BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,402,665	T→G	6.5%	G154G (GGT→GGG)	yjfM →	DUF1190 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,402,665	0	T	G	6.5%	659.7 / 13.1	256	G154G (GGT→GGG)	yjfM	DUF1190 family protein
Reads supporting (aligned to +/- strand): ref base T (89/145); new base G (18/3); total (108/148)
Fisher's exact test for biased strand distribution p-value = 2.91e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

AATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTT  >  CP009273/4402516‑4402813

   atGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGATTTTTTTTAA‑GCCGGGGTATTAGTAAAGATAGCGATGAGCCGTTTTTTTATAACAGCGGCGGTTcc                                                                                                                                                   >  2:4148880/1‑150 (MQ=255)

   atGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATAGCGATGAGCCGTTTGTTTATAAAATCGGCGGGTcc                                                                                                                                                   >  2:2842950/1‑150 (MQ=255)

   atGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGATTTTTGTTAA‑GCCGGGGTATTCGTTAAGATCGCGATGAGCCGTTTGTTTTTAAAAGCGGCGGGTcc                                                                                                                                                   >  2:2908766/1‑150 (MQ=255)

      aCTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCctct                                                                                                                                                <  1:380875/150‑1 (MQ=255)

        tCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGGGTGGGTTTTTGTTAA‑TCCGGGTTATTGGTAAATATCGCGCTGTTCCGGTTTTTTATAAAAGCGGCGGGTCCTCttt                                                                                                                                              >  2:2248601/1‑150 (MQ=255)

         cagcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGCTTTTTTTTAA‑TCCGGGGTATTCGTAAATATAGCGATGAGCCGTTTGTTTATAAAAGCCGCCGGTACTCattt                                                                                                                                             >  2:3165067/1‑150 (MQ=255)

          agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTg                                                                                                                                            <  2:1586258/150‑1 (MQ=255)

             agAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCtt                                                                                                                                         <  1:2913550/150‑1 (MQ=255)

             agAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTATGGATTTTTGTTTA‑TCCGGGTTATTAGTAAATATAGCGCTGAGCCGTTTGTTTTTAAAAGCGGCGGGTCCACtttttgtt                                                                                                                                         >  2:1953011/1‑146 (MQ=255)

                 cTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTcgcg                                                                                                                                     <  1:1255133/150‑1 (MQ=255)

                 cTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTcgcg                                                                                                                                     <  2:2409548/150‑1 (MQ=255)

                 cTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGATTTTTTTTTT‑TACGGGTTTTTAGTTAAGATAGCGCTGTGCCGGTTGTTTTTTAAATCGGGGGGTACGCTTTTTTTTcgcg                                                                                                                                     >  1:648236/1‑150 (MQ=255)

                     cAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTTGTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGGTCCTCTTTTGCTTCGCGCCCa                                                                                                                                 >  2:311237/1‑150 (MQ=255)

                     cAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCa                                                                                                                                 <  2:344509/150‑1 (MQ=255)

                     cAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCa                                                                                                                                 <  2:4893048/150‑1 (MQ=255)

                     cAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGATTTTTTGTTA‑GCCGGGGTATTCGTAAAGATCGCGATGAGCCGGTTGTTTATAACAGCGGCGGTTCCTCATTTTCTTCGCGCCCa                                                                                                                                 >  2:1025735/1‑150 (MQ=255)

                      aGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAg                                                                                                                                <  2:3722005/150‑1 (MQ=255)

                        tCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTc                                                                                                                              <  2:2136827/150‑1 (MQ=255)

                        tCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTc                                                                                                                              <  2:4705153/150‑1 (MQ=255)

                         cTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCt                                                                                                                             <  2:4913538/150‑1 (MQ=255)

                         cTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGGGGGGTTTTTTTTTTT‑GGCGGGGTATTGGTAAATATCGCGATTTGCCGGTTTTTTTTAAAAGCGGCGGGTGCGCGTTTGGTTGGCGCCCActct                                                                                                                             >  1:3366899/1‑150 (MQ=255)

                             gTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGgcg                                                                                                                         <  2:1252523/150‑1 (MQ=255)

                                cGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGATTTTTGTTAA‑TCCGGGTTATTCGTAAAGATCGCGCTGATCCGTTTGTTTATAACAGCGGCGGTTAAGCTTTTTCTTCGCGCCCAGTCTGGCgcag                                                                                                                      >  2:4412378/1‑150 (MQ=255)

                                  aaaaTTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgcagca                                                                                                                    <  2:1878668/150‑1 (MQ=255)

                                  aaaaGTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTGTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgcagca                                                                                                                    <  2:4384946/150‑1 (MQ=255)

                                    taTTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGCTTTTTTTTAATGCCGGG‑TATTAGTAAATATAGCGATTATGCGGTTGTTTTTAAAACCCGCGGGTTCAAGTTTTAGTAGCCCCCACTCTGGCGCCGCAca                                                                                                                  >  2:3525312/2‑149 (MQ=255)

                                     aTTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACtt                                                                                                                 <  2:68515/150‑1 (MQ=255)

                                        gCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTg                                                                                                              <  2:3936259/150‑1 (MQ=255)

                                           actacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTg                                                                                                           <  2:541309/150‑1 (MQ=255)

                                             tacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAt                                                                                                         <  2:4125542/150‑1 (MQ=255)

                                              acGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGGTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAtt                                                                                                        <  1:3992846/150‑1 (MQ=255)

                                                 aCAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTTTTAA‑GCCGGGTTATTCGTAAAGATAGCGATTAGCCCGTTGTTTATAAAAGCGGCGGTTCCTCTTTTTCTTCGCGCCCACTCTGGCGCAGCACATCAGGTGATTaat                                                                                                     >  2:3380717/1‑148 (MQ=255)

                                                     tGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTg                                                                                                 <  1:2699602/150‑1 (MQ=255)

                                                     tGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTg                                                                                                 <  1:1537461/150‑1 (MQ=255)

                                                     tGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTg                                                                                                 <  1:4227752/150‑1 (MQ=255)

                                                       ttGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCTATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGgc                                                                                               <  1:1851488/150‑1 (MQ=255)

                                                          aaCAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCt                                                                                            <  1:4960148/150‑1 (MQ=255)

                                                            cAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTcc                                                                                          <  1:4472620/150‑1 (MQ=255)

                                                            cAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCACAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTcc                                                                                          <  2:2416332/150‑1 (MQ=255)

                                                            cAGAGTTGGATCCCGGTCGTTTCTGGATTATTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTcc                                                                                          <  1:2620798/150‑1 (MQ=255)

                                                             agagTTGGATCCCGGTCGTGTCTGGATTTTTGTTAA‑TCCGGGGTATTCGTAAACATCGCGATGAGCCGGGTGTTTATAAAACCGGCGGTTACTCTTTTGCTTCGCGCCCACACTGGCGCAGCCCGTCTGGTGATTACTCCTGGCGCTccc                                                                                         >  2:4799086/1‑149 (MQ=255)

                                                                  tGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTGTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGGTCt                                                                                    <  1:1620649/150‑1 (MQ=255)

                                                                   ggATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTg                                                                                   <  2:2360190/150‑1 (MQ=255)

                                                                   ggATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTg                                                                                   <  2:3938897/150‑1 (MQ=255)

                                                                    gATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTgg                                                                                  <  1:4129139/150‑1 (MQ=255)

                                                                    gATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTgg                                                                                  <  1:3652699/150‑1 (MQ=255)

                                                                        ccGGTCGTTTCTGGATTTTTTTTAA‑GCCGGGTTATTAGTAAATATAGCGATGAGCCGTTTTTTTATAAAACCGGCGGTTACTCTTTTTCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTAATACAGGCGCTCCGGTTCTGGCaaa                                                                              >  2:2698510/1‑150 (MQ=255)

                                                                         cGGTCGTTTCAGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTCTAACAGCGGCGGTTCCTCTTTTGCGTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCGCCGGTTCTGGCcaaa                                                                             >  2:2636509/1‑150 (MQ=255)

                                                                             cGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAaga                                                                         >  2:3825587/1‑150 (MQ=255)

                                                                              gTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAagag                                                                        >  2:1809952/1‑150 (MQ=255)

                                                                                ttCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTc                                                                      <  2:3366705/150‑1 (MQ=255)

                                                                                  cTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCtt                                                                    <  2:3251347/150‑1 (MQ=255)

                                                                                  cTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCtt                                                                    >  1:1715519/1‑150 (MQ=255)

                                                                                  cTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCtt                                                                    <  2:5154462/150‑1 (MQ=255)

                                                                                   tGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTa                                                                   <  1:2798379/150‑1 (MQ=255)

                                                                                     gATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTAct                                                                 >  2:280861/1‑150 (MQ=255)

                                                                                     gATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTAct                                                                 <  2:1861880/150‑1 (MQ=255)

                                                                                      aTTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGGGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTActc                                                                >  2:2068971/1‑150 (MQ=255)

                                                                                        ttttGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCtt                                                              <  1:3168649/150‑1 (MQ=255)

                                                                                         tttGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTc                                                             <  1:1500826/150‑1 (MQ=255)

                                                                                           tGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCgg                                                           >  2:2828529/1‑150 (MQ=255)

                                                                                           tGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCgg                                                           >  1:2200953/1‑150 (MQ=255)

                                                                                            gTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCggg                                                          >  1:1858261/1‑150 (MQ=255)

                                                                                            gTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCggg                                                          >  2:4221371/1‑150 (MQ=255)

                                                                                            gTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCggg                                                          <  1:1101242/150‑1 (MQ=255)

                                                                                              tAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGggcg                                                        <  2:4051102/150‑1 (MQ=255)

                                                                                                   ccGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCtt                                                    >  2:88216/1‑150 (MQ=255)

                                                                                                    cGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCttt                                                   <  2:2043020/150‑1 (MQ=255)

                                                                                                    cGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCttt                                                   <  1:4563207/150‑1 (MQ=255)

                                                                                                      ggTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTac                                                 <  1:3830640/150‑1 (MQ=255)

                                                                                                        ttATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTacca                                               >  2:1107470/1‑150 (MQ=255)

                                                                                                        ttATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTacca                                               <  2:1321198/150‑1 (MQ=255)

                                                                                                                aaaGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCaaaaaa                                       <  2:840838/150‑1 (MQ=255)

                                                                                                                aaaGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCaaaaaa                                       >  1:5082044/1‑150 (MQ=255)

                                                                                                                 aaGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAg                                      >  1:3218021/1‑150 (MQ=255)

                                                                                                                 aaGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAg                                      >  1:1882980/1‑150 (MQ=255)

                                                                                                                  aGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGc                                     <  1:4910560/150‑1 (MQ=255)

                                                                                                                      cgcgATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCt                                 >  1:4097963/1‑150 (MQ=255)

                                                                                                                        cgATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTAc                               >  2:74972/1‑150 (MQ=255)

                                                                                                                        cgATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTAc                               >  1:3727121/1‑150 (MQ=255)

                                                                                                                          aTGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCg                             >  2:253898/1‑150 (MQ=255)

                                                                                                                           tGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGt                            >  1:838384/1‑150 (MQ=255)

                                                                                                                               ccGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTtct                        <  1:2064617/150‑1 (MQ=255)

                                                                                                                               ccGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTtct                        <  1:4646298/150‑1 (MQ=255)

                                                                                                                                cGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTtctc                       <  2:3627454/150‑1 (MQ=255)

                                                                                                                                      tttATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCgcggcg                 <  1:3892193/150‑1 (MQ=255)

                                                                                                                                      tttATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCgcggcg                 <  1:3380717/150‑1 (MQ=255)

                                                                                                                                       ttATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGcggcgg                >  1:351956/1‑150 (MQ=255)

                                                                                                                                         ataACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCt              >  1:2422084/1‑150 (MQ=255)

                                                                                                                                          taACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAACAGCGTCTACCGTTTCTCGCGGCGGCTa             <  1:4754159/150‑1 (MQ=255)

                                                                                                                                          taACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTa             >  1:187573/1‑150 (MQ=255)

                                                                                                                                          taACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTa             >  1:332121/1‑150 (MQ=255)

                                                                                                                                          taACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTa             >  2:1673615/1‑150 (MQ=255)

                                                                                                                                               gcggcgGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTc        <  2:1427236/150‑1 (MQ=255)

                                                                                                                                                cggcggTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCg       >  1:5034135/1‑150 (MQ=255)

                                                                                                                                                  gcggTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCGGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGtt     <  1:523219/150‑1 (MQ=255)

                                                                                                                                                    ggTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGttct   <  1:203676/150‑1 (MQ=255)

                                                                                                                                                    ggTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGttct   >  2:3926982/1‑150 (MQ=255)

                                                                                                                                                     gTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGttctt  >  2:1643296/1‑150 (MQ=255)

                                                                                                                                                     gTTCCTCTTTTGCGTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGttctt  <  1:2898490/150‑1 (MQ=255)

AATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAA‑GCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTT  >  CP009273/4402516‑4402813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`