Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,406,553 C→A 100% R12L (CGG→CTG)  bsmA ← bioflm peroxide resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,406,5530CA98.0% 799.4 / 3.7 249R12L (CGG→CTG) bsmAbioflm peroxide resistance protein
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base A (110/134);  total (113/136)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 249 total aligned reads displayed.

GCTTACGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGA  >  CP009273/4406408‑4406699
                                                                                                                                                 |                                                                                                                                                  
 cTTACGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTaa                                                                                                                                               <  2:1548472/150‑1 (MQ=255)
 cTTACGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTaa                                                                                                                                               >  2:628597/1‑150 (MQ=255)
 cTTACGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTaa                                                                                                                                               <  2:3222440/150‑1 (MQ=255)
   tACGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAAt                                                                                                                                             <  2:4615568/150‑1 (MQ=255)
    aCGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATa                                                                                                                                            <  2:4401955/150‑1 (MQ=255)
      gCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAAc                                                                                                                                          <  2:2654313/150‑1 (MQ=255)
       cTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACg                                                                                                                                         >  1:2672107/1‑150 (MQ=255)
        tACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGc                                                                                                                                        <  1:576845/150‑1 (MQ=255)
         aCCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCt                                                                                                                                       <  1:3203143/150‑1 (MQ=255)
          ccTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTa                                                                                                                                      <  1:3502429/150‑1 (MQ=255)
          ccTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTa                                                                                                                                      <  1:1464087/150‑1 (MQ=255)
              ttCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTAc                                                                                                                                  >  1:2301034/1‑150 (MQ=255)
                  cTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGttt                                                                                                                              <  2:1118591/150‑1 (MQ=255)
                   tGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTc                                                                                                                             <  2:1819545/150‑1 (MQ=255)
                     tAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCt                                                                                                                           <  1:4619933/150‑1 (MQ=255)
                      aaCCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTg                                                                                                                          >  2:2353739/1‑150 (MQ=255)
                      aaCCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTg                                                                                                                          >  2:2247158/1‑150 (MQ=255)
                          cTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTaa                                                                                                                      <  1:5005535/150‑1 (MQ=255)
                          cTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTaa                                                                                                                      >  2:3673/1‑150 (MQ=255)
                          cTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTaa                                                                                                                      >  1:357865/1‑150 (MQ=255)
                           ttGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAAc                                                                                                                     <  2:4020688/150‑1 (MQ=255)
                             gCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCGTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCa                                                                                                                   <  1:4896220/150‑1 (MQ=255)
                               gTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAta                                                                                                                 <  2:47655/150‑1 (MQ=255)
                                tCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtag                                                                                                                >  1:1566977/1‑150 (MQ=255)
                                tCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtag                                                                                                                >  2:4182975/1‑150 (MQ=255)
                                 cTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagt                                                                                                               >  2:867506/1‑150 (MQ=255)
                                  tGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtg                                                                                                              >  1:3729348/1‑150 (MQ=255)
                                  tGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtg                                                                                                              <  1:1921756/150‑1 (MQ=255)
                                   ggTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgt                                                                                                             <  2:3363609/150‑1 (MQ=255)
                                       gcggcgAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgtagtg                                                                                                         <  1:813601/150‑1 (MQ=255)
                                         ggcgAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGcc                                                                                                       <  2:4741541/150‑1 (MQ=255)
                                          gcgAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCt                                                                                                      <  2:5161192/150‑1 (MQ=255)
                                           cgAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACTCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCtt                                                                                                     <  1:2036257/150‑1 (MQ=255)
                                           cgAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCtt                                                                                                     <  1:2440936/150‑1 (MQ=255)
                                            gAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCtta                                                                                                    >  2:2263550/1‑150 (MQ=255)
                                            gAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCtta                                                                                                    >  1:910309/1‑150 (MQ=255)
                                             aaTCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCttat                                                                                                   >  1:332050/1‑150 (MQ=255)
                                             aaTCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCttat                                                                                                   <  1:1086705/150‑1 (MQ=255)
                                             aaTCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCttat                                                                                                   <  2:2910083/150‑1 (MQ=255)
                                                ctctTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTaa                                                                                                <  2:1190600/150‑1 (MQ=255)
                                                 tctTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAAt                                                                                               <  2:3055025/150‑1 (MQ=255)
                                                 tctTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAAt                                                                                               <  2:2885977/150‑1 (MQ=255)
                                                  ctTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAAtt                                                                                              >  1:4370477/1‑150 (MQ=255)
                                                   ttGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAAttt                                                                                             <  1:3742936/150‑1 (MQ=255)
                                                   ttGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAAttt                                                                                             <  1:717464/150‑1 (MQ=255)
                                                    tGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCACTAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTa                                                                                            <  2:4500527/150‑1 (MQ=255)
                                                    tGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTa                                                                                            <  1:3941398/150‑1 (MQ=255)
                                                      cgtaTGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTAAACATCAACACCAATAATAAACTGGCAAACAGTTAAATAACGCTATTACTTTTCCTGCTAAACATAGTGTAGTGCCTTATTAATTTAAc                                                                                          >  1:3834277/4‑150 (MQ=255)
                                                       ggATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACt                                                                                         >  2:542410/1‑150 (MQ=255)
                                                        gATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACtt                                                                                        >  2:1339826/1‑150 (MQ=255)
                                                            gTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTggg                                                                                    <  2:57802/150‑1 (MQ=255)
                                                            gTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTggg                                                                                    <  1:2811635/150‑1 (MQ=255)
                                                             tCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTgggg                                                                                   <  2:4935542/150‑1 (MQ=255)
                                                                   aCTGGCGGTGCTGTCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGt                                                                             <  2:1183925/150‑1 (MQ=255)
                                                                     tGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTc                                                                           >  2:5147966/1‑150 (MQ=255)
                                                                       gCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTccc                                                                         <  1:345020/150‑1 (MQ=255)
                                                                              tggctgTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGt                                                                  >  1:212073/1‑150 (MQ=255)
                                                                                 ctgTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTAc                                                               <  2:1999586/150‑1 (MQ=255)
                                                                                  tgtgGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTAcc                                                              <  1:4173810/150‑1 (MQ=255)
                                                                                    tgGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTg                                                            <  1:885297/150‑1 (MQ=255)
                                                                                      gCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGt                                                          <  2:831994/150‑1 (MQ=255)
                                                                                       cGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAATCAGTTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTa                                                         <  1:110892/150‑1 (MQ=255)
                                                                                        gTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTAc                                                        >  2:2602581/1‑150 (MQ=255)
                                                                                         tACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTAcg                                                       >  2:3557520/1‑150 (MQ=255)
                                                                                         tACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTAcg                                                       >  1:1414487/1‑150 (MQ=255)
                                                                                          aCCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTAcgc                                                      >  2:301136/1‑150 (MQ=255)
                                                                                             ttGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGAt                                                   <  2:761794/150‑1 (MQ=255)
                                                                                              tGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGAtt                                                  >  2:780986/1‑150 (MQ=255)
                                                                                                    gcgcTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGaa                                            >  1:1862391/1‑150 (MQ=255)
                                                                                                     cgcTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAAt                                           <  1:75380/150‑1 (MQ=255)
                                                                                                           aGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAAc                                     >  1:3037755/1‑150 (MQ=255)
                                                                                                           aGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAAc                                     <  1:3027491/150‑1 (MQ=255)
                                                                                                           aGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAAc                                     <  1:4359888/150‑1 (MQ=255)
                                                                                                            ggCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACg                                    <  2:4252708/150‑1 (MQ=255)
                                                                                                            ggCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACg                                    <  2:5127282/150‑1 (MQ=255)
                                                                                                             gCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGt                                   >  1:1612274/1‑150 (MQ=255)
                                                                                                              cACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTg                                  >  1:5116189/1‑150 (MQ=255)
                                                                                                               aCTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGa                                 >  2:3170668/1‑150 (MQ=255)
                                                                                                               aCTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGa                                 <  1:4218877/150‑1 (MQ=255)
                                                                                                                cTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGAc                                <  2:274531/150‑1 (MQ=255)
                                                                                                                   aaCATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGaa                             <  1:1513457/150‑1 (MQ=255)
                                                                                                                   aaCATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGaa                             >  2:4079068/1‑150 (MQ=255)
                                                                                                                      aTCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGc                          <  2:1056396/150‑1 (MQ=255)
                                                                                                                      aTCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGc                          >  1:3966043/1‑150 (MQ=255)
                                                                                                                        cAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGt                        >  1:3233469/1‑150 (MQ=255)
                                                                                                                        cAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGt                        <  1:4972145/150‑1 (MQ=255)
                                                                                                                         aaCACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTa                       >  1:1214829/1‑150 (MQ=255)
                                                                                                                              cAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCca                  <  2:1281703/150‑1 (MQ=255)
                                                                                                                               aataatAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCcac                 >  1:211001/1‑150 (MQ=255)
                                                                                                                                ataataATCTGGCAAACAGGTAAATAACGCTAGTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACATTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCcaca                <  1:3363794/150‑1 (MQ=255)
                                                                                                                                  aataaACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACAtc              >  1:463706/1‑150 (MQ=255)
                                                                                                                                    taaACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTACCTTTGGGGGACTGTACCCGAATTGATACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACAtctc            >  1:4828425/1‑150 (MQ=255)
                                                                                                                                     aaaCTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCa           <  2:1694762/150‑1 (MQ=255)
                                                                                                                                        cTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACa        >  2:4828578/1‑150 (MQ=255)
                                                                                                                                         tGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACAt       >  2:2493817/1‑150 (MQ=255)
                                                                                                                                          ggCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACAtt      >  1:568289/1‑150 (MQ=255)
                                                                                                                                            cAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTAt    <  1:3764932/150‑1 (MQ=255)
                                                                                                                                             aaaCAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATg   <  1:4712840/150‑1 (MQ=255)
                                                                                                                                             aaaCAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATg   >  2:1438266/1‑150 (MQ=255)
                                                                                                                                             aaaCAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATg   <  1:3466773/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCTTACGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGA  >  CP009273/4406408‑4406699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑