Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,274,641 A→C 10.2% Y64D (TAC→GAC)  actP ← acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,274,6410AC10.2% 658.6 / 64.4 255Y64D (TAC→GAC) actPacetate transporter
Reads supporting (aligned to +/- strand):  ref base A (100/129);  new base C (18/8);  total (118/137)
Fisher's exact test for biased strand distribution p-value = 2.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 270 total aligned reads displayed.

GCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGA  >  CP009273/4274492‑4274790
                                                                                                                                                     |                                                                                                                                                     
gCCCACCCGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTgta                                                                                                                                                       <  1:1548790/150‑1 (MQ=255)
  ccaccaGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTgtagt                                                                                                                                                     >  2:148669/1‑150 (MQ=255)
   caccaGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTgtagta                                                                                                                                                    >  2:3145804/1‑150 (MQ=255)
    accaGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGtagtag                                                                                                                                                   <  2:205467/150‑1 (MQ=255)
       aGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCg                                                                                                                                                >  2:2285630/1‑150 (MQ=255)
         gAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCt                                                                                                                                              >  1:3694481/1‑150 (MQ=255)
          aaGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTa                                                                                                                                             <  2:3585753/150‑1 (MQ=255)
           aGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTAc                                                                                                                                            <  1:3843956/150‑1 (MQ=255)
              ccAGCGAGGAACTTAAGCCGTCAGAGCCGGAGGTAACCACCAGCGCGGAGATCCCCAAGACTGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACgag                                                                                                                                         <  2:4295944/150‑1 (MQ=255)
               cAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACgaga                                                                                                                                        <  1:4039931/150‑1 (MQ=255)
                aGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGaa                                                                                                                                       >  2:67173/1‑150 (MQ=255)
                 gCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAAc                                                                                                                                      <  1:1430740/150‑1 (MQ=255)
                 gCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAAc                                                                                                                                      <  1:270827/150‑1 (MQ=255)
                  cGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACg                                                                                                                                     >  1:4642641/1‑150 (MQ=255)
                   gAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTCGTAGTCGCTACGAGAACGt                                                                                                                                    >  1:3567555/1‑150 (MQ=255)
                     gTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTAc                                                                                                                                  >  1:2202073/1‑150 (MQ=255)
                      tAAATTAAGCAGTCATAGCCGGAGGTAAACACCAGCTCGGAGATCCCAAAGAATGAGGAGGCGGACATATAGTACCCGGCAATCGCCAGACCGTTCTGGAAGCCATTGATATTGCCGCCTGCGGTTTAGTAGTCGCTACGAGAACGTAcg                                                                                                                                 <  2:2934362/150‑1 (MQ=255)
                        aaTTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTAcgcg                                                                                                                               >  1:860709/1‑150 (MQ=255)
                          ttAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGtt                                                                                                                             <  2:27273/150‑1 (MQ=255)
                            aaGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGtttt                                                                                                                           <  2:3325958/150‑1 (MQ=255)
                            aaGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGtttt                                                                                                                           <  2:1180743/150‑1 (MQ=255)
                             aGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTg                                                                                                                          <  1:4988355/150‑1 (MQ=255)
                                cGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATg                                                                                                                       <  2:5049519/150‑1 (MQ=255)
                                    aTAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCa                                                                                                                   >  1:1628745/1‑150 (MQ=255)
                                      aGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGt                                                                                                                 <  2:1097125/150‑1 (MQ=255)
                                       gCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACTTACGCGTTTTGATGCCCAGTa                                                                                                                <  1:682585/150‑1 (MQ=255)
                                        ccGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAg                                                                                                               <  2:2896937/150‑1 (MQ=255)
                                         cGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAgg                                                                                                              <  1:4198602/150‑1 (MQ=255)
                                          ggAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGt                                                                                                             >  1:624021/1‑150 (MQ=255)
                                              gTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATg                                                                                                         <  2:2166102/150‑1 (MQ=255)
                                                aaaCACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTCGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGcc                                                                                                       >  2:3715677/1‑150 (MQ=255)
                                                aaaCACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGcc                                                                                                       <  2:507001/150‑1 (MQ=255)
                                                 aaCACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCg                                                                                                      >  2:550388/1‑150 (MQ=255)
                                                    acCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGc                                                                                                   <  1:2499354/150‑1 (MQ=255)
                                                      cAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGt                                                                                                 <  2:4567058/150‑1 (MQ=255)
                                                         cgcgGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTaaa                                                                                              >  2:4420867/1‑150 (MQ=255)
                                                          gcgGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAac                                                                                             <  1:4550486/150‑1 (MQ=255)
                                                           cgGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAaca                                                                                            >  2:4515464/1‑150 (MQ=255)
                                                               gaTCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACacgac                                                                                        <  2:2454013/150‑1 (MQ=255)
                                                                  ccccAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGaa                                                                                     <  1:4142245/150‑1 (MQ=255)
                                                                  ccccAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGaa                                                                                     <  1:1563859/150‑1 (MQ=255)
                                                                    ccAAGAATGAGGCGGCTGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACTTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGaaaa                                                                                   <  2:1586491/150‑1 (MQ=255)
                                                                    ccAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGaaaa                                                                                   <  1:3149618/150‑1 (MQ=255)
                                                                      aaGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATc                                                                                 >  1:814135/1‑150 (MQ=255)
                                                                         aaTGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAgg                                                                              >  2:1342461/1‑150 (MQ=255)
                                                                         aaTGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAgg                                                                              >  1:1159853/1‑150 (MQ=255)
                                                                             aGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTCGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACa                                                                          >  2:5071051/1‑150 (MQ=255)
                                                                              ggcggcGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACat                                                                         <  2:3840509/150‑1 (MQ=255)
                                                                                cggcggACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACataa                                                                       >  1:4167235/1‑150 (MQ=255)
                                                                                 ggcggACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACataat                                                                      <  2:3041914/150‑1 (MQ=255)
                                                                                   cggACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACAtaataa                                                                    <  2:4875611/150‑1 (MQ=255)
                                                                                    ggACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATaataat                                                                   >  2:333607/1‑150 (MQ=255)
                                                                                     gACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATc                                                                  <  2:115254/150‑1 (MQ=255)
                                                                                         tataGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCt                                                              <  2:2601090/150‑1 (MQ=255)
                                                                                          ataGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTg                                                             >  1:2710811/1‑150 (MQ=255)
                                                                                            aGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGcc                                                           <  1:3412512/150‑1 (MQ=255)
                                                                                             gTCCCCGGCAATCGCCAGCCCGTTCTGTAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACTTACGCGCTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCa                                                          <  2:3456819/150‑1 (MQ=255)
                                                                                             gTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCa                                                          <  2:3511593/150‑1 (MQ=255)
                                                                                                  cGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCg                                                     <  1:2565894/150‑1 (MQ=255)
                                                                                                   ggCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGt                                                    >  1:3269261/1‑150 (MQ=255)
                                                                                                    gCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGtt                                                   <  1:1363678/150‑1 (MQ=255)
                                                                                                     cAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTCGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTtg                                                  >  1:2611507/1‑150 (MQ=255)
                                                                                                     cAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTtg                                                  >  1:869552/1‑150 (MQ=255)
                                                                                                     cAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTtg                                                  >  1:523356/1‑150 (MQ=255)
                                                                                                      aaTCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTtgg                                                 <  1:1467952/150‑1 (MQ=255)
                                                                                                       aTCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTtggc                                                <  1:2080965/150‑1 (MQ=255)
                                                                                                        tCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTtggct                                               >  1:1642759/1‑150 (MQ=255)
                                                                                                        tCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTtggct                                               >  2:416513/1‑150 (MQ=255)
                                                                                                        tCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTtggct                                               <  1:2970985/150‑1 (MQ=255)
                                                                                                         cGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTtggctg                                              >  2:647822/1‑150 (MQ=255)
                                                                                                          gCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTCGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTtggctgg                                             >  2:3664620/1‑150 (MQ=255)
                                                                                                             aGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGgcgc                                          >  1:40696/1‑150 (MQ=255)
                                                                                                              gCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGct                                         <  1:2332990/150‑1 (MQ=255)
                                                                                                                ccGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGctct                                       >  2:4846266/1‑150 (MQ=255)
                                                                                                                     cTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGc                                  <  2:768014/150‑1 (MQ=255)
                                                                                                                      tGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGcc                                 >  2:1665157/1‑150 (MQ=255)
                                                                                                                       ggAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGccc                                >  2:4000156/1‑150 (MQ=255)
                                                                                                                        gAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGcccc                               >  1:3062911/1‑150 (MQ=255)
                                                                                                                           gCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCt                            >  1:3364326/1‑150 (MQ=255)
                                                                                                                           gCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCt                            <  1:1419495/150‑1 (MQ=255)
                                                                                                                             cAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCACTaa                          <  2:2974426/150‑1 (MQ=255)
                                                                                                                               gTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATa                        <  2:4434866/150‑1 (MQ=255)
                                                                                                                                 gATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGc                      >  2:4413250/1‑150 (MQ=255)
                                                                                                                                  atatTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCa                     >  2:3377699/1‑150 (MQ=255)
                                                                                                                                  atatTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCa                     <  2:2148155/150‑1 (MQ=255)
                                                                                                                                  atatTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCa                     <  2:2190002/150‑1 (MQ=255)
                                                                                                                                   tatTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCAt                    <  1:3145804/150‑1 (MQ=255)
                                                                                                                                    atTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATc                   <  1:4404594/150‑1 (MQ=255)
                                                                                                                                    atTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATc                   >  1:3934646/1‑150 (MQ=255)
                                                                                                                                        ccgccTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCcgcg               <  1:4276995/150‑1 (MQ=255)
                                                                                                                                          gccTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGTTGGCGCTCTACGGCCCCGCTAATAGCATCCgcggc             <  1:5035311/150‑1 (MQ=255)
                                                                                                                                          gccTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCgcggc             <  2:87860/150‑1 (MQ=255)
                                                                                                                                           ccTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCgcggcg            >  2:1034721/1‑150 (MQ=255)
                                                                                                                                             tGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCTTTGGCTGGCGCTCTACGTCCCCGCTAATAGCATCCGCGGCGtt          <  1:4752724/150‑1 (MQ=255)
                                                                                                                                              gCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTa         >  2:2795643/1‑150 (MQ=255)
                                                                                                                                               cGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAg        >  2:1766627/1‑150 (MQ=255)
                                                                                                                                                ggTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGc       >  2:4798437/1‑150 (MQ=255)
                                                                                                                                                ggTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGc       <  1:1054275/150‑1 (MQ=255)
                                                                                                                                                  tgtAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTg     >  1:3378526/1‑150 (MQ=255)
                                                                                                                                                     agtagtCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGa  >  2:187489/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGA  >  CP009273/4274492‑4274790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑