Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,274,926 A→C 100% I72M (ATT→ATG)  yjcH ← DUF485 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,274,9260AC100.0% 1175.1 / ‑6.7 299I72M (ATT→ATG) yjcHDUF485 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (164/134);  total (165/134)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 303 total aligned reads displayed.

CGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCG  >  CP009273/4274779‑4275072
                                                                                                                                                   |                                                                                                                                                  
cgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCCGATGTAGATACCGGTGAGAACAAAGGAGATCACCAt                                                                                                                                                  >  2:1153268/1‑150 (MQ=255)
cgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCAt                                                                                                                                                  >  1:4634919/1‑150 (MQ=255)
    aGCTGCGACAGGGAGTGGGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACt                                                                                                                                              <  1:2654391/150‑1 (MQ=255)
    aGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACt                                                                                                                                              >  1:5128055/1‑150 (MQ=255)
           aaaGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAacac                                                                                                                                       >  2:2273747/1‑150 (MQ=255)
           aaaGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAacac                                                                                                                                       >  2:2421736/1‑150 (MQ=255)
            aaGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACAcc                                                                                                                                      >  2:3568353/1‑150 (MQ=255)
              gggAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCaa                                                                                                                                    >  1:3500378/1‑150 (MQ=255)
              gggAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCaa                                                                                                                                    >  1:235817/1‑150 (MQ=255)
                gAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAAtt                                                                                                                                  >  1:1252656/1‑150 (MQ=255)
                gAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAAtt                                                                                                                                  <  1:1034681/150‑1 (MQ=255)
                gAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAAtt                                                                                                                                  <  2:4489379/150‑1 (MQ=255)
                   tgtgGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGa                                                                                                                               >  1:2011667/1‑150 (MQ=255)
                    gtgGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGaa                                                                                                                              >  1:2565496/1‑150 (MQ=255)
                      ggcggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATa                                                                                                                            <  2:4052148/150‑1 (MQ=255)
                       gcggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATAc                                                                                                                           >  2:2991093/1‑150 (MQ=255)
                        cggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATAcc                                                                                                                          >  1:879513/1‑150 (MQ=255)
                        cggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGGTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATAcc                                                                                                                          >  2:433555/1‑150 (MQ=255)
                         ggcAAGCGCCGTCAGAGCTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAACACCAATTGGAATACcg                                                                                                                         >  1:2247688/1‑150 (MQ=255)
                          gcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACcgc                                                                                                                        >  2:1770900/1‑150 (MQ=255)
                           cAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACcgcg                                                                                                                       >  1:4935459/1‑150 (MQ=255)
                             aGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCgtg                                                                                                                     >  1:3252211/1‑150 (MQ=255)
                               cgcCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGtgtg                                                                                                                   <  2:3697889/150‑1 (MQ=255)
                                  cGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACg                                                                                                                >  1:1070426/1‑150 (MQ=255)
                                  cGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACg                                                                                                                <  2:523356/150‑1 (MQ=255)
                                        aaCTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGgtg                                                                                                          >  1:1798710/1‑150 (MQ=255)
                                         aCTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGgtgt                                                                                                         >  2:317540/1‑150 (MQ=255)
                                           tctTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTc                                                                                                       <  1:3823761/150‑1 (MQ=255)
                                                  atgatgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCa                                                                                                >  1:3157189/1‑150 (MQ=255)
                                                     atgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAgcg                                                                                             <  2:757829/150‑1 (MQ=255)
                                                         ttGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGt                                                                                         >  2:4206120/1‑150 (MQ=255)
                                                          tGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTg                                                                                        <  2:2806350/150‑1 (MQ=255)
                                                           gTACATCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCAGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGc                                                                                       >  1:4159056/1‑150 (MQ=255)
                                                             aCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGccc                                                                                     <  1:3019070/150‑1 (MQ=255)
                                                             aCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGccc                                                                                     <  1:4017890/150‑1 (MQ=255)
                                                              ccTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCcca                                                                                    >  2:4730560/1‑150 (MQ=255)
                                                              ccTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCcca                                                                                    <  2:2604109/150‑1 (MQ=255)
                                                              ccTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGAGGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCcca                                                                                    <  2:3378526/150‑1 (MQ=255)
                                                              ccTCAGGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGAGACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCcca                                                                                    <  2:4007594/150‑1 (MQ=255)
                                                               cTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCccag                                                                                   <  1:137943/150‑1 (MQ=255)
                                                                 cATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCccagcc                                                                                 >  2:3635385/1‑150 (MQ=255)
                                                                     cAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCagccagcc                                                                             <  1:2153439/150‑1 (MQ=255)
                                                                     cAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCagccagcc                                                                             >  1:4394814/1‑150 (MQ=255)
                                                                      aGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCg                                                                            <  2:4278118/150‑1 (MQ=255)
                                                                         aCTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGAGGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGgc                                                                         <  2:4848482/150‑1 (MQ=255)
                                                                             cATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGa                                                                     <  2:1401720/150‑1 (MQ=255)
                                                                             cATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGa                                                                     >  2:864944/1‑150 (MQ=255)
                                                                             cATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGa                                                                     >  1:3195232/1‑150 (MQ=255)
                                                                              attattAAGACGGTCGAGTTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGaa                                                                    <  2:562432/150‑1 (MQ=255)
                                                                              attattAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGaa                                                                    <  1:679716/150‑1 (MQ=255)
                                                                               ttattaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAg                                                                   <  1:1840589/150‑1 (MQ=255)
                                                                                   taAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGa                                                               <  2:2472916/150‑1 (MQ=255)
                                                                                   taAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGa                                                               >  2:2189251/1‑150 (MQ=255)
                                                                                   taAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGa                                                               <  1:4420998/150‑1 (MQ=255)
                                                                                   taAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGGAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCATCACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGAGTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGa                                                               >  2:1966094/1‑150 (MQ=255)
                                                                                    aaGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGAt                                                              <  2:2509586/150‑1 (MQ=255)
                                                                                       aCGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAg                                                           <  1:150136/150‑1 (MQ=255)
                                                                                       aCGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGAGACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCACTTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAg                                                           <  2:5074933/150‑1 (MQ=255)
                                                                                        cGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGt                                                          <  1:788929/150‑1 (MQ=255)
                                                                                         ggTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTa                                                         <  2:700677/150‑1 (MQ=255)
                                                                                         ggTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTa                                                         >  2:104976/1‑150 (MQ=255)
                                                                                               aTTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCACTTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGAGCAGTAAAATaa                                                   >  2:4384914/1‑150 (MQ=255)
                                                                                               aTTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATaa                                                   >  1:3947705/1‑150 (MQ=255)
                                                                                                 tCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAg                                                 <  1:5063761/150‑1 (MQ=255)
                                                                                                 tCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAg                                                 >  2:2310245/1‑150 (MQ=255)
                                                                                                 tCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAg                                                 <  1:4096699/150‑1 (MQ=255)
                                                                                                 tCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATGGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAg                                                 <  1:1612626/150‑1 (MQ=255)
                                                                                                   gCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGcc                                               <  2:374951/150‑1 (MQ=255)
                                                                                                    ccGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCg                                              <  1:3909249/150‑1 (MQ=255)
                                                                                                    ccGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCg                                              >  1:4585592/1‑150 (MQ=255)
                                                                                                    ccGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCg                                              >  1:1993952/1‑150 (MQ=255)
                                                                                                      gTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGat                                            >  1:4112314/1‑150 (MQ=255)
                                                                                                       ttCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGata                                           <  1:3377699/150‑1 (MQ=255)
                                                                                                       ttCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGata                                           >  2:495292/1‑150 (MQ=255)
                                                                                                        tCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGatat                                          <  2:3062911/150‑1 (MQ=255)
                                                                                                           cccgccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATaaa                                       >  2:1612010/1‑150 (MQ=255)
                                                                                                           cccgccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATaaa                                       <  1:3146759/150‑1 (MQ=255)
                                                                                                             cgccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACt                                     >  1:4504071/1‑150 (MQ=255)
                                                                                                              gccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTg                                    >  1:1902388/1‑150 (MQ=255)
                                                                                                              gccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTg                                    >  1:2831287/1‑150 (MQ=255)
                                                                                                              gccAGATGTAGATACCAGTGAGAACAAAGTAGATCACCATCACTCCAACCCCAATTTGAATACCGCGTGTGACGCTTGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTg                                    >  2:1953956/1‑150 (MQ=255)
                                                                                                                 aGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCTATATAAACTGCCa                                 <  2:4477976/150‑1 (MQ=255)
                                                                                                                 aGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCa                                 >  2:3929042/1‑150 (MQ=255)
                                                                                                                  gATGTAGATACAGGTGAGAACAATGGAGATCTCCTTCACTACAACACCTATTGGATTACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCTAAGGCTATCTGTAAAATAAAGCCGTTATAAACTGcgat                                <  2:3441873/150‑4 (MQ=255)
                                                                                                                     gTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCat                             <  2:3690623/150‑1 (MQ=255)
                                                                                                                          tACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCAtaataa                        <  2:1738080/150‑1 (MQ=255)
                                                                                                                            ccGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATc                      <  2:4132955/150‑1 (MQ=255)
                                                                                                                            ccGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATc                      <  1:433555/150‑1 (MQ=255)
                                                                                                                               gTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGAc                   >  1:3575058/1‑150 (MQ=255)
                                                                                                                                tGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCACGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACa                  >  1:2066263/1‑150 (MQ=255)
                                                                                                                                  agaACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAgg                <  2:1688627/150‑1 (MQ=255)
                                                                                                                                    aaCAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAt              >  1:2475029/1‑150 (MQ=255)
                                                                                                                                     aCAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtg             >  2:1519263/1‑150 (MQ=255)
                                                                                                                                      cAAAGGAGATCACCATCACTCCAACACCAATTGGAATATCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtgg            >  2:3881231/1‑150 (MQ=255)
                                                                                                                                      cAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtgg            >  2:2455677/1‑150 (MQ=255)
                                                                                                                                       aaaGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggt           >  1:3768612/1‑150 (MQ=255)
                                                                                                                                          ggAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGc        >  1:2199370/1‑150 (MQ=255)
                                                                                                                                            agaTCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCaa      >  1:2754579/1‑150 (MQ=255)
                                                                                                                                              aTCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAAc    >  2:1242427/1‑150 (MQ=255)
                                                                                                                                                caccATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCg  >  1:1723562/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCG  >  CP009273/4274779‑4275072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑