BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,359,686	C→A	51.3%	E182* (GAA→TAA)	yjeH ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,359,686	0	C	A	51.3%	75.2 / inf	278	E182* (GAA→TAA)	yjeH	putative transporter
Reads supporting (aligned to +/- strand): ref base C (83/52); new base A (66/76); total (149/129)
Fisher's exact test for biased strand distribution p-value = 1.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01

Only 100 of 279 total aligned reads displayed.

ACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGATATACCACACCA  >  CP009273/4359538‑4359835

  tAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGat                                                                                                                                                    <  1:4222164/150‑1 (MQ=255)

    aTCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGatat                                                                                                                                                  <  1:3750692/150‑1 (MQ=255)

    aTCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGatat                                                                                                                                                  <  2:3069857/150‑1 (MQ=255)

    aTCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGatat                                                                                                                                                  <  1:2227602/150‑1 (MQ=255)

      ccTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTa                                                                                                                                                >  1:1868997/1‑150 (MQ=255)

        tGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTAcc                                                                                                                                              <  1:1003945/150‑1 (MQ=255)

         gCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCa                                                                                                                                             >  1:516094/1‑150 (MQ=255)

            agcagcagACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGt                                                                                                                                          >  1:968020/1‑150 (MQ=255)

                gcagACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCg                                                                                                                                      >  1:3993619/1‑150 (MQ=255)

                 cagACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCgg                                                                                                                                     >  2:4569097/1‑150 (MQ=255)

                   gACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGaa                                                                                                                                   <  1:707175/150‑1 (MQ=255)

                     ccAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAg                                                                                                                                 <  2:3824178/150‑1 (MQ=255)

                         tcatcaAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGa                                                                                                                             <  2:1869594/150‑1 (MQ=255)

                         tcatcaAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGa                                                                                                                             <  1:2838731/150‑1 (MQ=255)

                            tcaAATCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGatat                                                                                                                          <  1:3501717/150‑1 (MQ=255)

                            tcaAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGatat                                                                                                                          >  1:136569/1‑150 (MQ=255)

                              aaaGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTc                                                                                                                        <  2:3586429/150‑1 (MQ=255)

                               aaGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCg                                                                                                                       <  2:4423525/150‑1 (MQ=255)

                                 gCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCa                                                                                                                     >  2:2696910/1‑150 (MQ=255)

                                   aCGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCATCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAgg                                                                                                                   >  1:4594234/1‑150 (MQ=255)

                                       ggAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGGAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTg                                                                                                               <  2:837251/150‑1 (MQ=255)

                                          aaaTCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGata                                                                                                            <  1:2298231/150‑1 (MQ=255)

                                            aTCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATc                                                                                                          <  1:2536613/150‑1 (MQ=255)

                                            aTCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATc                                                                                                          >  2:1010498/1‑150 (MQ=255)

                                              cACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATcgc                                                                                                        >  2:550215/1‑150 (MQ=255)

                                                cGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATcgccc                                                                                                      >  1:625941/1‑150 (MQ=255)

                                                cGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATcgccc                                                                                                      >  2:3705781/1‑150 (MQ=255)

                                                cGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATcgccc                                                                                                      <  2:2450175/150‑1 (MQ=255)

                                                        aTTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCccacca                                                                                              <  1:3116453/150‑1 (MQ=255)

                                                           tttAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGAt                                                                                           >  2:4347613/1‑150 (MQ=255)

                                                            ttAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATa                                                                                          <  1:481588/150‑1 (MQ=255)

                                                            ttAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATa                                                                                          >  1:4724918/1‑150 (MQ=255)

                                                            ttAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATa                                                                                          <  1:3910867/150‑1 (MQ=255)

                                                               aaTTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCg                                                                                       >  2:2213946/1‑150 (MQ=255)

                                                               aaTTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCg                                                                                       <  2:5057857/150‑1 (MQ=255)

                                                               aaTTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCg                                                                                       <  2:4074881/150‑1 (MQ=255)

                                                                aTTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGa                                                                                      <  2:417336/150‑1 (MQ=255)

                                                                 ttCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGAc                                                                                     <  1:2503385/150‑1 (MQ=255)

                                                                      aGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCa                                                                                <  2:3623934/150‑1 (MQ=255)

                                                                       ggCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAg                                                                               >  2:48644/1‑150 (MQ=255)

                                                                          gagaTGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACACTCAgcgc                                                                            >  2:1947941/1‑150 (MQ=255)

                                                                          gagaTGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAgcgc                                                                            >  1:3637464/1‑150 (MQ=255)

                                                                             gtGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGcgac                                                                         >  1:5072391/2‑150 (MQ=255)

                                                                             aTGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGcgac                                                                         >  1:2100801/1‑150 (MQ=255)

                                                                               gggCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGcgacga                                                                       <  2:2016657/150‑1 (MQ=255)

                                                                                ggCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGAt                                                                      <  1:4752925/150‑1 (MQ=255)

                                                                                ggCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGAt                                                                      <  1:665696/150‑1 (MQ=255)

                                                                                      tGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTcc                                                                <  2:1859044/150‑1 (MQ=255)

                                                                                      tGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTcc                                                                <  2:3381951/150‑1 (MQ=255)

                                                                                       gCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCg                                                               >  2:478942/1‑150 (MQ=255)

                                                                                        ccTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCgg                                                              <  2:2965848/150‑1 (MQ=255)

                                                                                         cTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGc                                                             >  2:2520490/1‑150 (MQ=255)

                                                                                          tCCAGACCGACAAAACACCAGAACATAACTGATAACTAAGAAAATAACCCGGTAAGTTAGATATTAACAGGTGCCGGAAATGGGATATTAGCAGGTTTGATATCGCCAGCACACCAGATATCGACAATCAGCGCGACGATAAGTCCGGCa                                                            >  1:4444599/1‑150 (MQ=255)

                                                                                              gACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATaa                                                        <  1:4316223/150‑1 (MQ=255)

                                                                                              gACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATaa                                                        >  1:357306/1‑150 (MQ=255)

                                                                                              gACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATaa                                                        >  2:2295480/1‑150 (MQ=255)

                                                                                              gACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATaa                                                        <  1:1725611/150‑1 (MQ=255)

                                                                                               aCCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAAc                                                       <  1:558471/150‑1 (MQ=255)

                                                                                                  gACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGt                                                    >  1:1122475/1‑150 (MQ=255)

                                                                                                  gACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGt                                                    >  1:4529992/1‑150 (MQ=255)

                                                                                                    cAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGttt                                                  <  1:236571/150‑1 (MQ=255)

                                                                                                    cAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGttt                                                  >  1:1912253/1‑150 (MQ=255)

                                                                                                     aaaaCACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTg                                                 <  2:1166610/150‑1 (MQ=255)

                                                                                                       aaCACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTa                                               <  1:2344337/150‑1 (MQ=255)

                                                                                                       aaCACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTa                                               >  2:2843115/1‑150 (MQ=255)

                                                                                                        acacCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAg                                              >  1:1733623/1‑150 (MQ=255)

                                                                                                         cacCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGa                                             >  1:756266/1‑150 (MQ=255)

                                                                                                         cacCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGa                                             >  1:835883/1‑150 (MQ=255)

                                                                                                          acCAGAACCTCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGAt                                            <  2:2120741/150‑1 (MQ=255)

                                                                                                             aGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAg                                         >  2:380199/1‑150 (MQ=255)

                                                                                                              gAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGc                                        <  1:1010498/150‑1 (MQ=255)

                                                                                                                aCATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCAc                                      <  1:268428/150‑1 (MQ=255)

                                                                                                                aCATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCAc                                      <  2:3499449/150‑1 (MQ=255)

                                                                                                                   tCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTgg                                   >  1:897309/1‑150 (MQ=255)

                                                                                                                    cACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGa                                  >  1:5016295/1‑150 (MQ=255)

                                                                                                                    cACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGa                                  >  2:1362813/1‑150 (MQ=255)

                                                                                                                      cTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAAc                                <  1:2988612/150‑1 (MQ=255)

                                                                                                                        gATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTg                              >  2:3217904/1‑150 (MQ=255)

                                                                                                                        gATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTg                              <  1:1410437/150‑1 (MQ=255)

                                                                                                                         aTAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTgg                             >  2:175356/1‑150 (MQ=255)

                                                                                                                         aTAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTgg                             >  2:715992/1‑150 (MQ=255)

                                                                                                                          tAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGc                            >  2:3914061/1‑150 (MQ=255)

                                                                                                                          tAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGc                            >  2:227320/1‑150 (MQ=255)

                                                                                                                            aCGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCAc                          >  1:3281351/1‑150 (MQ=255)

                                                                                                                             cGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCAcc                         <  1:4286217/150‑1 (MQ=255)

                                                                                                                              gcagcaAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACcg                        <  2:689927/150‑1 (MQ=255)

                                                                                                                                agcaAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACcgcg                      >  1:4552084/1‑150 (MQ=255)

                                                                                                                                     aTAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTAc                 <  1:1314951/150‑1 (MQ=255)

                                                                                                                                     aTAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTAc                 <  2:1329222/150‑1 (MQ=255)

                                                                                                                                       aaCCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCg               >  1:4158115/1‑150 (MQ=255)

                                                                                                                                       aaCCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCg               <  1:4084906/150‑1 (MQ=255)

                                                                                                                                        aCCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGa              <  2:5073824/150‑1 (MQ=255)

                                                                                                                                           cGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGatat           >  2:2913159/1‑150 (MQ=255)

                                                                                                                                           cGGTAAGTTAGATAATACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGAACCGatat           >  2:681885/1‑150 (MQ=255)

                                                                                                                                            ggTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGAtata          >  1:667077/1‑150 (MQ=255)

                                                                                                                                            ggTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACAAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGTAACTGGCACCGCGAGTACCGAtata          >  1:1593364/1‑150 (MQ=255)

                                                                                                                                              tAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGATATAcc        >  2:4124746/1‑150 (MQ=255)

                                                                                                                                              tAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGATATAcc        >  2:1567816/1‑150 (MQ=255)

                                                                                                                                                  ttCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGATATACCacac    >  1:2934075/1‑150 (MQ=255)

                                                                                                                                                    cGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGATATACCACAcca  >  2:3453169/1‑150 (MQ=255)

ACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGATATACCACACCA  >  CP009273/4359538‑4359835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`