Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,230,913 A→T 100% W424R (TGG→AGG)  xylE ← D‑xylose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,230,9130AT98.9% 922.1 / ‑0.9 274W424R (TGG→AGG) xylED‑xylose transporter
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base T (145/126);  total (147/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 274 total aligned reads displayed.

CTCAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCG  >  CP009273/4230767‑4231059
                                                                                                                                                  |                                                                                                                                                  
ctcAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGga                                                                                                                                                 >  2:172808/1‑150 (MQ=255)
  cAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGgaga                                                                                                                                               >  2:4202273/1‑150 (MQ=255)
   aaGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGAc                                                                                                                                              <  1:4457135/150‑1 (MQ=255)
    tgggTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACg                                                                                                                                             >  2:3144107/2‑150 (MQ=255)
       gTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGACGGTCCTGGAGACGAag                                                                                                                                          >  2:1172674/1‑150 (MQ=255)
       gTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAag                                                                                                                                          >  2:1076412/1‑150 (MQ=255)
        ttttACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAagt                                                                                                                                         <  2:3829363/150‑1 (MQ=255)
        ttttACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAagt                                                                                                                                         <  2:2768927/150‑1 (MQ=255)
         tttACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAagta                                                                                                                                        >  1:2189324/1‑150 (MQ=255)
         tttACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAagta                                                                                                                                        >  2:243938/1‑150 (MQ=255)
         tttACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAagta                                                                                                                                        >  1:3664409/1‑150 (MQ=255)
          ttACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAagtag                                                                                                                                       >  1:1835287/1‑150 (MQ=255)
          ttACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAagtag                                                                                                                                       >  1:1174744/1‑150 (MQ=255)
               tttGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCgc                                                                                                                                  >  2:2893534/1‑150 (MQ=255)
               tttGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCgc                                                                                                                                  >  2:156369/1‑150 (MQ=255)
                 tGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCgcca                                                                                                                                >  2:4139048/1‑150 (MQ=255)
                  ggTTTCCGGGACAAATTTCCACATACACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCgccag                                                                                                                               <  1:3905013/150‑1 (MQ=255)
                     ttCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCgccagcca                                                                                                                            <  1:754777/150‑1 (MQ=255)
                     ttCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGATTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCgccagcca                                                                                                                            >  1:2661428/1‑150 (MQ=255)
                        tgggACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTg                                                                                                                         >  2:943004/2‑150 (MQ=255)
                         gggACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTgg                                                                                                                        <  2:577048/150‑1 (MQ=255)
                         gggACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTgg                                                                                                                        <  1:4436768/150‑1 (MQ=255)
                          ggACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTggg                                                                                                                       >  1:3533325/1‑150 (MQ=255)
                           gACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGgc                                                                                                                      >  1:1038460/1‑150 (MQ=255)
                           gACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGgc                                                                                                                      >  1:5120522/1‑150 (MQ=255)
                              aaaTTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGgccgc                                                                                                                   >  1:3240395/1‑150 (MQ=255)
                               aaTTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGgccgcc                                                                                                                  >  1:2133380/1‑150 (MQ=255)
                                  ttCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCAcc                                                                                                               <  1:275135/150‑1 (MQ=255)
                                  ttCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCAcc                                                                                                               <  2:661276/150‑1 (MQ=255)
                                   tCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCAcag                                                                                                              <  1:1208964/150‑3 (MQ=255)
                                    ccACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACcgc                                                                                                             <  2:4783090/150‑1 (MQ=255)
                                    ccACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACcgc                                                                                                             >  2:4160377/1‑150 (MQ=255)
                                    ccACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACcgc                                                                                                             >  1:3760557/1‑150 (MQ=255)
                                           aaCAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGcc                                                                                                      <  2:892315/150‑1 (MQ=255)
                                                 gctgcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCt                                                                                                >  2:2592613/1‑150 (MQ=255)
                                                 gctgcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCt                                                                                                <  2:3435800/150‑1 (MQ=255)
                                                   tgcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGACGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCttt                                                                                              >  2:2383329/1‑150 (MQ=255)
                                                   tgcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCttt                                                                                              >  1:4851900/1‑150 (MQ=255)
                                                   tgcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATAGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCttt                                                                                              >  1:569996/1‑150 (MQ=255)
                                                     ccAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTAc                                                                                            <  1:4708617/150‑1 (MQ=255)
                                                       aGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCCGCGCTTTACCa                                                                                          >  2:3519855/1‑150 (MQ=255)
                                                       aGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCa                                                                                          >  1:1419298/1‑150 (MQ=255)
                                                        gAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCAc                                                                                         <  1:4214284/150‑1 (MQ=255)
                                                            gCCCATACAACCGTAAATCCAGTATGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAAt                                                                                     <  1:1979690/150‑1 (MQ=255)
                                                            gCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAAt                                                                                     <  2:331189/150‑1 (MQ=255)
                                                              ccATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAg                                                                                   >  2:2258850/1‑150 (MQ=255)
                                                                aTACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCa                                                                                 >  1:1217808/1‑150 (MQ=255)
                                                                aTACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCa                                                                                 >  2:2130629/1‑150 (MQ=255)
                                                                 tACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCAt                                                                                <  2:4312385/150‑1 (MQ=255)
                                                                   cAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTc                                                                              <  2:27848/150‑1 (MQ=255)
                                                                     aCCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCgg                                                                            <  2:4507353/150‑1 (MQ=255)
                                                                        gTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGaa                                                                         >  1:243330/1‑150 (MQ=255)
                                                                         tAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAg                                                                        >  2:5022601/1‑150 (MQ=255)
                                                                             tCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGAttt                                                                    <  1:86368/150‑1 (MQ=255)
                                                                              ccAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTc                                                                   >  1:4736306/1‑150 (MQ=255)
                                                                              ccAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTc                                                                   <  1:2592613/150‑1 (MQ=255)
                                                                              ccAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTc                                                                   <  2:4026266/150‑1 (MQ=255)
                                                                               cAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTcc                                                                  >  1:4623983/1‑150 (MQ=255)
                                                                               cAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTcc                                                                  >  1:815109/1‑150 (MQ=255)
                                                                                aGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCg                                                                 >  1:2932894/1‑150 (MQ=255)
                                                                                 gTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGa                                                                >  2:40588/1‑150 (MQ=255)
                                                                                  tAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAc                                                               <  1:4902098/150‑1 (MQ=255)
                                                                                    ggAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAcag                                                             <  2:205796/150‑1 (MQ=255)
                                                                                      agaAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAcagca                                                           >  1:2628147/1‑150 (MQ=255)
                                                                                      agaAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAcagca                                                           <  2:3162638/150‑1 (MQ=255)
                                                                                       gaAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAcagcag                                                          >  1:1676828/1‑150 (MQ=255)
                                                                                       gaAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAcagcag                                                          <  1:5021401/150‑1 (MQ=255)
                                                                                         aaCCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCACCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTa                                                        <  1:410584/150‑1 (MQ=255)
                                                                                            cGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTAccc                                                     <  2:2073114/150‑1 (MQ=255)
                                                                                            cGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTAccc                                                     >  2:1312799/1‑150 (MQ=255)
                                                                                                 tgGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCAt                                                >  1:1472630/1‑150 (MQ=255)
                                                                                                    aaaTGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATAcc                                             <  2:714140/150‑1 (MQ=255)
                                                                                                     aaTGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCg                                            <  2:5003865/150‑1 (MQ=255)
                                                                                                        gggCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGAc                                         <  1:1088772/150‑1 (MQ=255)
                                                                                                        gggCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGAc                                         >  1:2024017/1‑150 (MQ=255)
                                                                                                           ccaccaGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGAcccc                                      >  1:2316747/1‑150 (MQ=255)
                                                                                                           ccaccaGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGAcccc                                      <  2:4223490/150‑1 (MQ=255)
                                                                                                             accaGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAg                                    >  1:1294817/1‑150 (MQ=255)
                                                                                                             accaGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAg                                    >  1:3027292/1‑150 (MQ=255)
                                                                                                               cagccagGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGa                                  >  1:1967065/1‑150 (MQ=255)
                                                                                                                agccagGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGAc                                 <  2:822150/150‑1 (MQ=255)
                                                                                                                  ccagGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACAt                               >  1:3377224/1‑150 (MQ=255)
                                                                                                                  ccagGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACAt                               <  2:2199176/150‑1 (MQ=255)
                                                                                                                    agGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATgg                             <  2:1780811/150‑1 (MQ=255)
                                                                                                                    agGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATgg                             >  2:4961628/1‑150 (MQ=255)
                                                                                                                         tttttGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAg                        <  2:156997/150‑1 (MQ=255)
                                                                                                                            ttGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAggcg                     <  2:3749879/150‑1 (MQ=255)
                                                                                                                             tGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAggcgg                    >  2:5055451/1‑150 (MQ=255)
                                                                                                                              gTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAggcggc                   <  2:3876683/150‑1 (MQ=255)
                                                                                                                               tCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCa                  <  1:1723194/150‑1 (MQ=255)
                                                                                                                               tCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCa                  <  1:943004/150‑1 (MQ=255)
                                                                                                                               tCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCa                  >  1:3282235/1‑150 (MQ=255)
                                                                                                                                 catcatCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAAc                >  2:3554212/1‑150 (MQ=255)
                                                                                                                                   tcatcGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACAt              <  2:3597067/150‑1 (MQ=255)
                                                                                                                                   tcatcGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACAt              <  2:1287649/150‑1 (MQ=255)
                                                                                                                                     atcGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAg            >  2:3775564/1‑150 (MQ=255)
                                                                                                                                        gggAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAAc         >  2:1328325/1‑150 (MQ=255)
                                                                                                                                         ggAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCCGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACa        <  2:4741549/150‑1 (MQ=255)
                                                                                                                                           aaGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGc      >  1:2542974/1‑150 (MQ=255)
                                                                                                                                              gTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATc   <  2:3412064/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTCAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCG  >  CP009273/4230767‑4231059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑