Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,388,902 G→T 100% A558A (GCG→GCT)  mutL → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,388,9020GT100.0% 945.3 / ‑6.7 282A558A (GCG→GCT) mutLmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (158/123);  minor base A (1/0);  total (159/123)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 282 total aligned reads displayed.

ATTGGGTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATC  >  CP009273/4388755‑4389047
                                                                                                                                                   |                                                                                                                                                 
aTTGGGTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCa                                                                                                                                                 <  2:752948/150‑1 (MQ=255)
   gggTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCATTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTg                                                                                                                                              <  2:3202844/150‑1 (MQ=255)
     gTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGTCAGTGACTTTAACCTTACGACAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGa                                                                                                                                            <  2:1819295/150‑1 (MQ=255)
     gTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGa                                                                                                                                            <  2:1381428/150‑1 (MQ=255)
      tATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGAt                                                                                                                                           <  1:2036613/150‑1 (MQ=255)
       aTTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGAtt                                                                                                                                          >  1:1664426/1‑150 (MQ=255)
        ttGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTg                                                                                                                                         <  2:1498308/150‑1 (MQ=255)
        ttGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTg                                                                                                                                         >  1:324165/1‑150 (MQ=255)
         tGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGc                                                                                                                                        >  1:728266/1‑150 (MQ=255)
         tGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGc                                                                                                                                        >  2:2811069/1‑150 (MQ=255)
          gATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCa                                                                                                                                       >  1:5138437/1‑150 (MQ=255)
             ttCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGa                                                                                                                                    >  1:4383762/1‑150 (MQ=255)
                 aGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATc                                                                                                                                <  2:4298875/150‑1 (MQ=255)
                 aGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATc                                                                                                                                >  2:3928978/1‑150 (MQ=255)
                 aGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATc                                                                                                                                <  2:4687748/150‑1 (MQ=255)
                  gTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCt                                                                                                                               >  1:2413664/1‑150 (MQ=255)
                  gTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCt                                                                                                                               <  1:3088878/150‑1 (MQ=255)
                  gTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCt                                                                                                                               >  1:1422945/1‑150 (MQ=255)
                   tCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCtg                                                                                                                              <  2:3006737/150‑1 (MQ=255)
                    cAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCtga                                                                                                                             <  1:3614924/150‑1 (MQ=255)
                    cAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCtga                                                                                                                             <  1:1757875/150‑1 (MQ=255)
                     aGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCtgat                                                                                                                            >  1:1285217/1‑150 (MQ=255)
                      gATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCtgatg                                                                                                                           <  2:4015238/150‑1 (MQ=255)
                          cacaGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCg                                                                                                                       <  1:4000061/150‑1 (MQ=255)
                          cacaGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCg                                                                                                                       <  2:1316059/150‑1 (MQ=255)
                           acaGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGa                                                                                                                      <  1:5000944/150‑1 (MQ=255)
                            caGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGaa                                                                                                                     <  1:4594992/150‑1 (MQ=255)
                              gCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACa                                                                                                                   <  1:2343844/150‑1 (MQ=255)
                              gCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACa                                                                                                                   <  1:2399148/150‑1 (MQ=255)
                                aTGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATg                                                                                                                 >  1:98980/1‑150 (MQ=255)
                                aTGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATg                                                                                                                 >  1:912049/1‑150 (MQ=255)
                                     aCCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAg                                                                                                            <  1:956083/150‑1 (MQ=255)
                                      ccATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGt                                                                                                           >  2:3103155/1‑150 (MQ=255)
                                       cATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTg                                                                                                          <  1:3330501/150‑1 (MQ=255)
                                       cATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTg                                                                                                          <  2:1475974/150‑1 (MQ=255)
                                             ggCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAAt                                                                                                    >  2:3622204/1‑150 (MQ=255)
                                             ggCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAAt                                                                                                    >  2:1180314/1‑150 (MQ=255)
                                             ggCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCAGGCGAAGCAGTCCGGATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGACCATGCGCAGTGGTCAAt                                                                                                    >  2:1908415/1‑150 (MQ=255)
                                              gCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATg                                                                                                   <  1:4430150/150‑1 (MQ=255)
                                                aGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGc                                                                                                 >  2:4555451/1‑150 (MQ=255)
                                                aGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGc                                                                                                 <  2:3282716/150‑1 (MQ=255)
                                                   gCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGcaca                                                                                              >  2:4630393/1‑150 (MQ=255)
                                                   gCCTTTACCCGTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGcaca                                                                                              <  2:2936362/150‑1 (MQ=255)
                                                        tACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCa                                                                                         <  1:2170920/150‑1 (MQ=255)
                                                          cccTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATa                                                                                       >  2:2663/1‑150 (MQ=255)
                                                              tACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAAGATTGCTCAGTGGATTGCACGAAATCTGATGAGCGACCATGCGCAGTGGTCACTGGCACAGGCCATAAccc                                                                                   >  2:1698483/1‑150 (MQ=255)
                                                               aCGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCct                                                                                  <  1:1069340/150‑1 (MQ=255)
                                                               aCGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCct                                                                                  >  1:2620016/1‑150 (MQ=255)
                                                               aCGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCct                                                                                  <  1:1485839/150‑1 (MQ=255)
                                                                 gCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgc                                                                                >  1:365162/1‑150 (MQ=255)
                                                                 gCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgc                                                                                >  1:2013326/1‑150 (MQ=255)
                                                                  ccAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgct                                                                               <  2:2308506/150‑1 (MQ=255)
                                                                   caacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgctg                                                                              >  2:2136703/1‑150 (MQ=255)
                                                                    aacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTgg                                                                             >  1:4907123/1‑150 (MQ=255)
                                                                     acaaAATTTACAAATCTTGATTCCTGAACTGATAGGCGACCTGGCGTAGCAGTCCGTATTCGAACCTGGCAATATTGCTCCGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGc                                                                            <  2:1657954/150‑1 (MQ=255)
                                                                        aaaTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGa                                                                         <  1:4630393/150‑1 (MQ=255)
                                                                         aaTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGAc                                                                        >  1:3398494/1‑150 (MQ=255)
                                                                           tttACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGt                                                                      <  1:4184704/150‑1 (MQ=255)
                                                                           tttACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGt                                                                      >  1:3723978/1‑150 (MQ=255)
                                                                            ttACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTg                                                                     >  1:1182735/1‑150 (MQ=255)
                                                                             tACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTgg                                                                    >  2:1001599/1‑150 (MQ=255)
                                                                                 aaTCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACg                                                                >  1:2664163/1‑150 (MQ=255)
                                                                                    cTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGtt                                                             >  2:1590519/1‑150 (MQ=255)
                                                                                    cTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGtt                                                             >  2:1334947/1‑150 (MQ=255)
                                                                                     ttGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTa                                                            <  2:3215909/150‑1 (MQ=255)
                                                                                     ttGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTa                                                            >  1:2724299/1‑150 (MQ=255)
                                                                                      tGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTAt                                                           <  1:3622204/150‑1 (MQ=255)
                                                                                          tCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTCTGTcc                                                       >  2:903918/1‑150 (MQ=255)
                                                                                                   gATAGGCTACCTTGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTtgt                                              >  2:4735804/1‑150 (MQ=255)
                                                                                                    aTAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTtgtg                                             >  2:2463888/1‑150 (MQ=255)
                                                                                                    aTAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTtgtg                                             >  1:3943217/1‑150 (MQ=255)
                                                                                                     tAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGa                                            >  2:179636/1‑150 (MQ=255)
                                                                                                     tAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGa                                            >  2:3184770/1‑150 (MQ=255)
                                                                                                       ggCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGaaa                                          <  2:4203216/150‑1 (MQ=255)
                                                                                                             cTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgccg                                    >  2:286586/1‑150 (MQ=255)
                                                                                                             cTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgccg                                    >  1:4214239/1‑150 (MQ=255)
                                                                                                              tGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAagccgc                                   >  2:713839/1‑150 (MQ=255)
                                                                                                               ggCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTCTGTCCGCAACTTGTGAAAACgccgcc                                  >  2:554382/1‑150 (MQ=255)
                                                                                                               ggCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACgccgcc                                  >  1:4109044/1‑150 (MQ=255)
                                                                                                                 cGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCgg                                >  1:1047357/1‑150 (MQ=255)
                                                                                                                 cGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCgg                                >  1:669963/1‑150 (MQ=255)
                                                                                                                 cGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCgg                                >  1:3643515/1‑150 (MQ=255)
                                                                                                                   aaGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggt                              <  1:4532990/150‑1 (MQ=255)
                                                                                                                    aGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGTTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtg                             <  1:3010290/150‑1 (MQ=255)
                                                                                                                      cAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtggt                           >  2:84607/1‑150 (MQ=255)
                                                                                                                      cAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtggt                           >  1:3516854/1‑150 (MQ=255)
                                                                                                                      cAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtggt                           >  1:3547449/1‑150 (MQ=255)
                                                                                                                        gTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCt                         <  2:3472747/150‑1 (MQ=255)
                                                                                                                         tCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTg                        >  1:2959410/1‑150 (MQ=255)
                                                                                                                          ccGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGt                       >  1:2843744/1‑150 (MQ=255)
                                                                                                                            gTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGACCGGTGCTGTCCGCAACGTGTGAAAACGCCGCCGGGTGGTCTGTTa                     >  2:4325828/1‑150 (MQ=255)
                                                                                                                             tATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTAc                    >  1:2341441/1‑150 (MQ=255)
                                                                                                                                tCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAAt                 >  2:3802163/1‑150 (MQ=255)
                                                                                                                                  gAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCt               <  2:3416484/150‑1 (MQ=255)
                                                                                                                                  gAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCt               <  2:4989386/150‑1 (MQ=255)
                                                                                                                                        gtcAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTTCAATCTGTTGAt         <  2:2440701/148‑1 (MQ=255)
                                                                                                                                        ggCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGAt         >  1:4422811/1‑150 (MQ=255)
                                                                                                                                          cAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGAttt       <  1:3846493/150‑1 (MQ=255)
                                                                                                                                          cAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGAttt       >  2:4102098/1‑150 (MQ=255)
                                                                                                                                          cAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGAttt       <  1:5029062/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATTGGGTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATC  >  CP009273/4388755‑4389047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑