAACGCAACGCCCCTCTGAAGGGCTGGTTTCGTTCGCCAAAGTCTGGTTTTTGGATTTTttgttg                                                     <  1:4750273/150‑1 (MQ=255)

                                                                                                  atatGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGa                                                    <  2:5091776/150‑1 (MQ=255)

                                                                                                    atGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAg                                                  >  1:1070034/1‑150 (MQ=255)

                                                                                                     tGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGt                                                 >  1:607415/1‑150 (MQ=255)

                                                                                                      ggCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTg                                                <  2:2609224/150‑1 (MQ=255)

                                                                                                      ggCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTg                                                >  2:1893346/1‑150 (MQ=255)

                                                                                                         tGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGt                                             >  1:417639/1‑150 (MQ=255)

                                                                                                          gCCATAACACTGATGCCATTTGATTCCATCTTGACAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTg                                            >  1:4742618/1‑150 (MQ=255)

                                                                                                           ccATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTga                                           >  2:4855905/1‑150 (MQ=255)

                                                                                                            cATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCCGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCGGGGTGTTGCATTTTGTGGGGAAGTGCGTgag                                          >  2:2628997/1‑150 (MQ=255)

                                                                                                              gaaCACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGaa                                        <  2:2522168/149‑1 (MQ=255)

                                                                                                                     gATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTc                                 >  2:3635413/1‑150 (MQ=255)

                                                                                                                     gATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTc                                 >  1:487805/1‑150 (MQ=255)

                                                                                                                      aTGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCa                                >  2:4578502/1‑150 (MQ=255)

                                                                                                                       tGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAg                               >  1:3964581/1‑150 (MQ=255)

                                                                                                                        gCCATTTGATTCCATCTTGTCAAAATATTCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGc                              >  1:618031/1‑150 (MQ=255)

                                                                                                                         ccATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCt                             <  2:4529504/150‑1 (MQ=255)

                                                                                                                         ccATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCt                             <  2:1959490/150‑1 (MQ=255)

                                                                                                                           aTTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGa                           <  1:2662431/150‑1 (MQ=255)

                                                                                                                             ttGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAag                         <  2:4443496/150‑1 (MQ=255)

                                                                                                                             ttGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAag                         <  1:4335216/150‑1 (MQ=255)

                                                                                                                                 ttCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTa                     <  1:2455318/150‑1 (MQ=255)

                                                                                                                                 ttCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTa                     >  1:1838764/1‑150 (MQ=255)

                                                                                                                                  tCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTaa                    <  2:1630042/150‑1 (MQ=255)

                                                                                                                                            cAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGAt          >  2:3031227/1‑150 (MQ=255)

                                                                                                                                            cAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGAt          >  1:2691081/1‑150 (MQ=255)

                                                                                                                                             aaaaTATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATg         >  2:332759/1‑150 (MQ=255)

                                                                                                                                              aaaTATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATgg        >  1:132520/1‑150 (MQ=255)

                                                                                                                                                   tGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGttt   >  1:842222/1‑150 (MQ=255)

                                                                                                                                                    gCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTg  <  2:4872693/150‑1 (MQ=255)

TACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGGACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTG  >  CP009273/4211603‑4211900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`