Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,275,142 C→A 100% intergenic (‑1/+199) yjcH ← / ← acs DUF485 family inner membrane protein/acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,275,1420CA100.0% 1305.9 / ‑6.7 392intergenic (‑1/+199)yjcH/acsDUF485 family inner membrane protein/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (237/154);  total (237/155)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 402 total aligned reads displayed.

GCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTC  >  CP009273/4274995‑4275288
                                                                                                                                                   |                                                                                                                                                  
gccGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATaac                                                                                                                                                  <  2:4634919/150‑1 (MQ=255)
          aaGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCCTTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCa                                                                                                                                        >  2:2332922/1‑150 (MQ=255)
          aaGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCa                                                                                                                                        <  2:3947705/150‑1 (MQ=255)
           aGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCag                                                                                                                                       <  2:4691162/150‑1 (MQ=255)
            ggCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCaga                                                                                                                                      <  2:1508906/150‑1 (MQ=255)
             gCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCagag                                                                                                                                     >  2:2222361/1‑150 (MQ=255)
             gCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCagag                                                                                                                                     >  2:4250318/1‑150 (MQ=255)
               gATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGt                                                                                                                                   <  2:2800136/150‑1 (MQ=255)
                aTCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTa                                                                                                                                  >  2:2884373/1‑150 (MQ=255)
                 tCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTaa                                                                                                                                 <  1:4933114/150‑1 (MQ=255)
                  cAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCCCCAGAGGTAAt                                                                                                                                <  2:1902388/150‑1 (MQ=255)
                       aaaTAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAgg                                                                                                                           <  1:897649/150‑1 (MQ=255)
                       aaaTAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGGAgg                                                                                                                           >  1:3569826/1‑150 (MQ=255)
                        aaTAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAggg                                                                                                                          >  2:1262095/1‑150 (MQ=255)
                          tAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGAt                                                                                                                        >  1:4539192/1‑150 (MQ=255)
                           aaaGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGAtt                                                                                                                       >  2:2515955/1‑150 (MQ=255)
                           aaaGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGAtt                                                                                                                       >  1:4558835/1‑150 (MQ=255)
                             aGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGt                                                                                                                     >  2:3748050/1‑150 (MQ=255)
                               ccGAGATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGttt                                                                                                                   <  2:2849140/150‑1 (MQ=255)
                                cGATATAAACTGCCAGCATAATACTCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAACTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGtttt                                                                                                                  <  1:4358090/150‑1 (MQ=255)
                                cGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGtttt                                                                                                                  <  2:2183891/150‑1 (MQ=255)
                                 gATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTa                                                                                                                 >  1:1549524/1‑150 (MQ=255)
                                   tataAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAAt                                                                                                               >  1:2280387/1‑150 (MQ=255)
                                   tataAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAAt                                                                                                               >  2:3606314/1‑150 (MQ=255)
                                     taAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCTTTATTAGACTAACACCCTGAAATGCGCCTTCTCTTCTATCCGATGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAAAGTAGGGATTGTTTTAAtta                                                                                                             >  1:1768387/1‑149 (MQ=255)
                                      aaaCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTcc                                                                                                            >  1:2103984/1‑150 (MQ=255)
                                      aaaCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTcc                                                                                                            >  1:2401126/1‑150 (MQ=255)
                                      aaaCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTcc                                                                                                            <  1:3350184/150‑1 (MQ=255)
                                      aaaCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTcc                                                                                                            >  1:4265671/1‑150 (MQ=255)
                                          tGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCt                                                                                                        <  2:1993952/150‑1 (MQ=255)
                                          tGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATGGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCt                                                                                                        <  1:3881231/150‑1 (MQ=255)
                                           gCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTc                                                                                                       <  2:4148420/150‑1 (MQ=255)
                                              aGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccat                                                                                                    <  1:2973830/150‑3 (MQ=255)
                                              aGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCt                                                                                                    <  1:2501375/150‑1 (MQ=255)
                                                   aataatCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTTTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATgg                                                                                               >  2:1737625/1‑150 (MQ=255)
                                                       atCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGgaga                                                                                           <  1:1707704/150‑1 (MQ=255)
                                                         cGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAg                                                                                         >  2:4839954/1‑150 (MQ=255)
                                                             aGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTa                                                                                     >  2:2413346/1‑150 (MQ=255)
                                                             aGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTa                                                                                     >  2:2593525/1‑150 (MQ=255)
                                                             aGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTa                                                                                     >  2:26718/1‑150 (MQ=255)
                                                             aGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTa                                                                                     >  1:3194405/1‑150 (MQ=255)
                                                             aGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTGATGGGAGAAGGTTa                                                                                     >  2:3298326/1‑150 (MQ=255)
                                                               gATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAAc                                                                                   >  1:4871131/1‑150 (MQ=255)
                                                               gATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAAc                                                                                   <  1:2334619/150‑1 (MQ=255)
                                                                aTGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACg                                                                                  >  2:4793821/1‑150 (MQ=255)
                                                                   gtggCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTc                                                                               <  1:1395683/150‑1 (MQ=255)
                                                                    tggCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCg                                                                              <  2:1846763/150‑1 (MQ=255)
                                                                    tggCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCg                                                                              >  2:1610656/1‑150 (MQ=255)
                                                                    tggCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCg                                                                              <  1:4530547/150‑1 (MQ=255)
                                                                     ggCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCgg                                                                             >  1:1659800/1‑150 (MQ=255)
                                                                       cAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGt                                                                           >  2:640677/1‑150 (MQ=255)
                                                                       cAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGt                                                                           <  2:4394814/150‑1 (MQ=255)
                                                                        aaaCCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTa                                                                          >  1:3708977/1‑150 (MQ=255)
                                                                        aaaCCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTa                                                                          >  1:4095769/1‑150 (MQ=255)
                                                                        aaaCCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTa                                                                          >  1:515947/1‑150 (MQ=255)
                                                                         aaCCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTaa                                                                         <  1:833912/150‑1 (MQ=255)
                                                                            cGTTGCCTTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAAacc                                                                      >  2:1030573/1‑150 (MQ=255)
                                                                             gTTGCCGTTTTTCGACTAACTCCCTGAACTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTAGGGGAGACGGTTAACGCTGGGGTGACCCt                                                                     >  2:3165615/1‑150 (MQ=255)
                                                                             gTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCAATGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTATCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCACTTATGGGAGAAGGTTAACGCTCGGGTAACCCt                                                                     >  1:4063488/1‑150 (MQ=255)
                                                                              ttGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCtt                                                                    <  2:5049924/150‑1 (MQ=255)
                                                                                 ccGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGcc                                                                 <  1:2455677/150‑1 (MQ=255)
                                                                                  cGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCg                                                                >  1:2594792/1‑150 (MQ=255)
                                                                                   gTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATACATAACAGATTCTCCAGAGGTACTGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGa                                                               >  2:3130831/1‑150 (MQ=255)
                                                                                    tttttCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGaa                                                              >  2:810972/1‑150 (MQ=255)
                                                                                     ttttCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAAt                                                             <  1:3294839/150‑1 (MQ=255)
                                                                                       ttCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGt                                                           >  1:5075913/1‑150 (MQ=255)
                                                                                        tCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTa                                                          >  1:1314527/1‑150 (MQ=255)
                                                                                         cGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAg                                                         >  1:189922/1‑150 (MQ=255)
                                                                                         cGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAg                                                         >  1:1496281/1‑150 (MQ=255)
                                                                                           aCTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTTGGc                                                       >  2:1718918/1‑150 (MQ=255)
                                                                                            cTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGcc                                                      <  2:3819691/150‑1 (MQ=255)
                                                                                             tAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCg                                                     >  1:1215995/1‑150 (MQ=255)
                                                                                                 tCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAAAGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATa                                                 >  1:1154754/1‑150 (MQ=255)
                                                                                                    cTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAgg                                              <  2:2066263/150‑1 (MQ=255)
                                                                                                    cTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAgg                                              >  1:3042148/1‑150 (MQ=255)
                                                                                                        aaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGtt                                          >  1:4158503/1‑150 (MQ=255)
                                                                                                            cgcATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTAcg                                      >  2:3215632/1‑150 (MQ=255)
                                                                                                               aTTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTAcgccg                                   >  1:4637285/1‑150 (MQ=255)
                                                                                                                ttGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTAcgccgc                                  <  1:2801963/150‑1 (MQ=255)
                                                                                                                       ctATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                           >  2:5163251/1‑150 (MQ=255)
                                                                                                                        tATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGca                          >  1:4195943/1‑150 (MQ=255)
                                                                                                                        tATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGca                          >  2:1220942/1‑150 (MQ=255)
                                                                                                                           ccGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGcaatc                       >  2:4072098/1‑150 (MQ=255)
                                                                                                                           ccGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGcaatc                       >  1:1107178/1‑150 (MQ=255)
                                                                                                                            cGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGcaatca                      <  1:662927/150‑1 (MQ=255)
                                                                                                                                 aTAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGcc                 >  1:1778198/1‑150 (MQ=255)
                                                                                                                                 aTAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGcc                 >  2:136709/1‑150 (MQ=255)
                                                                                                                                  tAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAATCAATGCCt                <  2:71114/150‑1 (MQ=255)
                                                                                                                                   aaaTAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTg               >  1:2721253/1‑150 (MQ=255)
                                                                                                                                    aaTAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGa              >  2:4535255/1‑150 (MQ=255)
                                                                                                                                      tAGTGCCATTCATAACAGATTCTCCAGAGGTACTGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATg            <  2:341129/150‑1 (MQ=255)
                                                                                                                                      tAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATg            <  2:926459/150‑1 (MQ=255)
                                                                                                                                      tAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATg            >  1:444947/1‑150 (MQ=255)
                                                                                                                                      tAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATg            >  2:4625643/1‑150 (MQ=255)
                                                                                                                                         tGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGa         >  2:1287225/1‑150 (MQ=255)
                                                                                                                                         tGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGa         >  2:4066098/1‑150 (MQ=255)
                                                                                                                                          gCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGAc        <  2:1683182/150‑1 (MQ=255)
                                                                                                                                          gCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGAc        <  2:1808706/150‑1 (MQ=255)
                                                                                                                                           ccATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACg       >  1:3844925/1‑150 (MQ=255)
                                                                                                                                              ttCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTg    <  1:3480703/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTC  >  CP009273/4274995‑4275288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑