Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,288,086 G→T 100% F366L (TTC→TTA)  fdhF ← formate dehydrogenase‑H, selenopolypeptide subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,288,0860GT98.6% 1128.9 / 3.3 346F366L (TTC→TTA) fdhFformate dehydrogenase‑H, selenopolypeptide subunit
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base T (166/175);  total (169/177)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 346 total aligned reads displayed.

TACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCA  >  CP009273/4287939‑4288231
                                                                                                                                                   |                                                                                                                                                 
tACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTaa                                                                                                                                                 >  2:3159821/1‑150 (MQ=255)
  cTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAAtt                                                                                                                                               >  2:4596035/1‑150 (MQ=255)
  cTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAAtt                                                                                                                                               >  2:3807030/1‑150 (MQ=255)
   tGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAAttt                                                                                                                                              >  1:4482743/1‑150 (MQ=255)
   tGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAAttt                                                                                                                                              <  2:2705357/150‑1 (MQ=255)
     ccGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTtct                                                                                                                                            >  1:4279558/1‑150 (MQ=255)
     ccGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTtct                                                                                                                                            <  1:673867/150‑1 (MQ=255)
      cGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTtctc                                                                                                                                           >  1:206260/1‑150 (MQ=255)
       gACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTcg                                                                                                                                          <  2:4115177/150‑1 (MQ=255)
       gACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTcg                                                                                                                                          >  1:983427/1‑150 (MQ=255)
        aCAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTcgc                                                                                                                                         >  1:3293124/1‑150 (MQ=255)
         cAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTcgcg                                                                                                                                        >  1:3639431/1‑150 (MQ=255)
         cAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTcgcg                                                                                                                                        >  2:2520691/1‑150 (MQ=255)
          aGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTAGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATATCCGGTATGCGCTGTCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCgg                                                                                                                                       >  1:1029813/1‑150 (MQ=255)
            cTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGtt                                                                                                                                     >  1:1111618/1‑150 (MQ=255)
             tCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTa                                                                                                                                    <  2:4374763/150‑1 (MQ=255)
              ccGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAg                                                                                                                                   <  1:3847869/150‑1 (MQ=255)
               cgcgTCAGTTTGTAGCGGATCTTCGCCCCTAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTCGc                                                                                                                                  >  2:1321488/1‑150 (MQ=255)
                       tttGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCtt                                                                                                                          <  1:1932979/150‑1 (MQ=255)
                          gTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCa                                                                                                                       >  1:2197682/1‑150 (MQ=255)
                           tAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCAc                                                                                                                      >  2:3620628/1‑150 (MQ=255)
                           tAGCGGATCGTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCGCGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCAc                                                                                                                      <  2:4438926/150‑1 (MQ=255)
                            aGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACg                                                                                                                     <  1:2600757/150‑1 (MQ=255)
                                ggtCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACt                                                                                                                 >  1:1671374/3‑150 (MQ=255)
                                 aTCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTg                                                                                                                >  2:4336639/1‑150 (MQ=255)
                                  tCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTgg                                                                                                               >  2:2578379/1‑150 (MQ=255)
                                   cTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGt                                                                                                              <  1:2989296/150‑1 (MQ=255)
                                      cGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATc                                                                                                           <  1:3625807/150‑1 (MQ=255)
                                       gCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATcc                                                                                                          >  2:1047113/1‑150 (MQ=255)
                                       gCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATcc                                                                                                          >  1:3449501/1‑150 (MQ=255)
                                             aaTGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATa                                                                                                    <  2:742611/150‑1 (MQ=255)
                                                 tACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTa                                                                                                >  1:4725496/1‑150 (MQ=255)
                                                  aCGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTAt                                                                                               <  2:1110896/150‑1 (MQ=255)
                                                   cgcgGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATc                                                                                              <  1:436895/150‑1 (MQ=255)
                                                    gcgGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATcc                                                                                             <  1:3253752/150‑1 (MQ=255)
                                                     cgGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCg                                                                                            >  2:3846012/1‑150 (MQ=255)
                                                     cgGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCg                                                                                            >  1:538898/1‑150 (MQ=255)
                                                      ggCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCgg                                                                                           >  2:3505669/1‑150 (MQ=255)
                                                                 cGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCa                                                                                >  2:4623677/1‑150 (MQ=255)
                                                                 cGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCa                                                                                >  1:3762893/1‑150 (MQ=255)
                                                                  gCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCat                                                                               >  2:898392/1‑150 (MQ=255)
                                                                  gCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCat                                                                               <  1:2924683/150‑1 (MQ=255)
                                                                   ccATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCata                                                                              <  1:4989329/150‑1 (MQ=255)
                                                                     aTGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATc                                                                            <  1:2530661/150‑1 (MQ=255)
                                                                     aTGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATc                                                                            >  1:4885089/1‑150 (MQ=255)
                                                                        cgctgcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCa                                                                         >  1:2774318/1‑150 (MQ=255)
                                                                          ctgcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAgg                                                                       <  1:4546867/150‑1 (MQ=255)
                                                                          ctgcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAgg                                                                       >  2:3150704/1‑150 (MQ=255)
                                                                            gcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCa                                                                     <  1:1192806/150‑1 (MQ=255)
                                                                                ggTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCt                                                                 <  1:4849322/150‑1 (MQ=255)
                                                                                ggTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCt                                                                 >  2:652066/1‑150 (MQ=255)
                                                                                ggTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCt                                                                 >  2:611001/1‑150 (MQ=255)
                                                                                ggTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCt                                                                 >  2:2592197/1‑150 (MQ=255)
                                                                                  tGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGa                                                               >  2:805603/1‑150 (MQ=255)
                                                                                   gCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGaa                                                              <  1:4974077/150‑1 (MQ=255)
                                                                                        aGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACgttg                                                         <  1:2020765/150‑1 (MQ=255)
                                                                                         gCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACgttgt                                                        <  2:2392733/150‑1 (MQ=255)
                                                                                          cTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACgttgtt                                                       <  2:3306551/150‑1 (MQ=255)
                                                                                            cGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCt                                                     <  1:2331020/150‑1 (MQ=255)
                                                                                            cGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCt                                                     <  2:493628/150‑1 (MQ=255)
                                                                                            cGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCt                                                     >  1:4186505/1‑150 (MQ=255)
                                                                                            cGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATGTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCt                                                     <  2:2130757/150‑1 (MQ=255)
                                                                                              cTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGa                                                   >  2:421707/1‑150 (MQ=255)
                                                                                               tGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGAc                                                  <  2:4279558/150‑1 (MQ=255)
                                                                                                 aTGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCa                                                <  2:4856273/150‑1 (MQ=255)
                                                                                                  tGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCAc                                               <  1:4606458/150‑1 (MQ=255)
                                                                                                  tGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCAc                                               <  1:2502086/150‑1 (MQ=255)
                                                                                                      aTAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAAc                                           <  1:3244547/150‑1 (MQ=255)
                                                                                                       tAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAAcc                                          <  2:5086351/150‑1 (MQ=255)
                                                                                                         gCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCgg                                        >  1:2790633/1‑150 (MQ=255)
                                                                                                          ccGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCggg                                       >  1:1638900/1‑150 (MQ=255)
                                                                                                             gTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTa                                    <  2:2906372/150‑1 (MQ=255)
                                                                                                               aTGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAac                                  <  2:4482743/150‑1 (MQ=255)
                                                                                                                tGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAaca                                 <  2:2060715/150‑1 (MQ=255)
                                                                                                                    cTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCg                             <  2:1230135/150‑1 (MQ=255)
                                                                                                                     tGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCCGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGc                            >  1:1914797/1‑150 (MQ=255)
                                                                                                                      ggCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCa                           >  2:3234842/1‑150 (MQ=255)
                                                                                                                        cAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATg                         >  1:3129648/1‑150 (MQ=255)
                                                                                                                        cAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATg                         <  1:1753132/150‑1 (MQ=255)
                                                                                                                        cAGGCTTTACACTCCCCAGGCTTTGGCTAATTTCTCCCGGATAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGAGACCATATCGCAGGCACCCTGAACTTTGTTCTGACCACTAACCGGGTTAACTCCCGCATg                         <  2:3028055/150‑1 (MQ=255)
                                                                                                                         aGGCTTTCTACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGc                        <  2:1840602/150‑1 (MQ=255)
                                                                                                                         aGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGACGGATCTTTCACGTACTGCTATCCCGGATACGTATCCGGCAGCGAGCCCATATAGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGc                        >  1:4572925/1‑150 (MQ=255)
                                                                                                                          ggCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCg                       <  2:2807078/150‑1 (MQ=255)
                                                                                                                          ggCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCg                       <  2:2200069/150‑1 (MQ=255)
                                                                                                                          ggCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGGATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCg                       <  2:1572760/150‑1 (MQ=255)
                                                                                                                               tCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTa                  <  1:4155905/150‑1 (MQ=255)
                                                                                                                                 cACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTAcc                <  2:1965833/150‑1 (MQ=255)
                                                                                                                                 cACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTAcc                <  2:4958838/150‑1 (MQ=255)
                                                                                                                                 cACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCAGATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTAcc                <  2:5158937/150‑1 (MQ=255)
                                                                                                                                 cACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTAcc                <  2:1834218/150‑1 (MQ=255)
                                                                                                                                   cGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGa              <  2:3639431/150‑1 (MQ=255)
                                                                                                                                   cGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGa              <  1:1539936/150‑1 (MQ=255)
                                                                                                                                       ccAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGtt          >  1:793341/1‑150 (MQ=255)
                                                                                                                                        cAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTa         <  1:708108/150‑1 (MQ=255)
                                                                                                                                          ggCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTAcc       <  2:4023653/150‑1 (MQ=255)
                                                                                                                                           gCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCg      >  2:2491868/1‑150 (MQ=255)
                                                                                                                                            cTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCgg     <  2:819578/150‑1 (MQ=255)
                                                                                                                                            cTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCACATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTTCCGAGGTTACCgg     <  2:1692417/150‑1 (MQ=255)
                                                                                                                                               tGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCa  >  1:3975229/1‑150 (MQ=255)
                                                                                                                                               tGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCAGGATACGTATCCTGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCa  >  1:4213864/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCA  >  CP009273/4287939‑4288231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑