| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,376,178 | C→A | 100% | E1004* (GAA→TAA) | mscM ← | mechanosensitive channel protein, miniconductance |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,376,178 | 0 | C | A | 99.0% | 1041.1 / ‑1.1 | 319 | E1004* (GAA→TAA) | mscM | mechanosensitive channel protein, miniconductance |
| Reads supporting (aligned to +/- strand): ref base C (3/1); new base A (181/134); total (184/135) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 323 total aligned reads displayed.
TACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGATGGTGGTGGCG > CP009273/4376031‑4376323 | tACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGACGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGc > 2:2377574/1‑150 (MQ=255)tACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGc < 1:2481798/150‑1 (MQ=255) aCGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCt < 1:6988/150‑1 (MQ=255) aCGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCt < 1:3506193/150‑1 (MQ=255) cGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTa < 1:308620/150‑1 (MQ=255) gACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTgg < 1:4109401/150‑1 (MQ=255) gACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTgg > 1:3271627/1‑150 (MQ=255) aCCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGc > 2:2684237/1‑150 (MQ=255) tctcAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGTAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCgg < 2:4288844/150‑1 (MQ=255) tctcAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCgg < 2:2531629/150‑1 (MQ=255) tctcAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCgg < 2:2961192/150‑1 (MQ=255) tctcAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCgg > 1:2844837/1‑150 (MQ=255) ctcAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGc > 1:1114499/1‑150 (MQ=255) ctcAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGc < 1:4233293/150‑1 (MQ=255) ctcAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGc < 2:2976843/150‑1 (MQ=255) cggcgTAAATACGCAGCTCGAAAATCTGGATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAgggg > 2:4177814/1‑150 (MQ=255) cggcgTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAgggg > 1:2159957/1‑150 (MQ=255) cgTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCg < 2:2199919/150‑1 (MQ=255) tAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGt > 1:1857779/1‑150 (MQ=255) aaTACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTAt < 2:4387184/150‑1 (MQ=255) aTACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATc > 1:2360397/1‑150 (MQ=255) aTACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATGTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATc > 2:308181/1‑150 (MQ=255) cGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTc < 1:906389/150‑1 (MQ=255) gCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCa < 1:2923071/150‑1 (MQ=255) aGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAac > 2:4772791/1‑150 (MQ=255) cTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAacac < 2:5128430/150‑1 (MQ=255) cTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAacac > 2:2803464/1‑150 (MQ=255) cTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAacac > 1:3210914/1‑150 (MQ=255) cTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAacac > 2:1802442/1‑150 (MQ=255) tCGAAAATCTGACTCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAAcacc < 1:4151552/150‑1 (MQ=255) cGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAAcacca < 2:4314470/150‑1 (MQ=255) cGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAAcacca > 2:2307260/1‑150 (MQ=255) aaaTCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCAcgc > 1:4797613/1‑150 (MQ=255) tGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTg < 1:2340210/150‑1 (MQ=255) gAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGa > 1:2733466/1‑150 (MQ=255) aTCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGAcc > 2:2471666/1‑150 (MQ=255) aTCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGAcc > 2:3645932/1‑150 (MQ=255) aTCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGGTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGAcc < 2:4664129/150‑1 (MQ=255) aTCCCCTGTGGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGAcc < 2:1943742/150‑1 (MQ=255) tCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCg < 2:1527566/150‑1 (MQ=255) ccccTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGa > 2:170810/1‑150 (MQ=255) cccTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAg > 2:3606753/1‑150 (MQ=255) tGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCa > 2:1386327/1‑150 (MQ=255) gTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCag < 2:2950066/150‑1 (MQ=255) gTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCag < 2:808749/150‑1 (MQ=255) ttGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCaga > 1:2706189/1‑150 (MQ=255) gCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGCGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCCCTTCTTAGCTATTGGCATCGGCAGGGGCCGGTCGCGGCACCACCCCGCGCGTGACCGAGTCagagc > 2:2277582/1‑149 (MQ=255) aGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGc > 2:3166587/1‑150 (MQ=255) gATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCg > 1:4527362/1‑150 (MQ=255) tCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCTGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGAc < 2:11767/150‑1 (MQ=255) ccaccaGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATATCCGTCACTTCTTAGCTATTCGCATCGGCAGGGGCCGGTCTCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGAcc > 1:5054235/1‑150 (MQ=255) caccaGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCa < 1:224687/150‑1 (MQ=255) caccaGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGGGACCGAGTCAGAGAGCGACCa < 2:1108929/150‑1 (MQ=255) accaGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAg > 1:1046655/1‑150 (MQ=255) accaGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAg > 1:3627178/1‑150 (MQ=255) caGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGtt > 2:3851093/1‑150 (MQ=255) caGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGtt > 2:1309989/1‑150 (MQ=255) caGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGtt < 1:3703771/150‑1 (MQ=255) gAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTggt < 1:3016113/150‑3 (MQ=255) gAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGAt < 2:4573303/150‑1 (MQ=255) aaGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATa > 2:3359288/1‑150 (MQ=255) aaGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATa > 1:3184379/1‑150 (MQ=255) aGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATaa < 1:2089307/150‑1 (MQ=255) tCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGc < 2:5060591/150‑1 (MQ=255) tCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGc > 1:2050985/1‑150 (MQ=255) ccGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCt < 1:3584570/150‑1 (MQ=255) ccGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCt < 1:4890318/150‑1 (MQ=255) ggTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCg > 1:718647/1‑150 (MQ=255) gTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCgg > 1:2436043/1‑150 (MQ=255) gTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTACCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCgg < 2:3010247/150‑1 (MQ=255) gggTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGtaata < 2:4356619/150‑1 (MQ=255) ttGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAAc < 2:2046013/150‑1 (MQ=255) cGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCt > 2:263914/1‑150 (MQ=255) cGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCt < 1:2564799/150‑1 (MQ=255) gATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCtt > 2:48140/1‑150 (MQ=255) aTCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTg < 1:2961220/150‑1 (MQ=255) aCCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTc < 1:1480138/150‑1 (MQ=255) aCCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTc < 1:3983863/150‑1 (MQ=255) ccAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCg < 1:263751/150‑1 (MQ=255) aGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGc > 1:3396054/1‑150 (MQ=255) aGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGc < 2:3906296/150‑1 (MQ=255) gAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACg > 2:4622207/1‑150 (MQ=255) gAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACg < 1:3767278/150‑1 (MQ=255) gcagcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGAt < 1:1685119/150‑1 (MQ=255) cGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTAtc < 2:1577070/150‑1 (MQ=255) cGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTAtc > 1:579426/1‑150 (MQ=255) cgcTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCttt < 2:3544626/150‑1 (MQ=255) cgcTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCttt > 2:4333035/1‑150 (MQ=255) gcTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTa < 1:3000826/150‑1 (MQ=255) cTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTAc < 1:1410295/150‑1 (MQ=255) tGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACg > 2:2607879/1‑150 (MQ=255) cGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGt > 1:3137980/1‑150 (MQ=255) tGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTccc > 1:4631345/1‑150 (MQ=255) aGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAg < 2:2165991/150‑1 (MQ=255) aTTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTg > 1:4127904/1‑150 (MQ=255) cGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtggt > 1:964597/1‑150 (MQ=255) cGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtggt > 2:1054349/1‑150 (MQ=255) aCTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtggtggtg < 2:604579/150‑1 (MQ=255) tcttaGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGATGGTGGTGgcg < 1:3166587/150‑1 (MQ=255) tcttaGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGATGGTGGTGgcg < 2:2570790/150‑1 (MQ=255) | TACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGATGGTGGTGGCG > CP009273/4376031‑4376323 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |