Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,329,774 C→A 100% D189Y (GAC→TAC)  adiA ← arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,329,7740CA98.3% 891.1 / 4.5 286D189Y (GAC→TAC) adiAarginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base A (144/134);  total (147/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 286 total aligned reads displayed.

TGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCG  >  CP009273/4329627‑4329920
                                                                                                                                                   |                                                                                                                                                  
tgatgGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAgg                                                                                                                                                  >  2:226337/1‑150 (MQ=255)
  atgGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTg                                                                                                                                                >  2:9642/1‑150 (MQ=255)
  atgGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTg                                                                                                                                                <  2:3754323/150‑1 (MQ=255)
     gTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCgg                                                                                                                                             <  2:1778833/150‑1 (MQ=255)
      tGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGa                                                                                                                                            <  2:4206131/150‑1 (MQ=255)
       gCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGaa                                                                                                                                           <  2:3386755/150‑1 (MQ=255)
           ttAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGa                                                                                                                                       >  1:1280119/1‑150 (MQ=255)
           taagagCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCAAAATGCGACAGTATGGTCAAGCAAAGAACCGAGTGAAGTTCGTTCGATGACCATGTAGGTGCGGAACAGa                                                                                                                                       >  2:699219/3‑150 (MQ=255)
            tAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGAt                                                                                                                                      >  2:395309/1‑150 (MQ=255)
             agagCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCATAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGAtt                                                                                                                                     <  1:4888338/150‑1 (MQ=255)
             agagCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGAtt                                                                                                                                     >  1:2417014/1‑150 (MQ=255)
                    gAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCa                                                                                                                              <  2:258202/150‑1 (MQ=255)
                     aaGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCAt                                                                                                                             <  1:2575985/150‑1 (MQ=255)
                          ccGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCAtagtag                                                                                                                        <  1:2822703/150‑1 (MQ=255)
                            gactacTACCGACCACGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTc                                                                                                                      <  2:2647351/150‑1 (MQ=255)
                             acgacTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCa                                                                                                                     <  1:3573363/150‑1 (MQ=255)
                               gacTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATg                                                                                                                   >  2:3509356/1‑150 (MQ=255)
                                 cTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGt                                                                                                                 <  1:3938353/150‑1 (MQ=255)
                                  tACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTa                                                                                                                >  1:4021969/1‑150 (MQ=255)
                                  tACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTa                                                                                                                >  1:1902731/1‑150 (MQ=255)
                                  tACCGACCAGGAGCGATCGGCACCAAAGACGCGTGCGGCATATTTTTCGCTTTCGCAAAATGCGCCAGTTTGGTCAAGCAAAGAAGCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTa                                                                                                                <  1:4366205/150‑1 (MQ=255)
                                   aCCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAg                                                                                                               <  2:4338763/150‑1 (MQ=255)
                                     cGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGACGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGaa                                                                                                             >  2:201852/1‑150 (MQ=255)
                                     cGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGaa                                                                                                             <  1:2955200/150‑1 (MQ=255)
                                       aCCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAAc                                                                                                           <  2:4388053/150‑1 (MQ=255)
                                           ggAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTcc                                                                                                       >  1:1747472/1‑150 (MQ=255)
                                             aGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTCGTCATGGTAGAAACGTCcgg                                                                                                     >  2:343225/1‑150 (MQ=255)
                                             aGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcgg                                                                                                     >  2:4694571/1‑150 (MQ=255)
                                             aGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcgg                                                                                                     >  2:2125712/1‑150 (MQ=255)
                                               tgATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcggcg                                                                                                   >  2:2660547/2‑150 (MQ=255)
                                                gATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcggcgg                                                                                                  >  1:2611973/1‑150 (MQ=255)
                                                gATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcggcgg                                                                                                  >  1:2211802/1‑150 (MQ=255)
                                                      cACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTCGAAACGTCCGGCGGGTGttt                                                                                            >  2:1186861/1‑150 (MQ=255)
                                                      cACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGttt                                                                                            >  1:4561616/1‑150 (MQ=255)
                                                      cACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGttt                                                                                            <  2:1676188/150‑1 (MQ=255)
                                                       aCCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGtttt                                                                                           >  1:3810817/1‑150 (MQ=255)
                                                         cAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTgg                                                                                         >  2:2758076/1‑150 (MQ=255)
                                                          aaaTACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGt                                                                                        >  2:3009748/1‑150 (MQ=255)
                                                          aaaTACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGt                                                                                        >  1:2120345/1‑150 (MQ=255)
                                                           aaTACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTa                                                                                       <  2:1506812/150‑1 (MQ=255)
                                                           aaTACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTa                                                                                       <  2:282136/150‑1 (MQ=255)
                                                                  gTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCaa                                                                                >  1:3805383/1‑150 (MQ=255)
                                                                   tGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCCTGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAAc                                                                               >  1:3374253/1‑150 (MQ=255)
                                                                   tGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAAc                                                                               <  1:915623/150‑1 (MQ=255)
                                                                   tGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAAc                                                                               <  2:1561571/150‑1 (MQ=255)
                                                                      ggCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAAcgcc                                                                            <  1:664553/150‑1 (MQ=255)
                                                                         atatTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACgccgcc                                                                         >  2:21518/1‑150 (MQ=255)
                                                                         atatTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACgccgcc                                                                         >  1:1613923/1‑150 (MQ=255)
                                                                         atatTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACgccgcc                                                                         >  2:1353583/1‑150 (MQ=255)
                                                                          tatTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGccc                                                                        >  1:4697051/1‑150 (MQ=255)
                                                                              tttcgctttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCtggt                                                                    <  1:1398148/150‑1 (MQ=255)
                                                                               ttcgctttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCtggtg                                                                   >  1:4104704/1‑150 (MQ=255)
                                                                               ttcgctttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCtggtg                                                                   <  1:854001/150‑1 (MQ=255)
                                                                                  gctttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGATGTTCGTTCGATGCCCATGTTGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGcc                                                                <  1:885712/150‑1 (MQ=255)
                                                                                  gctttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGACGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGcc                                                                >  1:3632936/1‑150 (MQ=255)
                                                                                  gctttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTAACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGcc                                                                >  2:1079591/1‑150 (MQ=255)
                                                                                   ctttcgcCAATTGGGCCAGTTTGGTCAAGCAATGAACGGTGGGTTGTTCTTTCGATGCACTTTTTGGTGCTGAACTTATTTTCACCATTTTATTCATGGTTTAAACTTCCGGGGGGTGTTTTGTTAAAACCAACGCCGCCCTGGTGGCCt                                                               <  1:1334591/150‑1 (MQ=255)
                                                                                   ctttcgcCAATTGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTTGGTGCGTAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCt                                                               <  1:4926035/150‑1 (MQ=255)
                                                                                    tttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTg                                                              <  1:251549/150‑1 (MQ=255)
                                                                                      tcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGgc                                                            <  1:2396860/150‑1 (MQ=255)
                                                                                      tcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGgc                                                            >  2:1515746/1‑150 (MQ=255)
                                                                                      tcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGgc                                                            <  1:179249/150‑1 (MQ=255)
                                                                                      tcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGgc                                                            >  1:4816479/1‑150 (MQ=255)
                                                                                       cgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGgcg                                                           >  2:2926361/1‑150 (MQ=255)
                                                                                       cgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGgcg                                                           >  1:3840096/1‑150 (MQ=255)
                                                                                        gcCACAGGCGCCAGTATGGTCAAGCAAAGCACCGCGGGAAGTTCGTTCGAGGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGgcgc                                                          <  1:2292059/150‑1 (MQ=255)
                                                                                         ccAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCt                                                         <  1:105823/150‑1 (MQ=255)
                                                                                          cAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTg                                                        <  1:106037/150‑1 (MQ=255)
                                                                                           aaaTGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGc                                                       >  1:1191879/1‑150 (MQ=255)
                                                                                               gcgcCAGTATGGTCAAGCAAAGAACCTAGGGAAGATCGTTCGAAGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAAAGTCCGGCGGGTGTTTTGGTAAAACCAAAGCCGCCCTGGTGGCCTGTCGCTGCCCAg                                                   >  1:4134384/1‑150 (MQ=255)
                                                                                               gcgcCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAg                                                   <  2:4917896/150‑1 (MQ=255)
                                                                                                cgcCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAgg                                                  <  1:766528/150‑1 (MQ=255)
                                                                                                  ccAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCCGGac                                                >  2:2023784/1‑149 (MQ=255)
                                                                                                   cAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAat                                               <  2:2421398/150‑1 (MQ=255)
                                                                                                    aGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAata                                              <  2:4566582/150‑1 (MQ=255)
                                                                                                       aTGGTCAAGCAAAGAACCGAGGGACGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCAGGGTAGAAACGTCCGGCGGGTGGTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTc                                           >  2:4482136/1‑150 (MQ=255)
                                                                                                        tGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCa                                          <  1:2704743/150‑1 (MQ=255)
                                                                                                           tCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATgg                                       <  1:4331585/150‑1 (MQ=255)
                                                                                                           tCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATgg                                       >  1:1533620/1‑150 (MQ=255)
                                                                                                            cAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCCGGAATATTCATGGa                                      >  2:2293253/1‑150 (MQ=255)
                                                                                                              aGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATg                                    >  2:821336/1‑150 (MQ=255)
                                                                                                                cAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTc                                  <  2:3792547/150‑1 (MQ=255)
                                                                                                                 aaaGAACCGAGGGAAATTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCAGGCGGGTGTTTTGGTAAAACCAACTCCGACCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCa                                 >  2:3070445/1‑150 (MQ=255)
                                                                                                                  aaGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCAc                                <  2:2076989/150‑1 (MQ=255)
                                                                                                                   aGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACt                               >  2:1316849/1‑150 (MQ=255)
                                                                                                                   aGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACt                               <  1:3201385/150‑1 (MQ=255)
                                                                                                                       ccGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTatat                           >  1:3008911/1‑150 (MQ=255)
                                                                                                                       ccGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTatat                           >  2:1268625/1‑150 (MQ=255)
                                                                                                                        cGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATAtt                          <  2:163473/150‑1 (MQ=255)
                                                                                                                           gggAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTtca                       >  2:3184464/1‑150 (MQ=255)
                                                                                                                            ggAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTtcat                      >  1:149028/1‑150 (MQ=255)
                                                                                                                               tgttcgttcGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAg                   <  1:3666924/149‑1 (MQ=255)
                                                                                                                                gttcgttcGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAgc                  <  1:4424992/150‑1 (MQ=255)
                                                                                                                                 ttcgttcgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAgcg                 >  2:5148099/1‑150 (MQ=255)
                                                                                                                                 ttcgttcgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAgcg                 >  1:1119847/1‑150 (MQ=255)
                                                                                                                                  tcgttcgATGCCCCTGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAgcgc                <  2:304041/150‑1 (MQ=255)
                                                                                                                                  tcgttcgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAgcgc                >  2:3686232/1‑150 (MQ=255)
                                                                                                                                        gATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGaa          <  2:3579601/150‑1 (MQ=255)
                                                                                                                                              cATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGACCAGTgg    >  2:198669/1‑150 (MQ=255)
                                                                                                                                              cATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTgg    >  2:943845/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCG  >  CP009273/4329627‑4329920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑