Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,381,504 T→A 100% I28I (ATT→ATA)  orn → oligoribonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,381,5040TA97.7% 986.4 / 7.9 301I28I (ATT→ATA) ornoligoribonuclease
Reads supporting (aligned to +/- strand):  ref base T (5/2);  new base A (168/126);  total (173/128)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 301 total aligned reads displayed.

CAAAACATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAG  >  CP009273/4381357‑4381649
                                                                                                                                                   |                                                                                                                                                 
cAAAACATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGc                                                                                                                                                 >  1:376645/1‑150 (MQ=255)
 aaaaCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGcc                                                                                                                                                >  1:3147412/1‑150 (MQ=255)
 aaaaCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGcc                                                                                                                                                <  2:577649/150‑1 (MQ=255)
        cTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTgg                                                                                                                                         >  1:3971219/1‑150 (MQ=255)
        cTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTgg                                                                                                                                         >  1:2422091/1‑150 (MQ=255)
         tATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGt                                                                                                                                        <  2:633768/150‑1 (MQ=255)
         tATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGt                                                                                                                                        <  2:1546428/150‑1 (MQ=255)
          aTGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTg                                                                                                                                       >  2:5105888/1‑150 (MQ=255)
             aTACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGAcc                                                                                                                                    >  1:4593206/1‑150 (MQ=255)
              tACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCg                                                                                                                                   <  2:26510/150‑1 (MQ=255)
                cacGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGAt                                                                                                                                 <  1:632211/150‑1 (MQ=255)
                cacGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGAt                                                                                                                                 <  1:2772062/150‑1 (MQ=255)
                cacGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGAt                                                                                                                                 <  2:2118908/150‑1 (MQ=255)
                    cAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCa                                                                                                                             <  2:4743594/150‑1 (MQ=255)
                     aaTTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCACTGAAAACAAGCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCAGTATTGAGATAGCCACGCGGGTGACCGATGccca                                                                                                                            >  2:4414199/1‑148 (MQ=255)
                        tgtgGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCt                                                                                                                         <  1:3909041/150‑1 (MQ=255)
                        tgtgGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCt                                                                                                                         <  2:365681/150‑1 (MQ=255)
                           ggATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGaa                                                                                                                      >  2:1908173/1‑150 (MQ=255)
                               cAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATAtt                                                                                                                  <  2:2727247/150‑1 (MQ=255)
                                aaTATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTc                                                                                                                 >  1:1292466/1‑150 (MQ=255)
                                 atatAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCt                                                                                                                <  2:1014822/150‑1 (MQ=255)
                                 atatAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCt                                                                                                                <  1:3680755/150‑1 (MQ=255)
                                  tataGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTg                                                                                                               <  2:3582206/150‑1 (MQ=255)
                                    taGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCGGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGc                                                                                                             <  2:5072218/150‑1 (MQ=255)
                                      gTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCCACCTGAATATTCTGGCAg                                                                                                           >  2:4654089/1‑150 (MQ=255)
                                      gTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAg                                                                                                           <  2:3320287/150‑1 (MQ=255)
                                      gTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAg                                                                                                           >  2:3501571/1‑150 (MQ=255)
                                       tCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGa                                                                                                          <  2:4901962/150‑1 (MQ=255)
                                         aCTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAg                                                                                                        >  2:1677098/1‑150 (MQ=255)
                                         aCTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAg                                                                                                        >  2:4254046/1‑150 (MQ=255)
                                             tgAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGcc                                                                                                    >  1:1595779/1‑150 (MQ=255)
                                             tgAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGcc                                                                                                    >  2:2850401/1‑150 (MQ=255)
                                              gAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCg                                                                                                   >  1:24349/1‑150 (MQ=255)
                                                 tGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGAcc                                                                                                >  1:378830/1‑150 (MQ=255)
                                                   ggTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCAt                                                                                              >  1:398416/1‑150 (MQ=255)
                                                    gTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCAtt                                                                                             <  1:3163940/150‑1 (MQ=255)
                                                     tGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTg                                                                                            >  1:4771783/1‑150 (MQ=255)
                                                       gAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCa                                                                                          >  1:4019795/1‑150 (MQ=255)
                                                        aaaaTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTGGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAg                                                                                         <  2:3174485/150‑1 (MQ=255)
                                                          aaTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTa                                                                                       >  1:3505886/1‑150 (MQ=255)
                                                           aTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTac                                                                                      <  1:3265775/150‑1 (MQ=255)
                                                             aGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCGGGCAGAAGGGCCGACCATTGCAGTacac                                                                                    <  1:2981797/150‑1 (MQ=255)
                                                              gctttaGTTCCAATGAAAAAAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCTATCGCATTATTGAGATAGCCAAGCTGGTGACCGATTCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACAcc                                                                                   <  2:2484106/145‑1 (MQ=255)
                                                              gCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACAcc                                                                                   >  1:80220/1‑150 (MQ=255)
                                                               cATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCa                                                                                  >  1:3361887/1‑150 (MQ=255)
                                                                aTGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAg                                                                                 >  1:572869/1‑150 (MQ=255)
                                                                   aGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCt                                                                              <  2:3975371/150‑1 (MQ=255)
                                                                   aGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCt                                                                              >  1:5040810/1‑150 (MQ=255)
                                                                      gCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCtgat                                                                           <  1:1704181/150‑1 (MQ=255)
                                                                      gCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCGGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCtgat                                                                           <  2:1054713/150‑1 (MQ=255)
                                                                       ccAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCtgatg                                                                          >  2:2244570/1‑150 (MQ=255)
                                                                        cAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCtgatga                                                                         >  2:2917838/1‑150 (MQ=255)
                                                                        cAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCtgatga                                                                         >  1:3647907/1‑150 (MQ=255)
                                                                        cAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCtgatga                                                                         >  1:4362570/1‑150 (MQ=255)
                                                                           tGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACa                                                                      <  2:1400032/150‑1 (MQ=255)
                                                                            gAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAg                                                                     >  1:3203303/1‑150 (MQ=255)
                                                                            gAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAg                                                                     >  2:4562959/1‑150 (MQ=255)
                                                                              aaaCAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCt                                                                   >  2:3136854/1‑150 (MQ=255)
                                                                              aaaCAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCt                                                                   >  2:2940148/1‑150 (MQ=255)
                                                                               aacaacCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTg                                                                  >  2:3803507/1‑150 (MQ=255)
                                                                                  aacCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGgcg                                                               >  1:2058810/1‑150 (MQ=255)
                                                                                    ccTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCt                                                             >  1:3222034/1‑150 (MQ=255)
                                                                                    ccTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCt                                                             >  1:4565048/1‑150 (MQ=255)
                                                                                                 gatcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCTGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTgg                                                >  1:2270715/1‑150 (MQ=255)
                                                                                                 gatcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTgg                                                >  2:1461340/1‑150 (MQ=255)
                                                                                                 gatcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTgg                                                >  1:1025021/1‑150 (MQ=255)
                                                                                                 gatcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACAATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTgg                                                >  2:4582057/1‑150 (MQ=255)
                                                                                                   tcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGaa                                              <  2:3397611/150‑1 (MQ=255)
                                                                                                    cTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAAc                                             >  1:221130/1‑150 (MQ=255)
                                                                                                       gagaTGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTg                                          >  1:3348692/1‑150 (MQ=255)
                                                                                                         gaTGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTgcg                                        <  1:2641667/150‑1 (MQ=255)
                                                                                                         gaTGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTgcg                                        >  2:2790690/1‑150 (MQ=255)
                                                                                                           tGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCa                                      >  2:2580395/1‑150 (MQ=255)
                                                                                                           tGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCa                                      >  2:1186854/1‑150 (MQ=255)
                                                                                                            gACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCAc                                     >  1:987599/1‑150 (MQ=255)
                                                                                                             aCCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCAcc                                    >  1:4699347/1‑150 (MQ=255)
                                                                                                              ccGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCAccc                                   <  2:4782212/150‑1 (MQ=255)
                                                                                                              ccGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCAccc                                   >  2:3354565/1‑150 (MQ=255)
                                                                                                               cGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCa                                  >  2:4292431/1‑150 (MQ=255)
                                                                                                                  tCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATAc                               <  2:4075316/150‑1 (MQ=255)
                                                                                                                   cTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATAcc                              >  2:2279440/1‑150 (MQ=255)
                                                                                                                   cTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATAcc                              <  1:21841/150‑1 (MQ=255)
                                                                                                                    tGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCg                             >  1:1488474/1‑150 (MQ=255)
                                                                                                                      gATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGcc                           >  2:1082547/1‑150 (MQ=255)
                                                                                                                       aTCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCa                          <  2:1509699/150‑1 (MQ=255)
                                                                                                                        tCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAg                         >  1:3825043/1‑150 (MQ=255)
                                                                                                                          ccGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCg                       <  1:1987460/150‑1 (MQ=255)
                                                                                                                           cGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCgg                      <  2:4371570/150‑1 (MQ=255)
                                                                                                                            gAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCggg                     >  2:2555252/1‑150 (MQ=255)
                                                                                                                               cgcgATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTg                  <  1:1877141/150‑1 (MQ=255)
                                                                                                                               cgcgATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTg                  >  1:3290933/1‑150 (MQ=255)
                                                                                                                                 cgATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGt                <  1:4156902/150‑1 (MQ=255)
                                                                                                                                  gATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTa               <  1:1504944/150‑1 (MQ=255)
                                                                                                                                  gATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTa               <  2:4808568/150‑1 (MQ=255)
                                                                                                                                   aTCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTag              >  1:1139783/1‑150 (MQ=255)
                                                                                                                                     cGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTagag            <  2:1338749/150‑1 (MQ=255)
                                                                                                                                       cATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAgcg          >  1:2561335/1‑150 (MQ=255)
                                                                                                                                          tattGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGt       >  2:2155075/1‑150 (MQ=255)
                                                                                                                                           attGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTg      <  2:3222034/150‑1 (MQ=255)
                                                                                                                                               agaTAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAg  >  1:2099607/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CAAAACATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAG  >  CP009273/4381357‑4381649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑