BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,249,117	G→T	100%	intergenic (+68/‑48)	dinF → / → yjbJ	oxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein/stress‑induced protein, UPF0337 family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,249,117	0	G	T	99.1%	743.8 / ‑2.8	226	intergenic (+68/‑48)	dinF/yjbJ	oxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein/stress‑induced protein, UPF0337 family
Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (104/120); total (105/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 230 total aligned reads displayed.

TTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGA  >  CP009273/4248970‑4249262

ttCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAgaataa                                                                                                                                                 >  1:5148620/1‑150 (MQ=255)

  cTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATaaaa                                                                                                                                               >  2:2457837/1‑150 (MQ=255)

  cTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATaaaa                                                                                                                                               <  1:812443/150‑1 (MQ=255)

  cTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATaaaa                                                                                                                                               <  2:4446298/150‑1 (MQ=255)

   tGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAg                                                                                                                                              <  2:2940708/150‑1 (MQ=255)

      cGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGa                                                                                                                                           >  2:3822593/1‑150 (MQ=255)

         tGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACg                                                                                                                                        <  1:1792974/150‑1 (MQ=255)

         tGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACg                                                                                                                                        <  2:3246812/150‑1 (MQ=255)

         tGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACg                                                                                                                                        <  2:3246934/150‑1 (MQ=255)

             cgGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTAct                                                                                                                                    >  2:4850352/1‑150 (MQ=255)

              gggCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTActc                                                                                                                                   >  1:3192475/1‑150 (MQ=255)

              gggCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTActc                                                                                                                                   >  1:2510751/1‑150 (MQ=255)

                gCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACtctc                                                                                                                                 <  1:4315340/150‑1 (MQ=255)

                gCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACtctc                                                                                                                                 <  1:4767233/150‑1 (MQ=255)

                gCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACtctc                                                                                                                                 <  1:953577/150‑1 (MQ=255)

                 cTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCa                                                                                                                                >  1:3625276/1‑150 (MQ=255)

                  tttCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCAt                                                                                                                               >  1:4633356/1‑150 (MQ=255)

                     ctctGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTca                                                                                                                            >  1:2558070/1‑150 (MQ=255)

                      tctGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTcac                                                                                                                           <  1:4251097/150‑1 (MQ=255)

                           cggcTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCt                                                                                                                      <  1:2586409/150‑1 (MQ=255)

                             gcTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTaa                                                                                                                    >  1:2967507/1‑150 (MQ=255)

                              cTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAAc                                                                                                                   <  2:4596661/150‑1 (MQ=255)

                              cTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAAc                                                                                                                   <  2:242955/150‑1 (MQ=255)

                              cTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAAc                                                                                                                   >  1:4905431/1‑150 (MQ=255)

                               tATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACg                                                                                                                  >  2:4719000/1‑150 (MQ=255)

                                 tCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGAt                                                                                                                <  2:2761565/150‑1 (MQ=255)

                                       ggcgTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTc                                                                                                          >  2:64960/1‑150 (MQ=255)

                                          gTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTg                                                                                                       >  2:2649340/1‑150 (MQ=255)

                                             aCTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGAtt                                                                                                    >  2:3531173/1‑150 (MQ=255)

                                               tGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTAt                                                                                                  <  2:1633753/150‑1 (MQ=255)

                                                   gcAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAAt                                                                                              >  1:3031408/1‑150 (MQ=255)

                                                   gcAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAAt                                                                                              <  1:896633/150‑1 (MQ=255)

                                                         gTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGAt                                                                                        >  2:4273936/1‑150 (MQ=255)

                                                          tACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATg                                                                                       <  2:949436/150‑1 (MQ=255)

                                                           aCCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGa                                                                                      <  1:4005254/150‑1 (MQ=255)

                                                             cTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAg                                                                                    <  1:4080927/150‑1 (MQ=255)

                                                             cTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAg                                                                                    <  1:358606/150‑1 (MQ=255)

                                                              tGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGc                                                                                   <  2:3754638/150‑1 (MQ=255)

                                                              tGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGc                                                                                   >  1:2077690/1‑150 (MQ=255)

                                                               ggTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGcc                                                                                  <  1:2344928/150‑1 (MQ=255)

                                                               ggTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGcc                                                                                  >  1:4877089/1‑150 (MQ=255)

                                                                 tttGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCcgg                                                                                <  2:4805367/150‑1 (MQ=255)

                                                                    gCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCcggcgg                                                                             >  1:3799398/1‑150 (MQ=255)

                                                                     ccGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGt                                                                            <  1:766665/150‑1 (MQ=255)

                                                                     ccGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGt                                                                            >  1:4994631/1‑150 (MQ=255)

                                                                      cGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTa                                                                           >  2:4541066/1‑150 (MQ=255)

                                                                      cGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTa                                                                           <  2:539601/150‑1 (MQ=255)

                                                                      cGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTa                                                                           >  2:132980/1‑150 (MQ=255)

                                                                       gCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTaa                                                                          <  1:586882/150‑1 (MQ=255)

                                                                       gCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTaa                                                                          <  1:3520545/150‑1 (MQ=255)

                                                                        cAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAAc                                                                         <  1:1151899/150‑1 (MQ=255)

                                                                        cAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAAc                                                                         >  2:2380067/1‑150 (MQ=255)

                                                                         aaCGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACt                                                                        >  1:333679/1‑150 (MQ=255)

                                                                          aCGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTg                                                                       <  1:1649983/150‑1 (MQ=255)

                                                                           cGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTgg                                                                      >  2:2369326/1‑150 (MQ=255)

                                                                            gTGACGGTTAAAAATTCTGAATAAATAATCATAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGa                                                                     <  1:4364453/150‑1 (MQ=255)

                                                                             tGACGGTTAAAAATTCTGAATAAATCATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCCCAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGaa                                                                    <  2:5087495/150‑1 (MQ=255)

                                                                              gACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTATACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGaaa                                                                   >  1:2486165/1‑150 (MQ=255)

                                                                                cGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACa                                                                 >  2:4407968/1‑150 (MQ=255)

                                                                                 ggTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAg                                                                >  1:1289481/1‑150 (MQ=255)

                                                                                 ggTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAg                                                                >  2:845308/1‑150 (MQ=255)

                                                                                  gTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGt                                                               >  2:2217076/1‑150 (MQ=255)

                                                                                   ttAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGtt                                                              <  1:1082514/150‑1 (MQ=255)

                                                                                       aaaTTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTaaa                                                          >  2:1998726/1‑150 (MQ=255)

                                                                                       aaaTTCTGAATAAATAATCATAAGCCAATTTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGATCGTACTCTCATTCACTACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTaaa                                                          <  2:4925753/150‑1 (MQ=255)

                                                                                         aTTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAgg                                                        <  2:4246144/150‑1 (MQ=255)

                                                                                            cTGACTAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTaa                                                     <  1:2638491/150‑1 (MQ=255)

                                                                                                  aaataaTCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGCAGCCGGCGGTAACTGGAAACAGGTTAAAGGTAAAGTGaa                                               >  2:4945245/1‑150 (MQ=255)

                                                                                                   aataatCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGaaa                                              <  2:4903905/150‑1 (MQ=255)

                                                                                                    ataatCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAag                                             >  2:3047554/1‑150 (MQ=255)

                                                                                                      aatCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAagag                                           <  1:2739071/150‑1 (MQ=255)

                                                                                                      aatCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAagag                                           <  2:2510751/150‑1 (MQ=255)

                                                                                                       atCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGc                                          >  2:1796250/1‑150 (MQ=255)

                                                                                                       atCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGc                                          <  1:2370135/150‑1 (MQ=255)

                                                                                                          cTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAAt                                       <  1:1622374/150‑1 (MQ=255)

                                                                                                           tAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATg                                      >  1:3283875/1‑150 (MQ=255)

                                                                                                           tAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATg                                      >  2:1422664/1‑150 (MQ=255)

                                                                                                              gCCAAATTGCTGTCTACACTTTATCTCACTTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGATGTCTTGATTATGAATAAAGATGAAGCCTGAGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATgggg                                   <  2:4763960/150‑1 (MQ=255)

                                                                                                              gCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGCAAAGTGAAAGAGCAATgggg                                   >  2:1524498/1‑150 (MQ=255)

                                                                                                               ccAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGACGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGc                                  >  2:4033882/1‑150 (MQ=255)

                                                                                                               ccAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGc                                  <  2:3088381/150‑1 (MQ=255)

                                                                                                               ccAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAAAGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGc                                  <  1:3857635/150‑1 (MQ=255)

                                                                                                                  aaTTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCaaa                               >  2:4208602/1‑150 (MQ=255)

                                                                                                                       cTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGAc                          <  2:2027471/150‑1 (MQ=255)

                                                                                                                        tGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGAcc                         >  1:3179355/1‑150 (MQ=255)

                                                                                                                        tGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGAcc                         <  2:2714477/150‑1 (MQ=255)

                                                                                                                        tGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGAcc                         <  2:1704561/150‑1 (MQ=255)

                                                                                                                        tGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGAcc                         <  1:3848315/150‑1 (MQ=255)

                                                                                                                        tGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGAcc                         >  2:1637890/1‑150 (MQ=255)

                                                                                                                         gACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCg                        >  1:1106325/1‑150 (MQ=255)

                                                                                                                             acacTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgatga                    >  2:4569225/1‑150 (MQ=255)

                                                                                                                               acTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgatgatg                  >  2:1541048/1‑150 (MQ=255)

                                                                                                                                 ttAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgatgatgat                >  2:1058482/1‑150 (MQ=255)

                                                                                                                                  tAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGata               <  2:4284641/150‑1 (MQ=255)

                                                                                                                                     tctcACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGa            >  1:544979/1‑150 (MQ=255)

                                                                                                                                      ctcACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGAc           <  1:1998726/150‑1 (MQ=255)

                                                                                                                                       tcACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGAcc          <  2:1475391/150‑2 (MQ=255)

                                                                                                                                        cACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGa         >  2:928488/1‑150 (MQ=255)

                                                                                                                                             tCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCAtt    >  2:2244761/1‑150 (MQ=255)

                                                                                                                                              cAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTg   <  1:2296270/150‑1 (MQ=255)

TTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGA  >  CP009273/4248970‑4249262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`