Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,266,318 G→T 100% R307L (CGT→CTT)  yjcC → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,266,3180GT98.5% 875.2 / 1.5 269R307L (CGT→CTT) yjcCputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (4/0);  new base T (140/125);  total (144/125)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 273 total aligned reads displayed.

TATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCT  >  CP009273/4266171‑4266467
                                                                                                                                                   |                                                                                                                                                     
tATTACTCTGGCTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACttt                                                                                                                                                     <  2:389050/150‑1 (MQ=255)
 aTTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTg                                                                                                                                                    >  1:727356/1‑150 (MQ=255)
  ttACTCAGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTAATACCAGCCAATAATCGAAATCAAAACATAAAAATGTATCGGAGATGAAGCGTTGTTACTTTgg                                                                                                                                                   >  2:219147/1‑150 (MQ=255)
      tctGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTg                                                                                                                                               <  1:2778318/150‑1 (MQ=255)
       ctGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTgg                                                                                                                                              <  2:588139/150‑1 (MQ=255)
            tACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGc                                                                                                                                         >  1:963665/1‑150 (MQ=255)
            tACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGc                                                                                                                                         >  2:3706291/1‑150 (MQ=255)
             aCGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCa                                                                                                                                        >  1:1985934/1‑150 (MQ=255)
                 aTTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAgggg                                                                                                                                    <  1:4577328/150‑1 (MQ=255)
                 aTTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAgggg                                                                                                                                    >  1:3322451/1‑150 (MQ=255)
                 aTTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAgggg                                                                                                                                    <  2:3322451/150‑1 (MQ=255)
                   tCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCa                                                                                                                                  <  1:1907981/150‑1 (MQ=255)
                   tCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCa                                                                                                                                  <  2:2141176/150‑1 (MQ=255)
                     gACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCaaa                                                                                                                                >  1:1150539/1‑150 (MQ=255)
                     gACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCaaa                                                                                                                                >  2:2242698/1‑150 (MQ=255)
                     gACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCaaa                                                                                                                                <  2:1702233/150‑1 (MQ=255)
                      aCAAAACTATTTATCTCCCAAACGTAAATTGCAACTCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAaat                                                                                                                               <  1:2368555/150‑1 (MQ=255)
                                 tATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCgg                                                                                                                    <  2:2099609/150‑1 (MQ=255)
                                   tctcCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCa                                                                                                                  <  2:4790830/150‑1 (MQ=255)
                                   tcacccAAACGTAAAGTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCCGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCa                                                                                                                  <  1:2010787/147‑1 (MQ=255)
                                      cccAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCagag                                                                                                               >  2:4482462/1‑150 (MQ=255)
                                        cAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGtt                                                                                                             >  2:4171384/1‑150 (MQ=255)
                                         aaaCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGttt                                                                                                            >  1:283238/1‑150 (MQ=255)
                                         aaaCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGttt                                                                                                            <  2:572701/150‑1 (MQ=255)
                                          aaCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTa                                                                                                           <  2:3845960/150‑1 (MQ=255)
                                          aaCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTa                                                                                                           >  2:1788291/1‑150 (MQ=255)
                                          aaCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTa                                                                                                           >  2:1087409/1‑150 (MQ=255)
                                               aaaTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCg                                                                                                      >  2:3815576/1‑150 (MQ=255)
                                                 aTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCt                                                                                                    <  2:4966030/150‑1 (MQ=255)
                                                      aaCGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAg                                                                                               >  2:2285138/1‑150 (MQ=255)
                                                        cgcgCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGaa                                                                                             <  1:627645/150‑1 (MQ=255)
                                                          cgcCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGaaaa                                                                                           >  2:3785703/1‑150 (MQ=255)
                                                           gcCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAag                                                                                          >  1:3107164/1‑150 (MQ=255)
                                                             ccTCGAAAAACATCAACTTTGTCTTTATTACCAGCCCATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAagga                                                                                        >  1:896034/1‑150 (MQ=255)
                                                             ccTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAagga                                                                                        <  1:4084191/150‑1 (MQ=255)
                                                              cTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAaggag                                                                                       >  1:4653440/1‑150 (MQ=255)
                                                                  aaaaaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGga                                                                                   <  1:1059437/150‑1 (MQ=255)
                                                                  aaaaaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGga                                                                                   >  2:5079243/1‑150 (MQ=255)
                                                                   aaaaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgat                                                                                  >  1:2778844/1‑150 (MQ=255)
                                                                    aaaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatg                                                                                 >  2:526575/1‑150 (MQ=255)
                                                                    aaaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatg                                                                                 >  1:1325876/1‑150 (MQ=255)
                                                                        atcatcTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCATATAATGAATCCGGCAGCGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAg                                                                             <  1:3881876/150‑1 (MQ=255)
                                                                          cAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGaa                                                                           <  1:347684/150‑1 (MQ=255)
                                                                          cAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGaa                                                                           >  2:309749/1‑150 (MQ=255)
                                                                            aCTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACa                                                                         >  1:3411105/1‑150 (MQ=255)
                                                                              tttGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGa                                                                       >  2:3191463/1‑150 (MQ=255)
                                                                              tttGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGa                                                                       <  1:1009536/150‑1 (MQ=255)
                                                                                 gTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATaa                                                                    <  1:2029317/150‑1 (MQ=255)
                                                                                   cTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACt                                                                  <  2:4713015/150‑1 (MQ=255)
                                                                                      tattaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAt                                                               >  1:4503661/1‑150 (MQ=255)
                                                                                      tattaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAt                                                               <  1:4959422/150‑1 (MQ=255)
                                                                                      tattaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAt                                                               >  2:2406408/1‑150 (MQ=255)
                                                                                       attaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAtt                                                              >  2:1574178/1‑150 (MQ=255)
                                                                                       attaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGAAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAtt                                                              >  1:3052890/1‑150 (MQ=255)
                                                                                        ttaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTa                                                             <  1:1760840/150‑1 (MQ=255)
                                                                                        ttaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTa                                                             <  1:1515156/150‑1 (MQ=255)
                                                                                         taCCAGCCAATAATCGATATCAAAACAGAAAAATGTAACGGCGCTGAAGCGTTGTTACTTTGGCATGGTGAGCAGGGGCAAATAAAGAATCCGGCAGAGTTTATTAATATGACAGAAATGAAGGGGATGAAAGAAAAGAAAACTGaaaaa                                                            >  2:2668944/1‑146 (MQ=255)
                                                                                                cAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTg                                                     <  2:3517668/150‑1 (MQ=255)
                                                                                                cAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTg                                                     <  2:2630599/150‑1 (MQ=255)
                                                                                                  ataatCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGAt                                                   >  2:300182/1‑150 (MQ=255)
                                                                                                   taatCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATa                                                  >  2:5000821/1‑150 (MQ=255)
                                                                                                       cGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGt                                              <  2:2847463/150‑1 (MQ=255)
                                                                                                       cGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGt                                              >  1:241254/1‑150 (MQ=255)
                                                                                                         atatCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCt                                            >  1:742586/1‑150 (MQ=255)
                                                                                                           atCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTc                                          <  1:1408559/150‑1 (MQ=255)
                                                                                                           atCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTc                                          >  2:1632887/1‑150 (MQ=255)
                                                                                                            tCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTcc                                         <  2:4308250/150‑1 (MQ=255)
                                                                                                                aaCAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGa                                     <  1:2108499/150‑1 (MQ=255)
                                                                                                                 aCAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGAt                                    <  1:5102721/150‑1 (MQ=255)
                                                                                                                  cAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATc                                   <  1:4746020/150‑1 (MQ=255)
                                                                                                                     aaaaaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTgg                                >  1:2613123/1‑150 (MQ=255)
                                                                                                                     aaaaaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTgg                                >  1:550607/1‑150 (MQ=255)
                                                                                                                       aaaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGc                              <  2:1325876/150‑1 (MQ=255)
                                                                                                                       aaaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGc                              <  1:828467/150‑1 (MQ=255)
                                                                                                                       aaaTGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGc                              >  2:2799064/1‑150 (MQ=255)
                                                                                                                         aTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGa                            >  2:4841425/1‑150 (MQ=255)
                                                                                                                         aTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGa                            >  1:3998643/1‑150 (MQ=255)
                                                                                                                         aTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGa                            <  1:4109528/150‑1 (MQ=255)
                                                                                                                          tGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGATTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACAGATTATGTTATTGATAATGTCTTCCGCGAACTGGGCGAt                           >  1:2942624/1‑150 (MQ=255)
                                                                                                                           gTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGAtt                          <  1:2564023/150‑1 (MQ=255)
                                                                                                                           gTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGAtt                          <  2:1764808/150‑1 (MQ=255)
                                                                                                                             aTCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTAc                        <  2:4581212/150‑1 (MQ=255)
                                                                                                                               tggCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCt                      >  1:3958440/2‑150 (MQ=255)
                                                                                                                               cGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCt                      >  2:4874276/1‑150 (MQ=255)
                                                                                                                               cGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCt                      <  2:961217/150‑1 (MQ=255)
                                                                                                                               cGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCt                      >  2:1694542/1‑150 (MQ=255)
                                                                                                                               cGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCt                      >  1:4361892/1‑150 (MQ=255)
                                                                                                                                 gcgcTTAAGCGTTGTGACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTgg                    <  1:3269551/150‑1 (MQ=255)
                                                                                                                                     tGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAac                <  2:3383575/150‑1 (MQ=255)
                                                                                                                                      gAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAaca               >  2:1338416/1‑150 (MQ=255)
                                                                                                                                        aGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAAcaca             <  1:4500712/150‑1 (MQ=255)
                                                                                                                                        aGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAAcaca             >  1:2339992/1‑150 (MQ=255)
                                                                                                                                        aGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAAcaca             <  1:218446/150‑1 (MQ=255)
                                                                                                                                         gCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACAt            <  1:5093735/150‑1 (MQ=255)
                                                                                                                                           gttgttACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGc          >  2:2977761/1‑150 (MQ=255)
                                                                                                                                            ttgttACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCa         <  2:3960498/150‑1 (MQ=255)
                                                                                                                                            ttgttACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCa         >  2:1108974/1‑150 (MQ=255)
                                                                                                                                            ttgttACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGAACTGGGCGATTACCTGGCAACACATGCa         <  2:2741803/150‑1 (MQ=255)
                                                                                                                                              gttACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGa       <  2:357064/150‑1 (MQ=255)
                                                                                                                                               ttACTTTGGCCTGGTGATCAGGGGCAATTATTGTATCCGGCAGAGTTTATTCCGCTGGCAGAAATGGTGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACATTGCAACACATGCAGAt      <  2:719258/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCT  >  CP009273/4266171‑4266467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑