Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,297,846 C→A 100% M150I (ATG→ATT)  alsE ← allulose‑6‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,297,8460CA100.0% 1203.2 / NA 355M150I (ATG→ATT) alsEallulose‑6‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (189/166);  total (189/166)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 355 total aligned reads displayed.

CAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGAT  >  CP009273/4297699‑4297992
                                                                                                                                                   |                                                                                                                                                  
caagCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAAt                                                                                                                                                  <  1:3399573/147‑1 (MQ=255)
 aGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAAtt                                                                                                                                                 >  1:4304850/1‑150 (MQ=255)
  ggCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAAttt                                                                                                                                                <  1:2038355/150‑1 (MQ=255)
    ccGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCa                                                                                                                                              >  2:2609679/1‑150 (MQ=255)
      ggAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAgg                                                                                                                                            >  1:1052301/1‑150 (MQ=255)
        aaGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGaa                                                                                                                                          >  2:2356785/1‑150 (MQ=255)
        aaGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGaa                                                                                                                                          >  2:3390056/1‑150 (MQ=255)
         aGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAAt                                                                                                                                         >  2:4466353/1‑150 (MQ=255)
            gCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGaa                                                                                                                                      <  1:4812580/150‑1 (MQ=255)
             ccGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAAc                                                                                                                                     >  1:356152/1‑150 (MQ=255)
              cgacgaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACg                                                                                                                                    >  2:2168065/1‑150 (MQ=255)
                 cgaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGtt                                                                                                                                 >  2:1356823/1‑150 (MQ=255)
                  gaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTg                                                                                                                                >  2:557561/1‑150 (MQ=255)
                  gaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTg                                                                                                                                >  1:3665837/1‑150 (MQ=255)
                  gaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAAGTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTg                                                                                                                                <  2:4656243/150‑1 (MQ=255)
                   aTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGt                                                                                                                               <  2:2977170/150‑1 (MQ=255)
                   aTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGt                                                                                                                               >  1:1349232/1‑150 (MQ=255)
                     aaaGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTcc                                                                                                                             >  1:4228187/1‑150 (MQ=255)
                      aaGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCg                                                                                                                            >  1:3770680/1‑150 (MQ=255)
                      aaGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCg                                                                                                                            <  1:3387034/150‑1 (MQ=255)
                       aGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCgg                                                                                                                           >  2:5045675/1‑150 (MQ=255)
                           aTCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCaaa                                                                                                                       >  2:2461863/1‑150 (MQ=255)
                           aTCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCaaa                                                                                                                       >  1:544725/1‑150 (MQ=255)
                            tCCGCCCCTGCCGCCATCAGTTTTTCGTAAGGTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAg                                                                                                                      <  2:2296069/150‑1 (MQ=255)
                              cGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGcc                                                                                                                    >  1:520830/1‑150 (MQ=255)
                               gCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCg                                                                                                                   >  2:3909771/1‑150 (MQ=255)
                                ccccTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCgg                                                                                                                  >  1:1602457/1‑150 (MQ=255)
                                      ccgccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGa                                                                                                            <  2:1325399/150‑1 (MQ=255)
                                       cgccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGAc                                                                                                           >  1:2238905/1‑150 (MQ=255)
                                       cgccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGAc                                                                                                           <  2:4922847/150‑1 (MQ=255)
                                       cgccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGAc                                                                                                           <  2:4373554/150‑1 (MQ=255)
                                        gccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACa                                                                                                          >  2:4106035/1‑150 (MQ=255)
                                          cATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGt                                                                                                        <  2:4339257/150‑1 (MQ=255)
                                          cATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCCCGTTCACGCCATGCATTCAGTTCGGCAAGTTTATCCATAATTTCAGGAATGAACGGTTGTCCGGAAAAGCCGGGATCGACAGt                                                                                                        <  2:1877642/150‑1 (MQ=255)
                                           aTCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTc                                                                                                       >  2:4155393/1‑150 (MQ=255)
                                           aTCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTc                                                                                                       >  1:238312/1‑150 (MQ=255)
                                           aTCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTc                                                                                                       <  2:2801834/150‑1 (MQ=255)
                                            tCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCa                                                                                                      <  2:4571973/150‑1 (MQ=255)
                                            tCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCa                                                                                                      >  1:4716707/1‑150 (MQ=255)
                                              aGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATg                                                                                                    >  1:5116304/1‑150 (MQ=255)
                                                 ttttCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCTAAGCCGGGATCGACAGTCATGAcc                                                                                                 <  2:4285551/150‑1 (MQ=255)
                                                   ttCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGt                                                                                               >  1:3319525/1‑150 (MQ=255)
                                                   ttCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGt                                                                                               >  1:2460302/1‑150 (MQ=255)
                                                     cGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTaa                                                                                             >  2:1027915/1‑150 (MQ=255)
                                                      gTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAt                                                                                            >  1:449950/1‑150 (MQ=255)
                                                       tAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAtt                                                                                           <  1:888688/150‑1 (MQ=255)
                                                       tAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAtt                                                                                           >  1:3855272/1‑150 (MQ=255)
                                                        aaGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAttt                                                                                          <  2:1121688/150‑1 (MQ=255)
                                                         aGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAtttt                                                                                         <  2:144790/150‑1 (MQ=255)
                                                          gTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTa                                                                                        >  1:2358136/1‑150 (MQ=255)
                                                           ttGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTAt                                                                                       >  2:3562367/1‑150 (MQ=255)
                                                            tGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATc                                                                                      >  2:1178382/1‑150 (MQ=255)
                                                                     gCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATg                                                                             <  1:2181736/150‑1 (MQ=255)
                                                                       aGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGa                                                                           >  2:3491718/1‑150 (MQ=255)
                                                                         gAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGata                                                                         <  2:2200121/150‑1 (MQ=255)
                                                                             cGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGt                                                                     >  1:2205638/1‑150 (MQ=255)
                                                                             cGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGt                                                                     >  1:176857/1‑150 (MQ=255)
                                                                              gTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTa                                                                    >  1:1554788/1‑150 (MQ=255)
                                                                              gTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTa                                                                    >  2:2937021/1‑150 (MQ=255)
                                                                               tCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTAt                                                                   <  2:304703/150‑1 (MQ=255)
                                                                                 cACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTAttt                                                                 <  1:3359105/150‑1 (MQ=255)
                                                                                      cAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATgg                                                            >  1:1817856/1‑150 (MQ=255)
                                                                                       aaTTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGc                                                           <  1:3100260/150‑1 (MQ=255)
                                                                                       aaTTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGc                                                           >  1:2198370/1‑150 (MQ=255)
                                                                                       aaTTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGACAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGc                                                           >  1:148219/1‑150 (MQ=255)
                                                                                        aTTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGcc                                                          <  2:3666152/150‑1 (MQ=255)
                                                                                           tCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCa                                                       <  2:3077543/150‑1 (MQ=255)
                                                                                           tCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCa                                                       <  1:109813/150‑1 (MQ=255)
                                                                                           tCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCa                                                       <  2:1967547/150‑1 (MQ=255)
                                                                                           tCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCa                                                       >  1:3015762/1‑150 (MQ=255)
                                                                                            cGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCaa                                                      <  1:411823/150‑1 (MQ=255)
                                                                                              tACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACt                                                    <  2:2557327/150‑1 (MQ=255)
                                                                                              tACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACt                                                    >  1:3146297/1‑150 (MQ=255)
                                                                                               aCTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTg                                                   <  2:3973422/150‑1 (MQ=255)
                                                                                                 tCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCAGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGc                                                 >  2:1557607/1‑150 (MQ=255)
                                                                                                   cAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGt                                               >  2:629501/1‑150 (MQ=255)
                                                                                                       ccTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTcc                                           <  1:646964/150‑1 (MQ=255)
                                                                                                             cGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTa                                     <  2:3205932/150‑1 (MQ=255)
                                                                                                              gTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTaa                                    <  2:3904192/150‑1 (MQ=255)
                                                                                                              gTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCCCAACTGGCGTCTCCGGGTTaa                                    <  2:2875268/150‑1 (MQ=255)
                                                                                                               ttCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAg                                   <  1:2972250/150‑1 (MQ=255)
                                                                                                                tCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAgg                                  <  1:3962225/150‑1 (MQ=255)
                                                                                                                  aCGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGAt                                <  2:901021/150‑1 (MQ=255)
                                                                                                                       aTGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGcc                           >  1:4752686/1‑150 (MQ=255)
                                                                                                                       aTGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGcc                           >  2:1583518/1‑150 (MQ=255)
                                                                                                                        tGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGccc                          >  2:3582656/1‑150 (MQ=255)
                                                                                                                         gCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGcccc                         >  1:4983533/1‑150 (MQ=255)
                                                                                                                           cTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCAc                       <  1:5031764/150‑1 (MQ=255)
                                                                                                                            ttCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACt                      >  2:1213867/1‑150 (MQ=255)
                                                                                                                            ttCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACt                      >  1:243117/1‑150 (MQ=255)
                                                                                                                              cAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCGTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACttt                    >  1:1325088/1‑150 (MQ=255)
                                                                                                                                gTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtca                  <  1:2852072/150‑1 (MQ=255)
                                                                                                                                gTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtca                  <  2:32491/150‑1 (MQ=255)
                                                                                                                                      cAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtcat            <  2:1633621/150‑1 (MQ=255)
                                                                                                                                      cAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtcat            >  2:1770551/1‑150 (MQ=255)
                                                                                                                                        aGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGa          >  1:1938404/1‑150 (MQ=255)
                                                                                                                                             aTCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGAcggcg     <  1:3506449/150‑1 (MQ=255)
                                                                                                                                             aTCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGAcggcg     >  2:2052375/1‑150 (MQ=255)
                                                                                                                                              tCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGAcggcgg    <  1:2755680/150‑1 (MQ=255)
                                                                                                                                              tCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGAcggcgg    <  1:2718264/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGAT  >  CP009273/4297699‑4297992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑